Designing a Non-invasive Surface Acoustic Resonator for Ultra-high Sensitive Ethanol Detection for an On-the-spot Health Monitoring System

Peyman Jahanshahi,Qin Wei,Zhang Jie,Erfan Zal Nezhad 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.4

Surface acoustic wave (SAW) sensors – based on piezoelectric crystal resonators – are extremely sensitive to even very small perturbations in the external atmosphere, because the energy associated with the acoustic waves is confined to the crystal surface. In this study, we present a critical review of the recent researches and developments predominantly used for SAW-based organic vapor sensors, especially ethanol. Besides highlighting their potential to realize real-time ethanol sensing, their drawbacks such as indirect sensing, invasive, time initializing, and low reliability, are properly discussed. The study investigates a proposed YZ-lithium niobate piezoelectric substrate with interdigital transducers patterned on the surface. Design of the resonator plays an important role in improving mass sensitivity, particularly the sensing area. Accordingly, a tin dioxide (SnO2) layer with a specific thickness is generated on the surface of the sensor because of its high affinity to ethanol molecules. To determine the values of sensor configuration without facing the practical problems and the long theoretical calculation time, it is shown that the mass sensitivity of SAW sensors can be calculated by a simple three-dimensional (3-D) finite element analysis (FEA) using a commercial finite-element platform. In design validation step, different concentrations of ethanol are applied to investigate the acoustic wave properties of the sensor. The FEA data are used to obtain the surface and bulk total displacements of the sensor and fast Fourier transform (FFT) on output spectrum. The sensor could develop into highly sensitive and fast responsive structure so that a positive intensity shift of 0.18e-2 RIU is observed when the sensor is exposed to 15 ppm ethanol. It is capable of continuously monitoring the ethanol gas whether as an ultra-high sensitive sensor or switching applications for medical and industrial purposes.

Hair plucking as an alternative non-invasive sampling method for the detection of African swine fever virus

Chae-Rin Park,Tae-Young Suh,Da-Hye Yi,이수경,Seong-Keun Hong,Ki-Hyun Cho,Jee-Yong Park,Yong-Joo Kim,Hae-Eun Kang,Jun-Gu Choi 한국예방수의학회 2022 예방수의학회지 Vol.46 No.3

African swine fever (ASF) is a hemorrhagic viral disease of pigs requiring laboratory diagnosis for confirmation. Though tissue and blood samples are considered optimal for ASF diagnosis, collection of these samples can be laborious, time-consuming, and pose a risk of contaminating the environment. Here, we suggest an alternative non-invasive sampling method, hair plucking, for ASF diagnosis. ASF virus was detected in plucked hair samples from experimentally infected pigs. Although the sensitivity was inferior to whole blood, the results suggest that hair plucking can be an alternative method that can also improve animal welfare.

Effective Method for Extraction of Cell-Free DNA from Maternal Plasma for Non-Invasive First-Trimester Fetal Gender Determination: A Preliminary Study

Lim, Ji-Hyae,Park, So-Yeon,Kim, Shin-Young,Kim, Do-Jin,Kim, Mee-Jin,Yang, Jae-Hyug,Kim, Moon-Young,Kim, Min-Hyoung,Han, Ho-Won,Choi, Kyu-Hong,Ryu, Hyun-Mee Korean Society of Medical Genetics and Genomics 2010 대한의학유전학회지 Vol.7 No.1

목 적: 모체 혈장으로부터 가장 효과적으로 세포 유리 DNA(cell free DNA, cf-DNA)를 추출하는 방법을 찾기 위해 우리는 viral DNA 추출 방법과 일반 혈액DNA 추출 방법을 이용하여 비침습적 임신 초기 태아 성별 확인 결과를 비교하였다. 대상 및 방법: 임신 초기 44명의 임산부로부터 모여진 모체 혈장을 통한 전향적 연구가 구성되었다. Cf-DNA는 viral DNA 추출 방법과 일반 혈액 DNA 추출 방법을 이용하여 각각 추출되었다. 정량 형광-중합효소 연쇄 반응(QF-PCR)을 이용하여SRY 와AMXY 유전자를 검출하였다. QF-PCR의 진단 정확도는 최종 분만 기록을 토대로 결정하였다. 결 과: 전체 44명의 여성이 실험에 참여하였지만, 최종 분만 기록은 단지 36명의 여성에서 획득하였다. 이들 중 16명은 남아를 20명은 여아를 임신하였다. 두 추출 방법에서 태아 성별의 진단적 정확도는 일반 혈액 DNA 추출 방법에 경우 63.9% (23/26)였으며 viral DNA 추출 방법에 경우 97.2% (35/36) 였다. 결 론: QF-PCR을 이용한 비침습적 임신초기 태아 성별 확인에 있어 viral kit를 사용하는 것이 높은 진단적 정확도를 이끌 수 있을 것으로 사료된다. Purpose: To find the most effective method for extraction of cell-free DNA (cf-DNA) from maternal plasma, we compared a blood DNA extraction system (blood kit) and a viral DNA extraction system (viral kit) for non-invasive first-trimester fetal gender determination. Materials and Methods: A prospective cohort study was conducted with maternal plasma collected from 44 women in the first-trimester of pregnancy. The cf-DNA was extracted from maternal plasma using a blood kit and a viral kit. Quantitative fluorescent-polymerase chain reaction (QF-PCR) was used to detect the SRY gene and AMEL gene. The diagnostic accuracy of the QF-PCR results was determined based on comparison with the final delivery records. Results: A total of 44 women were tested, but the final delivery record was only obtained in 36 cases which included 16 male-bearing and 20 female-bearing pregnancies. For the blood kit and viral kit, the diagnostic accuracies for fetal gender determination were 63.9% (23/36) and 97.2% (35/36), respectively. Conclusion: In non-invasive first-trimester fetal gender determination by QF-PCR, using a viral kit for extraction of cf-DNA may result in a higher diagnostic accuracy.

Effective Method for Extraction of Cell-Free DNA from Maternal Plasma for Non-Invasive First-Trimester Fetal Gender Determination : A Preliminary Study

Ji Hyae Lim,So Yeon Park,Shin Young Kim,Do Jin Kim,Mee Jin Kim,Jae Hyug Yang,Moon Young Kim,Min Hyoung Kim,Ho Won Han,Kyu Hong Choi,Hyun Mee Ryu 대한의학유전학회 2010 대한의학유전학회지 Vol.7 No.1

목적 : 모체 혈장으로부터 가장 효과적으로 세포 유리 DNA(cell free DNA, cf-DNA)를 추출하는 방법을 찾기 위해 우리는 viral DNA 추출 방법과 일반 혈액 DNA 추출 방법을 이용하여 비침습적 임신 초기 태아 성별 확인 결과를 비교하였다. 대상 및 방법 : 임신 초기 44명의 임산부로부터 모여진 모체혈장을 통한 전향적 연구가 구성되었다. Cf-DNA는 viral DNA 추출 방법과 일반 혈액 DNA 추출 방법을 이용하여 각각 추출되었다. 정량 형광-중합효소 연쇄 반응(QF-PCR)을 이용하여 SRY 와 AMXY 유전자를 검출하였다. QF-PCR의 진단 정확도는 최종 분만 기록을 토대로 결정하였다. 결과 : 전체 44명의 여성이 실험에 참여하였지만, 최종 분만기록은 단지 36명의 여성에서 획득하였다. 이들 중 16명은 남아를 20명은 여아를 임신하였다. 두 추출 방법에서 태아 성별의 진단적 정확도는 일반 혈액 DNA 추출 방법에 경우 63.9%(23/26)였으며 viral DNA 추출 방법에 경우 97.2% (35/36)였다. 결론 : QF-PCR을 이용한 비침습적 임신 초기 태아 성별 확인에 있어 viral kit를 사용하는 것이 높은 진단적 정확도를 이끌 수 있을 것으로 사료된다. Purpose: To find the most effective method for extraction of cell-free DNA (cf-DNA) from maternal plasma, we compared a blood DNA extraction system (blood kit) and a viral DNA extraction system(viral kit) for non-invasive first-trimester fetal gender determination. Materials and Methods: A prospective cohort study was conducted with maternal plasma collected from 44 women in the first-trimester of pregnancy. The cf-DNA was extracted from maternal plasma using a blood kit and a viral kit. Quantitative fluorescent-polymerase chain reaction (QF-PCR) was used to detect the SRY gene and AMEL gene. The diagnostic accuracy of the QF-PCR results was determined based on comparison with the final delivery records. Results: A total of 44 women were tested, but the final delivery record was only obtained in 36 cases which included 16 male-bearing and 20 female-bearing pregnancies. For the blood kit and viral kit, the diagnostic accuracies for fetal gender determination were 63.9% (23/36) and 97.2% (35/36), respectively. Conclusion: In non-invasive first-trimester fetal gender determination by QF-PCR, using a viral kit for extraction of cf-DNA may result in a higher diagnostic accuracy.

개선된 계단 배기 방법을 이용한 혈압 측정

오홍식,이종실,지영준,김인영,Oh, Hong-Sic,Lee, Jong-Shill,Chee, Young-Joon,Kim, In-Young 대한의용생체공학회 2010 의공학회지 Vol.31 No.5

In the automatic non-invasive blood pressure measurement device, the oscillometric method iswidely used. In the oscillometric method, the step-wise deflation has the advantage of the robustness for the motion artifacts than the linear deflation method. But it has the disadvantage of its longer measurement time because we need to detect two or more pulses in a certain cuff pressure step. In this study, we suggest the modified step-wise deflation method to overcome this limitation while maintaining the general concept of step-wise deflation. Using one valid pulse in each step and the deflating valve control during the diastolic period, the measurement time could be reduced. In order to verify the accuracy of the proposed algorithm, we compared the blood pressure values from the suggested method and the blood pressure values from the conventional auscultation method. The mean and standard deviation were -0.50${\pm}$5.3mmHg and 2.08${\pm}$4.75mmHg, for systolic and diastolic blood pressure respectively. The measurement time can be reduced up to the half of conventional step-wise deflation method.

광학측정 기반 신선란 선별 장비개발

김상희(S. Kim),김재협(J. H. Kim),김국철(K. C. Kim),안영훈(Y. H. Ahn) 한국정밀공학회 2018 한국정밀공학회 학술발표대회 논문집 Vol.2018 No.5월

Biogenic Volatile Compounds for Plant Disease Diagnosis and Health Improvement

Rouhallah Sharifi,류충민 한국식물병리학회 2018 Plant Pathology Journal Vol.34 No.6

Plants and microorganisms (microbes) use information from chemicals such as volatile compounds to understand their environments. Proficiency in sensing and responding to these infochemicals increases an organism’s ecological competence and ability to survive in competitive environments, particularly with regard to plant-pathogen interactions. Plants and microbes acquired the ability to sense and respond to biogenic volatiles during their evolutionary history. However, these signals can only be interpreted by humans through the use of state-of the-art technologies. Newly-developed tools allow microbe-induced plant volatiles to be detected in a rapid, precise, and non-invasive manner to diagnose plant diseases. Beside disease diagnosis, volatile compounds may also be valuable in improving crop productivity in sustainable agriculture. Bacterial volatile compounds (BVCs) have potential for use as a novel plant growth stimulant or as improver of fertilizer efficiency. BVCs can also elicit plant innate immunity against insect pests and microbial pathogens. Research is needed to expand our knowledge of BVCs and to produce BVC-based formulations that can be used practically in the field. Formulation possibilities include encapsulation and sol-gel matrices, which can be used in attract and kill formulations, chemigation, and seed priming. Exploitation of biogenic volatiles will facilitate the development of smart integrated plant management systems for disease control and productivity improvement.

지표투과레이더를 활용한 회사벽 내부의 특성 평가

유선영 ( Seon Young Yoo ),김규호 ( Gyu Ho Kim ),김두안 ( Du Ahn Kim ),김동민 ( Dong Min Kim ) 공주대학교 문화재보존과학연구소 2023 문화재과학기술 Vol.18 No.1

본 연구는 회사벽 시편을 제작하여 지표투과레이더(GPR) 탐사장비의 벽화 내부 상태평가를 진행하고 장비의 적용성을 확인하였다. 결과에서는 횡방향과 종방향 조사, 수평단면과 수직단면 모두 내부 물질 신호의 특성 정보를 구분하였다. 횡방향 조사에서 빈 공간 탐지 목적의 시편 A와 벽화 내 이물질 탐지 목적의 시편 B는 매질별 차이와 각 물질별 위치 및 두께 차이에 따른 결과가 뚜렷하게 구별된다. 종방향 조사에서 두 시편 모두 지지층으로 사용된 점토벽돌과 빈 공간 및 벽 구성재료 간 신호가 구별되어 조적 구조에서의 GPR 적용성을 확인하였다. 데이터와 실제 시편 간 차이는 연구에서 설정된 측정조건이 내부 물질에 대한 정보를 충분히 담지 못했기 때문에 발생하였다. 차이를 줄이기 위해서는 대상에 따라 스캔속도를 조정하거나 측정방향을 늘려 충분한 양의 데이터를 얻는 것이 필요하다. 따라서 상황에 따른 측정조건과 보정법을 바탕으로 GPR 탐사는 회사벽 내부 상태평가에 활용될 수 있다. This study conducted an internal condition assessment of mural paintings using Ground Penetrating Radar and verified the applicability of the equipment. It distinguished characteristic information of material signals which were embedded in mural specimens in both transverse and longitudinal scans, including horizontal and vertical sections. Specimen A is intended for detecting voids, and specimen B is intended for detecting foreign materials in mural. In the transverse scan, both specimens showed distinct results based on differences in media, material arrangement and thickness. In the longitudinal scan, both specimens revealed distinguishable signals between the bricks, voids, and mural painting components. This confirms the applicability of GPR in evaluating masonry layer of murals. Data errors between the values derived from the program and the specimens may have occurred because the measurement conditions were not sufficient to indicate the information about the internal. To reduce the errors, adjusting the scan speed and increasing the measurement directions are necessary to obtain an adequate amount of data. Therefore, GRP detection based on appropriate measurement and calibration methods can be utilized for the assessment of mural paintings.