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Ken-ichi Yoshida,Jan Maarten van Dijl 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.6
Bacillus subtilis has been studied for more than half a century, ever since the dawn of molecular biology, as a representative Gram-positive bacterium and cell factory. Two characteristic capacities of B. subtilis, namely its natural competence for DNA uptake and high-level enzyme secretion, have been investigated and exploited intensively during these long years. As a consequence, this bacterium has evolved into an excellent platform for synthetic biological research and development. In this review, we outline basic concepts for B. subtilis cell factory engineering, and we describe several examples of its applications in the production of proteins and high-value metabolites. In particular, we highlight engineering approaches that can make the already very efficient Bacillus protein secretion pathways even more efficient for the production of enzymes and pharmaceutical proteins. We further showcase examples of metabolic engineering in B. subtilis based on synthetic biology principles to produce various high-value or healthpromoting substances, especially inositol stereoisomers. We conclude that the versatile traits of B. subtilis, combined with multi-omics approaches and rapidly developing technologies for genome engineering and high-throughput screening enable us to modify and optimize this bacterium’s metabolic circuits to deliver compounds that are needed for a green and sustainable society as well as a healthy population.
KEN-ICHI TADA,SHIGENOBU SHINOHARA,HIROFUMI YOSHIDA,HIROAKI IKEDA,YASUHIRO SAITOH,KEN-ICHI NISHIDE,MASAO SUMI 대한전자공학회 1992 대한전자공학회 학술대회 Vol.1992 No.10
The measurable speed range of the self-mixing type semiconductor laser range finder has been greatly improved by employing a new processing circuit. Using this range finder as an external finder of a single lens reflex (SLR) autofocus (AF) camera, some clear photographs of an object moving at a medium speed of 20 ㎜/s is obtained.
Ken-ichi Funazaki,Nao Yoshida,Suzuna Saito 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.5
Demand for highly efficient gas turbine systems has been increasing TIT (turbine inlet temperature) and this trend will continue for forthcoming carbon-neutral age, necessitating the turbine designers to invent new ideas for adequate cooling of the turbine vanes and blades with less cooling air. Film cooling is one of the most effective cooling methods and a vast number of relevant studies have been made on this topic for decades. Recently several attempts including ones by the present authors have been made to investigate effects of flow control devices placed upstream or downstream of the cooling holes to improve film cooling effectiveness, showing promising results for their application to real gas turbines. This study proposes a new flow control device (FCD). This device takes V-shaped geometry on a flat plate to enhance the attachment of injected cooling air to the surface and it is placed very close to a cooling hole for the thermal management of the device itself. To maximize the aero-thermal performance of V-shaped FCD, numerical optimization supported by k-means clustering (Sing and Yang [1]) are made, and several excellent new V-shaped devices are found. Film cooling performance of the optimized devices results are validated by the experiment using PSP method.
( Suzuki Hirokazu ),( Ken Ichi Yoshida ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9
We established an efficient transformation method for thermophile Geobacillus kaustophilus HTA426 using conjugative transfer from Escherichia coli of host-mimicking plasmids that imitate DNA methylation of strain HTA426 to circumvent its DNA restriction barriers. Two conjugative plasmids, pSTE33T and pUCG18T, capable of shuttling between E. coli and Geobacillus spp., were constructed. The plasmids were first introduced into E. coli BR408, which expressed one inherent DNA methylase gene (dam) and two heterologous methylase genes from strain HTA426 (GK1380-GK1381 and GK0343-GK0344). The plasmids were then directly transferred from E. coli cells to strain HTA426 by conjugative transfer using pUB307 or pRK2013 as a helper plasmid, pUCG18T was introduced very efficiently (transfer efficiency, 10-5-10-3 recipient-1), pSTE33T showed lower efficiency (10-7-10-6 recipient-1) but had a high copy number and high segregational stability. Methylase genes in the donor substantially affected the transfer efficiency, demonstrating that the host-mimicking strategy contributes to efficient transformation. The transformation method, along with the two distinguishing plasmids, increases the potential of G. kaustophilus HTA426 as a thermophilic host to be used in various applications and as a model for biological studies of this genus. Our results also demonstrate that conjugative transfer is a promising approach for introducing exogenous DNA into thermophiles.
Mechanical Analysis of Pion Capture Superconducting Solenoid System for COMET Experiment at J-PARC
Iio, Masami,Yoshida, Makoto,Ye Yang,Taekyung Ki,Okamura, Takahiro,Sasaki, Ken-ichi,Makida, Yasuhiro,Ogitsu, Toru,Mihara, Satoshi,Nakamoto, Tatsushi,Sugano, Michinaka,Terashima, Akio,Kawamata, Hiroshi IEEE 2017 IEEE transactions on applied superconductivity Vol.27 No.4
<P>KEK is currently constructing a long series of superconducting solenoid beamline for the COMET Phase-I experiment at the Hadron facility of J-PARC. The magnet system consists of a 5-T pion capture solenoid, a curved muon transport solenoid, and a large bore detector solenoid. The pion capture solenoid system consists of a large cold mass including four coils with an inner diameter of 1340 mm and a small cold mass including six small coils with an inner diameter of 500 mm. The peak magnetic field on the conductor reaches to 5.4 T at an operation current of 2700 A. This paper presents the design and structural details of a magnet, radiation-resistant coil, cold mass and vacuum vessel, apart from the results of the electromagnetic analysis in two-dimensional (2-D) and 3-D.</P>