I-SSR 분석에 의한 노각나무 천연집단의 유전변이

양병훈(Byeung-Hoon Yang),한상돈(Sang-Don Han),구영본(Yeong-Bon Koo),박용구(Yong-Goo Park) 한국자원식물학회 2006 한국자원식물학회지 Vol.19 No.1

본 연구는 우리나라 특산수종이며 조경 및 원예적 가치가 높은 노각나무 유전변이를 조사하기 위해 6개 천연집단을 선발하여 DNA I-SSR 표지자를 사용, 유전다양성 및 유전구조를 조사하였다. 5개의 I-SSR primer(#813, 815, 818, 820, 823)에서 총 61개의 증폭산물을 관찰할 수 있었으며, 유전 다양성을 나타내는 P(Percentage of polymorphic loci)값과S.I.(Shannon s information Index)가 남쪽에 분포하는 오봉산(P=88.5%, S.I.=0.467), 금산(P=86.9%, S.I.=0.427), 바랑산(P=83.6%, S.I.=0.425)집단이 높았으며, 북쪽(내륙)에 분포하는 소백산(P=80.3%, S.I.=0.396), 지리산(P=77.1%, S.I.=0.368), 가야산(P=75.4%, S.I.=0.358)집단은 낮았다. 전체 유전변이 중 11.8%만이 집단간에 기인하는 것으로 나타났고, 나머지 88.2%는 집단내 개체간의 차이에서 기인하였다. 유전거리를 이용하여 UPGMA법에 의한 유집분석을 실시한 결과 지리적 분포에 대한 뚜렷한 경향은 나타나지 않았다. We investigated the genetic variation in Stewartia koreana Nakai by examining 61 I-SSR amplicons in 120 individuals distributed among six natural populations in Korea. The overall percentage of polymorphic I-SSR amplicons was 81.9% and mean number of amplicons per I-SSR primer was 12.2. Levels of genetic diversity within 6 populations were similar each other[Shannon s Index 0.358~0.467(mean: 0.407)]. The Mt. Obong population had the highest level of genetic diversity and was most distinctive from the other populations. Most variation existed among individuals within population(88.2%). Genetic differentiation among populations(ΦST) was 0.118. The UPGMA dendrogram based on the genetic distance failed in showing decisive geographic relationships.

희귀(稀貴) 수종(樹種) 눈향나무 집단(集團)의 동위효소(同位酵素) 분석(分析)에 의한 유전변이(遺傳變異) 연구(硏究)

한상돈(Sang-Don Han),양병훈(Byeung-Hoon Yang),권해연(Hae-Youn Kwon) 한국자원식물학회 2006 한국자원식물학회지 Vol.19 No.1

우리나라 고산 지역에 제한적으로 자생하는 희귀 유전자원인 눈향나무(Juniperus chinensis var. sargentii HENRY)의 설악산 및 한라산 집단을 대상으로 동위효소 분석에 의한 유전적 다양성을 조사하였다. 총 7개 동위효소에서 11개의 재현성 있는 유전자좌가 분석되었으며, 이중 Mdh-1, Mdh-2, Mdh-3 및 Pig-1 유전자좌를 제외한 7개 유전자좌에서 다형성이 관찰되었다. 분석된 두 집단의 유전변이량은 각각 A=2.2, Ae=1.61, P95=54.5, Ho=0.179, He=0.287(설악산 집단)과 A=2.1, Ae=1.48, P95=63.6, Ho=0.270, He=0.250(한라산 집단)으로 국내 타 침엽수종으로부터 동위효소 분석을 통해 추정된 유전변이량에 비해 다소 높은 경향을 보였으며, 분석 집단간 유전적 분화 정도는 그리 높지 않은 것으로 나타났다(F_ST=0.039). 설악산 집단의 경우 이형접합도의 관찰치가 기대치에 비해 매우 낮았으며 근교계수 값이 매우 높게 나타나(F=0.376), 전반적으로 근친교배 또는 유전적 부동의 영향을 많이 받고 있는 것으로 추정되었다. 이는 설악산 눈향나무 집단의 분포 면적이나 개체수가 한라산 집단에 비해 매우 적기 때문인 것으로 추정되며, 향후 설악산 집단의 보존을 위한 보다 적극적인 노력이 필요한 것으로 사료된다. Genetic variation of two Juniperus chinensis var. sargentii populations in Mt. Seorak and Mt. Halla was investigated by isozyme analysis at reproducible 11 loci of 7 isozyme systems(Aat-1, Aat-2, Gdh, Idh, Lap, Mdh-1, Mdh-2, Mdh-3, 6Pgd, Pgi-1, and Pgi-2), of which 7 loci were polymorphic. The levels of genetic diversity of two populations were A=2.2, Ae=1.61, P95=54.5, Ho=0.179, He=0.287(Mt. Seorak population) and A=2.1, Ae=1.48, P95=63.6, Ho=0.270, He=0.250(Mt. Halla population), respectively. These values were similar to and/or somewhat higher than those observed in other Korean native conifers. Moderately low degree of genetic differentiation was observed between 2 analyzed populations (F_ST=0.039). Heterozygosity of the population in Mt. Seorak was significantly lower than expected, and much high level of inbreeding coefficient(F=0.376) was observed. Considering the limited population size and distribution range of the population, the population seemed to be influenced by inbreeding and/or random genetic drift, Consequently, Mt. Seorak population should be considered to be a more important candidate for the conservation of J. chinensis var. sargentii.

설악산 격리 잔존 눈잣나무 집단의 유전적 성상

홍용표 ( Yong Pyo Hong ),권해연 ( Hae Yun Kwon ),양병훈 ( Byeung Hoon Yang ),이석우 ( Seok Woo Lee ),김찬수 ( Chan Soo Kim ),한상돈 ( Sang Don Han ) 한국산림과학회 2004 한국산림과학회지 Vol.93 No.5

Genetic variation of a dwarf stone pine(Pinus pumila Regel) population in Mt. Seorak, which is an unique and the southern peripheral population in South Korea, was investigated using allozyme markers at 15 loci in 50 individuals. The estimates of genetic variation were A=1.9, P_(95)=46.7%, H_o=0.138, and H_e=0.181. These values were somewhat different from those of other Korean native Pin us species, and much lower than those value analysed in Russian and Japanese populations of the same species. Of the nine polymorphic loci studied, a significant heterozygote deficiency was observed in three loci, AAT-2, GDH and MNR(p<0.05), and a relatively high level of inbreeding coefficient (F=0.205) was estimated within the study population. Possible explanations for these observations may be as follows; (1) several genetic patches might exist within the study population and inbreeding might be going on among genetically close individuals, (2) Wahlund effect might be resulted from sampling individuals from several sexually isolated genetic patches. Considering not only the relatively low level of genetic diversity, small population size, and geographic isolation from other populations, but man and/or wildlife-caused disturbances, it is necessary to provide suitable conservation methods for the study population immediately.

우리나라 野生 차나무(Camellia sinensis L.) 集團의 種子特性 變異

송정호(Jeong-Ho Song),장경환(Kyung-Hwan Jang),임효인(Hyo-In Lim),양병훈(Byeung-Hoon Yang),김준혁(Joon-Hyeok Kim) 한국차학회 2011 한국차학회지 Vol.17 No.2

This study was conducted to investigate the variation of seed characteristics among populations and among clumps within population of wild tea (Camellia sinensis L.) distributed in Korea. Fruits and seeds were collected from 225 clumps in 13 populations and their seven seed characteristics were analyzed using multivariate analysis. The mean values of seed characteristics were 12.4 mm in seed length, 13.9 mm in seed width, 0.90 in seed length/width, 13.1 mm in seed thickness, 0.95 in seed length/thickness, 1.07 in seed width/thickness, and 0.95 g in seed weight, respectively. Coefficients of variation were low (5.6-9.3%) in all seed characteristics except seed weight (27.7%). ANOVA analysis showed significant differences among populations and among clumps within population in all seed characteristics. According to Nested analysis, variance contributions among populations in the characters excluding seed length/thickness and seed weight were higher than those among clumps within population. Cluster analysis using average linkage method showed three groups to Euclidean distance 0.45. But, there was no significant relationship between population association and geographical distribution. The results of principal component analysis for seven seed characteristics showed that primary two principal components (PC's) explained 90.8% of the total variation. The first PC accounted for 75.0% of the variability which correlated with seed length, seed width and seed thickness, and the second PC accounted for 15.8% of the variability which correlated with seed weight. According to correlation analysis, the populations which located in high latitude and low altitude place have more heavy seed than low latitude and high altitude populations.

산딸나무(Cornus kousa) 풍매차대(風媒次代)의 발아, 생장 및 엽형(葉型) 변이

송정호 ( Jeong Ho Song ),구영본 ( Young Bon Goo ),한심희 ( Sim Hee Han ),양병훈 ( Byeung Hoon Yang ),박형순 ( Hyung Soon Park ) 한국산림과학회 2006 한국산림과학회지 Vol.95 No.3

들쭉나무 격리잔존 2개 집단의 유전변이

한상돈 ( Sang Don Han ),홍용표 ( Yong Pyo Hong ),권해연 ( Hae Yun Kwon ),양병훈 ( Byeung Hoon Yang ),김찬수 ( Chan Soo Kim ) 한국산림과학회 2005 한국산림과학회지 Vol.94 No.4

기내배양에 의한 차나무 대량증식기술개발

손윤자(Yun-Ja Son),임창숙(Chang-Suk Lim),양병훈(Byeung-Hoon Yang),박용구(Young-Goo Park) 한국차학회 2007 한국차학회지 Vol.13 No.1

재래종 차나무 종자를 무균상태에서 발아시켜 생장한 차나무의 액아가 있는 작은 줄기를 재료로 대량증식 방법을 실험하였다. 6가지 배지 중, MS와 WPM 배지에서 좋은 줄기 생장을 나타냈다. 대량증식 실험은 MS, WPM 배지에 BA, 2iP 및 TDZ를 6단계별로 처리하여 배양한 결과 BA 5 μM를 첨가한 MS배지와WPM배지에서 좋은 생장을 나타내어 MS배지와 WPM 배지에 BA 5 μM을 고정시키고 GA3를 농도별로 처리한 결과 GA3 5 μM을 첨가한 WPM배지에서 64개의 가장 많은 수의 줄기를 얻었다. 차나무의 발근실험에서는 IBA 30 μM를 첨가한 MS배지에서 뿌리 발생률이 높았으며 줄기생장도 좋았으나 그 이상의 농도에서는 뿌리 생장에 비해 줄기 성장이 좋지 않았다. 뿌리가 발생한 줄기를 인공토양에 이식하였으며 이식한 3~4주후부터 뿌리 활착이 양호하게 나타났다. 같은 모수에서 대량 증식된 개체간의 유전자 변이가 발생하는 지를 밝히기 위해 PCR 실험을 실시하였다. 그 결과 전체 20개의 primer에 대해 같은 모수에서 발생한 개체 간에는 유전적 변이가 나타나지 않았다. 이러한 결과로 볼 때 유전적으로 동일한 영양체의 대량생산을 위하여 조직배양법은 매우 적절한 방법임을 밝혀냈다. An optimal protocol for mass propagation of Korean tea plants was studied by tissue culture methods. Korean tea seedlings which were cultured in vitro were used for mass propagation. In order to induce the germination and growth of the tea plants, 6 different media were used. Out of 6 levels of BA, 2iP and TDZ with the media, MS and WPM with 5 μM BA showed the best result among them. A number of multishoots were propagated in the MS and WPM with BA and GA3, WPM with 5 μM BA and 5 μM GA3 was represented much number of shoots as 64 shoots. The multishoots rooted with high frequency in MS with IBA 30 μM, but the shoot growth began to decrease in the media with more than concentration of IBA 30 μM. The rooting shoots were planted in the culture*Corresponding author(E-mail: ygpark@knu.ac.kr, Tel: 053-950-5747, Fax:053-950-6708) soil (perlite : vermiculite=1 : 1 ) and the growth and rooting of the plants were proved to be good result after 3 or 4 weeks from planted. In order to identify genetic variation, we observed PCR band of the plants. The PCR band patterns were not difference within individuals from mass propagated shoots of same mother plants. This method appears to be an optimal technique for mass propagating shoots of the tea plants.

희귀식물(稀貴植物) 눈측백나무(Thuja koraiensis Nak.)의 삽목증식(揷木增殖)

송정호 ( Jeong Ho Song ),이정주 ( Jung Joo Lee ),구영본 ( Yeong Bon Koo ),이갑연 ( Kab Yeon Lee ),한상돈 ( Sang Don Han ),양병훈 ( Byeung Hoon Yang ) 한국산림과학회 2006 한국산림과학회지 Vol.95 No.4