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      • 四鹽化炭素 反覆投與 家兎에 對한 NH₄Cl負荷試驗

        申玩永,金箕洪 고려대학교 의과대학 1972 고려대 의대 잡지 Vol.9 No.1

        This study was undertaken to evaluate changes in blood levels of ammonia nitrogen (NG₃-N), CO₂ contents and urea nitrogen(BUN) following NH₄Cl administration on toxic hepatitis of rabbits induced by carbon tetrachloride (CCl₄). Fourty rabbits were devided into 4 groups. Group Ⅰ: One ㎖ of 30% CCl₄ in olive oil was injected subcutaneously 5 times at intervals of 2 days until the 12th experimental day in 14 animals. Two animals thus treated were sacrificed respectively on the 3rd, 6th, 9th, 12th, 15th, 22nd and 29th day. In addition 2 nontreated animals were sacrificed for control. Blood samples were drawn from each of 14 animals prior to CCl₄ injection for the determination of blood NH₃-N, glutamic pyruvic transaminase (GPT), BUN and serum iron. Group Ⅱ: Eight animals were similarly treated with CCl₄ and blood samples were drawn every 3rd day until 15th day, and again on 22nd and 29th for determination of the same. Group Ⅲ: Ammonium chloride tolerance tests were carried out in 8 normal animals and blood samples were drawn for determination of blood levels of NH₃-N, CO₂ contents and BUN before, and at 1/2, 1 1/2, and 2 1/2 hrs. following NH₄Cl administration. Group Ⅳ: Ammonium chloride tloerance tests were done in 8 animals administered with CCl₄, and blood samples were drawn for the same as in group Ⅲ. Results were as follows: 1. Active central necrosis of the hepatic lobules was observed only until the 12th experimental day. This necrotic area was almost completely replaced by regenerated hepatic cells in the 22nd day and repair was complete on the 29th day. 2. Normal blood levels of NH₃-N, GPT and serum iron were respectively 77.4±17.5㎍%, 36.7±7.9 Sigma-Frankel units and 130.3±33.6㎍%. 3. Average blood levels of NH₃-N, GPT and serum iron which were analyzed in 8 animals on every 3rd day after CCl₄ injection, were respectively 147.4㎍%, 259.6 Sigma-Frankel units, 18.9㎎% and 295.8㎍%. After the last CCl₄ injection blood levels of NH₃-N and BUN returned normal at 10 days, serum iron at 17 days but GPT levels were slightly higher than normal even at 17 days. 4. The NH₄Cl tolerance tests conducted in 8 normal animals showed the average blood levels of NH₃-N, CO₂ contents and BUN as follows: prior to, 1/2, 1 1/2, 2 1/2 hr. after NH₄Cl administration. Blood NH₃-N: 70.5, 143, 81, 73.8㎍%; CO₂ contents: 41.4, 38.1, 37.8, 36.8 vol%; BUN: 22.5, 23.6, 25.2, 27.1㎎%. 5. Average blood levels of NH₃-N, CO₂ contents and BUN in 8 animals following NH₄Cl administration on every 3rd day after CCl₄ injection were respectively as follows: prior to, 1/2, 1 1/2, 2 1/2 hr. after NH₄Cl. Blood NH₃-N: 126.6, 203, 137.6, 116㎍%; CO₂ contents: 42.7, 38.4, 38.3, 35.7 vol%; BUN: 19.6, 20.9, 20.8, 21.1㎎%. Blood ammonia levels were most significantly elevated at 1/2hr. after NH₄Cl loading in both CCl₄ treated and control groups, and elevation rates were 60% and 100%, respectively. However, elevated amount showed 76.4㎍% and 72.5㎍%. BUN levels were highest at 2 1/2hr. in both groups and elevation rates were 8% and 22%. Also elevation amount was 2.6㎎% and 4.6㎎%. CO₂ contents were most decreased at 2 1/2hr. in both groups and decrease rates were 16% and 12%, respectively.

      • 手術前後에 있어서 末稍血中 好酸球數의 變動

        申玩永 우석대학교 의과대학 1968 우석의대잡지 Vol.5 No.1

        In 32 cases, peripheral eosinophil counts per 1 cubic millimeter revealed 475.9±176.2 1 hour before operation, 353.5±195.4, 228.8±144.5, 163.8±100.0, 219.8±125.9, 250.9±119.2 and 248.0±136.1 immediately after, 3, 6, 12, 24 and 48 hours after operation. The eosinophil count was most decreased 6 hours after surgery.

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