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      • FMH 성능 만족을 위한 Countermeasure 설계변수 최적화

        최영윤(Youngyoon Choi),김기윤(Giyun Kim),이진의(Jinui Lee) 한국자동차공학회 2014 한국자동차공학회 부문종합 학술대회 Vol.2014 No.5

        Vehicle Manufacturers effort to meet safety regulation and improve performance for vehicle sale in each country. Specially, Regulation related to interior part is getting important because driver or passenger have to interact directly in accident. NHTSA announced that FMVSS201U regulation have to be met for sale in US. To meet the FMVSS201U regulation, various energy absorbers need to play a role as a countermeasure between roof and Interior headliner. And the shape and material type of energy absorber should be cost effective and need to be optimized. So, in this paper, we studied with the feasible design variables and shows design of energy absorber in a point of the cost effective and meeting FMVSS201U regulation.

      • FMH 성능향상을 위한 B-Pillar Upper Roof 부위 Countermeasure 형상 최적화

        임종수(Jongsoo Lim),김기윤(Giyun Kim),이재우(Jaewoo Lee) 한국자동차공학회 2013 한국자동차공학회 부문종합 학술대회 Vol.2013 No.5

        Energy absorbers play important roles as a countermeasure to meet legal requirement of FMVSS201, which regulates passenger’s head injury on side collision accident. It is important to develop countermeasure strategy that improve head injury performance and robustness as well because the FMVSS201 testing shows low repeatability and reproducibility on regular testing conditions. So this paper investigates the contributiveness of each control factor such as crush load and geometry of two types of low density foams in a standpoint of head injury performance and robustness, against the variation of impact test velocity and HOH (Hit on Head) location applying DFSS optimization process.

      • Amylase 特異的인 抗體의 生産

        강미란,이정민,유형진,김기윤,권무식 성균관대학교 생명과학자원연구소 1997 生命資源科學硏究 Vol.4 No.1

        The advent of immunochemistry expedites the development of a number of important procedures for gene cloning, disease control (diagnosis, treatment) and biochemical analyses in animal systems. These procedures can be applicable to plant systems : immunochemical detection of biopolymers (proteins, carbohydrates, growth hormones, and pathogenic virus particles). It is not quite difficult to purify various palnt immunogens. However, immunochemical screening methods for specific substances of plants are scarce, so that these procedures for each substance are to be developed. During the cereal germination, amylase converts the starchy endosperm of the seed to nutrient that support the growing seedling. α-Amylase and β-amylase hydrolyze internal α-1,4 glycosidic linkages in starch and dextrin. They produce α-maltose and β-maltose respectively. Their gene expression is regulated by gibberellic acid (negative regulator) and abscisic acid (positive regulator). In this study, α-amylase (from barley malt) protein was analyzed with SDS-PAGE. The molecular weight of α-amylase was about 60,000 daltons. α-Amylase protein was used as immunogen to produce antiα-amylase antiserum in a rabbit. The immunogen was mixed with an equal volume of Freund's complete adjuvant, and the mixture was hypodermically injected to the hideback (30 spots) and paws (2 spots) of the rabbit. The immunization was performed with two week interval. The dosage of the immunogen was decreased by half, and incomplete adjuvant was substituted for the complete one from the second immunization. The serum was prepared from the blood, and immunoreactivity of the serum was examined by dot-blotting and western blotting using the GARHRP indirect immunoassay kit. It was found that the anti α-amylase antiserum recognized nano-gram quantity of antigen under these experimental conditions. The antiserum will be used for the development of immunochemical assay to detect the time of the seed germination.

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