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Lee, Youngjoo,Kim, Jiyeon,An, Jinho,Lee, Sungwon,Lee, Heetae,Kong, Hyunseok,Song, Youngcheon,Choi, Hye Ran,Kwon, Ji-Wung,Shin, Daekeun,Lee, Chong-Kil,Kim, Kyungjae The Korean Society of Applied Pharmacology 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.2
Hyperlipidemia, which is closely associated with a fatty diet and aging, is commonly observed in the western and aged society. Therefore, a novel therapeutic approach for this disease is critical, and an immunological view has been suggested as a novel strategy, because hyperlipidemia is closely associated with inflammation and immune dysfunction. In this study, the effects of an aqueous extract of Rubus occidentalis (RO) in obese mice were investigated using immunological indexes. The mice were fed a high-fat diet (HFD) to induce hyperlipidemia, which was confirmed by biochemical analysis and examination of the mouse physiology. Two different doses of RO and rosuvastatin, a cholesterol synthesis inhibitor used as a control, were orally administered. Disturbances in immune cellularity as well as lymphocyte proliferation and cytokine production were significantly normalized by oral administration of RO, which also decreased the elevated serum tumor necrosis factor $(TNF)-{\alpha}$ level and total cholesterol. The specific immune-related actions of RO comprised considerable improvement in cytotoxic T cell killing functions and regulation of antibody production to within the normal range. The immunological evidence confirms the significant cholesterol-lowering effect of RO, suggesting its potential as a novel therapeutic agent for hyperlipidemia and associated immune decline.
Modified Aloe Polysaccharide Restores Chronic Stress-Induced Immunosuppression in Mice
Lee, Youngjoo,Im, Sun-A,Kim, Jiyeon,Lee, Sungwon,Kwon, Junghak,Lee, Heetae,Kong, Hyunseok,Song, Youngcheon,Shin, Eunju,Do, Seon-Gil,Lee, Chong-Kil,Kim, Kyungjae MDPI 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.10
<P>Chronic stress generally experienced in our daily lives; is known to augment disease vulnerability by suppressing the host immune system. In the present study; the effect of modified Aloe polysaccharide (MAP) on chronic stress-induced immunosuppression was studied; this Aloe compound was characterized in our earlier study. Mice were orally administered with MAP for 24 days and exposed to electric foot shock (EFS; duration; 3 min; interval; 10 s; intensity; 2 mA) for 17 days. The stress-related immunosuppression and restorative effect of MAP were then analyzed by measuring various immunological parameters. MAP treatment alleviated lymphoid atrophy and body weight loss. The numbers of lymphocyte subsets were significantly normalized in MAP-treated mice. Oral administration of MAP also restored the proliferative activities of lymphocytes; ovalbumin (OVA)-specific T cell proliferation; antibody production; and the cell killing activity of cytotoxic T lymphocytes. In summary; oral administration of MAP ameliorated chronic EFS stress-induced immunosuppression.</P>
Molecular Characterization of Bacteriophages for Microbial Source Tracking in Korea
Lee, Jung Eun,Lim, Mi Young,Kim, Sei Yoon,Lee, Sunghee,Lee, Heetae,Oh, Hyun-Myung,Hur, Hor-Gil,Ko, GwangPyo American Society for Microbiology 2009 Applied and environmental microbiology Vol.75 No.22
<B>ABSTRACT</B><P>We investigated coliphages from various fecal sources, including humans and animals, for microbial source tracking in South Korea. Both somatic and F<SUP>+</SUP>-specific coliphages were isolated from 43 fecal samples from farms, wild animal habitats, and human wastewater plants. Somatic coliphages were more prevalent and abundant than F<SUP>+</SUP> coliphages in all of the tested fecal samples. We further characterized 311 F<SUP>+</SUP> coliphage isolates using RNase sensitivity assays, PCR and reverse transcription-PCR, and nucleic acid sequencing. Phylogenetic analyses were performed based on the partial nucleic acid sequences of 311 F<SUP>+</SUP> coliphages from various sources. F<SUP>+</SUP> RNA coliphages were most prevalent among geese (95%) and were least prevalent in cows (5%). Among the genogroups of F<SUP>+</SUP> RNA coliphages, most F<SUP>+</SUP> coliphages isolated from animal fecal sources belonged to either group I or group IV, and most from human wastewater sources were in group II or III. Some of the group I coliphages were present in both human and animal source samples. F<SUP>+</SUP> RNA coliphages isolated from various sources were divided into two main clusters. All F<SUP>+</SUP> RNA coliphages isolated from human wastewater were grouped with Qβ-like phages, while phages isolated from most animal sources were grouped with MS2-like phages. UniFrac significance statistical analyses revealed significant differences between human and animal bacteriophages. In the principal coordinate analysis (PCoA), F<SUP>+</SUP> RNA coliphages isolated from human waste were distinctively separate from those isolated from other animal sources. However, F<SUP>+</SUP> DNA coliphages were not significantly different or separate in the PCoA. These results demonstrate that proper analysis of F<SUP>+</SUP> RNA coliphages can effectively distinguish fecal sources.</P>
Effect of Metformin on Metabolic Improvement and Gut Microbiota
Lee, Heetae,Ko, GwangPyo American Society for Microbiology 2014 Applied and environmental microbiology Vol.80 No.19
<P>Metformin is commonly used as the first line of medication for the treatment of metabolic syndromes, such as obesity and type 2 diabetes (T2D). Recently, metformin-induced changes in the gut microbiota have been reported; however, the relationship between metformin treatment and the gut microbiota remains unclear. In this study, the composition of the gut microbiota was investigated using a mouse model of high-fat-diet (HFD)-induced obesity with and without metformin treatment. As expected, metformin treatment improved markers of metabolic disorders, including serum glucose levels, body weight, and total cholesterol levels. Moreover, <I>Akkermansia muciniphila</I> (12.44% ± 5.26%) and <I>Clostridium cocleatum</I> (0.10% ± 0.09%) abundances increased significantly after metformin treatment of mice on the HFD. The relative abundance of <I>A. muciniphila</I> in the fecal microbiota was also found to increase in brain heart infusion (BHI) medium supplemented with metformin <I>in vitro</I>. In addition to the changes in the microbiota associated with metformin treatment, when other influences were controlled for, a total of 18 KEGG metabolic pathways (including those for sphingolipid and fatty acid metabolism) were significantly upregulated in the gut microbiota during metformin treatment of mice on an HFD. Our results demonstrate that the gut microbiota and their metabolic pathways are influenced by metformin treatment.</P>
Lee, Youngjoo,Kim, Jiyeon,An, Jinho,Lee, Heetae,Kong, Hyunseok,Song, Youngcheon,Shin, Eunju,Do, Seon-Gil,Lee, Chong-Kil,Kim, Kyungjae Taylor & Francis 2017 Bioscience, Biotechnology, and Biochemistry Vol. No.
<P>We developed spontaneous diet-induced metabolic disease in mice by feeding them a high-fat diet for 23weeks and administered Aloe QDM complex for 16weeks to examine its restorative effect on immune disorders and metabolic syndrome. A series of immune functional assays indicated Aloe QDM complex enhanced lymphocyte proliferation and antigen-specific immunity as determined by the restored functions of cytotoxic T lymphocytes (CTL) and IgG production. The elevated serum TNF- level was also regulated by Aloe QDM complex treatment, which suggested its complex therapeutic potential. As for metabolic phenotypes, oral administration of Aloe QDM complex significantly improved diabetic symptoms, including high fasting glucose levels and glucose tolerance, and distinctly alleviated lipid accumulation in adipose and hepatic tissue. The simultaneous restoration of Aloe QDM complex on metabolic syndrome and host immune dysfunction, especially on the specific CTL killing was first elucidated in our study.</P>
Evaluation of electropositive filtration for recovering norovirus in water
Lee, Heetae,Kim, Misoon,Paik, Soon-Young,Lee, Chan Hee,Jheong, Weon-Hwa,Kim, Jongmin,Ko, GwangPyo IWA Publishing 2011 Journal of water and health Vol.9 No.1
<P>The virus adsorption-elution (VIRADEL) technique has been widely used in the recovery of various enteric viruses in water, and an electropositive filter such as 1 MDS has been commonly applied. However, effective methods of monitoring waterborne norovirus (NoV) have not yet been well characterized and optimized. Hence, in this study, the VIRADEL technique was evaluated and optimized for effectively detecting NoV in water by two commonly used electropositive filters (1MDS and NanoCeram filter). Various elution and concentration methods were evaluated by using both murine norovirus (MNV) and human NoV. Among the tested elution buffers, the most effective was 1.5% beef extract plus 0.01% Tween 80 for both 1MDS (67.5%) and NanoCeram (85.7%) microfilters. The recovery rate of GII-4 human NoV was higher by organic flocculation (86.6%) than by polyethylene glycol (PEG) precipitations (11.6~73.6%). When both 1MDS and NanoCeram filters were tested to detect NoV in surface and groundwater, the sensitivity of NoV recovered by these filters appeared to depend on the types and conditions of environmental water. The results of this study will help to set a standard of detection method for NoV in water.</P>
( Heetae Lee ),( Chong-kil Lee ),( Kyungjae Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 한국미생물·생명공학회지 Vol.49 No.1
Human breast milk is a potential source of bacteria for the development of the intestinal microbiota of infants. Several species within the genera Lactobacillus and Bifidobacterium were demonstrated to shape the gut microbiota of infants. In this study, the bacterial diversity was investigated in the breast milk and feces of a mother-infant pair, and probiotic candidates were identified. Importantly, the novel L. gasseri EJL and B. breve JTL strains were isolated from breast milk and infant feces samples, respectively; their completed genome was resolved using de novo sequencing. In addition, the bacterial composition in the infant’s feces at 1 week revealed the prevalence of Bifidobacterium and Streptococcus; a higher diversity was observed after 3 weeks. In particular, the abundance of Akkermansia was sharply increased at 7 weeks, further increasing thereafter, up to 15 weeks. Our results suggest that human breast milk and infant’s feces are a source of probiotic candidates.
IoT Smart Home Adoption: The Importance of Proper Level Automation
Yang, Heetae,Lee, Wonji,Lee, Hwansoo Hindawi Limited 2018 Journal of sensors Vol.2018 No.-
<P>The word “smart” has been used in various fields and is widely accepted to mean intelligence. Smart home service, one of the representative emerging technologies in the IoT era, has changed house equipment into being more intelligent, remote controllable, and interconnected. However, the intelligence and controllability of a smart home service are contradictory concepts, under certain aspects. In addition, the level of intelligence or controllability of a smart home service that users want may differ according to the user. As potential users of smart home services have diversified in recent years, providing the appropriate functions and features is critical to the diffusion of the service. Thus, this study examines the smart home service features that current users require and empirically evaluates the relationship between the critical factors and the adoption behavior with 216 samples from Korea. The moderating effect of personal characteristics on behavior is also tested. The results of the analysis provide various theoretical and practical implications.</P>