Lactobacillus acidophilus가 생산한 항균물질(抗菌物質)에 관한 연구(硏究)

김동신,Kim, Dong-shin 대한수의학회 1984 大韓獸醫學會誌 Vol.24 No.2

The research was conducted(1) to confirm the agent(s) responsible for the antimicrobial activity contained in the fermented tomato juice with L. acidophilus(2) to extract and purify the antimicrobial agent(s)(3) to find the biological, physical and chemical properties of the agent(s). The following results were obtained and summarized as followings; 1. The agent responsible for the inhibitory activity was confirmed by both well assay method using fermented tomato juice with L. acidophilus and turbidimetric technique using the cell-free filtrate or neutralized filtrate of tomato acidohilus culture and found exerted antimicrobial agent other than lactic acid. 2. The procedures of purification : The isolation and purification of antimicrobial agent from the lyophilized acidophilus tomato culture were carried out by (1) methanol extraction (2) acetone extraction, (3) Sephadex G-50 gel filtration (4) paper chromatography and (5) thin layer chromatography. 3. The biological, physical and chemical properties of antimicrobial agent: The biological, physical, chemical properties of the purified antimicrobial agent were: (1) The antimicrobial activity was strong against test organisms; Bacillus subtilis(ATCC 6633), Escheichia coli(ATCC 25922), Staphylococcus aureus(ATCC 167), Pseudomonas fluorescens(KFCC 32394), Proteus vulgaris and Shigella dysenteriae. (2) The pH value of the agent was 2.0 and the inhibitory activity was lost when it was neutralized at 7.0 of pH and the agent was heat stable at $121^{\circ}C$ for 60 minutes. (3) The ultraviolet light absorption spectra of methanol-acetone extract and TLC fraction exhibited a maximum absorption at 260nm and 224nm respectively. (4) The most purified agent from TLC plate increased about 130-fold in activity. (5) The agent isolated from TLC plate was free from $H_2O_2$ or lactic acid. 4. Bioautographic assy: By means of bioautography of the agent on silica gel of TLC plate a strong inhibitory activity against B. subtilis was demonstrated. 5. Mass spectrometry: The agent obtained from TLC plate was analyzed by mass spectrometry which show the parent peak at m/e 264 suggesting the molecular weight of the compound and molecular group such as [$C_2H{^+}_4$], [CO], [CH=NH], [$C_3{H^}4_7$], [$\begin{array}{rcl}O\\{\parallel}\\CH_3-C\\\end{array}$], [$C_6-H{^+}_{11}$], [$C_5H{^+}_{11}$], [$\begin{array}{rcl}O\\{\parallel}\\C_5H_7-C^+\\\end{array}$] were suggested.

Lactobacillus acidophilus를 이용한 발효유제품

김동신,Kim, Dong-Sin 한국유가공학회 1981 한국유가공기술과학회지 Vol.1 No.1

Lactobacillus acidophilus를 이용한 발효유제품

김동신,Kim, Dong-Sin 한국유가공협회 1981 牛乳 Vol.2 No.4

다중 방송 메쉬 상에서 최근접쌍을 구하는 최적 병렬 알고리즘

김동신(Dongshin Kim),유관우(Kwanwoo Ryu) 한국정보과학회 1998 정보과학회논문지 : 시스템 및 이론 Vol.25 No.10

평면상의 n개의 점들의 집합 S={q₁, q₂, . . . , q_n}가 주어질 때, 이들 n개의 점들중 서로간의 거리가 가장 가까운 두점을 최근접쌍(the closest pair)이라 한다. 본 논문에서는 n×n 다중방송메쉬(Mesh with Multiple Broadcasting, MMB) 상에서 최근접쌍을 찾는 최적 시간 병렬 알고리즘을 제시한다. [9]에서는 이 문제보다 더 일반적인 문제인 각각의 점에서 가장 가까운 점들을 찾는 ANN(All-Nearest-Neighbor) 문제를 해결하는 알고리즘이 제시되어 있는데, 이는 아주 복잡한 알고리즘이며, 또 [9]에서는 이 보다 더 쉬운 방법으로 최근접쌍을 찾을 수 없었다고 한다. 본 논문에서는 이 최근접쌍을 찾는 문제를 해결하는 보다 더 간단한 최적 알고리즘을 제시한다. The Closest Pair problem can be defined as follows: Given a set S={q₁, q₂, . . . , q_n} of n points in the plane, find a pair of points in S that are closest to each other. In this paper we develop a time-optimal parallel algorithm for solving the CP problem on the n×n mesh with multiple broadcasting(MMB). An optimal algorithm to solve a more general problem of finding a nearest neighbor for every given point in the MMB has been already presented in [9]. However, this algorithm is very complicated, and in [9] they said that they could not construct a simpler algorithm for solving the closest pair problem. Hence, we present in this paper a much simpler algorithm for finding a closest pair among the given n points in the plane.

5단계 교수학습법을 적용한 창조수업모형 연구

김동신(Kim, Dong-Sin) 창조교육학회 2013 창조교육논총 Vol.15 No.-

In the meantime, the creation of a joint training and educational activities of the Institute for Creative Education humans can see the theoretical foundation has been completed. But class field theory to practice in the field by applying several reality is that the environment is not easy on the draw. Faced with these difficulties, the situation was exhilarating encounter good news. It was none other than the government's economic policies. Park Geun-hye is the government's national agenda, the creative economy, the creative economy is a key department to promote the future creation of a national vision of Sciences, including the creation of government enough to be represented by the word creation days is really the best mantra seems general trend. Creative Education Association as a member of the can not be good news indeed. What is the creative economy and people's imagination and creativity in science and technology and by integrating ICT creates new industries and markets, and by strengthening existing industries to create a new economic strategy is a good job. Education is the creation of human nature, creativity, and expression of creativity that is the potential for students by students to complete an individual's personality and the family, society and human well-developed countries, and the creation of a new culture and values​​, and ultimately create a new history education is to train human. Creative Creation trained humans is discharged through the school community will be further created the leading role of social imagination and creative concrete realization of human education faculty learning how to step 5 lessons to apply to the field to practice constantly, and now the effort made up of incomplete attempts will be introduced.

강화학습 기반 비활성 영역 패딩 기술

김동신(Dongsin Kim),우딘 쿠툽(Kutub Uddin),오병태(Byung Tae Oh) 한국방송·미디어공학회 2021 방송공학회논문지 Vol.26 No.5

변형된 메쉬상에서 최근접쌍을 구하는 알고리즘

김동신(Dong Shin Kim),유관우(Kwan Woo Ryu) 한국정보과학회 1996 한국정보과학회 학술발표논문집 Vol.23 No.1A

최근접쌍 찾기 문제(CP(closest pair)문제)란 평면상에 놓인 점들의 집합 S=(q₁, q₂, ..., q_n)가 주어져 있을때 최근접쌍을 찾는 문제이다. CP문제는 VLSI 설계, 컴퓨터 그래픽스(computer graphics), 패턴 인식(pattern recognition), 이미지 처리(image processing), 실시간 응용(real-time application)등의 분야에서 많이 고려되는 문제이다. 본 논문에서 우리는 MMB(mesh with multiple broadcasting)라는 변형된 메쉬에서 최근접쌍을 찾는 최적 알고리즘을 제시한다. 이에 대해선 논문[10]에서 오픈문제로 제시되어있다. 그리고 EMMB(enhanced mesh with multiple broadcasting)라는 새로운 변형된 메쉬 모델을 제시해 EMMB상에서는 CP문제가 훨씬 더 쉽게 풀릴 수 있음을 보인다.

한일 옥외광고물 가이드라인 비교를 통한 옥외광고물 가이드라인 개선방안에 대한 연구

김동신(Kim Dong Shin) 한국디자인학회 2008 한국디자인학회 학술발표대회 논문집 Vol.- No.-

아임계수에 의한 양파 추출물과 양파즙 시제품의 품질 특성

김동신(Dong-Shin Kim),임상빈(Sang-Bin Lim) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.7

본 연구에서는 페놀화합물 함량이 높은 양파즙을 제조할 목적으로 추출온도와 시간을 달리하여 아임계수로 시료의 균일성을 담보하기 위하여 생양파 대신에 동결건조 양파를 추출하여 개별 페놀화합물 함량과 항산화 활성을 측정하였고, 최적 추출조건에서 생양파 과육과 껍질의 아임계수 추출물을 혼합하여 양파즙 시제품을 제조하여 시판품 3종과 품질특성을 비교 분석하였다. 동결건조 양파분말의 아임계수 추출물(120°C/10분)은 열수 추출물(100°C/10분)보다 전체 페놀화합물 함량이 1.50배 높았으며, quercetin, quercetin-3,4’-diglucoside, quercetin-4’-glucoside, protocatechuic acid의 함량은 각각 2.19배, 1.28배, 1.52배, 1.31배 높았다. 총페놀(TPC)과 총플라보노이드(TFC) 함량은 아임계수 추출물(120°C/10분)이 열수 추출물(100°C/10분)보다 각각 1.48배와 1.53배 높았다. DPPH 자유라디칼 소거 활성과 FRAP는 추출온도와 추출시간의 증가에 따라 증가하였으며, 아임계수 추출물(120°C/30분)은 열수 추출물(100°C/10분)보다 각각 1.82배와 1.63배 높았다. 생양파와 생양파 껍질의 아임계수 추출물을 88:12의 비율로 혼합하여 양파즙 시제품을 제조해 시판품 3종과 품질 특성을 비교하였다. 전체 페놀화합물과 quercetin의 함량은 시제품이 시판품보다 각각 3.1배와 5.4배 높은 반면에 시제품의 TPC, TFC, DPPH 자유라디칼 소거능, FRAP는 시판품보다 각각 1.22배, 1.21배, 1.37배, 1.15배밖에 높지 않았는데, 시판품은 페놀화합물 이외의 다른 성분들에 의한 영향이 높았다. 열손상 지표 물질인 5-HMF의 함량은 시판품이 19.50 mg/100 mL로 시제품의 0.23 mg/100 mL보다 84.7배 높았다. 결론적으로 아임계수를 이용하면 120°C/10분 처리로 페놀화합물의 함량과 항산화 활성이 높고 열에 의한 손상이 적은 우수한 양파즙을 제조할 수 있었다. Freeze-dried onion was extracted with subcritical water at different temperatures and times, and the individual phenolic compounds, total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity [2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant power (FRAP)] were measured. The total individual phenolic compounds in the subcritical water extract (SWE) (120°C/10 min) was 1.50-fold higher than that in the hot water extract (HWE) (100°C/10 min). The quercetin, quercetin-3,4’-diglucoside, quercetin-4’-glucoside, and protocatechuic acid contents in the SWE (120°C/10 min) were 2.19-, 1.28-, 1.52-, and 1.31-fold higher, respectively, than those in the HWE. TPC and TFC were also 1.48- and 1.53-fold higher in the SWE (120°C/10 min), respectively, than those in the HWE. DPPH and FRAP increased with increasing extraction temperature and time, and were 1.82- and 1.63-fold higher in the SWE (120°C/30 min), respectively, than those in the HWE. A trial product (TP) of onion juice was made with subcritical water extracts of raw onion and onion skin (88:12) to prepare onion juice with a high phenolic content, and its quality was compared with that of the commercial product (CP). The total individual phenolic compounds and quercetin levels in the TP were 3.1- and 5.4- times higher than those in the CP, respectively. On the other hand, TPC, TFC, DPPH, and FRAP in the TP were only 1.22, 1.21, 1.37, and 1.15 times higher than those in the CP, and other chemical compounds in the CP affected those properties. The 5-hydroxymethylfurfural content, which is a heat damage index, of the CP was 84.7 times higher than that of the TP. These results indicate that onion juice made by subcritical water extraction at 120°C/10 min was high in phenolic compounds and high in antioxidant activity without significant heat damage.

샤시 통합 제어 시스템

김동신(Dong Shin Kim) 한국자동차공학회 2007 오토저널 Vol.29 No.3