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김병각,최응칠,박희주,김하원,심미자,우명식,박완희 한국균학회 1985 韓國菌學會誌 Vol.13 No.3
To find antitumor constituents in the shake cultured mycelia of Agaricus campestris, the mycelia were extracted with hot water. Purification of the extract was carried out by ethanol precipitation and by ion exchange chromatography using DEAE-Sephadex A-50. Each fraction obtained during the purification procedure was examined for antitumor activity against sarcoma 180 in ICR mice. The antitumor fraction C was isolated. It showed 56.1% inhibition ratio at a dose of 20 ㎎/㎏/day and consisted of a polysaccharide moiety (45%) and a protein moiety (18%). The polysaccharide was analyzed by G.L.C. and found to contain mannose(42.0%), glucose (25.5%), xylose (16.6%), fucose and galactose. The protein moiety was composed of 17 amino acids. The antitumor fraction A showed immunopotentiating activity by accumulating peritoneal macrophages and by increasing plaque-forming cells in mice.