Inula Sesquiterpene Lactone 이 식물호흡에 미치는 영향

권영명,우원식,우인근,이민제 ( Young Myung Kwon,Won Sick Woo,Lin Keun Woo ) 생화학분자생물학회 1973 BMB Reports Vol.6 No.2

In order to investigate the mode of biological action of alantolactone, its effects on the respiration and the growth of plants were examined. The rate of respiration of Chloredlu pyrenoidosa was greatly enhanced in the presence of Inula sesquiterpene lactones(alantolactone+isoalantolactone), but the growth of cells was profoundly inhibited. Although Inula sesquiterpene lactones increased the oxygen consumption of the aged potato tuber slices, no effect was observed in the case of the fresh slices. And Inula sesquiterpene lactones also inhibited the activation of the respiration of the slices during the aging process.

Inula Sesquiterpene Lactone이 식물호흡에 미치는 영향

권영명,우원식,우린근,이민재,Kwon, Young-Myung,Woo, Won-Sick,Woo, Lin-Keun,Lee, Min-Jai 생화학분자생물학회 1973 한국생화학회지 Vol.6 No.2

Alantolactone의 생물학적 작용기작을 구명하려는 목적에서 먼저 식물의 호흡과 생장에 마치는 영향을 조사하였다. Chlorella pyrenoidosa의 생장은 Inula lactone (alantolactone과 isolalantolactone)에 의하여 크게 억제되었으나, 반대로 호흡은 촉진되어서 산소소비량과 [$U-^{14}C$] glucose로부터의 $^{14}CO_2$ 생성이 모두 증가하였다. 감자에서 Inula lactone은 aged-slice의 호흡은 촉진시켰으나 생절편에 대하여는 별 영향을 미치지 못하였다. 한편 Inula lactone은 aging 과정에서 일어나는 전편의 호흡활성화를 억제할 수 있었으나 Inula lactone을 제거하연 철편의 호흡은 급격하게 증가되었다. In order to investigate the mode of biological action of alantolactone, its effects on the respiration and the growth of plants were examined. The rate of respiration of Chlorella pyrenoidosa was greatly enhanced in the presence of Inula sesquiterpene lactones (alantolactone+isoalantolactone), but the growth of cells was profoundly inhibited. Although Inula sesquiterpene lactones increased the oxygen consumption of the aged potato tuber slices, no effect was observed in the case of the fresh slices. And Inula sesquiterpene lactones also inhibited the activation of the respiration of the slices during the aging process.

Dunaliella tertiolecta 에 의한 acetate 의 이용 - TCA cycle 과 glyoxylate pathway 의 활성 조사 -

권영명(Young Myung Kwon),이민재(Min Jai Lee) 한국식물학회 1973 Journal of Plant Biology Vol.16 No.1-2

The utilization of acetate by Dunaliella tertiolecta was examined, and the detections and assays of the enzymes of the tricarboxylic acid cycle and the glyoxylate pathway were described. Acetate could not be utilized as a sole carbon source for the growth. The carboxyl carbon of acetate was incorporated more rapidly into CO_2 than the methyl carbon. It was identified that malate, succinate, citrate and etc., were accumulated when〔U-^(14)C〕acetate was supplied to the cell free homogenate. The following enzyme activities were measured; acetothiokinase, isocitrate dehydrogenase, fumarase, malate dehydrogenase and aconitase. Though isocitratase, malate synthetase, succinate dehydrogenase and oxoglutarate dehydrogenase could not be detected, ^(14)C from succinate was easily contributed to CO_2 and cell component. The evidence suggested that the glyoxylate pathway was not operative and showed that the TCA cycle was the all important pathway in the oxidation of acetate to CO_2 in Dunaliella.

Inula Sesquiterpene Lactone 이 Phaseolus unlgaris L. 의 조직변화와 생장에 미치는 영향

권영명 (Young Myung Kwon) 한국식물학회 1973 Journal of Plant Biology Vol.16 No.1-2

The inhibitory effect of Inula sesquiterpene lactones on the growth of Phaseolus vulgaris was tested and the abnormality of the stem organization caused by the lactones was also examined. The longitudinal growth of the young stem and the expansion of the young leaf were stopped by the application of the lactones. However, this inhibitory effect was appeared and strictly restricted within the treated area. So the young shoot was observed for possible bending as a result of the unilateral application of the lactones. When the application of the lactones into the medium, the growth of the plant was entirely repressed. However, the growth of shoot and re-initiation of root were started after the plant was transfered to the lactone free medium. And partial reversal of inhibition of the stem growth was achieved by the additions of gibberelline and the lactones.

Dunaliella tertiolecta 의 포도당산화와 산화효소계 (1) - Whole cells 과 cell - free systems 에 의한 14C - glucose 의 산화

권영명 (Young Myung Kwon) 한국식물학회 1969 Journal of Plant Biology Vol.12 No.2

Dunaliella tertiolecta did not show any increase in respiration rate when supplied with glucose, glycerol, sucrose, L-alanine, acetate, pyruvate and succinate. This was in contrast to Chlorella pyrenoidosa, which, under identical conditions, showed significant increase when supplied with glucose or acetate but not with the other compounds. Production of ^(14)CO_2 from added ^(14)C-glucose in D. tertiolecta was lower than the other ^(14)C-labelled substrates: L-alnine, glycerol, succinate, but higher than ^(14)C-sucrose addition. And it was also lower than C. pyrenoidosa experiments which were added ^(14)C-glucose as a substrate. Light reduced amounts of labelled carbon dioxide from ^(14)C-glucose or ^(14)C-acetate and increased incorporation of ^(14)C from the substrates to cell materials in either D. tertiolecta or C. pyrenoidosa. The contribution of ^(14)C from ^(14)C-glucose to ^(14)CO_2 in cell-free system of D. tertiolecta were much higher than in whole cell suspension. It was contrast to C. pyrenoidosa which were showed reduction of ^(14)CO_2 production in cell-free systems than whole cell suspensions. when cell-free systems of D. tertiolecta and C. pyrenoidosa were supplied with ATP, NAD, NADP or/and hexokinase, it was remarkably increased production of ^(14)CO_2 from the substrates than the control. It was concluded that the low ability of D. tertiolecta to metabolize glucose were caused by the impermeability of the cell membrane to glucose and were not due to deficiencies of enzyme systems concerning glucose metabolism. In the cell-free systems, it seemed to be more active pentose phosphate pathway than glycolytic pathway in D. tertiolecta.

Alantolactone 이 식물생장에 미치는 영향과 L - Cysteine 과의 상호작용

권영명 (Young Myung Kwon) 한국식물학회 1974 Journal of Plant Biology Vol.17 No.1

Inhibitory effect of alantolactone and isoalantolactone was shown in Avena straight growth test and in the formation of adventitious root in Phaseolus seedling. However, di-, and tetrahydroalantolactones were given no effect on the elongation and the rooting. Inhibitory effect of alantolactone could partly be removed by cysteine, cystine, and reduced glutathione. The plant materials were made less sensitive to alantolactone by the pretreatment of cysteine, but cysteine supplied after the treatment of alantolactone brought about no effect on the action of alantolactone. A new spot was shown on TLC plate from the mixture of alantolactone and cysteine, indicating that alantolactone can be inactivated by cysteine, not cystine, without any biological processes.

Cyclic - AMP 와 탄닌이 지베레린으로 유도되는 Amylase 생합성에 미치는 영향 - 2. Amylase

권영명(Young Myung Kwon),민경희(Kyung Hee Min),이진범(Chin Bum Lee),이춘환(Choon Hwan Lee) 한국식물학회 1978 Journal of Plant Biology Vol.21 No.1-4

The effect of tannic acid on GA_3 and cyclic-AMP promoted amylase induction in barley aleurone layers was examined. Of a variety of adenine compounds, only cyclic-AMP and ADP showed significant activity, and these activities were promoted by addition of theophylline to the icubation medium. When aleurone layers of barley endosperm tissues were incubated with GA_3 in the presence of tannic acid, the amylase activity in the incubation medium was reduced. Cyclic AMP induced amylase activity was also reduced by addition of tannic acid. The cyclic-AMP response promoted was more sensitive to tannion inhibition than GA_3 response. The inhibitory effect of tannic acid showed reversibility by addition of higher concentration of GA_3 or cyclic-AMP. The tannic acid effect on GA_3 response was also recovered by addition of a higher concentration of cyclic-AMP. Experiment with polyacrylamide disc electrophoresis showed differeent isozyme patterns according to the additions in the incubation medium. Inhibitory effects of decursinol and coumarin was compared with that of tannic acid. They showed different zymogram patterns.

벼의 생육시기에 따른 일부 광호흡효소의 활성변화

권영명(Young Myung Kwon),이진범(Jin Bum Lee),이순희(Sun Hi Lee),조영동(Young Dong Cho) 한국식물학회 1979 Journal of Plant Biology Vol.22 No.3

Catalase, glycolate oxidase, hydroxypyruvate and NADPH-glyoxylate reductases activities in cell free extracts from leaves of 3 cultivars, Suwon 264, IR 36 and Jin Heung of rice plants were studied at different growing stages. Catalase and glycolate oxidase shows inclining activities toward the maximum vegetative growth whereas declining activities in either the enzymes were noticed during the maturing stage. After the photopeiodic condition exhibit increasing hydroxypyruvate and NADPH-glyoxylate reductases activities with time until maturing stage. No significant differences were found in the enzyme activities, and in analytical data of nitrogen, chlorophyⅡ contents, dry weight and soluble proteins among the 3 cultivars.

해녀콩의 발아와 생장시 Canavanine 의 이용과 Canavanase 의 활성에 대하여

권영명(Young Myung Kwon),정흥채(Hung Chae Chung),고석찬(Suck Chan Koh),홍영남(Young Nam Hong) 한국식물학회 1986 Journal of Plant Biology Vol.29 No.2

Canavanine content of the cotyledons of Canavalia lineata decreased gradually during germination and growth of seedlings but continued to increase in roots and leaves. After abscission of cotyledons, canavanine content of leaves depleted competely. The activity of canavanase could be detected in leaves and roots, but not in cotyledons. High arginase activity was observed in the cotyledons of seeds at the early imbibition period. During the growth of seedlings, cotyledonary canavanine appeared to be transported to the growing poprtions of the seedlings where it could be utilized through nitrogen metabolic pathways. In crude cell-free extracts of leaves, maximum activities of canavanase or arginase appeared in 30mM Tris-HCl buffer (pH 9.0) or 30mM NaHCO_3 buffer (pH 10.0), respectively. The activities of these two enzymes differed from each other when treated with Co^2+ or Mn^2+. These results support the idea that canavanase and arginase might be different enzymes.

Alantolactone 의 구조와 생물학적 활성

권영명 (Young Myung Kwon) 한국식물학회 1974 Journal of Plant Biology Vol.17 No.2

To elucidate the relationship between chemical structure and biological activity of alantolactone, and also to investigate the relationship between the growth of cells and the respiration of Chlorella pyrenoidosa affected by alantolactone, alantolactone and isoalantolactone were isolated from Inula helenium L., and di-, and tetrahydroalantolactones were prepared by the hydrogenation. At a concentration of 5×10exp(-5)M alantolactone, the growth rate of Chlorella was greately reduced. The viability of cells was also reduced over 50% within 2 hr at a concentration of 2.5×10exp(-4)M alantolactone. However, oxygen uptake was increased by 20% over 3 hr. And ^(14)CO_2 production from glucose-1-^(l4)C. glucose-6-^(14)C and ^(14)C-acetate-U.L. was also increased by alantolactone. Biological activity of alantolactone was significantly reduced by cysteine, reduced glutathione or cystine but not by tryptophan or histidine. It was detected by spectrophotometrically and by TLC that alantolactone was also reacted with thiols except cystine. The solution of alantolactone reacted with thiol gave the UV absorption spectrum of α-saturated γ-lactone, and most of SH groups were disappeared by the addition reaction. From the reaction mixture of alantolactone and cysteine, a lactone adduct was isolated and purified. Isoalantolactone had shown similar activity as alantolactone, however, it was appeared that di-, and tetrahydroalantolactones were not only inactive biologically but also in vitro. It was concluded that there was no correlationstip between increased respiration rate and mortality of Chlorella. During the respiration TCA cycle was activated, however it was uncertain that the activation of EMP or HMP was also appeared. Alantolactone and isoalantolactone were biologically active compounds but others were inactive. The reactivity of α-methylene γ-lactone moiety toward SH group was principally responsible for its biological activity in sesquiterpene lactones.