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A Real-Time Surveillance System for Vaccine Cold Chain Based o n Internet of Things Technology
Shao-jun Jiang,Zhi-lai Zhang,Wen-yan Song 한국정보처리학회 2023 Journal of information processing systems Vol.19 No.3
In this study, a real-time surveillance system using Internet of Things technology is proposed for vaccine coldchains. This system fully visualizes vaccine transport and storage. It comprises a 4G gateway module, lowpowerand low-cost wireless temperature and humidity collection module (WTHCM), cloud service softwareplatform, and phone app. The WTHCM is installed in freezers or truck-mounted cold chain cabinets to collectthe temperature and humidity information of the vaccine storage environment. It then transmits the collecteddata to a gateway module in the radiofrequency_physical layer (RF_PHY). The RF_PHY is an interface forcalling the bottom 2.4-GHz transceiver, which can realize a more flexible communication mode. The gatewaymodule can simultaneously receive data from multiple acquisition terminals, process the received datadepending on the protocol, and transmit the collated data to the cloud server platform via 4G or Wi-Fi. Thecloud server platform primarily provides data storage, chart views, short-message warnings, and otherfunctions. The phone app is designed to help users view and print temperature and humidity data concerningthe transportation and storage of vaccines anytime and anywhere. Thus, this system provides a new vaccinemanagement model for ensuring the safety and reliability of vaccines to a greater extent.
Analysis of DDS Sampling Method and Harmonic Composition
Zhi-lai Zhang,Shao-jun Jiang,Li-li Liang 한국정보처리학회 2023 Journal of information processing systems Vol.19 No.2
Through theoretical proof and algorithm design, this paper numerically demonstrates that the three samplingmethods of DDS are equivalent in amplitude-frequency characteristics. Depending on theoretical analysis, thearticle obtains the conclusion that 2 points are optimal when sampling at 2, 3, and 4 points. Built on the dataresults, this paper obtains the fractional form of the amplitude and phase of the DDS sampled signal; in addition,this paper also obtains the design parameters of the DDS post-stage filter. It also gives a control method for thecalculation error when addressing this issue.
Guomin Han,Qian Shao,Cuiping Li,Kai Zhao,Li Jiang,Jun Fan,Haiyang Jiang,Fang Tao 한국미생물학회 2018 The journal of microbiology Vol.56 No.5
Aspergillus flavus often invade many important corps and produce harmful aflatoxins both in preharvest and during storage stages. The regulation mechanism of aflatoxin biosynthesis in this fungus has not been well explored mainly due to the lack of an efficient transformation method for constructing a genome-wide gene mutant library. This challenge was resolved in this study, where a reliable and efficient Agrobacterium tumefaciens-mediated transformation (ATMT) protocol for A. flavus NRRL 3357 was established. The results showed that removal of multinucleate conidia, to collect a homogenous sample of uninucleate conidia for use as the transformation material, is the key step in this procedure. A. tumefaciens strain AGL-1 harboring the ble gene for zeocin resistance under the control of the gpdA promoter from A. nidulans is suitable for genetic transformation of this fungus. We successfully generated A. flavus transformants with an efficiency of ~ 60 positive transformants per 106 conidia using our protocol. A small-scale insertional mutant library (~ 1,000 mutants) was constructed using this method and the resulting several mutants lacked both production of conidia and aflatoxin biosynthesis capacity. Southern blotting analysis demonstrated that the majority of the transformants contained a single T-DNA insert on the genome. To the best of our knowledge, this is the first report of genetic transformation of A. flavus via ATMT and our protocol provides an effective tool for construction of genome-wide gene mutant libraries for functional analysis of important genes in A. flavus.
AntagomiR-27a Targets FOXO3a in Glioblastoma and Suppresses U87 Cell Growth in Vitro and in Vivo
Ge, Yun-Fei,Sun, Jun,Jin, Chun-Jie,Cao, Bo-Qiang,Jiang, Zhi-Feng,Shao, Jun-Fei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2
Objective: To study the effect of the antagomiR-27a inhibitor on glioblastoma cells. Methods: The miR-27a expression level in specimens of human glioblastoma and normal human brain tissues excised during decompression for traumatic brain injury was assessed using qRT-PCR; The predicted target gene of miR-27a was screened out through bioinformatics databases, and the predicted gene was verified using genetic report assays; the effect of antagomiR-27a on the invasion and proliferation of glioma cells was analyzed using MTT assays and 5-ethynyl-2'-deoxyuridine (EdU) labeling. A xenograft glioblastoma model in BALB-c nude mice was established to detect the effect of antagomiR-27a on tumour growth. Results: qRT-PCR results showed that miR-27a significantly increased in specimens from glioblastoma comparing with normal human brain tissues. Th miR-27a inhibitor significantly suppressed invasion and proliferation of glioblastoma cells. FOXO3a was verified as a new target of miR-27a by Western blotting and reporter analyzes. Tumor growth in vivo was suppressed by administration of the miR-27a inhibitor. Conclusion: MiR-27a may be up-regulated in human glioblastoma, and antagomiR-27a could inhibit the proliferation and invasion ability of glioblastoma cells.
Kim, Young Koo,Lee, Seung Hun,Jiang, Shao Jun 대한피부과학회 1998 Annals of Dermatology Vol.10 No.3
Background: The stratum corneum presents a significant barrier to transdermal drug delivery. Approaches to improve percutaneous absorption of drugs have included iontophoresis and skin penetration enhancers. Oleic acid has been studied as a skin penetration enhancer for drugs, primarily via its action mainly on the stratum corneum lipid structure. Objective: The purpose of this study was to assess the interaction between oleic acid and stratum corneum lipids in vivo. Methods: Male hairless mice were treated topically with oleic acid. Barrier function was assessed by transepidermal water loss measurement and ultrastructural observation with ruthenium tetroxide (R_uO₄) staining. Results: Oleic acid in propylene glycol had a profound effect on epidermal barrier function and was found to be concentration dependent. Moreover, ultrastructural examination with R_uO₄ post-fixation demonstrated that there were marked alterations in the stratum corneum lipid structure. Conclusion: This study provides direct evidence that oleic acid increases the epidermal permeability through a mechanism involving the stratum corneum lipid membrane perturbation via the lacunae formation within the stratum corneum.
Wang, Kai,Wang, Yin-Yan,Ma, Jun,Wang, Jiang-Fei,Li, Shao-Wu,Jiang, Tao,Dai, Jian-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24
Several molecular markers have been proposed as predictors of outcome in patients with glioblastomas. We investigated the prognostic significance of $O^6$-methylguanine-DNA methyltransferase (MGMT) promoter methylation and TP53 mutation status dependent on isocitrate dehydrogenase 1 (IDH1) mutation in glioblastoma patients. A cohort of 78 patients with histologically confirmed glioblastomas treated with radiation therapy and chemotherapy were reviewed retrospectively. We evaluated the prognostic value of MGMT promoter methylation and TP53 mutation status with regard to progression-free survival (PFS) and overall survival (OS). It was revealed that mutations in IDH1, promoter methylation of MGMT, TP53 mutation, age, Karnofsky performance status (KFS), and extension of resection were independent prognostic factors. In patients with an IDH1 mutation, those with an MGMT methylation were associated with longer PFS (p=0.016) and OS (p=0.013). Nevertheless, the presence of TP53 mutation could stratify the PFS and OS of patients with IDH1 wild type (p=0.003 and 0.029 respectively, log-rank). The MGMT promoter methylation and TP53 mutation were associated with a favorable outcome of patients with and without mutant IDH1, respectively. The results indicate that glioblastomas with MGMT methylation or TP53 mutations have improved survival that may be influenced by IDH1 mutation status.
Water - Impermeable Occlusion Effect to Intercorneocyte Lipid Layers in Hairless Mice
Oh, Seaung Youl,Lee, Seung Hun,Jiang, Shao Jun 대한피부과학회 1997 Annals of Dermatology Vol.9 No.2
Background : Stratum corneum lipids are arranged as intercellular membrane bilayers presumed to mediate the epidermal permeability barrier. Acute disruption in barrier function will initiate epidermal lipid synthesis, which can be prevented by occlusive membrane. Whereas, occlusion of the skin is known to cause an increased transepidermal water loss(TEWL) and enhanced percutaneous absorption of a variety of compounds. Objective : Previous reports with electron microscopy showed varying sizes of lacunae and disorganized intercorneocyte lipids after tape stripping and occlusion with a water impermeable membrane on the murine skin. Hence we studied the effects on stratum corneum lipids and changes in barrier function after occlusion with a water-impermeable membrane. Methods : Male hairless mice were occluded with one finger of a Latex glove for 24, 48 and 60 hours. After occlusion, TEWL was measured and biopsy specimens were taken from skin. For electron microscopic examination the samples were treated with osmium tetroxide, ruthenitum tetroxide, and tracer (lanthanum) and infrared spectroscopy were also applied. Results : Occlusion with a water-impermeable membrane on the skin induced higher TEWL Values and greater penetration of the tracer than normal. Alterations of the lipid bilayer membrane and lacunae formation in the stratum corneum interstices were also induced after 24 hours of occlusion. However, the orderness of the lipid alkyl chain in the stratum corneum was not changed until 60 hours of occlusion. Conclusion : These studies indicate that the increased epidermal permeability after occlusion may be due to the abnormal lipid membrane structures and volume expansion of existing lacunar domains in the stratum corneum interstices.