미류나무절 교잡종 포플러의 생장특성 및 내병성에 의한 선발

구영본(Yeong Bon Koo),김인식(In Sik Kim),여진기(Jin Kie Yeo),최완용(Wan Yong Choi) 한국산림과학회 2002 한국산림과학회지 Vol.91 No.4

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Composite Interval Mapping에 의한 사시나무 전형매 차대의 2년생 생장 형질과 관련된 QTL(Quantitative Trait Loci)의 동정

김용률 ( Yong Yul Kim ),강범용 ( Bum Yong Kang ),최형순 ( Hyung Soon Choi ),홍용표 ( Yong Pyo Hong ),여진기 ( Jin Kie Yeo ),구영본 ( Yeong Bon Koo ) 한국산림과학회 2004 한국산림과학회지 Vol.93 No.3

We studied QTL(quantitative trait loci) influencing 2-year growths such as height increment, diameter at root collar, stem dry weight, root dry weight and total biomass of full-sib progenies between two selection clones, Odae 19 and Bonghyun 4 in Populus davidiana. Using 215 AFLP(amplified fragment length polymorphism) markers, we constructed 16 framework maps covering 545 cM with the average marker density of 12.8cM. CIM(composite interval mapping) showed that two Q_(Pd-Hti)(21.8 cM in map 8) and Q_(Pd-Root)(Pd-Root)(0.01 -4 cM in map 12), were predominantly associated with the traits. Q_(Pd-Hti) significantly accounted for 11.1 % of total phenotypic variation for height increment. Q_(Pd-Root), had a significant pleiotropic effect on diameter at root collar, root dry weight, primary root dry weight and total biomass dry weight explaining 11.6~12.3 % of their phenotypic variations. The two QTLs had no interaction between them, which indicates that they independently control the height increment and the other traits, respectively. Using the single marker analysis, we also identified two independent QTLs, named as QrGM59 and QrGM127, with relatively small effect on height increment, stem dry weight, fine root dry weight and total biomass dry weight. Q,.GM59 was significantly related with height increment explaining 5.3% of phenotypic variation and had no interaction with Q_(rGM59), Q_(rGM127) showed a weak interaction with Q_(Pd-Root) for stem dry weight and total biomass dry weight, and it accounted for 6.2% and 5.4% of the individual phenotypic variation, respectively. The dominant alleles at the four QTLs were inherited from the mother tree, clone Odae 19 which had already showed the best survival rate and good growth at poor planting site in the previous clone stability test. The results in this study indicate that the above- and below-ground growth of P. davidiana could be independently controlled by few genes, and that the QTL markers will be successfully used for selecting clones with good growth in both traits.

Open-Top chamber 내(內)에서 오존에 노출(露出)시킨 현사시 5개(個) 클론의 생장량(生長量)과 오존에 대(對)한 민감성(敏感性)과의 관계(關係)

김태규,이경준,김군보,구영본,Kim, Tae Kyu,Lee, Kyung Joon,Kim, Goon Bo,Koo, Yong Bon 한국산림과학회 2000 한국산림과학회지 Vol.89 No.1

본 연구는 수목의 오존에 대한 피해정도가 수목 고유의 생장속도와 관련되어 있는지를 검증하기 위하여 실시하였다. 생장이 우수한 현사시(Populus alba ${\times}$ P. glandulosa) 4호 품종 중에서 두 개 클론(72-30, 72-16)과 생장이 비교적 저조한 현사시 2호 품종 중에서 3개 클론(72-28, 72-27, 72-19)을 본 실험에서 비교하였다. 위의 5개 클론의 삽수를 4월초 $2{\ell}$ 화분에 삽목하여 증식시킨 후, 8월 하순에 야외에서 3개의 open top chamber(직경 2.5m, 높이 2.0m) 내에서 30일 동안 70ppb와 130ppb의 오존에 1일 5시간씩 노출하였다. 대조구는 활성탄으로 여과하여 오존의 농도가 30ppb이하로 유지되도록 하였으며, 각 클론별, 처리별로 20개의 화분을 반복으로 사용하였다. 대조구에서 두 품종간의 생장비교는 현사시 4호가 현사시 2호보다 잎 건중량에서 73%, 뿌리 건중량에서 64%, 총건중량에서 38% 더 우수하여 현사시 4호의 생장속도가 2호보다 우수함을 입증하였다. 오존에 의한 가시적 피해는 70ppb 오존처리에서는 나타나지 않았으며, 130ppb 처리에서 현사시 4호에서만 관찰되었으며, 성숙잎에서 전형적인 작은 갈색 반점들로 나타났으며, 성숙잎의 조기낙엽을 가져왔다. 70과 130ppb 오존처리는 5개 모든 클론의 수고생장, 잎과 뿌리의 건중량과 총건중량을 감소시켰는데, 이 중에서 뿌리생장을 가장 크게 감소시켰다. 70ppb 오존에서 뿌리의 감소량은 현사시 4호가 39.7%이고, 현사시 2호는 13.8%이었다. 130ppb 오존에서도 현사시 4호의 뿌리감소량은 47.4%이고, 현사시 2호는 34.9%이었다. 이로 인하여 shoot/root율은 130ppb에서 현사시 4호는 63.4% 증가한 반면, 현사시 2호는 22.1% 증가에 그쳤다. 오존에 의한 가공전도도와 순광합성량의 변화에서도 현사시 4호가 현사시 2호보다 더 많이 감소하였다. 특히 130ppb 오존에서 현사시 4호의 순광합성량은 69.5% 감소한 반면에, 현사시 2호는 31.5% 감소하였다. 따라서 위의 여러 가지 생리적 반응을 근거로 하여 생장이 우수한 현사시 4호가 생장이 상대적으로 적은 현사시 2호보다 오존에 더 민감하다고 판단할 수 있으며, 수목의 오존에 대한 피해정도는 수목 고유의 생장속도가 관련되어 있다고 결론 짓는다. This study was conducted to test a hypothesis that sensitivity of trees to ozone exposure was related to their growth rates. Two cultivars of Populus alba ${\times}$ P. glandulosa with different genetic growth potential were used for the comparison. Two clones(72-30, 72-16) of cultivar No. 4 with fast growing potential and three clones(71-28, 72-27, 72-19) of cultivar No. 2 with slow growing potential were propagated in early spring by cutting in $2-{\ell}$ plastic pots. They were grown outdoor for 5 months and exposed in late August for 30 days to 70 and 130ppb ozone in a open-top chambers(2.5m in diameter and 2m in height). Ozone concentration in a control chamber was maintained below 30ppb by filtering with activated charcoal. Each treatment was replicated twenty times. In a control chamber, cultivar No. 4 grew 73%, 64%, and 38% faster than cultivar No. 2 in leaf weight, root weight, and total dry weight, respectively. Visible injury was observed only in cultivar No. 4 in 130ppb treatment. Ozone treatment at both 70 and 130ppb decreased height growth, dry weight of leaf, root, and entire plants in all five clones. Particularly root growth was reduced by 39.7% and 13.8% in cultivar No. 4 and No. 2, respectively, in 70ppb treatment. Consequently, shoot/root ratio of cultivar No.4 was increased by 63.4%, while that of cultivar No.2 was increased by 22.1%. Stomatal conductance decreased more in cultivar No.4 than in cultivar No.2. Net photosynthesis of cultivar No.4 at 130ppb ozone decreased by 69.5%, while that of cultivar No.2 decreased by 31.5%. Above mentioned physiological responses of two cultivars to ozone strongly suggested that fast growing cultivar No.4 was more sensitive to ozone than slow growing cultivar No.2. It was concluded that sensitivity of trees to ozone exposure was closely related to their growth rates.

도심지 은행나무 가로수의 엽록소 함량 및 유전변이 특성

김판기(Pan Gi Kim),구영본(Yeong Bon Koo),이재천(Jae Cheon Lee),배상원(Sang Won Bae),이용섭(Yong Sub Yi),정용문(Yong Moon Cheong) 한국농림기상학회 2001 한국농림기상학회지 Vol.3 No.2

Ginkgo biloba L. has been planted in the city as street trees because reported as resistant species to air pollutant. Especially, the trees planted on the street of `Cheongro`, Mt `Nam`, and `Jamsil` have been exposed to air pollutant for a long time. This study was conducted to examine chlorophyll contents and genetic variation of Ginkgo biloba in the areas. Chlorophyll contents measured in the above three areas were variable although the the diameter at breast height measured in `Cheongro` and Mt. `Nam` were constant. In addition, the result showed positive relation between chlorophyll contents and DBH in this study. Eight enzyme systems were analyzed in megagametophytes which were collected in the areas and separated to two groups based on chlorophyll contents. All the enzymes appeared to be polymorphic : Got-2, Pgi-2, Pgm, Acon, Mnr, Mdh, Skdh, and 6Pgd. The sensitive (S) groups varied from 1.253 to 2.571 in the genetic diversity and the tolerant (T) groups ranged from 1.416 to 2.825. The observed single locus heterozygosities (H_o) ranged from 0.056 to 0.611 in the S groups, and from 0.179 to 1.643 in the T groups. The expected heterozygosities (H_e) ranged from 0.208 to 0.629 in the S groups and from 0.321 to 0.658 in the T groups. In addition, the H_o values averaged over all loci were 0.326 for the T groups and 0.299 for the S group, respectively. A difference between the two groups was 0.027. The T groups had the unique alleles and genotypes and all the parameters for genetic diversity showed that the T groups had higher genetic diversity than the S groups.

도심지역 은행나무 가로수의 엽록소 함량 및 유전변이

김판기(Pan Gi Kim),이용섭(Yong Sub Yi),구영본(Yeong Bon Koo),이재천(Jae Cheon Lee),정용문(Yong Moon Cheong) 한국농림기상학회 2001 학술발표논문집 Vol.- No.-

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I-SSR 분석에 의한 노각나무 천연집단의 유전변이

양병훈(Byeung-Hoon Yang),한상돈(Sang-Don Han),구영본(Yeong-Bon Koo),박용구(Yong-Goo Park) 한국자원식물학회 2006 한국자원식물학회지 Vol.19 No.1

본 연구는 우리나라 특산수종이며 조경 및 원예적 가치가 높은 노각나무 유전변이를 조사하기 위해 6개 천연집단을 선발하여 DNA I-SSR 표지자를 사용, 유전다양성 및 유전구조를 조사하였다. 5개의 I-SSR primer(#813, 815, 818, 820, 823)에서 총 61개의 증폭산물을 관찰할 수 있었으며, 유전 다양성을 나타내는 P(Percentage of polymorphic loci)값과S.I.(Shannon s information Index)가 남쪽에 분포하는 오봉산(P=88.5%, S.I.=0.467), 금산(P=86.9%, S.I.=0.427), 바랑산(P=83.6%, S.I.=0.425)집단이 높았으며, 북쪽(내륙)에 분포하는 소백산(P=80.3%, S.I.=0.396), 지리산(P=77.1%, S.I.=0.368), 가야산(P=75.4%, S.I.=0.358)집단은 낮았다. 전체 유전변이 중 11.8%만이 집단간에 기인하는 것으로 나타났고, 나머지 88.2%는 집단내 개체간의 차이에서 기인하였다. 유전거리를 이용하여 UPGMA법에 의한 유집분석을 실시한 결과 지리적 분포에 대한 뚜렷한 경향은 나타나지 않았다. We investigated the genetic variation in Stewartia koreana Nakai by examining 61 I-SSR amplicons in 120 individuals distributed among six natural populations in Korea. The overall percentage of polymorphic I-SSR amplicons was 81.9% and mean number of amplicons per I-SSR primer was 12.2. Levels of genetic diversity within 6 populations were similar each other[Shannon s Index 0.358~0.467(mean: 0.407)]. The Mt. Obong population had the highest level of genetic diversity and was most distinctive from the other populations. Most variation existed among individuals within population(88.2%). Genetic differentiation among populations(ΦST) was 0.118. The UPGMA dendrogram based on the genetic distance failed in showing decisive geographic relationships.

Open - Top chamber 내에서 오존에서 노출시킨 현사시 5 개 클론의 생장량과 오존에 대한 민감성과의 관계

김태규(Tae Kyu Kim),이경준(Kyung Joon Lee),김군보(Goon Bo Kim),구영본(Yong Bon Koo) 한국산림과학회 2000 한국산림과학회지 Vol.89 No.1

This study was conducted to test a hypothesis that sensitivity of trees to ozone exposure was related to their growth rates. Two cultivars of Populus alba × P. glandulosa with different genetic growth potential were used for the comparison. Two clones(72-30, 72-16) of cultivar No. 4 with fast growing potential and three clones(71-28, 72-27, 72-19) of cultivar No. 2 with slow growing potential were propagated in early spring by cutting in 2-ℓ plastic pots. They were grown outdoor for 5 months and exposed in late August for 30 days to 70 and 130ppb ozone in a open-top chambers(2.5m in diameter and 2m in height). Ozone concentration in a control chamber was maintained below 30ppb by filtering with activated charcoal. Each treatment was replicated twenty times. In a control chamber, cultivar No. 4 grew 73%, 64%, and 38% faster than cultivar No. 2 in leaf weight, root weight, and total dry weight, respectively. Visible injury was observed only in cultivar No. 4 in 130ppb treatment. Ozone treatment at both 70 and 130ppb decreased height growth, dry weight of leaf, root, and entire plants in all five clones. Particularly root growth was reduced by 39.7% and 13.8% in cultivar No. 4 and No. 2, respectively, in 70ppb treatment. Consequently, shoot/root ratio of cultivar No.4 was increased by 63.4%, while that of cultivar No.2 was increased by 22.1%. Stomatal conductance decreased more in cultivar No.4 than in cultivar No.2. Net photosynthesis of cultivar No.4 at 130ppb ozone decreased by 69.5%, while that of cultivar No.2 decreased by 31.5%. Above mentioned physiological responses of two cultivars to ozone strongly suggested that fast growing cultivar No.4 was more sensitive to ozone than slow growing cultivar No.2. It was concluded that sensitivity of trees to ozone exposure was closely related to their growth rates.