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1
랫드에서 사염화탄소로 유발된 간손상에 대한 간조직 추출물 항독성 분획의 개선효과

김종선(Jongsun Kim),장자영(Ja Young Jang),신선희(Sunhee Shin),박동선(Dongsun Park),김은주(Eun Ju Kim),조정희(Jung-Hee Cho),황석연(Seock-Yeon Hwang),권철(Chull Kwon),권지현(Ji Hyun Kwon),황규계(Kyu-Kye Kwang),김윤배(Yun-Bae Kim) 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.3
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The detoxification effect of Liver Extract Antitoxic Fractions (LEAFs) on liver injury and dysfunction induced by carbon tetrachloride (CCl₄) was investigated. Female rats were injected intraperitoneally with CCl₄ at a dose of 1 ㎖/㎏ (20% in corn oil, 5 ㎖/㎏). LEAF-B or LEAF-P, originated from bovine and porcine, respectively, was treated subcutaneously at doses of 100, 300 or 500 ㎎/㎏ (10 ㎖/㎏) 4 hr and 30 min prior to as well as 4 hr and 20 hr after CCl₄ challenge. The rats were anesthesized with intraperitoneal injection of urethane (16.5% in saline, 6 ㎖/㎏) 4 hr following final LEAF treatment (24 hr after CCl₄ injection), and administered with bromosulphalein (BSP) solution (1% in saline, 2 ㎖/㎏). The initial biliary excretion time of BSP was recorded, and the blood concentration of BSP was quantified 21 and 46 min after intravenous administration. Also, blood biochemical parameters related to hepatic and renal injuries in accordance with histopathological findings were analyzed. The biliary excretion time of BSP was greatly delayed by CCl₄ injection, which was significantly attenuated by 300-500 ㎎/㎏ of LEAF-B and 100 or 500 ㎎/㎏ of LEAF-P. Also, CCl₄-induced delay in BSP clearance was markedly recovered by 300-500 ㎎/㎏ of LEAF-B or 500 ㎎/㎏ of LEAF-P. In addition, CCl₄-induced changes in blood biochemical markers related to hepatic and renal injuries were remarkably reversed by LEAF-B or LEAF-P in a dose-dependent manner. Such effects of LEAFs on liver dysfunction were in parallel with the histopathological findings of the liver. That is, CCl₄ caused centrilobular congestion, hepatocytic degeneration, lipid droplets and immflamatory cell infiltration, resulting in disintegration of hepatic cords. Interestingly, such lesions were attenuated by LEAFs treatment, reducing mean lesion scores from 2.30 in rats administered with CCl₄ alone to 1.75 and 1.65 in animals treated with 500 ㎎/㎏ of LEAF-B and LEAF-P, respectively. Taken together, it is suggested that LEAFs might have protective effects against hepatic and renal cytotoxicity and dysfunction including impairments of hepatic biosynthesis, biliary excretion, metabolism and filtration induced by CCl₄, and that the effecacy of LEAF-P is comparable to that of LEAF-B which is avaliable in clinics.
2
두개강내 상의세포종의 임상적 병리적 관계

이해철,김동원,권건영,이장철,손은익,임만빈,김인홍 대한신경외과학회 1994 Journal of Korean neurosurgical society Vol.23 No.9
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The clinical and pathological features were analyzed for 11 cases with intracranial ependymoma treated surgically at the Keimyung University Dongsan Medical Center during the years 1987 to 1992. Tumor histology was reviewed individually and grouped into three categories(Cate-gories I to HI) according to the pathologic grade used by Nazar, et a16'. The correlation between the pathologic grade and prognosis after surgery was investigated. There were 2 cases(18%6) with category I histology, 5(45%) with category II histology, and 4(36%) with category 111 histology. The high recurrent rate. short recurrent interval, high rate of cerebrospinal fluid seeding and poor outcome were noted in patients with category IlI histology. The authors also investigated the recurrent interval according to the degree of tumor resection. The mean recurrent interval after surgery was 12 months in cases of subtotal resection and 33 months in a case of total resection. Tumors resected subtotally showed response to radiation and chemotherapy. In conclusion, the pathologic grade and the degree of tumor resection were regarded as important prognostic factors after surgery. Aggressive surgery with chemotherapy or radiotherapy were required in the management of intracranial ependymoma.
3
백서의 중대뇌동맥 폐쇄로 유발시킨 뇌경색의 범위 측정과 경시적 변동

김인수,임만빈,이장철,손은익,김동원,김인홍,권건영 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.1
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본교실에서는 33마리의 백서를 실험동물로 하여 측두하 접근으로 중대뇌동맥을 폐쇄하여 뇌경색을 유발하고, 뇌경색의 범위측정을 뇌허혈 유발후 6시간, 24시간 및 48시간에 TTC 염색과 병리조직학적인 H & E 염색으로 하였다. 뇌허혈 유발후 뇌경색 범위는 6시간군에 비하여 24시간 및 48시간군에서 보다 현저히 증가하였으나 24시간 군 및 48시간 군간에는 별 차이가 없었다. 따라서 본 실험을 통해서 백서를 실험동물로 하여 뇌경색에 대한 실험을 시행시 뇌경색의 크기를 기준하여 실험 결과를 판정할 경우 뇌허혈 유발후 24시간까지만 실험 기간으로 하여도 무방할 것이라는 점을 알았다. TTC 염색과 H & E 염색의 상관 관계에서는 모든 실험 기간에서 높은 상관 관계를 보이나 6시간군에서는 상대적으로 낮으므로, 뇌허혈 유발후 조기, 즉 24시간 이내에는 병리조직학적 방법을 병행하여 시행하는 것이 더욱 정확성을 기할 것으로 생각되었다 In order to find out the accuracy of the quantification of the infarction area by using triphenyltetrazolium chloride(TTC) staining and to evaluate the change of the infarction size according to the duration after the ischemic insult, in a series of 33 adult rats, a surgical occlusion of the middle cerebral artery(MCA) was carried out through a small subtemporal craniotomy. 11 animals at 6 hour, 12 animals at 24 hour and 10 animals at 48 hour following the surgical occlusion of the MCA, rats were sacrificed and brain slices were obtained and stained with TTC, and hematoxyline and eosin(H & E). The size of the infarction area stained by each method was quantified by a computer image analysis system. The average percent of the infarction size(± standard error) was larger in the 24 and 48 hour groups than that of the 6 hour group(determined by TTC : 9.94±0.97 vs. 9.98±1.08 vs. 6.83±0.82%, respectively : 6 hour vs. 24 & 48 hour groups; one-way ANOVA test p<0.05 determined by H & E : 10.02±0.94 vs. 10.02±1.06 vs. 7.73±0.85%, respectively). However, there was no significant difference in the size of the infarction between the 24 and 48 hour groups. The size of the infarction area determined by either method was not significantly different in any group(TTC vs. H & E : paired t-test p>0.05), and linear regression analysis showed a significant correlation existed between the two methods in all groups. However, the degree of correlation was more prominent in the 24 and the 48 hour groups than 6 hour group(6 hour group : r=0.76, slope=0.78, y intercept=0.55;24 hour group : r=0.97, slope=1.03, y intercept=-0.78;48 hour group : r=0.98, slope=0.94, y intercept=0.42). From this study it is concluded that : 1) the evolution of the infarction size continues up to 24 hours following the arterial occlusion, and thereafter, the change of the infarction size is minimal in the rat. This data suggests that it is sufficient to evaluate the change of the infarction size up to 24 hours following the ischemic insult in the experimental study of ischemia in the rat. 2) the detection and the quantification of the cerebral infarction by using TTC staining is a reliable method after 24 hours following the ischemic insult. However, in the earlier period than 24 hours following the ischemic insult, staining with TTC coupled with histopathological H & E staining will add to the accuracy in the obtaining the quantity of the cerebral infarction in the rat.