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원형과 사각형 단면의 미세채널에서 3차원 수력학적 집속유동 분석
윤성희(Seonghee Yoon),김경훈(Kyunghoon Kim),Ksh Krishnadash Singh,김중경(Jung Kyung Kim) 한국가시화정보학회 2010 한국가시화정보학회 학술발표대회 논문집 Vol.2010 No.11
Hydrodynamic focusing technique to generate focused flow has been used for flow cytometry in microfluidic devices. However, devices with circular channel made of glass are usually difficult for three-dimensional (3D) flow visualization. We devised a new chamber made of acryl with a pulled micropipette and a rectangular microchannel made of glass. This new channel geometry enables us to visualize the 3D flow characteristics with confocal imaging technique. Weanalyzed the hydrodynamically focused flow over a practical range of flow rates, viscosities and pressure drops.
원형 모세관과 사각형 단면의 미세채널에서 3차원 수력학적 집속유동 분석
윤성희(Seonghee Yoon),김경훈(Kyunghoon Kim),김중경(Jung Kyung Kim) 한국가시화정보학회 2011 한국가시화정보학회지 Vol.9 No.2
Hydrodynamic focusing technique to generate focused flow has been used for flow cytometry in microfluidic devices. However, devices with circular capillary tubes made of glass are not suitable for flow visualization or optical signal detection because the rays of light are distorted at the curved interface. We devised a new acrylic chamber assembled with a pulled micropipette and a rectangular microchannel made of glass. This new channel geometry enabled us to visualize the three-dimensional (3D) flow characteristics with confocal imaging technique. We analyzed the 3D hydrodynamic focusing in a circular capillary tube and a rectangular microchannel over a practical range of flow rates, viscosities and pressure drops.
Formation of liposome by microfluidic flow focusing and its application in gene delivery
Rinbok Wi,Yeonsu Oh,채찬희,김도현 한국유변학회 2012 Korea-Australia rheology journal Vol.24 No.2
We report the formation of liposomes in a simple procedure using a microfluidic hydrodynamic flow focusing method for the application in gene delivery. We fabricated microfluidic device using soft lithography and polydimethylsiloxane (PDMS) molding technique. Lipid-containing stream was surrounded by aqueous stream and liposomes were formed at the lipid-water interface. Size distribution of liposomes and zeta potential of liposome dispersion were investigated under various flow rate ratio (FRR) and processing temperature. Size distributions of liposomes were measured by dynamic light scattering (DLS), and zeta potential was measured to quantify the colloidal stability. Prepared liposomes were used as a vehicle for gene delivery, and the successful expression of delivered gene was observed by fluorescent microscope.
Formation of liposome by microfluidic flow focusing and its application in gene delivery
Wi, Rin-Bok,Oh, Yeon-Su,Chae, Chan-Hee,Kim, Do-Hyun 한국유변학회 2012 Korea-Australia rheology journal Vol.24 No.2
We report the formation of liposomes in a simple procedure using a microfluidic hydrodynamic flow focusing method for the application in gene delivery. We fabricated microfluidic device using soft lithography and polydimethylsiloxane (PDMS) molding technique. Lipid-containing stream was surrounded by aqueous stream and liposomes were formed at the lipid-water interface. Size distribution of liposomes and zeta potential of liposome dispersion were investigated under various flow rate ratio (FRR) and processing temperature. Size distributions of liposomes were measured by dynamic light scattering (DLS), and zeta potential was measured to quantify the colloidal stability. Prepared liposomes were used as a vehicle for gene delivery, and the successful expression of delivered gene was observed by fluorescent microscope.
Separation of tumor cells from the peripheral blood via a novel electro hydrodynamics model
Li, Xin,Liu, Yanping,Wang, Yingcui,Zou, Caixia Techno-Press 2021 Advances in nano research Vol.10 No.6
The significant issue that has been investigated in this research due to the great clinical potential is to separate a circulating tumor cells (CTCs) from the peripheral blood and cancer treatment in advance. Nonetheless, it is difficult to detect CTCs because of the rare existence of CTCs in the middle of peripheral blood. It is found that the need of high resolution ethods is crucial because there is a similarity in size range between CTCs types such as the cells of breast cancer and the white blood cells (WBCs). This paper presents a device which can be used for tumor cells separation from the cells of blood with nonstop flow that is helped by fractionating dielectrophoresis (DEP) field-flow. The reason that leads CTCs to separate from the cells of blood is the obvious different sizes of hydrodynamics focusing and dielectrophoretic force. Numerous attempts have been made to calculate CTCs trajectories with the aid of simulating the flow speed and electric field and it reveals an accurate comparison of them with the measured results. Furthermore, the low applied voltage such 10 V<sub>pp</sub> with which the represented device can be utilized. The high precision and efficiency of particle separation can be obtained by the device as well. According to the differences in size, this approach has various application for separation of other particles sorts. Based on our findings in this study, it is assumed that our device is beneficial for studying cancer and also has an excellent capability of separating tumor cells from blood cells.