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치과용 레이저 조사가 배양 치수 섬유모세포에 미치는 영향에 관한 연구
정혜전,민병순 大韓齒科保存學會 1997 Restorative Dentistry & Endodontics Vol.22 No.2
The responses of human pulp fibroblastic cells to Ga-As Semi-Conductor-Dens-Bio Laser(Frequency: 5Hz~10,000Hz Model: SD-101A RCA, U.S.A.)) were examined in vitro using pulp fibroblastic cells obtained from the pulp tissue of human tooth. The mitogenic effect of soft laser was assessed by measuring the MTT assay. The morphologic effect for soft laser showed under the scanning and transmission electron microscopy. The results as follows; 1. The mitogenic response of the soft laser was not observed until 4th time of radiation, while the mitogenic response at 4th time increased mitogenic effect by as much as 1.7 fold compared to the control value. 2. The mitogenic response of the soft laser on pulp fibroblast differ from the mitogenic response on other fibroblasts. 3. In scanning electron microscopic study, The microvilli of cell surface increased gradually with width and length after laser radiation, it demonstrate that development of microvilli have close connection with differentiation of cells. 4. Under the transmission electron microscope, The laser-treated cells maintained their elongated shape and a high degree of cellular polarization. The large cell body containing a well developed Golgi complex, a large number of profiles of rough endoplasmic reticulum, and great numbers of mitochondria. 5. The laser-treated cells maintained the long straight bundles of closely apposed microfilaments of individual filaments forming a cross-linked network. These findings suggest that the laser may have important roles in promotion of pulp healing and consequently may be useful for clinical application in pulp regenerative procedures.
아말감과 심미성 수복재료와의 전단 결합강도에 관한 연구
정혜전,민병순 大韓齒科保存學會 1995 Restorative Dentistry & Endodontics Vol.20 No.1
AbstractComposite resin and glass-ionomer cement can be used for the purpose of repair of defective amalgam restoration.The purpose of this study was to evaluate of shear bond strength of esthetic restorative materials to dental amalgam.The materials used in this study were Photo Clearfil BrightOight curing composite resin), Clearfil F II (chemical curing composite resin), Fuji II LCOight curing glass-ionomer ce- ment), Fuji II (chemical curing glass-ionomer cement), All-Bond 2 (intermediary), and Scot- chbond Multi-Purpose (intermediary). A total of 120 acrylic cylinders with amalgam were divided into 8 groups After amalgam condensation, all specimens were stored for 48 hours in water at 37? and tested with Instron universal testing machine between amalgam and composite resins and glass-ionomer cements.The data were analyzes statiscally by ANOVA and Duncan test.The following results obtained ; 1. The shear bond strength of bonded composite resin to amalgam was higher than bonded glass-ionomer cement(P<.OOl). 2. The group 4 had highest shear bond strength with 15.45kgf/cm2 and the group 5 had lowest shear bond strenght with S^ekgi/cmPOOl). 3. In the group 3, 4, 5, 6, the group 3, 4 with All-Bond 2 had higher shear bond strength than the group 5, 6 with Scotchbond MP both in light-curing and in chemical curing. 4. Both in composite resin and glass-ionomer cement, chemical curing materials had higher shear bond stength than light curing materials(P<.OOl).