동양달팽이의 눈에 대한 해부학적 및 미세구조적 연구

정계헌,이현 한국패류학회 1994 The Korean Journal of Malacology Vol.10 No.1

동양달팽이의 雄性 生殖器官에 대한 形態學的 硏究

이훈섭,정계헌,박종안 한국패류학회 1992 The Korean Journal of Malacology Vol.8 No.1

肝吸蟲의 宿主血液 攝取에 관한 硏究

朱東善,鄭啓憲,林漢鍾 고려대학교 의과대학 1982 고려대 의대 잡지 Vol.19 No.1

The purpose of this study was to confirm whether Clonorchis sinensis has an activity of blood intake from host during the course of development of the fluke in the body of the host. The following results were observed: 1. The observations were made weekly on the appearance and the amount of contents with black materials in the cecal lumen of worms which were removed from the bile duct of the rats infected experimentally with metacercariae of C. sinensis. only Tow weeks after the infection, the black materials were already observed in the cecal lumen, and the amount of it was increased in examinations at the 3rd and 4th week of infections. By the 5th week, the majority of worms found to have full cecal contents with black materials. 2. The black materials in the lumen of the intestinal ceca of C. sinensis were proved by benzidine test to be the degenerated products of hemoglobin. 3. Electronmicroscopic examination of the cecal contents of C. sinensis revealed the presence of blood cells. The blood cells found in the contents were morphologically all the same to the blood cells of the host rabbits. 4. It was easier to identify the leucocytes in microscopic fields than the erythrocytes, because the leucocytes retained somewhat normal shape of it, while the erythrocytes were almost degenerated. In the cecal contents there were also rich debris which supposed to be components of blood cells. Nothing other the probable blood components was identified. 5. Scanning electronmicroscopic examination demonstrated that the oral sucker of C. sinensis has the opening and a lip is thick with many fine transverse striae or wrinkles on their surface. Many mammillary papillae were there close to the oral sucker. The results of this study confirmed the belied that C. sinensis has an activity of blood intake from the host with the oral sucker.

긴촌충(廣節裂頭條蟲) 感柒의 一例

李駿商,林漢鍾,鄭啓憲 순천향대학교 1980 논문집 Vol.3 No.3

韓國에서의 긴촌충(廣節裂頭條史) 感染例는 日制時 慶南地方 住民들의 大便檢査 증에 史卵이 觀索되었다는 報告가 있었고, 患者로부터 成忠을 얻어 報告한 例는 趙等(1971)이 報告한 1例 뿐이었다. 著者들은 14歲의 韓國少年으로부터 길이 685cm의 頭節이 없는 史體를 얻었다. 治療藥製는 Bithionol이었다. 忠體를 形態學論으로 檢討한 바 Diphyllobothrium latum(Linnaeus, 1758) Luhe, 1910으로 同定되었다. 患者는 그때까지 外國을 旅行한 經驗이 없었으며 市中에서 生鮮膾률 먹거나 약수터 또는 溪流의 물을 마신 記憶이 없고 더우기 뱀이나 개구리를 生食한 經驗은 전혀 없다고 한다. 다만 그는 本硏究室을 찾기 1年前 그의 父親을 따라 浦項으로 바다낚시를 간 일이 있으며 그곳에서 낚아 올린 농어( 魚)를 謄로 먹은 經驗이 있다고 하였다. 그러므로 本例의 感染源은 魚일 가능성이 많으며 分明한 것은 國內의 土着感染이란 점이다. , 國內에서 수시로 報告되는 sparganum症의 例로 보거나 中間宿主의 役割을 할 수 있다고 思料되는 甲穀類 및 魚類에 대한 報告와 韓國海洋의 魚類分布로 보아 韓國에서의 廣節裂頭條忠 感染率은 窓外로 높을지도 모른다. A case of Diphyllobothrium latum infection was recorded. A 14-year-old Korean boy was infected with this tape worm and treated with bithionol orally. An incomplete strobila without scolex and neck parts was collected. It was identified as D.latum on the basis of morpho1ogical characteristics. The most probab1e source of infection is autochthonous according to the past history of the patient. This is the second record of Diphyllo-bothrium latum infection in Korea which was verified with the recovery of the adult worm.

나비目 終齡幼蟲의 血球에 關한 電顯的 硏究

梁凞永,李鳳熙,鄭啓憲 순천향의과대학 1979 Journal of Soonchunhyang Medical Science Vol.2 No.1

Morphological studies on larval hemocytes of varioius insects were performed in two ways of research. Ⅰ. Finestructures. An electron microscopical study on hemocytes of last instar larvae of 12 species of Lepidoptera was performed to observe cell types, structrues, raions, relationships and their origins. The results are as follows; 1) Prohemocytes are the smallest round cells with large numlesus, poorly developed cytoplasmic organelles and lower composition ratio, accounting for less than 5% of all cell types. Little remarked differences were revealed among prohemocytes of varous insects and they are thought to be the origins of other cell types. 2) Palsmatocytes are relatively large cells with higher composition ration, accounting for about 40%. They could be subdivided into two forms, oval and spindle. Oval cells are rich in cytoplasmic projections and organelles. But the spindle cells include few of them, while nearly all of the spindle cells possess some microtubules as their cytoskeletons. Some marked difference were revealed among different insects. The most exceptional structure was the fibrous bundles located in both nucleus and cytoplasm in Sericinus telamon Donovan. 3) Granular cells are characterized by various granules in cytoplasm, and by higher composition ration, accounting for about 50% of all numbers. They could be subdivided into three forms, oval, round and spindle with fully developed cytoplasmic organelles and Projections. Cellular fractions were often noticed to be enclosed by granular cells, indicating some activities of phagocytosis. Granular cells are the most pleomo rphous of all cell types, and the most exceptional structures are the bundles of microfibrils in Phalerodonta manleyi Leech. 4) Adipohemocyte were found only in Plodia interpunctella Hubner. They are somewhat oval with few cytoplamic projections and less developed organelles. Some granular cells were found to accumulate lipid droplets into the cytoplasm by trapping floating lipid droplets, adn the adipohemocytes are believed to be originated from formers. 5) Sperule cells were found nearly in all specimens and characterized by sperules composed of flocculent materials. They are round or oval with few cytoplasmic organelles. The most conspicuous spherulesof all sperule cells were radial arrangement of sperules in Adristyrannus amurensis Staudinger and presence of the specific granules in Mamestra illoba Butler. Young sperule cells resembled the granular cells and it is possible that they originate from granular cells. 6) Oenocytoids are large cells and were found nearly in all specimens. They could be subdivided into 3 types according to their inclusions, such as microfibrils, microtubules and none of them. Young Oenocytoids resemble the giant plasmatocytes, perhaps in dicating to be originated from the latters. Ⅱ. Composition ratios of cell types from mid 5-instar to late prepupa. Composition ratios of cell types were investigated in phalerodonta manley; Leech and could be summarized as follows; 1) Prohemocytes disappeared from the stage of early prepupa. 2) Plasmatocytes increased in number at mid stages of each inster and decreased nearing to pupal stage. 3) Granular cells reached peak at early prepupal stage. 4) Adipohemocytes heavily increased at late prepupal stage. 5) Sperule cells increased at late stage of each instar and reached peak at late prepupal stage. 6) Oenocytoids increased at late stage of each instar and reached peak at early prepupal stage.

An Ultrastructural Study on the Glochidium and Glochidial Encystment on the Host Fish

Jeong, Kye-Heon 한국패류학회 1989 The Korean Journal of Malacology Vol.5 No.1

Cytochemical and Immunocytochemical Study on the Cellulase Activity in the Accessory Glands of the Digestive System of the Oriental Land Snail, Nesiohelix

Jeong, Kye-Heon,Lee, Young-Seok,Shim, Yun-Bo The Malacological Society of Korea 1999 The Korean Journal of Malacology Vol.15 No.2

The histochemical, cytochemical, and immunocytochemical investigations were conducted to find out the cellulase activity in the accessory glands of the digestive system of the oriental land snail Nesiohelix samarangae under the LM, SEM, and TEM. The cellulase activity was shown in the epithelium of th digestive gland by labelling with the immunogold (protein-A gold) particles. The epithelial cells showing the cellulase activity were Type 1 and Type 3 cells out of five types of the epithelial cells of the digestive gland. None of epithelial cells of the mucus gland and the salivary gland and the salivary gland were not labeled with the immunogold particles.

A Study on the Fine Structure of Clonorchis sinensis, a Liver Fluke

Kye-Heon Jeong(鄭啓憲) 대한기생충학열대의학회 1983 The Korean Journal of Parasitology Vol.21 No.2

The Ultrastructure of the Spermatheca of the Pulmonate Snail Nesiohelix samarangae

Jeong, Kye-Heon The Malacological Society of Korea 1993 The Korean Journal of Malacology Vol.9 No.2

동양달팽이(Nesiohelix samarangae)의 자성생식기 중 정자낭(spermatheca)의 미세구조를 투과전자현미경을 이용하여 관찰하였다. 성체의 정자낭의 내강에서는 정자와 이와 관련된 물질들 또는 상피로부터의 분비물질들이 관찰되었다. 정자낭의 내강을 둘러싸고 있는 벽의 상피는 단층원주상피로서 내강과 연한 세포의 원형질막은 미세융모를 가지고 있었으며 섬모는 가지고 있지 않았다. 이 미세융모들의 기부에서는 고배율 하에서 pinocytotic vesicle들이 관찰되었고 때로 세포의 분비물을 세포 밖으로 방출하는 모습도 관찰되었다. 세포들의 상부세포질에는 많은 수의 mitochondria가 있었고, 때로 분비과립과 lysosomd들이 존재하기도 하였다. 이들보다 아래에는 잘 발달한 골지체와 과립소포체(RER)들이 있었다. 이 부위에서 분비과립들이 많이 형성되어 있는 모습이 세포에 따라 관찰되었고, 그 아래에 즉, 각 세포의 하반부 세포질에 상하로 긴 핵이 관찰되었다. 세포의 기부 원형질막은 상부 세포질 내로 깊게 그리고 수없이 많이 주름을 이루고 있어 인접한 세포와의 경계를 구분할 수 없을 정도였다. 기저막의 아래에는 근육다발과 색소세포들이 결합조직 내에서 관찰되었고, 정자낭의 외막은 큰 액포로 인하여 세포질들이 원형질막 주변으로 밀려나 있는 특이한 세포들로 이루어져 있었다. 정자낭의 기능은 정자를 일시 저장하며 정자낭 상피에서 분비되는 소화액들로 정자를 일차적으로 세포외 소화하고 소화되지 않은 물질들을 흡수하여 이차적으로 세포내소화하는 것으로 생각된다.

Glochidium larva의 부착으로 인한 숙주어류의 피양형성과정에 관한 주사전자현미경적 연구 (2)

Jeong, Kye-Heon,Oh, Young-Sook 한국패류학회 1991 The Korean Journal of Malacology Vol.7 No.1

A scanning electron microscopic stuey on the glochidial encystment study on the golchidal encystment and excystment of Anodonta fukudai on Acheilognathus yamatsutae, a common natural hostfish, was conducted. The glochidium easily attached to the unscaled surfaces of the host fish such as the fins, lips, and the wall of the buccal cavity. For this study, the fins infected with the glochidia wer mainly observed in a series. The process of encystment was slowly progressed, for 21-25 hours for the early cyst and for 2-4 days for the thick walled cyst. The process of excystmint was visually detected on the 12th day since the attachmint was occurred. The first visible sign was a little tear of the cyst wall covering the hinge and marginal zones of the juvenile clam and once the little sign was appeared the progress of emerging and dettachmint of the juvenile clam from the host was finished relatively in short time. During the process of the encystmint, the cells participationg in covering the attached glochidirm were seened mainly supplied by migration from the surroundings. the shapes of the cells migrating and covering the glochidium were considerably changed and the surface structures of the cells lost their normal pattern of the surface ridges. The unstable forms of the cells were observed almost all throughout the period of the glochidial attachment. No cells of the host epithelium, which were still attached to the juvenile clam energing from the cyst, were observed. The most juvenile clams escaped from the cysts were a little bigger than the glochidia and they were still possessed of the golchidial hooks even though much degenerated. The first growth line was appeared on the shell valves of the juvenild clam when observed right after dettachment.