A Simple Method for Cryopreservation of Human Periodontal Ligament Cells in a Vitrification Solution Containing 20% Ethylene Glycol and 20% Dimethyl Sulfoxide

( Yemi Kim ),( Myung Rae Kim ),( Jung Hyuck Ahn ),( Sun Jong Kim ) 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.4

Vitrification approaches for the cryopreservation of human periodontal ligament (PDL) cells have notyet been successfully standardized, although vitrification has been widely used in various cells and tissues. The aims of this study were to examine whether vitrification approaches could be used for the cryopreservation of human PDL cellsand to establish the most suitable vitrification formula for tooth cryopreservation. Human PDL cells were cryopreserved with 4 solutions: VS1, 20% (v/v) ethylene glycol (EG) and 20% dimethyl sulfoxide (Me2SO); VS2, 40% EG; VS3,20% (v/v) 1,2-propanediol (PROH) and 20% (v/v) Me2SO; and VS4, 40% PROH. All vitrification solutions contained 0.6M sucrose and 18% (w/v) Ficoll 70 as non-permeating cryoprotectants. Cells were equilibrated in a 25% concentrationof each vitrification solution for 3 minutes at room temperature and exposed to each full strength solution at 4oC.The cells were then immediately transferred to liquid nitrogen and cryopreserved for 7 days. After stepwise warming with 0.5, 0.25 and 0.125M sucrose and F-medium, cell viability was measured using trypan blue exclusion, MTTassay, Cell Counting Kit-8 assay and morphological appearance. Cell apoptosis was assessed using TUNEL assay and Annexin V assay. VS1 led to 93% viability which is significantly higher than other groups. Morphological appearance and the percentage of apoptotic cells were similar between the VS1 and control groups. These results indicate that the vitrification method may be applicable to the cryopreservation of PDL cells and that VS1 can be used in successful tooth cryopreservation.

Histomorphometric Study on Healing of Critical Sized Defect in Rat Calvaria using Three Different Bovine Grafts

( Khvan Ekaterina ),( Yemi Kim ),( Ji Youn Kim ),( Myung Rae Kim ),( Sung O Kim ),( Sun Jong Kim ) 한국조직공학·재생의학회 2012 조직공학과 재생의학 Vol.9 No.5

The objective of this study was to evaluate the feasibility of a new xenograft material Ti-Oss (Chiyewon, Guri, Korea) by investigating the degree of new bone formation compared with that of other commonly used xenografts Bio-Oss(R)(Geistlich Pharma AG, Wolhusen, Switzerland) and OCS-B(R)(NIBEC, Seoul, Korea). Forty rats were used in the study. The animals were randomly allocated into four groups of 10 animals each. The Group 1`s calvarial defect was untreated, Group 2`s defect was filled with Bio-Oss(R)(Geistlich Pharma AG), Group 3`s defect was filled with OCS-B(R)(NIBEC), and Group 4`s defect was filled with Ti-Oss(R)(Chiyewon). The animals were sacrificed at 2 (n=5) and 8 (n=5) weeks for histomorphometric analysis. The histomorphometric analysis showed the statistically significant difference between the groups with a mean bone formation of 0.35±0.10 mm (8.75%) for Bio-Oss(R) group, 0.19±0.04 mm (4.75%) for OCS-B(R) group, 0.26±0.04 mm (6.5%) for Ti-Oss(R) group at 2 week healing period. At 8 weeks, a mean bone formation of 1.93±0.18 mm (48.25%) for Bio-Oss(R) group, 1.12±0.11 mm (28%) for OCS-B(R) group, 1.50±0.28 mm (37.5%) for Ti-Oss(R) group showed the statistically significant difference (p < 0.05). Within the limits of present study, it was concluded that the newly investigated xenograft, Ti-Oss(R), revealed biocompatibility and effectiveness in bone formation. Ti-Oss(R) could be proposed as a potential material for filling osseous defects. Further clinical trials of the new bovine xenograft Ti-Oss(R) are needed for an understanding its relevance in dentistry.

Morphological Characteristics and Classification of Mandibular First Molars Having 2 Distal Roots or Canals: 3-Dimensional Biometric Analysis Using Cone-beam Computed Tomography in a Korean Population

Kim, Yemi,Roh, Byoung-Duck,Shin, Yooseok,Kim, Bom Sahn,Choi, Yoo-lim,Ha, Aena Elsevier 2018 JOURNAL OF ENDODONTICS - Vol.44 No.1

<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>The aim of this study was to determine the morphologic characteristics of mandibular first molars having 2 canals in distal roots. Interorifice distance, buccal bone thickness, and root curvature were evaluated using cone-beam computed tomography images in a Korean population.</P> <P><B>Methods</B></P> <P>In total, 1958 mandibular first molars were evaluated in axial, coronal, sagittal, and paraxial planes. Distal roots having 2 canals were classified according to their root and canal shapes (2 roots, 2 canals [2R2C]; 1 root, 2 canals with 2 apical foramina [1R2C(2-2)]; and 1 root, 2 canals with 1 apical foramen [1R2C(2-1)]). The distances between orifices and the distance from the apex to the buccal bone plate were measured for each root canal shape (2R2C, 1R2C[2-2], and 1R2C[2-1]). The curvature of distolingual (DL) roots was classified according to severity using 3-dimensional reconstructed images, and the direction of curvature was determined. The relationships of these characteristics to sex and side were evaluated.</P> <P><B>Results</B></P> <P>The prevalences of 2R2C, 1R2C(2-2), and 1R2C(2-1) were 25.89%, 10.32%, and 14.15%, respectively. The distances between distobuccal (DB) and DL orifices were 3.77 ± 0.74 mm for 2R2C, 3.02 ± 0.65 mm for 1R2C(2-2), and 2.44 ± 0.64 mm for 1R2C(2-1). The distances from the buccal plate to the DB canal were 3.84 ± 1.35 mm for 2R2C, 5.33 ± 1.41 mm for 1R2C(2-2), and 5.96 ± 1.63 mm for 1R2C(2-1). The distance from the buccal plate to the DL canal was 9.85 ± 1.46 mm for 2R2C, and 8.28 ± 1.50 mm for 1R2C(2-2). All distances differed significantly according to root canal configurations, and all were greater in men than women (<I>P</I> < .05), except for the DB-DL orifice distance in 1R2C(2-2) and the DB to buccal cortical plate distance in all root configurations (<I>P</I> > .05). No significant difference between the left and right sides was found (<I>P</I> > .05). The prevalence of most severely curved DL roots (type III) was 62.92%, and the direction was commonly toward the buccal side (69.03%).</P> <P><B>Conclusions</B></P> <P>The prevalence of mandibular first molars having 2 canals in distal roots was more than 50% in a Korean population. Interorifice distances between DB and DL canals and distances from the apex to the buccal cortical plate differed according to root and canal numbers and shapes.</P>

안드로이드 스마트폰 정보 추출 방법에 관한 연구

김예미(Yemi Kim),유희정(HeeJeong Yoo),이윤영(Yoonyoung Lee),조진성(JinSung Cho) 한국정보과학회 2019 한국정보과학회 학술발표논문집 Vol.2019 No.12

Glucose metabolism of visceral adipose tissue measured by 18F-FDG PET/CT is related to the presence of colonic adenoma

Yoon, Hai-Jeon,Kim, Bom Sahn,Lee, Ko Eun,Moon, Chang Mo,Yoo, Jang,Kim, Jung-Sook,Kim, Yemi Williams & Wilkins Co 2017 Medicine Vol.96 No.25

ABSTRACT: This study investigated the relationships between the area and metabolic activity of adipose tissue and the presence of colorectal adenoma (CRA). Our institutional review board approved the study and waived informed consent. A total of 212 subjects who underwent fluorine 18 fluorodeoxyglucose (FDG) positron emission tomography (PET)/computed tomography (CT) and colonoscopy for routine health check-ups were enrolled. The volumetric parameters of areas of visceral (VATav), subcutaneous (SATav), and total adipose tissue (TATav) and calculated visceral-to-subcutaneous adipose tissue ratio (VSR) and visceral-to-total adipose tissue ratio (VAR) were considered. Metabolic parameters of standardized uptake value (SUV) of visceral (vcSUVmax, vcSUVmean), subcutaneous (scSUVmax, scSUVmean), and calculated visceral-to-subcutaneous adipose tissue ratio (VSRmax, VSRmean) were considered. Anthropometric data of height, weight, body mass index (BMI), waist circumference (WC), body fat mass (BFM), skeletal muscle mass (SMM), and diverse laboratory data were also considered as variables. Sixty-six subjects were placed in the CRA group and 146 subjects in the non-CRA group. The presence of CRA was significantly correlated with older age (P  =  .001), male sex (P  =  .041), higher BMI (P  =  .004), higher WC (P  =  .001), higher BFM (P  =  .024), higher VATav (P < .001), higher TATav (P  =  .004), higher VSR (P < .001), higher VAR (P < .001), lower vcSUVmax (P  =  .002), lower vcSUVmean (P < .001), and lower VSRmean (P  =  .002). On multiple regression analysis, vcSUVmax and vcSUVmean were independently associated with the presence of CRA (P  =  .009 and P  =  .045). Lower glucose metabolism of visceral adipose tissue was related to the presence of CRA. Our findings identify the value of visceral metabolic dysfunction as a potential surrogate marker of elevated risk for CRA.

Accidental injury of the inferior alveolar nerve due to the extrusion of calcium hydroxide in endodontic treatment: a case report

Shin, Yooseok,Roh, Byoung-Duck,Kim, Yemi,Kim, Taehyeon,Kim, Hyungjun The Korean Academy of Conservative Dentistry 2016 Restorative Dentistry & Endodontics Vol.41 No.1

During clinical endodontic treatment, we often find radiopaque filling material beyond the root apex. Accidental extrusion of calcium hydroxide could cause the injury of inferior alveolar nerve, such as paresthesia or continuous inflammatory response. This case report presents the extrusion of calcium hydroxide and treatment procedures including surgical intervention. A 48 yr old female patient experienced Calcipex II extrusion in to the inferior alveolar canal on left mandibular area during endodontic treatment. After completion of endodontic treatment on left mandibular first molar, surgical intervention was planned under general anesthesia. After cortical bone osteotomy and debridement, neuroma resection and neurorrhaphy was performed, and prognosis was observed. But no improvement in sensory nerve was seen following surgical intervention after 20 mon. A clinician should be aware of extrusion of intracanal medicaments and the possibility of damage on inferior alveolar canal. Injectable type of calcium hydroxide should be applied with care for preventing nerve injury. The alternative delivery method such as lentulo spiral was suggested on the posterior mandibular molar.

와동의 형태가 상아질과 복합레진 사이의 미세인장결합강도에 미치는 영향

김예미,박정원,이찬영,송윤정,서덕규,노병덕 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.5

이 실험의 목적은 동일한 깊이의 상아질에서 제 6세대의 self-etching system을 사용하여 C-factor가 상아질과 복합레진 사이의 미세인장결합강도에 미치는 영향을 알아보는 것이다. 건전한 대구치 80개를 선정하여 와동의 바닥면적은 각각 16㎟로 동일하게 하고 깊이를 조절하여 C-factor가 각각 0.25, 2, 3, 4인 4개의 군으로 나누었다. 각 군별로 총 20개의 치아를 할당하였으며 접착제와 복합레진의 조합에 따라 다시 4개의 소군에 치아 5개씩을 배정하였다. 상아질 접착제는 AQ Bond Plus또는 Xeno III를 사용하였고, 복합레진은 Fantasista또는 Ceram-X mono를 사용하였다. 제조사의 지시대로 상아질 접착제를 도포한 후 60초간 광중합하였다. 24시간 동안 증류수에 보관 후 단면적이 1.0 x 1.01㎟인 복합레진-상아질 beam을 형성하여 1 mm/min의 속도로 미세 인장결합강도를 측정하였다. One-way ANOVA와 Tukey test, 그리고 Pearson correlation test로 통계처리하여 다음과 같은 결과를 얻었다. l. Xeno III와 Ceram-X mono군에서는 C-factor가 증가할수록 미세인장결합강도가 유의하게 감소했으나 (p < 0.05), 나머지 재료군에서 C-factor군간에 미세인장결합강도의 유의한 차이가 없었다. 2. C-factor 3군에서는 AQ Bond Plus와 Fantasista군이 XenoIII와 Ceram-X mono군에 비해 높은 결합력을 보였으나 (p < 0.05), C-factor 0.25군, 2군, 4군에서 4가지 접착제와 수복 레진의 조합 간에 유의한 차이가 없었다. 3. Fantasista군에서 C-factor와 미세인장결합강도 사이에 일정한 상관관계를 발견할 수 없었고, Ceram-X mono군에서 C-factor가 증가할수록 미세인장결합강도가 감소하는 경향을 보였다 (p < 0.05). 이번 연구의 결과로 상아질의 깊이가 동일하고 6세대의 self-etching system을 사용하여 복합레진을 충전하는 경우와동의 C-factor가 수복물의 미세인장결합강도에 크게 영향을 미치지 않음을 알 수 있었다. This study was conducted to evaluate the influence of the C-factor on the bond strength of a 6th generation self-etching system by measuring the microtensile bond strength of four types of restorations classified by different C-factors with an identical depth of dentin. Eighty human molars were divided into four experimental groups, each of which had a C-factor of 0.25, 2, 3 or 4. Each group was then further divided into four subgroups based on the adhesive and composite resin used. The adhesives used for this study were AQ Bond Plus (Sun Medical, Japan) and Xeno III (DENTSPLY, Germany). And composite resins used were Fantasista (Sun Medical, Japan) and Ceram-X mono (DENTSPLY, Germany). The results were then analyzed using one-way ANOVA, a Tukey's test, and a Pearson's correlation test and were as follows. 1. There was no significant difference among C-factor groups with the exception of groups of Xeno III and Ceram-X mono (p < 0.05). 2. There was no significant difference between any of the adhesives and composite resins in groups with C-factor 0.25, 2 and 4. 3. There was no correlation between the change in C-factor and microtensile bond strength in the Fantasista groups. It was concluded that the C-factor of cavities does not have a significant effect on the microtensile bond strength of the restorations when cavities of the same depth of dentin are restored using composite resin in conjunction with the 6th generation self-etching system.

상아질 접착제의 두께가 치아와 복합레진 경계의 응력발생에 미치는 영향에 관한 유한요소법 연구

박상일,김예미,노병덕 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.5

이 연구의 목적은 5급 와동의 복합레진 수복에서 상아질 접착제를 비교적 두껍게 도포하거나 낮은 탄성계수(low elastic modulus)를 가진 복합레진을 선택하는 방법을 택하여 수복물의 탄성율의 차이에 따른 치아와 복합레진 경계에서 발생하는 응력의 차이를 알아보는 것이었다. abfraction 병소가 있는 하악 제 1소구치를 유한요소법을 이용하여 모델로 만들었다. 병소는 상아질 접착제의 두께 (50㎛, 100㎛, 150㎛) 및 복합레진의 종류를 달리하며 수복되었다. 170N의 교합압을 협측 또는 설측에서 가하여 상아질 접착제와 치아면에서의 Von Mises stress를 측정하였다. 상아질 접착제의 두께가 두꺼울수록 상아질 접착제면에 가해지는 Von Mises stresss 는 감소하였다. 낮은 탄성계수 값을 가진 복합레진에서 Von Mises stress가 더 작았다. 치근상아질 경계 (margin)가 법랑질 경계에서보다 더 큰 응력이 발생하였다. 상아질 접착제면에 작용하는 교합압의 영 향을 감소시키기 위해 상아질 접착제를 두껍게 바르거나 낮은 탄성계수 값을 가진 복합레진을 사용하는 것이 추천된다. The aim of this study was to examine that thick dentin bonding agent application or low modulus composite restoration could reduce stresses on dentin bonding agent layer. A mandibular first premolar with abfraction lesion was modeled by finite element method. The lesion was restored by different composite resins with variable dentin bonding agent thickness (50㎛, 100㎛, 150㎛). 170N of occlusal loading was applied buccally or lingually. Von Mises stress on dentin bonding agent layer were measured. When thickness of dentin bonding agent was increased von Mises stresses at dentin bonding agent were decreased in both composites. Lower elastic modulus composite restoration showed decreased von Mises stresses. On root dentin margin more stresses were generated than enamel margin. For occlusal stress relief at dentin boning agent layer to applicate thick dentin bonding agent or to choose low elastic modulus composite is recommended.

Background 99mTc-methoxyisobutylisonitrile uptake of breast-specific gamma imaging in relation to background parenchymal enhancement in magnetic resonance imaging.

Yoon, Hai-Jeon,Kim, Yemi,Lee, Jee Eun,Kim, Bom Sahn Springer International 2015 EUROPEAN RADIOLOGY Vol.25 No.1

<P>Objectives This study investigated factors that could affect background uptake of Tc-99m-methoxyisobutylisonitrile (MIBI) on normal breast by breast-specific gamma imaging (BSGI). In addition, the impact of background Tc-99m-MIBI uptake on the diagnostic performance of BSGI was further investigated. Methods One hundred forty-five women with unilateral breast cancer who underwent BSGI, MRI, and mammography were retrospectively enrolled. Background uptake on BSGI was evaluated qualitatively and quantitatively. Patients were classified into non-dense and dense breast groups according to mammographic breast density. Background parenchymal enhancement (BPE) was rated according to BI-RADS classification. The relationship of age, menopausal status, mammographic breast density, and BPE with background 99mTc-MIBI uptake was analyzed. Results Heterogeneous texture and high background uptake ratio on BSGI were significantly correlated with younger age (p<0.001, respectively), premenopausal status (p<0.001 and p=0.003), dense breast (p<0.001, respectively), and marked BPE (p<0.001, respectively). On multivariate analysis, only BPE remained a significant factor for background MIBI uptake (p<0.001). There was a significant reduction in positive predictive value (p=0.024 and p=0.002) as background MIBI uptake and BPE grade increased. Conclusions BPE on MRI was the most important factor for background MIBI uptake on BSGI. High background MIBI uptake or marked BPE can diminish the diagnostic performance of BSGI.</P>

Prognostic value of semi-quantitative tumor uptake on Tc-99m sestamibi breast-specific gamma imaging in invasive ductal breast cancer.

Yoon, Hai-Jeon,Kim, Yemi,Chang, Kyu-Tae,Kim, Bom Sahn Japanese Society of Nuclear Medicine 2015 Annals of nuclear medicine Vol.29 No.7

<P>This study investigated the prognostic value of preoperative breast-specific gamma imaging (BSGI) uptake measured by a semi-quantitative method in invasive ductal carcinoma (IDC).</P>