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      • 한국사람의 뇨중식염배설량과 혈압과의 상호관계에 관한 연구

        김구자(Kim, Ku-Ja) 대한생리학회 1974 대한생리학회지 Vol.8 No.2

        In oder to study the correlation between daily urinary output of sodium chloride and blood pressure, twenty four hour urine samples were collected from 224 cases (70 male and 154 female) of healthy Koreans whose age varied from 18 to 70 years old. The volume and concentration of sodium, chloride and potassium and total nitrogen were measured, along with the resting blood pressure. Results obtained are summarized as follows; 1. Daily urinary output was increased as a function of age. However, daily urinary output per unit sulface area was maintained at approximately 800 to 900 ml/m<sup>2</sup> in all age groups of male and it increased as a function of age in female groups. There was no significant difference between male and female. 2. The daily urinary sodium concentration was decreased gradually acceding to age in both sexes. Daily excretion of sodium was constant regardless of age in both sexes but especially high in 25-39 year female age group, which was slightly greater in males than in females. 3. The daily urinary chloride concentration was at approximately 250 meq/L in all age groups of male and which decreased as a function of age in females. 4. Hence the daily urinary output of sodium chloride was constant in all age groups of males which increased as a function of age in female groups. However, daily excretion of sodium chloride per unit sulface area was maintained at approximately 11 gm/m<sup>2</sup> in males and which increased as a function of age in females. 5. The daily urinary excretion of potassium was constant regardless of age in both sexes. 6. Urinary K/Na ratio was maintained at approximately 0.27 in males and 0.33 in females. 7. The daily urinary output of total nitrogen amount was approximately 8 ~ 10 gm in males and 7 ~ 9 gm in females. However, daily urinary output of nitrogen per unit sulface area was constant regardless of age in both sexes. 8. The systolic blood pressure was increased gradually according to the age in both sexes and was higher for males than females under 40 years of age. However, there was no significant difference between both sexes in ages over 40 years. 9. Quantitative comparisons indicated that daily urinary output and sodium chloride excretion are higher while daily potassium output, nitrogen excretion and urinary K/Na ratio are significantly lower among Koreans than a among Occidentals. These findings suggest that average Koreans live on low-protein and high-salt diet throughout their livers. Statistical result obtained may he summarized as follows; 10. The relation between blood pressure and sodium concentration of urine. The correlation between systolic blood pressure and sodium concentration was negatively associated for both sexes and the correlation coefficient was significant for females γ<Sub>1</sub>=-.19<-γ<Sub>152</sub>, <sub>0.05</sub>=-0.159) and it was not significant for males (γ<Sub>1</sub>=-.19>-γ<sub>68</sub>, <sub>0.05</sub>=-0.232) but may be due to the sample size for males. The correlation between diastolic blood Pressure and sodium concentration was negatively associated for both sexes and the correlation coefficient was significant for males (γ<Sub>1</sub>=-.37<-γ<sub>68</sub>, 0.05=-0.232) and the relation was not significant for females (γ<Sub>1</sub>=-.11>-γ<sub>152</sub>, <sub>0.05</sub>=-0.159).

      • KCI등재
      • KCI등재
      • 수종의 알레르기 관련 약물이 흰쥐의 복강내 비만세포에서 Hyaluronidase 및 히스타민 유리에 미치는 영향

        유신애,김구자,하종식,Yoo, Shin-Ae,Kim, Ku-Ja,Hah, Jong-Sik 대한생리학회 1988 대한생리학회지 Vol.22 No.2

        Type I allergic reaction and it's related clinical manifestations are known to occur by the effects of various chemical mediators. These chemical mediators are released from circulating basophils and tissue mast cells, which become 'sensitized' through the binding of antigens and antibodies of the IgE type to their cell surface receptors. Efforts to elucidate the mechanism of the release of these mediators, especially that of histamine, have been persued for years. The mechanism is not yet clarified at the present time. Recent reports of hyaluronidase, an enzyme known to be involved in the tissue inflammatory process, as possible participant in type I allergic reaction, initiated this study. Relationships between the hyaluronidase activity and histamine release from the sensitized rat peritoneal mast cells were investigated. Also anti-allergic agents, tranilast and disodium cromoglycate, along with known histamine releasers, morphine and compound 48/80, were used to observe the inhibitory and stimulatory effects of these substances on the hyaluronidase activity as well as histamine release from the rat mast cells. The results obtained are summarized as follows: 1) Hyaluronidase activity and histamine release from sensitiaed rat peritoneal mast cells started to increase on the 4th day of postsensitization. Hyaluronidase activity reached it's peak value on the 7th day of postsensitization and that of histamine release on the 14th day of postsensitization. 2) Hyaluronidase activity and histamine release from sensitized rat peritoneal mast cells, pre-treated with tranilast revealed significant decrease in comparison with those of non-treated cells. 3) Hyaluronidase activity and histamine release from sensitized rat peritoneal mast cells, pre-treated with tranilast, followed by morphine injection, revealed significant increase in comparison with those of tranilast treated cells. 4) In vitro study of hyaluronidase activity and histamine release from un-sensitized rat peritoneal mast cells, using morphine and compound 48/80 as activators, revealed significant increase compared to those of non-activator used cells. 5) In vitro study of hyaluronidase activity and histamine release from un-sensitized rat peritoneal mast cells, pre-treated with tranilast and disodium cromoglycate, using confound 48/80 and morphine as activators revealed significant decrease in comparison with those of tranilast and disodium cromoglycate treated cells. From above results, participation of enzyme hyaluronidase in the process of histamine release from sensitized rat pertioneal mast cells, could be suggested. It was also quite evident that the clinically used anti-allergic agents, tranilast and disodium cromoglycate, have significant inhibitory function on the hyaluronidase activity and histamine release from sensitized rat peritoneal mast cells, while morphine significantly increased the hyaluronidase activity and histamine release from sensitized rat peritoneal mast cells.

      • 인공적혈구의 제조 및 이용

        하종식,조응행,김구자,Hah, Jong-Sik,Cho, Eng-Haeng,Kim, Ku-Ja 대한생리학회 1990 대한생리학회지 Vol.24 No.1

        Hemoglobin was purified from the outdated human red blood cells. Phospholipids were purified from egg yolk and stored in chloroform. The artificial red blood cells (hemosome) were prepared by encapsulation of hemoglobin with phospholipid mutilayer using rotary vacuum evaporator. The shape and size of hemosomes were measured by phase contrast microscope and image analyzer. The function of hemosomes was tested by measuring oxygen dissociation curve using blood gas analyzer. In order to test whether hemosomes are useful as blood substitute they were infused into rats of which one third of total blood were drawn. The results obtained are summarized at followings. 1) Hemosomes were spherical shape and their mean diameter was 0.7 um. 2) Oxygen dissociation curve of hemosomes showed the same figure as that of normal red blood cells. 3) All rats given 1/3 transfusion with hemosomes survived until sacrificed whereas three of four rats given 1/3 transfusion with saline died within 1 hour and the rest of them died within 24 hours.

      • KCI등재

        19세기 이후 여자 저고리 형태에 관한 실증적 연구

        이태옥(Lee Tae-Ok),김구자(Kim Ku-Ja) 한복문화학회 2009 韓服文化 Vol.12 No.1

        This study was to examine the change in the shape and was to analyze the characteristics of the Jeogori according to the seven historical periods from 1864, the end of the Yi Dynasty to 1999, the end of the 20th century. The Jeogori samples were collected from those of between 1864 and 1999. Jeogori samples were 687 in total. 12 items of measurement were measured. The data were analyzed by ANOVA and Duncan’s multiple range test as post hocand Pearson’s correlation using the SPSS Windows 12. statistical package. The summary of this study is as follows: 1. There were mush change in the Jeogori measurements depending on the historical periods. Especially, the F-test on the length items such as the length of the back, the length of the front, the length of the left fly front strap, and the length of the left front collar were significantly different on the level P< 0.001 among the seven periods 2. The correlation coefficients of the length of the back and the length of the front were more than 0.90 and were highly correlated. The correlation coefficient between the length of the back and the length of the left fly front strap was 0.92 and highly correlated. In addition, it was observed that the armhole became larger, while the sleeve width became wider and the length of the collar became longer. 3. Analyzing the characteristics of Jeogori for each period, 1) The Jeogori in the end of the Yi Dynasty (1864~1910) had the shortest length and tight width with a very short length under the armhole. 2) The characteristics of the Jeogori in the period of Japan’s colonial rule (1910~1945) were that the Jeogori length was so long that it almost reached to the waist, and the length under the armhole was also long with a wider collar. 3) The Jeogori in the period of recovery afterliberation(1946~1960) had a length reaching down to the waist, and the length under the armhole was also long with a wider width. 4) The characteristics of the Jeogori in the period of industrialization were that the length of Jeogori began to be shorter than that of the period of recovery after liberation(1946~1960), and its width became the widest. 5) The characteristics of the Jeogori in the period of advanced industrialization were that the length of the Jeogori was very short and its width was the narrowest since the end of the Yi Dynasty so that it was tight to the body, and the sleeve width was wide so that the inseam the of sleeve was clearly curved. 6) To sum up the characteristics of the Jeogori in the period of economic stabilization,the Jeogori length became slightly longer, and the width also became a little wider than that in the period of advanced industrialization. 7) The characteristics of the Jeogori in the period of globalization were that the Jeogori length became slightly shorter with a narrower width than that in the period of economic stabilization.

      • KCI등재
      • KCI등재

        19세기 이후 여자 저고리 봉제 변천에 관한 실증적 연구

        이태옥(Lee Tea-Ok),김구자(Kim Ku-Ja),조우현(Cho Woo-Hyun) 한복문화학회 2010 韓服文化 Vol.13 No.1

        This study was to examine the change the sewing method of the Jeogori according to the seven historical periods from 1864, the end of the Yi Dynasty to 1999, the end of the 20th century. This study is to help preserve the pictorial records, the traditional Korean Hanbok and its ornaments, which are slowly disappearing. The Jeogori samples were collected from those of between 1864 and 1999. There were Jeogori samples 687 in total. There were 7 Jeogori types: the single-layered Jeogori, the double-layered Jeogori, the triple-layered Jeogori, the quilted Jeogori, the cotton-padded Jeogori, the single-layered Jeogori on inner vest. and the double-layered Jeogori with a triple-layered body. The data were analyzed by ANOVA and Duncan's multiple range test as post hoc and Pearson's correlation using the SPSS Windows 12. statistical package. The summary of this study is as follows: 1. The total of 687 Jeogori samples were divided into 7 periods. The period of the end of Yi Dynasty (1864-1909) had 26 specimens The period of Japan's colonial rule (1910-1945) had 165 pieces. The period of recovery after liberation (1946-1960) had 191 pieces The period of industrialization (1961-1970) had 107 samples. The period of advanced industrialization (1971 -1980) had 76 pieces The period of economic stabilization (1981-1990) had 67 pieces. And the period of globalization (1991-1999) had 55 pieces. 2. After analyzing the characteristics of the needlework for each Jeogori, it was noted that there were various methods used for sewing and processing the margin to sew UP the single-layered Jeogori. The shoulder part was the most finely stitched, and the collar part was the least finely stitched. There was no variety in the needlework method for the double-layered Jeogori, and the collar was usually attached using glue rather than sewn. The construction method of the cotton-padded Jeogori was the same as that of the double-layered Jeogori Blinded top stitches and running stitches were used to fix the cotton-padding and thecloth. The front hemline and the sleeve opening of the quilted Jsogori were constructed using various methods to process the trimmed lines. The ggaeggijeogori was constructed using double-stitching and had a seam that was not roll-stitched, and there were many cases where the collar had a sewn margin with a thin biased line. 3. For the closing of the .eogori, strings, buttons, loops, brooches and button knots wereused. Especially 66 button knots were found in the single-layered Jeogori during the period of recovery after liberation, 45 button knots in the single-layered Jeogori during the period of Japan's colonial rule, and various small patches were found to fix the loops, which were the squared shape, the regular pentagonal shape and the five-cornered shape.

      • Benzyl Alcohol이 세포막의 형태 및 Calcium 이온 이동에 미치는 영향

        이황현(Lee, Hwang-Hyun),하종식(Hah, Jong-Sik),김구자(Kim, Ku-Ja) 대한생리학회 1987 대한생리학회지 Vol.21 No.2

        Benzyl alcohol is known to have dual effect on the red blood cell shape change. At low concentration up to 50 mM benzyl alcohol transformed the shape from discocyte to stomatocyte by preferent binding to the inner hemileaflet, however, at higher concentratransformed the shape from discocyte to stomatocyte by preferential binding to the inner monolayer, however, at higher concentration above 50 mM benzyl alcohol transformed to echinocyte by affecting both monolayers. These results suggest that the effect of benzyl alcohol on the red blood cell shape and Ca<sup>++</sup> transport across cardiac cell membranes to assess the effects of the drug on the structures and functions of the biological cell membranes. The results are as follows: 1) Benzyl alcohol up to 40 mM caused progressive stomatocytic shap change of the red blood cell but above 50 mM benzyl alcohol caused echinocytic shape change. 2) Benzyl alcohol up to 40 mM inhibited both osmotic hemolysis and osmotic volume change of the red blood cell in hypotonic and hypertonic NaCl solutions, respectively. 3) Benzyl alcohol inhibited both Bowditch Staircase and Wood-worth Staircase phenomena at rat left auricle. 4) Benzyl alcohol at concentration of 5 mM increased Ca<sup>++</sup>-ATPase activity of red blood cell ghosts slightly but above S mM benzyl alcohol inhibited the Ca<sup>++</sup>-ATPase activity. 5) Benzyl alcohol at concentrations of 5 mM and 10 mM increased Ca<sup>++</sup>-ATPase activity slightly at rat gastrocnemius muscle S.R. but above 10 mM benzyl alcohol inhibited the Ca<sup>++</sup>-ATPase activity. Above results indicate that benzyl alcohol inhibit water permeability and Ca<sup>++</sup> transport across cell membranes in part via effects on the fluidity and transition temperatures of the bulk lipid by preferential intercalation into cytoplasmic monolayer and in part via other effect on the conformational change of active sites of the Ca<sup>++</sup>-ATPase molecule extended in cytoplasmic face.

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