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- 저자 : ( Yanhong Xu ) (검색결과 4 건)
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Culture of a Whole Porcine Liver Ex Situ without Red Blood Cells
( Jing Dong ),( Lingling Xia ),( Hefang Shen ),( Congwen Bian ),( Sujin Bao ),( Ming Zhang ),( Yan Dai ),( Yanhong Xu ),( Qiru Xiong ),( Jianjian Xu ),( Lili Xu ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Liver transplantation is an effective approach to end-stage liver disease. Shortage of donor liver and increased waiting time for liver transplantation necessitate the development of an organ culture system by which livers can be cultured and maintained ex situ for a prolonged period of time. The aim of this work is to test whether cell culture condition in vitro could be used to culture whole livers ex situ without the use of erythrocytes. Methods: Eight castrated male land race/farm young porcine livers were exposed to 30 min warm ischemia and 30 min cold perfusion. Livers were isolated and connected to an ex situ liver culture system using a standard culture medium RPMI 1640 supplied with 10% of fetal calf serum and sufficient dissolved oxygen under a normothermic condition for 6 hours. Metabolic biomarkers, bile and urea production, hepatic cell viability, and histology analysis of biopsies were performed and analyzed. Results: Dissociated porcine hepatic cells survived and grew in vitro under the standard RPMI 1640 culture medium. When the same RPMI 1640 medium supplemented with 10% of FCS and sufficient oxygen was used to culture livers ex situ, over 98% of liver cells were viable for at least 6 hours during ex situ whole organ culture based on the results from biochemical assays. Conclusions: Our data demonstrate that the liver culture sys tem established in this work can be used to culture whole livers ex situ in the absence of erythrocytes.
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ADAPTIVE AUTHORITY ALLOCATION OF HUMAN-AUTOMATION SHARED CONTROL FOR AUTONOMOUS VEHICLE
Wu Yanhong,Wei Hanbing,Chen Xing,Xu Jin,Rahul Sharma 한국자동차공학회 2020 International journal of automotive technology Vol.21 No.3
Great advances had been achieved in the discipline of environmental perception, motion planning and control strategy implementation, however, fully autonomous vehicle is still far from large-scale commercial application. The concept of “human-automation shared control” provides a promising solution to enhance autonomous driving safety, to which great research effort has been contributed in recent years. Nevertheless, more attention should be given to the following aspects. The present shared control strategy either only considers the discontinuous switching control between driver and ADS or investigates the simple effect of driver’s behavior in specific scenarios. The adaptive authority allocation between the driver’s active assistance and ADS hasn’t been investigated yet. In this paper, a shared control experiment with driver’s active assistance is conducted in scheduled traffic scenarios to observe the state of vehicle and arm’ EMG signal. After that, we construct a feature classification algorithm for shared control authority by clustering the experimental data. Then, a SCS with incremental PID controller and 2 DOF vehicle dynamic model is proposed. For validation of the SCS, the comparison of vehicle performance for different control authority illustrates that SCS can allocate appropriate control authority to improve the safety.
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DsLCYB Directionally Modulated β-Carotene of the Green Alga Dunaliella salina under Red Light Stress
Lan Yanhong,Song Yao,Guo Yihan,Qiao Dairong,Cao Yi,Xu Hui 한국미생물·생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.12
Carotenoids, which are natural pigments found abundantly in wide-ranging species, have diverse functions and high industrial potential. The carotenoid biosynthesis pathway is very complex and has multiple branches, while the accumulation of certain metabolites often affects other metabolites in this pathway. The DsLCYB gene that encodes lycopene cyclase was selected in this study to evaluate β-carotene production and the accumulation of β-carotene in the alga Dunaliella salina. Compared with the wild type, the transgenic algal species overexpressed the DsLCYB gene, resulting in a significant enhancement of the total carotenoid content, with the total amount reaching 8.46 mg/g for an increase of up to 1.26-fold. Interestingly, the production of α-carotene in the transformant was not significantly reduced. This result indicated that the regulation of DsLCYB on the metabolic flux distribution of carotenoid biosynthesis is directional. Moreover, the effects of different light-quality conditions on β-carotene production in D. salina strains were investigated. The results showed that the carotenoid components of β-carotene and β-cryptoxanthin were 1.8- fold and 1.23-fold higher than that in the wild type under red light stress, respectively. This suggests that the accumulation of β-carotene under red light conditions is potentially more profitable.
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Wenshuai Zhu,Huaming Li,Yehai Xu,Bilian Dai,Hui Xu,Chao Wang,Yanhong Chao,Hui Liu 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.2
Three types of TiO2 were synthesized by a hydrolysis and calcination method. The catalysts were characterizedby X-ray powder diffraction (XRD), diffuse reflectance spectrum (DRS), Raman spectra, and X-ray photoelectronspectroscopy (XPS). The XRD and Raman spectra indicated that amorphous TiO2 was successfully obtained at100 oC. The results indicated that amorphous TiO2 achieved the highest efficiency of desulfurization. The photocatalyticoxidation of dibenzothiophene (DBT), benzothiophene (BT), 4,6-dimethyldibenzothiophene (4,6-DMDBT) anddodecanethiol (RSH) in model oil was studied at room temperature (30 oC) with three catalysts. The system containedamorphous TiO2, H2O2, and [Bmim]BF4 ionic liquid, ultraviolet (UV), which played vitally important roles in the photocatalyticoxidative desulfurization. Especially, the molar ratio of H2O2 and sulfur (O/S) was only 2 : 1, which correspondedto the stoichiometric reaction. The sulfur removal of DBT-containing model oil with amorphous TiO2 couldreach 96.6%, which was apparently superior to a system with anatase TiO2 (23.6%) or with anatase - rutile TiO2 (18.2%). The system could be recycled seven times without a signicant decrease in photocatalytic activity.
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