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Grid-Free Localization Algorithm Using Low-Rank Hankel Matrix for Super-Resolution Microscopy
Min, Junhong,Jin, Kyong Hwan,Unser, Michael,Ye, Jong Chul IEEE 2018 IEEE TRANSACTIONS ON IMAGE PROCESSING - Vol.27 No.10
<P>Localization microscopy, such as STORM/PALM, can reconstruct super-resolution images with a nanometer resolution through the iterative localization of fluorescence molecules. Recent studies in this area have focused mainly on the localization of densely activated molecules to improve temporal resolutions. However, higher density imaging requires an advanced algorithm that can resolve closely spaced molecules. Accordingly, sparsity-driven methods have been studied extensively. One of the major limitations of existing sparsity-driven approaches is the need for a fine sampling grid or for Taylor series approximation which may result in some degree of localization bias toward the grid. In addition, prior knowledge of the point-spread function (PSF) is required. To address these drawbacks, here we propose a true grid-free localization algorithm with adaptive PSF estimation. Specifically, based on the observation that sparsity in the spatial domain implies a low rank in the Fourier domain, the proposed method converts source localization problems into Fourier-domain signal processing problems so that a truly grid-free localization is possible. We verify the performance of the newly proposed method with several numerical simulations and a live-cell imaging experiment.</P>
Fabrication of recombinant azurin self-assembled layer for the application of bioelectronic device.
Min, Junhong,Kim, Sang-Uk,Kim, Young Jun,Yea, Cheol-Heon,Choi, Jeong-Woo American Scientific Publishers 2008 Journal of nanoscience and nanotechnology Vol.8 No.10
<P>A novel immobilization method of cupredoxin azurin on a gold surface was developed without a chemical linker using recombinant technique. A recombinant protein with cysteine residue by site-directed mutagenesis (SDM) was designed and then directly self-assembled on Au surface. The layer of the functionalized protein immobilized is confirmed by surface plasmon resonance (SPR) and its surface morphology is analyzed by scanning tunneling microscopy (STM). The immobilization efficiency has been increased about 73.2%, as compared to that of wild type azurin. The electrochemical property of the fabricated thin layer was investigated by the cyclic voltammetry (CV). As a result, cysteine-substituted azurin can be used for making high quality protein film, and applied to the fabrication of nano-scale bioelectronics.</P>
The Micro-Total Analytical System for the Detection of Bacteria/Viruses
Min, Junhong,Baeumner, Antje 한국공업화학회 2003 Journal of Industrial and Engineering Chemistry Vol.9 No.1
Gene-based biosensors have allowed fast and specific detection of pathogenic bacteria and viruses in past decade. In addition to nucleic acid biosensors, DNA arrays or gene have been researched to detect sequence-specific information in a faster, simpler and cheaper manner or the micro-total analytical system (μ-TAS), also known as "Lab-on-a-Chip", not only incorporates detection but additionally sample preparation and a digital readout all in one device. A variety of fields have to be combined to realize these micro-total analysis systems. Thus, several biological, chemical and engineering techniques are introduced in this paper to develop μ-TAS for the detection of viruses and bacteria. This short review article on the fabrication of μ-TAS for the detection of bacteria and viruses intended to provide an overview of the design criteria and potential fabrication techniques. Obviously, there are several different ways of realizing a successful μTAS, and only a limited number of examples were presented in this paper. Detection mechanisms such as microarrays, which can detect DNA/RNA molecules or proteins, are already commercialized by several companies (Nanogenu®, BioMicro systems™). They typically use silicon, or glass with standard photolithography techniquies and can even be connected to PDMS with molding and soft lithography to create channels and reactors. However, these arrays only represent the detection part of an entire μTAS, Fabricating μ TAS devices is technologically no challenge anymore, especially since seldomly submicrometer dimensions are required. However, the design of a functional μ-TAS and the integration of the diffcrent modules into one system are main issues to be consdered in the future. Given the impressive progress in the field of μ-TAS, there is no doubt that it will be the major sensing device for clinical diagnostic as well as for the detection of bacteria and viruses in the environment and for food safety.
( Junhong Min ),( Seungmyeong Chung ),( Minjung Kim ) 서암순창장학회 2017 Journal of Marketing Thought Vol.4 No.1
In a competitive environment, suppliers are an increasingly important resource for buyers, and buyer-supplier integration is essential to the entire business strategy. However, the relationship of integration with new product development remains vague, especially in a situation with structural holes. This research investigates how buyer-supplier integration influences new product development under conditions with structural holes. A network variable is included to develop a conceptual model that provides the perspective of the inter-firm relationship. As a result, this research can suggest a predictable direction, providing theoretical and practical implications.