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전남 서부지역 설사 자돈에서 분리한 Clostridium perfringens에 관한 연구
김내영 ( Nae Young Kim ),오은희 ( Eun Hee Oh ),홍갑표 ( Khap Pyoe Houng ),강규칠 ( Gu Chil Kang ),정인호 ( In Ho Chung ),박석준 ( Suk Joon Park ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.2
Eighteen strains of Clostridium perfringens were isolated from the piglets with hemorrhagic enteritis. The characteristics of the outbreaks, clinical signs and lesions were examined. The biochemical properties, type of toxins and susceptibility to antimicrobial agents against the isolates were investigated. 1. The incidence of diarrhea was appeared in 97(22.4%) of 432 piglets examined. 2. The isolation rate of Cl perfingens from the 97 diarrheal faeces were 18.5%(18 strains) 3. The population of Cl perfringens in feces were ranged 108-9cfu/g in 5(32.5%) and 103-7cfu/g in 13(67.4%) of 18 samples. 4. The toxin type of the 18 isolates investigated by mouse inoculation test was all type C strains of Cl perfringens. 5. As a results of antimicrobial susceptibility test, 18 isolates were higly susceptible to cephalothin, tetracycline and penicillin.
광주와 전남지역 사육견의 개심장사상충(Dirofilaria immitis)감염률 조사
정대영 ( Dae Young Chung ),김희정 ( Hee Jeong Kim ),이태욱 ( Tae Uk Lee ),김내영 ( Nae Young Kim ),이정치 ( Jeong Chee Lee ),신성식 ( Sung Shik Shin ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.1
We have investigated the prevalence of canine heartworm (Dirofilaria immitis) infection among 100 mixed-breed dogs raised in Chonnam Province(Koheung, Posung, Changheung, and Hwasoon) and 100 dogs randomly selected from visiting dogs to the College of Veterinary Medicine, Chonnam National University for routine physical examination. Blood samples taken from dogs were examined for the presence of Dirofilaria immitis microfilaria by the modified Knott`s test, and an antigen test which was based on the sandwich ELISA(DiroCHEK®, Synbiotics, Inc., San Diego, California, USA) was used to detect the adult heartworm-specific antigen from serum samples. The results were then compared with those of the modified Knott`s test. Five(2.5%) of the 200 examined dogs were microfilaria-positive, while 8 dogs(4%) were anti-gen-positive, which suggested that the antigen test was more sensitive than the microfilarial test in detecting heartworm infection. All dogs that were microfilaria-positive were also antigen-positive. The place of origin of the heartworm-positive dogs were of Naju (4/26, 15.4%), Koheung(3/32, 9.4%), and Hwasoon (1/19, 5.3%), while none of dogs raised in Kwangju, Posung, and Changheung areas were infected with the parasite. Although not significant, more infected dogs were observed in older ages, while the difference in infection rate between male (4/98, 4.1%) and female (4/102, 3.9%) was not recognized. Our results indicated that a relatively low infection rate was observed in dogs raised in Kwangju and Chonnam area compared to the infection rate reported previously in other areas of South Korea.
음성대조 염색법을 이용한 설사 한우송아지 분변내 바이러스양 입자 검색
임종수 ( Jong Su Lim ),강춘원 ( Chun Won Kang ),이태욱 ( Tae Uk Lee ),김내영 ( Nae Young Kim ),정용운 ( Yong Wun Jeong ),강문일 ( Mun Il Kang ),한동운 ( Dong Un Han ),최현성 ( Hyon Sung Choi ),이채용 ( Chai Yong Lee ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.1
Presently, viral isolation in the diarrheal feces can be reached by many tools such as fluorescent antibody test(FA), negative contrast electron microscopy(NCEM), virus neutralization test, cell culture, and so on. The purpose of the study was to aimed at the establishment of simplified NCEM technique which can be efficiently applied for diarrheal feces and also the understanding on prevalence of viral-induced diarrhea in calves. One hundred fourty-seven korean indigenous calves with diarrhea were examined to their feces by the modified NCEM. Among them, 98(66.7%) were confirmed to have one or more viruses in feces. The viruses detected were identified as rotavirus(33.3%), coronavirus(16.3%), togavirus(10.2%) and herpesvirus(0.7%). Ten cases of combined viral infection were consisted of 8 with rotavirus + coronavirus, one with rotavirus + togavirus and one with rotavirus + herpesvi-rus. Dirrheal types could classified by yello-wish watery(44.9%), blood-tinged(19.7%), white watery(17.7%), brownish watery(14.3%), greenish watery(3.4 %) diarrhea, respectively. Yellowish watery diarrhea(66cases) was frequently included rotavirus(31.8%), coronavirus(15.2%), and togavirus(13.6%), respectively. Consequently, these results suggest that the modified NCEM is reliable and efficient diagnostic tool for detection of viruses in the diarrheal feces and many calves rearing in Chonnam province have been exposed to some enteric viral agents mainly including rotavirus and coronavirus.
전남도내 사육중인 젖소유래 staphylococcus aureus의 enterotoxin생선과 coagulase 아형분석에 관한 연구
박준규 ( Jun Kyu Park ),임재향 ( Jae Hyang Lim ),서영동 ( Young Dong Seo ),김내영 ( Nae Yeong Kim ),임동연 ( Dong Yeon Lim ),윤선종 ( Seun Jong Yoon ),최종성 ( Jong Sung Choi ),고홍범 ( Hong Bum Koh ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.4
Staphylococcus aureus is a causative pathogen of bovine mastitis. It is recognized as a common pathogen in human and animal and specially enterotoxin-producing strain of S aureus is a common cause of staphylococcal food poisoning in human. Various food originated raw milk, cheese, butter produced from mastitic cow causes staphylococcal food poisoning. It is difficult to treat the staphylococcal mastitis because of increasing resistance by using overdose of antibiotics. This study was conducted to investigate the enterotoxin-production and coagulase serotypes of S aureus in Chonnam province for 6 month, 1999. Also we studied the antibiotic resistant pattern with 14 types against isolates. 18(10.1%) 5 aureus were isolated from 178 raw milk samples in seven farms. and 8 strains(38%) were isolated in 21 raw milk samples which was below 500,000 somatic cells. We identify that 7(87.5%) of 8 isolates and 15(83.3%) 18 isolates produce enterotoxin. Their enterotoxin serotype was type B(66.7%), type A(33.3%) and type C(13.3%). Also 2 strains of isolates was positive to the type A and B. Coagulase serotype of isolates was 2, 3, 4, 7, arid 8. Most stains(70.6%) were serotype 2. And most strains(17 isolates, 94.4%) except one isolate was multiple resistant to the tested antibiotics.