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제주산 우뭇가사리(Gelidium amansii )로부터 DMSO 추출과 EDTA 수세법에 의한 상용화 아가로스 제조
강태환 ( Tai Hwan Kang ),이승홍 ( Seung Hong Lee ),백종석 ( Jong Seok Baik ),강병식 ( Byung Sik Kang ),이정석 ( Jung Suck Lee ),이남호 ( Nam Ho Lee ),전유진 ( You Jin Jeon ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.6
Agar was prepared from Gelidium amansii collected from Jeju Island, South Korea. This agar preparation has high gel strength and low sulfate content compared with G. amansii agar from Morocco. Accordingly, agarose was made from the Jeju agar through the consecutive refining processes of dimethyl sulfoxide (DMSO) extraction and ethylene diamine tetra acetic acid (EDTA) washing. The physicochemical properties of the resulting agarose were compared with those from agarose prepared using only DMSO extraction. Consecutive DMSO extraction and EDTA washing more strongly affected the physicochemical properties of the agarose (purified agarose) compared with the use of DMSO extraction alone. These properties were similar to those of commercial agarose used for electrophoresis. In DNA electrophoresis, the separation and movement speed of the purified agarose were similar to those of the commercial agarose. In a 13C NMR analysis, the purified agarose exhibited the same carbon peak as the commercial agarose. When observed under scanning electron microscopy, the agar had an even and smooth surface without irregularities or pores, and the purified agarose had a wide surface area with a large number of pores; the commercial agarose had an irregular surface that would allow the solvent to easily permeate. These results illustrate that the physicochemical properties of agarose prepared from DMSO extraction and EDTA washing were more effective than those observed after DMSO extraction alone; thus, these processes used in succession will be useful in agarose industries.
임분내 광환경의 차이에 따른 주요 참나무 수종의 생장과 엽록소 함량 변화에 관한 연구
권기원(Ki-Won Kwon),최정호(Jeong-Ho Choi),송호경(Ho-Kyung Song),강병식(Byung-Sik Kang) 한국산림바이오에너지학회 2003 산림바이오에너지 Vol.22 No.3
참나무류 주요 수종을 대상으로 다양한 광선 조건에 식재된 실생묘의 활착 및 상대생장량과 광선에 대하여 민감하게 작용하는 엽록소 함량의 계절적인 변화를 조사하기 위하여 상수리나무 1-1묘, 1-0묘, 신갈나무 1-0묘, 졸참나무 1-0묘, 굴참나무 1-0묘를 상수리나무 임분내에 상층임분 밀도를 자연광의 81%, 34%, 21% 등 3가지로 조절하였다.<br/> 조사대상 수종의 묘목을 20m x 10m 크기의 시험구에 100본씩 식재하여 활착율, 상대생장량, 엽록소 함량을 비교하였다.<br/> 상수리나무 1-1묘가 평균 73%로 다른 수종에 비해 높은 활착율을 보였고, 상수리나무 1-0묘가 66%, 신갈나무와 졸참나무가 각각 56%, 45%였으며, 굴참나무는 41%로 이식후 활착율이 가장 낮게 나타났다. 또한 자연광의 81% 실험구에서 대부분의 주요 참나무류 수고, 근원경 생장이 가장 좋은 경향을 보여 주고 있었으며 자연광의 21% 실험구에서는 오히려 광도가 낮아지면서 생장이 상대적으로 저해되는 경향을 보여 주고 있다. 엽록소 함령(a, b, 총엽록소 함향)은 광도가 낮아지면서 엽록소 함량도 낮아지는 것을 알 수 있었다. 그러나 주요 참나무류 수종의 피음 효과에 대한 생장반응은 지속적으로 관찰해야 하며, 엽록소 함량과 생장에 밀접한 관련이 있는 생리적 특성에 대한 연구도 필요할 것으로 생각된다. This study was subjected to compare seasonal changes of survival rate, relative growth rate, and chlorophyll contents of major oak tree species including Quercus acctissima, Quercus mongolica, Quercus serrata, Quercus variabilis seedlings grown in Quercus acctissima forest under different light intensities. Three light intensities were 81% of sunlight, 34% of sunlight and 21% of sunlight. In each treatment, 100 tree seedlings were planted and survival rate, growth rate, and chlorophyll contents were measured. <br/> The highest survival rate was Quercus acctissima in 73% seedlings compared with those subjected to the other tree seedlings in 45~66%. Lowest survival rate was Quercus variabilis seedlings in 41%. Oaks tree species of the height, the root collar diameters of the relative growth were better in the seedlings grown in 81% light intensities of full sun. But growth rates decreased rapidly in the shade treatment of 21% light intensities of full sun. Lowest chlorophyll contents(chlorophyll a, b and total) were shown at 21% light intensities of full sun, lowest light intensity treatment in this study. This result is thought growth and chlorophyll contents associated with light intensity. Also, Physiological characteristics has to be investigated in near future because photosynthesis and chlorophyll contents were strongly related to tree growth with long periods.