Sayo Yotsukura , The Articles in English

김한곤 ( Han Kon Kim ) 한국영어영문학회 1972 영어 영문학 Vol.41 No.-

意味論의 對象과 方法

金韓坤(Han-Kon Kim) 서울대학교 언어교육원 1969 語學硏究 Vol.5 No.2

音響音聲學的 方法에 依한 言語合成

金韓坤(Han-Kon Kim) 서울대학교 언어교육원 1968 語學硏究 Vol.4 No.1

한국의 노동력전환에 관한 인구학적 분석: 1960-1990

김한곤(Han-Kon Kim) 영남대학교 인문과학연구소 1994 人文硏究 Vol.16 No.1

Outline of Stratificational Grammar by sydney Lamb

김한곤 ( Han Kon Kim ) 한국영어영문학회 1967 영어 영문학 Vol.24 No.-

勞動의 分化에 關한 生態學的 分析

金漢坤(Kim Han Kon) 한국인구학회 1988 한국인구학 Vol.11 No.2

This study is a replication of Clemente and Sturgis' study conducted among 600 American cities in 1972. The impact of population size, physical density, social density, and age of 56 Korean cities upon the extent of their industrial diversification was analyzed. Hypotheses concerning each independent variable were generated from the theory of the development of the division of labor proposed by Durkheim and subsequently adopted by human ecologists. Zero-order correlation analysis and regression analysis were employed to test the hypotheses. The standardized partial regression coefficients indicated that social density and age of cities exert significant positive impact upon industrial diversification. On the other hand, population size showed a significant negative impact upon industrial diversification. In addition, it was discovered that the combined influence of the independent variables account for 34.6 percent of the variation in industrial diversification. The findings of this study were compared with the results of Clemente and Sturgis' study. Some suggestions for future research are presented and discussed.

Bilingual dictionary 편찬에 대한 소고 (小考)

김한곤 ( Han Kon Kim ) 한국영어영문학회 1970 영어 영문학 Vol.36 No.-

THP-1 세포주를 이용한 화장품 원료의 피부 감작성 대체법 활용 연구

김서영 ( Seo Young Kim ),안수선 ( Su Sun An ),김한곤 ( Han Kon Kim ),이태룡 ( Tae Ryong Lee ) 한국동물실험대체법학회 2009 동물실험대체법학회지 Vol.3 No.1

Evaluation of skin sensitization potential is a major part of safety assessment of new ingredients in cosmetics and drugs to be applied topically. Several studies have shown that contact sensitizers induce co-stimulatory molecules on THP-l cells and have suggested that the expression of these co-stimulatory molecules can be used as a predictive in vitro assay system for contact sensitization. Most cosmetic ingredients are non- or slight- or at most moderate sensitizers. In this study, we evaluated the utility of the expression of co-stimulatory molecules on THP-l cells as a predictive in vitro assay system for discrimination of these slight differences in sensitizing potential of cosmetic ingredients. We investigated the expression of CD54 and/or CD86 on the THP-l cells using flow cytometry after a 24 h exposure to cosmetic ingredients such as resveratrol, polydatin (a derivative of resveratrol), and decursin with two different purities (70% and 98%). The resveratrol showed a mild skin sensitization potential and cell cytotoxicity but the polydatin did not induce any up-regulation of these two markers and did not show cell cytotoxicity at the same concentration with resveratrol. Although both decursins with 70% and 98% purities were classified as mild sensitizers, there was also a definite trend that the decursin with 98% purity was less potent sensitizer than the decursin with 70% purity. These data suggested the possibility of using expression of CD54 and/or CD86 in THP-l cell line as a predictive tool evaluating subtle differences in skin sensitization potential of cosmetic ingredients.

투명감 측정을 통한 제형의 미백 효능 평가와 투명감에 관여하는 요소들에 대한 분석

정춘복 ( Choon Bok Jeong ),김한곤 ( Han-kon Kim ),남개원 ( Gae Won Nam ) 대한화장품학회 2010 대한화장품학회지 Vol.36 No.4

현재까지 피부 미백에 대한 평가는 피부 반사광 전체에 대한 분석이 주를 이루어 왔다. 그 예로 SHV (Saturation, Hue, Value), L* a* b* (CIELAB color space system) 등 색차계를 사용하여 검출된 빛을 몇 가지 기준으로 나누어 분석하는 방법이 이용되어왔으며, 밝기나 채도 변화를 기준으로 피부 미백을 평가해왔다. 하지만 이러한 기존의 미백평가법들은 빛의 전체 피부 반사량을 통한 측정이라는 한계를 가지고 있어 투명감, 화사함, 윤기 등 소비자들이 원하는 새로운 미백 효능의 평가에는 사용하기 힘든 단점이 있었다. 이번 연구에서는 이전까지 정량적으로 표현하기 힘들었던 투명감에 대한 새로운 측정법을 활용하여 8주 동안 자사의 2가지 미백 제형 효능 평가에 사용하였으며, 이를 위해 루미스캔(LumiscanTM)이라고 명명된 편광을 활용한 각도별 반사광 측정 장치를 제작하여 실험에 활용하였다. 또한, 투명감측정 이외에도 15가지 측정치에 대한 실험을 병행하여 투명감에 주요한 영향을 미치는 요소를 분석하고자 하였으며 결과적으로 자사의 2가지 미백 제형은 각각 4, 8주에 5 ~ 9 %의 투명도 개선을 보여주었다. 그리고 피부결(-28 %), 멜라닌(-17 %), 홍반(-7 %), 수분량(15 %), 피부 밝기(6 %) 등이 피부 투명감에 주요한 영향을 주는 요소라는 사실을 보여주었다. Until now, evaluating the efficacy of brightening mainly depends on total reflective light measurement. For example, SHV (Saturation, Hue, Value), L* a* b* (CIELAB color space system) color space system was used and lightness and saturation changes were chosen as major parameters for evaluating brightening effect. However, those parameters were calculated from total reflective light on the skin and it is hard to evaluate perceptive efficacy such as luminescence, and glossy. In this research, we applied new method for estimating change of luminescence of skin by using 'Lumiscan' which uses polarized light for detecting surface and inside reflective light independently. We also tested 15 different parameters for finding correlations between luminescence and those parameters. As a results, our 2 different brightening products showed 5 ~ 9 % increase of luminescence at 4 and 8 weeks. And we also found that skin roughness (-28 %), melanin index (-17 %), redness (-7 %), hydration (15 %), and lightness (6 %) were related to luminescence of skin.

장양(藏釀) 기술이 사용된 전통 고숙성 된장에서 유래한 o-Dihydroxyisoflavone 유도체의 생리활성

김동현 ( Dong Hyun Kim ),박준성 ( Jun Seong Park ),박녹현 ( Nok Hyun Park ),문은정 ( Eun Jeong Moon ),유선혜 ( Sun Hye Yu ),김덕희 ( Duck Hee Kim ),김한곤 ( Han Kon Kim ) 대한화장품학회 2009 대한화장품학회지 Vol.35 No.3

된장은 한국의 독특한 발효 식품이며 장양이라 불리는 전통적인 제조 방법에 의해 제조된다. 이에 본 연구에서는 고숙성 된장에서 새롭게 생성된 성분과 그것의 생리활성을 측정하였다. 5년 숙성된 된장으로부터 새로운 o-dihydroxyisoflavone인 7,3',4'-trihydroxyisoflavone과 기존에 알려진 두 가지의 o-dihydroxyisoflavone을 각각 분리하여 다른 isoflavone과의 항산화 효과 및 미백효과를 비교 측정하였다. Diphenyl-1-picrylhydrazyl (DPPH) 활성 저해 효과는 7,8,4'-trihydroxyisoflavone (compound 1), 7,3',4'-trihydroxyisoflavone (compound 2) 그리고 6,7,4'-trihydroxyisoflavone (compopund 3)은 각각 21.5 ± 0.2, 28.7 ± 0.4 그리고 32.6 ± 0.6 (IC₅₀)을 나타낸 반면 daidzein은 이들에 비해 약한 DPPH 활성 저해 효과를 나타냈다. Superoxide 소거 효과는 L-ascorbic acid에 비해 compound 1 (IC₅₀ = 18.10 ± 0.2 μM)과 2 (IC₅₀ = 10.54 ± 0.4 μM)가 보다 효과적인 반면 compound 3과 daidzein은 이들에 비해 낮은 활성을 나타냈다. 또한 melan-a cells에서 o-dihydroxyisoflavone 유도체들의 티로시나제 활성과 멜라닌 생성을 비교 측정하였다. 특히 Compound 1 (IC₅₀ = 11.21 ± 0.2 μM), 2 (IC₅₀ = 5.23 ± 0.6 μM)의 경우 compound 3과 daidzein에 비해 티로시나제 활성을 억제하는데 효과가 있었으며 또한 멜라닌 생성을 50 % 억제할 때의 결과에서 이들 화합물은 각각 12.23 ± 0.7 μM (1), 7.83 ± 0.7 μM (2)과 같이 멜라닌 생성 억제 효능을 나타내었다. 따라서 고숙성 된장에서 유래한 7,3',4'-trihydroxyisoflavone은 항산화 및 미백효과를 가지는 화장품 소재로써 사용 가능하리라 판단된다. Doenjang (Korean fermented soybean paste) is a unique fermented food in Korea. It has been traditionally manufactured from soybeans, by Jang Yang process. We focused on the newly formed compound in highly aged Doenjang and its biological activity. One new o-dihydroxyisoflavone, 7,3',4'-trihydroxyisoflavone and two known o-dihydroxyisoflavone derivatives were isolated from 5-year-old Doenjang and evaluated as potent antioxidant and whitening effect by comparing with other known isoflavone. 7,8,4'-Trihydroxyisoflavone (compound 1), 7,3',4'-trihydroxyisoflavone (compound 2) and 6,7,4'-trihydroxyisoflavone (compound 3) inhibited DPPH (diphenyl- 1-picrylhydrazyl) formation by 50 % at a concentration of 21.5 ± 0.2, 28.7 ± 0.4 and 32.6 ± 0.6 (IC₅₀) respectively, whereas daidzein showed weak DPPH radical scavenging activity. In superoxide scavenging effect were measured in one assay. Compound 1 (IC₅₀ = 18.10 ± 0.2 μM) and 2 (IC50 = 10.54 ± 0.4 μM) show significant inhibitory activity and greater effect than L-ascorbic acid. But compound 3 and daidzein showed lower inhibition activity. Also, o-dihydroxyisoflavone derivatives evaluated as potent inhibitors on tyrosinase activity and melanin formation in melan-a cells. Compound 1 (IC₅₀ = 11.21 ± 0.2 μM), compound 2 (IC₅₀ = 5.23 ± 0.6 μM) exhibited significant inhibitory effect on tyrosinase activity. Furthermore, those compounds are significantly suppressed the cellular melanin formation by 50 % at a concentration of 12.23 ± 0.7 μM (1) and 7.83 ± 0.7 μM (2). This result suggests that 7,3',4'- trihydroxyisoflavone from highly aged Doenjang could be used as an active ingredient for cosmetics.