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The influences of saponin on the extraction both of D. Stramonium alkaloid and menthol oil have been studied. The results show, as are shown Fig.I and Fig.Ⅱ that extracts of D. Stramonium alkaloid and menthol oil are increased by saponin predicting the possiblities that insoluble components of drug can be solubilized in extraction process by saponin containing in drug.
There are only few papers on the relationship between saponin and enzyme. In a paper by R.J. Rossiter, et al., the effect of saponin on alkaline phosphatase and tributyrin esteraase was reported. According to C.A. Marsh and G.A.M. Ceuty, bean saponin inhibits the glucournidase. This present study describes the effect of saponin on emulsin to amygdalin. It was found that the saponin inhibited the enzymatic action of emulsin to amygdalin as Fig. Ⅰ. represented and precipitate was formed when saponin was added to emulsin solution. The precipitate showed original enzymatic action on amygdalin again by addition into the substrate solution and this enzymatic action was increased by agitation and decreased by addition of saponin as it can be seen in table Ⅱ. It seemed that emulsin-saponin precipitate was dissociated again in greater degree in water medium than in saponin solution. The apparent competitive inhibition of saponin can be also said to be responsible to the similar structural feature of saponin and amygdalin in glycosidal linkage. Graphical representations of Fig.Ⅱ and Fig.Ⅲ however, do not show typical nature of competitive inhibition. Such a complexity presumably due to the change of reaction system from homogenous reaction to heterogeneous one by the precipitation of enzyme.
The position of galloyl groups in acertannin, as 3, 6-digalloylpolygalito, has been established by well-defined processes. In the courses of the processes eight new compounds, octamethoxyacertannin, 2, 4-dimethoxy-1, 5-anhydro-D-sorbitol, 6-tosylpolygalito, 2, 3, 4-tri-benzoyl-6-tosylpolygalitol, 3, 6-anhydro-1, 5-anhydro-D-sorbitol, and 2, 4-ditosyl-3, 6-anhydro-1, 5-anhydro-D-sorbitol, have been characterized.
In the light of quality comparison the ratio of panaxadiol and panaxatriol contents was assayed in the extract of various ginseng products, whose origin of production and species of the original plants are different. Aglycone compositions of other ginsengs were not comparable with Korean ginseng in their ratio of panaxadiol and panaxatriol contents of dammarane glycosides.
As one of projects of this Institute, 330 species of plants which are currently used as herb drugs in Korea were screened for the presence of alkaloids, phenolic compounds, flavonoids, chalcones, lactones, glycosides, carbohydrates, terpenoids, steroids, proteins, polypeptides, saponins, and organic acids. The most reliable presence of alkaloids detected by thin layer chromatography is presented by screening of 52 species.
As the projects of this institute, 280 species of plants which are currently used as herb drugs in Korea were screened on the presence of alkaloids, phenolic compounds, flavonoids, chalcones, lactones, glycosides, carbohydrates, terpenoids, steroids, proteins, polypeptides, saponins, and organic acids, and the most reliable presence of alkaloids detected by thin layer chromatography is added by screening of 75 species.