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음성대조 염색법을 이용한 설사 한우송아지 분변내 바이러스양 입자 검색
임종수 ( Jong Su Lim ),강춘원 ( Chun Won Kang ),이태욱 ( Tae Uk Lee ),김내영 ( Nae Young Kim ),정용운 ( Yong Wun Jeong ),강문일 ( Mun Il Kang ),한동운 ( Dong Un Han ),최현성 ( Hyon Sung Choi ),이채용 ( Chai Yong Lee ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.1
Presently, viral isolation in the diarrheal feces can be reached by many tools such as fluorescent antibody test(FA), negative contrast electron microscopy(NCEM), virus neutralization test, cell culture, and so on. The purpose of the study was to aimed at the establishment of simplified NCEM technique which can be efficiently applied for diarrheal feces and also the understanding on prevalence of viral-induced diarrhea in calves. One hundred fourty-seven korean indigenous calves with diarrhea were examined to their feces by the modified NCEM. Among them, 98(66.7%) were confirmed to have one or more viruses in feces. The viruses detected were identified as rotavirus(33.3%), coronavirus(16.3%), togavirus(10.2%) and herpesvirus(0.7%). Ten cases of combined viral infection were consisted of 8 with rotavirus + coronavirus, one with rotavirus + togavirus and one with rotavirus + herpesvi-rus. Dirrheal types could classified by yello-wish watery(44.9%), blood-tinged(19.7%), white watery(17.7%), brownish watery(14.3%), greenish watery(3.4 %) diarrhea, respectively. Yellowish watery diarrhea(66cases) was frequently included rotavirus(31.8%), coronavirus(15.2%), and togavirus(13.6%), respectively. Consequently, these results suggest that the modified NCEM is reliable and efficient diagnostic tool for detection of viruses in the diarrheal feces and many calves rearing in Chonnam province have been exposed to some enteric viral agents mainly including rotavirus and coronavirus.