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재래종 황색자두효소 갈변반응 생성물의 돌연변이 억제작용
함승시 한국농화학회 1987 Applied Biological Chemistry (Appl Biol Chem) Vol.30 No.1
The mutagenicity and desmutagenicity on enzymatic browning reaction products which obtained from prunes sazlicina (yellow) enzyme and polyphenol compounds were carried out. In the rec-assay on Bacillus subtilis strains H17 and M45, the enzymatic browning reaction products of pyrogallol, hydroxyhydroquinone, 3,4-dihydrotytoluene and catechol of 10^(-2)M did not showed mutagenicity. In the effects of various metal ions on the rec-assay, the enzymatic browning reaction products of pyrogallol showed mutagenic activity by Fe^(3+), Mn^(2+), Zn^(2+), Ni^(2+) and Al^(3+). In the enzymatic browning reaction products of hydroxyhydroquinone, Cu^(2+), Mn^(2+) and Pb^(2+) were effected in mutagenic action and the enzymatic browning reaction products of catechol was effected in mutagenic action by Mn^(2+). In the DNA-breaking action of enzymatic browning reaction products of pyrogallol, hydroxyhydroquinone, 3,4-dihyroxy-oluene and catechol did not show, DNA-breaking action. In the effects of various metal ions on the DNA-breaking action of enzymatic browning reaction products, Cu^(2+) showed DNA-breaking action. In the mutagenicity test on Sal. typhimurium strains TA98 and TA 100 with S-9 mix, 4 kinds of browned substances did sot shove muragenicity, all the browned substances showed strong desmutagenic activity in the presence of benzo (α)-pyrene with S-9 mix.