Quantitative trait loci analysis of morphological traits in Citrus

Mehtap S¸ ahin-C¸ evik,Gloria A. Moore 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.1

The objectives of this study were to understand the genetic basis of morphological variation observed in the genus Citrus and its relatives and to identify genomic regions associated with certain morphological traits using genetic linkage mapping and quantitative trait loci (QTLs) analysis with random amplified polymorphic DNA (RAPD) markers. First, a genetic linkage map was constructed with RAPD markers obtained by screening 98 progeny plants from a {Citrus grandis 9 [C. paradisi 9 Poncirus trifoliata]} 9 {[(C. paradisi 9 P. trifoliata) 9 C. reticulata] 9 [(C. paradisi 9 Poncirus trifoliata) 9 C. sinensis]} intergeneric cross. The map contains 69 RAPD markers distributed into nine linkage groups. Then, 17 different morphological traits, including six tree and two leaf characters of 98 progeny plants and six floral and three fruit characters of about half of the same progeny plants were evaluated for 2 years and statistically analyzed for variation. Statistical analysis of individual traits indicated that trunk diameter and growth, tree height, canopy width, tree vigor and growth, leaf length and width, petal and anther numbers, petal length and width, length of pistil and style, fruit length and diameter, and fruit segment number showed normal or close to normal distribution, suggesting that these traits may be inherited quantitatively. Quantitative data from the morphological traits were analyzed to detect markers and putative QTLs associated with these traits using interval mapping method. QTL analysis revealed 18 putative QTLs of LOD[3.0 associated with 13 of the morphological traits analyzed. The putative QTLs were distributed in several different linkage groups, and QTLs associated with similar traits were mostly mapped to the same LG or similar locations in the linkage group, indicating that the same genomic region is involved in the inheritance of some of the morphological traits.

The Effects of Alendronate and Calcitonin on Cytokines in Postmenopausal Osteoporosis: A 6-Month Randomized and Controlled Study

AliG?r,AzizDenli,Remzi?evik,KemalNas,MehmetKarako?,AysegulJaleSara? 연세대학교의과대학 2003 Yonsei medical journal Vol.44 No.1

The isomorphism between two fundamental groups by Cayley graphs

˙I. Naci Cang¨ul,A. Sinan C¸ evik,¨Ozden Koruo˘glu 장전수학회 2008 Advanced Studies in Contemporary Mathematics Vol.17 No.1

LetG1 and G2 be two nite groups and letCay(G1,S1) and Cay(G2,S2) be the corresponding Cayley graphs of these groups, respectively. By [2] and [8], one can dene the fundamental groupπ1(Γ,v) by using any connected graph Γ with a fixed vertex v. In this paper we give sufficient conditions for any two fundamental groups which are obtained by Cayley graphs Cay(G1,S1) and Cay(G2,S2) to be isomorphic. At the final part of the paper, we present some examples of this result.

Complications of Brucellosis in Different Age Groups: A Study of 283 Cases in Southeastern Anatolia of Turkey

AliG?r,MehmetFarukGeyik,BunyaminDikici,KemalNas,Remzi?evik,JaleSara?,SalihHosoglu 연세대학교의과대학 2003 Yonsei medical journal Vol.44 No.1

On the Laplacian-energy-like invariant

Das, Kinkar Ch.,Gutman, Ivan,Ç,evik, A. Sinan Elsevier 2014 Linear Algebra and its Applications Vol.442 No.-

<P>Let G be a connected graph of order n with Laplacian eigenvalues mu(1) >= mu(2) >= ... mu(n-1) >mu(n) = 0. The Laplacian-energy-like invariant of the graph G is defined as LEL = LEL(G) = Sigma(n-1)(i=1)root mu(i) . Lower and upper bounds for LEL are obtained, in terms of n, number of edges, maximum vertex degree, and number of spanning trees. (C) 2013 Elsevier Inc. All rights reserved.</P>

Spinal Cord Compression by Primary Amyloidoma of the Spine

KemalNas,AdemArslan,AdnanCeviz,AslanBilici,AliG?r,MustafaSerdarKemalo?lu,Remzi?evik,Ayseg?lJaleSara? 연세대학교의과대학 2002 Yonsei medical journal Vol.43 No.5

Tax Structure and Economic Growth: A Panel Data from OECD Countries

Savaş Çevik,오창규 경남대학교 산업경영연구소 2013 지역산업연구 Vol.36 No.1

This study examines the long-run relationship between tax structure and GDP per capita by based on panel data of 26 OECD countries over the period 1980-2010. Hausman specification test to verify the differences between PGM and MG was applied and the residuals of the equations was tested for stationary. The results suggest that income taxes are generally associated with lower economic growth, except for corporate income tax. On the other hand, consumption taxes in all types are associated with higher growth rate. In all specifications, taxes on personal income are negatively associated with GDP per capita, while shifting to consumption taxes positively affects the growth. In the perspectives of revenue-neutral analyses, we reveal the robust evidence on favoring consumption taxes over taxes on personal income (the sum of personal income tax, social security contribution and payroll taxes) with respect to growth.

Potentiometric urea biosensor based on poly(glycidylmethacrylate)-grafted iron oxide nanoparticles

Emre Çevik,Mehmet S¸ enel,Abdülhadi Baykal 한국물리학회 2013 Current Applied Physics Vol.13 No.1

Urease enzyme was covalently attached on the poly(glycidylmethacrylate) (PGMA)-grafted iron oxide nanoparticles on Au electrode for the fabrication of urea biosensor. The telomere of poly(- glycidylmethacrylate) (PGMA) with a trimethoxysilyl terminal group was synthesized by telomerization of glycidylmethacrylate. Iron oxide nanoparticles were coated with telomere of poly(- glycidylmethacrylate) in order to obtain good enzyme immobilization platform. The telomere and nanoparticles were characterized by using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and thermal gravimetric analysis (TGA). The biosensor’s potentiometric response was measured as a function of urea concentration in phosphate buffer solution (10 mM, pH 7.5) and showed a linear range of 0.25e5.0 mM urea. The produced biosensor exhibited a good response time of w8 s and was stable for about two months. The basic features (optimum pH, optimum temperature, interference and storage stability) of the enzyme electrode were determined

The First Identified Citrus tristeza virus Isolate of Turkey Contains a Mixture of Mild and Severe Strains

Bayram Çevik,Nejla Yard mc,Sava Korkmaz 한국식물병리학회 2013 Plant Pathology Journal Vol.29 No.1

The presence of Citrus tristeza virus (CTV) has previously been reported in citrus growing regions of Turkey. All serologically and biologically characterized isolates including I d r, which was the first identified CTV isolates from Turkey, were considered mild isolates. In this study, molecular characteristics of the I d r isolate were determined by different methods. Analysis of the I d r isolate by western blot and BD-RT-PCR assays showed the presence of MCA13 epitope, predominantly found in severe isolates, in the I d r isolate revealing that it contains a severe component. For further characterization,the coat protein (CP) and the RNA-dependent RNA polymerase (RdRp) genes representing the 3'and 5' half of CTV genome, respectively, were amplified from dsRNA by RT-PCR. Both genes were cloned separately and two clones for each gene were sequenced. Comparisons of nucleotide and deduced amino acid sequences showed that while two CP gene sequences were identical, two RdRp clones showed only 90% and 91% sequence identity in their nucleotide and amino acid sequences, respectively, suggesting a mixed infection with different strains. Phylogenetic analyses of the CP and RdRp genes of I d r isolate with previously characterized CTV isolates from different citrus growing regions showed that the CP gene was clustered with NZRBTH30,a resistance breaking isolate from New Zealand,clearly showing the presence of severe component. Furthermore, two different clones of the RdRp gene were clustered separately with different CTV isolates with a diverse biological activity. While the RdRp-1 was clustered with T30 and T385, two well-characterized mild isolates from Florida and Spain, respectively, the RdRp-2 was most closely related to NZRB-G90 and NZRB-TH30, two well-characterized resistance breaking and stem pitting (SP) isolates from New Zealand confirming the mixed infection. These results clearly demonstrated that the I d r isolate, which was previously described as biologically a mild isolate, actually contains a mixture of mild and severe strains.

Detection of Viruses Infecting Stone Fruits in Western Mediterranean Region of Turkey

Bayram Çevik Nejla Yardimci,Handan Çulal-Klllç 한국식물병리학회 2011 Plant Pathology Journal Vol.27 No.1

Field surveys were conducted in 45 stone fruit orchards in seven districts of Isparta Province located in western Mediterranean region of Turkey important for stone fruit production. Leaf samples were collected from 175trees showing virus-like symptoms. These samples were first tested by ELISA for five different RNA viruses including Apple mosaic ilarvirus (ApMV), Prunus necrotic ringspot ilarvirus (PNRSV), Prune dwarf ilarvirus (PDV),Plum pox potyvirus (PPV), Apple chlorotic leafspot trichovirus (ACLSV). While no ApMV and PPV infection was found, 46, 24 and 16 samples were tested positive for PDV, ACLSV and PNRSV, respectively, in ELISA showing about 45% of symptomatic trees in the region were infected with at least one of these viruses. In addition,it was found that nine sweet cherry trees were mixed infected with two or three of these viruses and PDV with an infection rate of 26.3% was the most widespread virus in symptomatic trees in western Mediterranean region. Thirty samples were selected and tested by a multiplex RT-PCR (mRT-PCR) for simultaneous detection of these viruses. While PPV was not detected, more than half of the tested 20 samples were individually or mixed infected with ApMV, ACLSV, PNRSV and PDV. The mRT-PCR results were confirmed by detection of these viruses individually in some of the field samples using RT-PCR with primes specific to each virus. Comparison of ELSA and mRT-PCR results of 30 samples showed that numbers of infected and mixed infected samples as well as infection and mixed infection rates were significantly higher in RT-PCR (20 and 66.7%)than in ELISA (14 and 46.7%). The results confirm that mRT-PCR is more sensitive than ELISA.