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뽕잎 추출물 기원 Flavonoid Rich Fraction의 항비만효과
고은지(Eun Ji Go),류병렬(Byung Ryeol Ryu),양수진(Su Jin Yang),백종섭(Jong Suep Baek),유수지(Su Ji Ryu),김현복(Hyun Bok Kim),임정대(Jung Dae Lim) 한국약용작물학회 2020 한국약용작물학회지 Vol.28 No.6
Background: This study investigated the anti-obesity effect of the flavonoid rich fraction (FRF) and its constituent, rutin obtained from the leaf of Morus alba L., on the lipid accumulation mechanism in 3T3-L1 adipocyte and C57BL/6 mouse models. Methods and Results: In Oil Red O staining, FRF (1,000 ㎍/㎖) treatments showed inhibition rate of 35.39% in lipid accumulation compared to that in the control. AdipoRedTM assay indicated that the triglyceride content in 3T3-L1 adipocytes treated with FRF (1,000 ㎍/㎖) was reduced to 23.22%, and free glycerol content was increased to 106.04% that of the control. FRF and its major constituent, rutin affected mRNA gene expression. Rutin contributed to the inhibition of Sterol regulatory element binding protein-1c (SREBP-1c) gene expression, and inhibited the transcription factors SREBP-1c, peroxisome proliferator-activated receptor gamma (PPAR-γ), CCAAT/enhancer binding protein α (C/EBPα), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC). In addition, the effect of FRF administration on obesity development in C57BL/6 mice fed high-fat diet (HFD) was investigated. FRF suppressed weight gain, and reduced liver triglyceride and leptin secretion. FRF exerted potential anti-inflammatory effects by improving insulin resistance and adiponectin levels, and could thus be used to help counteract obesity. The mRNA expressions of PPAR-γ, FAS, ACC, and CPT-1 were determined in liver tissue. Quantitative real-time PCR analysis was also performed to evaluate the expression of IL-1β, IL-6, and TNF-α in epididymal adipose tissue. Compared to the control group, mice fed the HFD showed the up-regulation in PPAR-γ, FAS, IL-6, and TNF-α genes, and down-regulation in CPT1 gene expression. FRF treatement markedly reduced the expression of PPAR-γ, FAS, IL-6, and TNF-α compared to those in HFD control, whereas increased the expression level of CPT1. Conclusions: These results suggest that the FRF and its major active constituent, rutin, can be used as effective anti-obesity agents.
Breeding and Evaluation of Cultivation Adaptability of New Medicinal Hemp Clone ‘Pink pepper’
Byeong Ryeol Ryu(류병렬),Eun Ji Go(고은지),Gyeong Ju Gim(김경주),Ye Rim Shin(신예림),Md Jahirul Islam,Md Obyedul Kalam Azad,Md Hafizur Rahman,Md Rana Soyel,Tae Hyung Kwon(권태형),Chang Hyuck Kim(김창혁),Joon Hee Han(한준 한국약용작물학회 2022 한국약용작물학회 학술대회논문집 Vol.2022 No.1