Optimization of Culture Conditions for the Production of Pleuromutilin from Pleurotus Mutilus Using a Hybrid Method Based on Central Composite Design, Neural Network, and Particle Swarm Optimization

Latifa Khaouane,Chérif Si-Moussa,Salah Hanini,Othmane Benkortbi 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.5

This study aims at optimizing the culture conditions (agitation speed, temperature and pH) of the Pleuromutilin production by Pleurotus mutilus. A hybrid methodology including a central composite design (CCD),an artificial neural network (ANN), and a particle swarm optimization algorithm (PSO) was used. Specifically, the CCD and ANN were used for conducting experiments and modeling the non-linear process, respectively. The PSO was used for two purposes: Replacing the standard back propagation in training the ANN (PSONN) and optimizing the process. In comparison to the response surface methodology (RSM) and to the Bayesian regularization neural network (BRNN), PSONN model has shown the highest modeling ability. Under this hybrid approach (PSONN-PSO), the optimum levels of culture conditions were: 242 rpm agitation speed; temperature 26.88 and pH 6.06. A production of 10,074 ± 500 μg/g, which was in very good agreement with the prediction (10,149 μg/g),was observed in verification experiment. The hybrid PSONN-PSO gave a yield of 27.5% greater than that obtained by the hybrid BRNN-PSO. This work shows that the combination of PSONN with the generic PSO algorithm has a good predictability and a good accuracy for bio-process optimization. This hybrid approach is sufficiently general and thus can be helpful for modeling and optimization of other industrial bio-processes.

배양방법과 배지성분이 시루송편버섯 균사체 매트 형성에 미치는 영향

정용현,신현재,김다송,김현석,오득실 한국버섯학회 2022 한국버섯학회지 Vol.20 No.2

Eco-friendly materials, such as alternative vegan materials using various fungal resources, are being actively researched to reduce environmental pollution and facilitate a healthy lifestyle. The fungal mycelium-based mushroom mycelium mat is one such emerging material. In this study, the commonly used mushroom mycelium culture method was modified to reduce the time required to produce the mycelium mat, lower the possibility of contamination, and improve the properties and quality of the mat. Shortening the period required for the previously used primary bag culture and secondary mat production culture. A culture method in which the bag culture was omitted was attempted using a mycelium mutated by gamma irradiation to the mycelium of Trametes orientalis. In addition, various nutrients were added to the fungal solution to observe the change in physical properties of the fungal mat. High-quality mycelium mats were produced in the experimental group containing 1.5% CaCO3 in sawdust medium, and the period was also reduced by more than 10 days compared to the existing production method. In the future, for mass producing mycelium mats, additional selection of medium components and optimization of culture conditions are essential.

Optimization for the Enhanced Production of Avermectin B1b from Streptomyces avermitilis DSM 41445 Using Artificial Neural Network

Siddique, Samia,Nelofer, Rubina,Syed, Quratulain,Adnan, Ahmad,Qureshi, Fahim Ashraf 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5

Avermectin is an environment friendly bio-insecticide. Optimization of the culture conditions for avermectin B1b production has not been carried out before using Artificial Neural Network (ANN) method. The present work is therefore conducted to optimize some important factors including yeast extract, $MgSO_4.7H_2O$, and temperature for the avermectin B1b production using ANN methodology from Streptomyces avermitilis DSM 41445. The optimum levels for the yeast extract, $MgSO_4.7H_2O$, and temperature were 16.0 (g/L), 5.0 (g/L) and $32^{\circ}C$ respectively. Maximum effect was observed by yeast extract. Avermectin B1b yield was increased up to 150% after optimization. ANN was found to be a powerful technique for the optimization and prediction of avermectin B1b production from Streptomyces avermitilis DSM 41445.

Culture of Human Chondrocytes in a Macroporous PLGA Scaffold for up to Sixteen Weeks

JeongJoonYoo,HaNaKim,EunHeeBae,HeungJaeChun,HeeJoongKim 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.3

Chondrocyte-specific phenotypic characteristics should be properly expressed and viable cells should besufficient after in vitro culture in a polymeric scaffold to repair cartilaginous defects. In this study, we culturedhuman articular chondrocytes in a macroporous PLGA scaffold for 16 weeks and monitored the phenotypic charac-teristics and viability of chondrocytes to determine the optimal culture period. After 4 weeks of culture, the numberof chondrocytes decreased, and the amount of extracellular matrix decreased as well. In addition, the scaffold matrixappeared fragmented and degraded. Immunohistochemical staining showed that the amount of type II collagenmatrix was highest at 4 weeks, and declined with time. Scanning electron microscope observations also showed atime-dependent decrease in chondrocytes and matrices after 4 weeks of culture. In addition, TUNEL stainingdemonstrated that apoptotic TUNEL-positive cells were evenly distributed in the constructs at 2 weeks of cultureand increased thereafter. Together these findings suggest that the phenotypic characteristics and viabilities of humanarticular chondrocytes in a macroporous PLGA scaffold are optimal at 4 weeks of in vitro culture. Greater than 4weeks of culture is not advantageous for chondrocytes when the macroporous PLGA scaffold is used.

Production of Tropane Alkaloids by Two-stage Culture of Scopolia parviflora Nakai Adventitious Root

Kim, Won-Jung,Jung, Hee-Young,Min, Ji-Yun,Chung, Young-Gwan,Lee, Cheol-Ho,Choi, Myung-Suk Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

Scopolia parviflora Nakai, a rare and endangered species, os the sole plant producing tropane alkaloids (TA) among the Korean native species. In order to enhance TA productivity the SP72 root, line was selected by screening 100 of root line, and the optimal culture media for root growth and TA production were investigated with the SP72 roots. Based on the several media, SH and 2B5 medium were determined as growth medium and White and NN medium as production medium. Among the four combinations of two-stage culture, 2BN (2B5 as growth medium plus NN as production medium) showed more enhanced root growth and TA production as compared with production media of White and NN medium and growth media of SH and 2B5 medium, respectively. However, bubble column bioreactor (BCB) cultures applying two-stage culture did not reveal the effective results despite of the each successful operation of two-stage culture in conical flasks and BCB cultures.

Production of Tropane Alkaloids by Two-stage Culture of Scopolia parviflora Nakai Adventitious Root

Kim, Won-Jung,Jung, Hee-Young,Min, Ji-Yun,Chung, Young-Gwan,Lee, Cheol-Ho,Choi, Myung-Suk The Korean Society of Medicinal Crop Science 2004 韓國藥用作物學會誌 Vol.12 No.5

Scopolia parviflora Nakai, a rare and endangered species, is the sole plant producing tropane alkaloids (TA) among the Korean native species. In order to enhance TA productivity the SP72 root line was selected by screening 100 of root line, and the optimal culture media for root growth and TA production were investigated with the SP72 roots. Based on the several media, SH and 2B5 medium were determined as growth medium and White and NN medium as production medium. Among the four combinations of two-stage culture, 2BN (2B5 as growth medium plus NN as production medium) showed more enhanced root growth and TA production as compared with production media of White and NN medium and growth media of SH and 2B5 medium, respectively. However, bubble column bioreactor (BCB) cultures applying two-stage culture did not reveal the effective results despite of the each successful operation of two-stage culture in conical flasks and BCB cultures.

Production of Tropane Alkaloids by Two-stage Culture of Scopolia parviflora Nakai Adventitious Root

김운정 한국약용작물학회 2004 韓國藥用作物學會誌 Vol.12 No.5

Scopolia parviflora Nakai, a rare and endangered species, is the sole plant producing tropane alkaloids (TA) among the Korean native species. In order to enhance TA productivity the SP72 root line was selected by screening 100 of root line, and the optimal culture media for root growth and TA production were investigated with SP72 roots. Based on the several media, SH and 2B5 medium were determined as growth medium and White and NN medium as production medium. Among the four combinations of two-stage culture, 2BN (2B5 as growth medium Plus NN as production medium) showed more enhanced root growth and TA production as compared with production media of White and NN medium and growth media of SH and 2B5 medium respectively. However, bubble column bioreactor (BCB) cultures applying two-stage culture did not reveal the effective results despite of the each successful operation of two-stage culture in conical flasks and BCB cultures.

Aloe vera L. Callus의 현탁배양에 의한 세포외 다당생산 및 최적화

김명욱 ( Myung Uk Kim ),조영제 ( Young Je Cho ),이신영 ( Shin Young Lee ) 한국산업식품공학회 2012 산업 식품공학 Vol.16 No.3

The extracellular polysaccharide production from suspension culture of Aloe vera L. was analyzed, and the optimization for suspension cultivation was investigated. From specific polysaccharide assay of glucomannan, FT-IR and 1H NMR spectrum, the extracellular polysacchride from suspension culture was found to be similar to β-1,4-glucomannan originated from Aloe vera fresh leaf. The polysaccharide existed in an acetylated form and its molecular weight by gel permeation chromatography was estimated to be 490 kD (Mn = 440 kD; polydispersity = 1.115). From medium optimization by simplex-cemtroid design using MINITAB®, the callus growth on the 2,4-D of 11.82 μM, kinetin of 13.84 μM and α-naphthalene acetic acid of 4.34 μM was turned out to be significantly superior than the growth on the other culture media combinations. Under this optimal culture media, other conditions such as the suspension culture``s temperature, pH and the growth terms were determined. These results showed that the callus growth was optimum at 25oC, pH 5.5 and in 2 weeks. The maximum levels of growth and extracellular polysaccharide production of callus by optimal conditions were about 20.4 and 2.5 g/L, respectively, showing the profile of extracellular polysaccharide production was closely related to the callus growth.

Production of Tropane Alkaloids by Two-stage Culture of Scopolia parviflora Nakai Adventitious Root

Won-Jung Kim,Hee-Young Jung,Ji-Yun Min,Young-Gwan Chung,Cheol-Ho Lee,Myung-Suk Choi 韓國藥用作物學會 2004 한국약용작물학회지 Vol.12 No.5

Scopolia parviflora Nakai, a rare and endangered species, is the sole plant producing tropane alkaloids (TA) among the Korean native species. In order to enhance TA productivity the SP72 root line was selected by screening 100 of root line, and the optimal culture media for root growth and TA production were investigated with the SP72 roots. Based on the several media, SH and 2B5 medium were determined as growth medium and White and NN medium as production medium. Among the four combinations of two-stage culture, 2BN (2B5 as growth medium plus NN as production medium) showed more enhanced root growth and TA production as compared with production media of White and NN medium and growth media of SH and 2B5 medium, respectively. However, bubble column bioreactor (BCB) cultures applying two-stage culture did not reveal the effective results despite of the each successful operation of two-stage culture in conical flasks and BCB cultures.

Aloe vera L. Callus의 현탁배양에 의한 세포외 다당생산 및 최적화

김명욱,조영제,이신영 한국산업식품공학회 2012 산업 식품공학 Vol.16 No.3

The extracellular polysaccharide production from suspension culture of Aloe vera L. was analyzed, and the optimization for suspension cultivation was investigated. From specific polysaccharide assay of glucomannan, FT-IR and 1H NMR spectrum, the extracellular polysacchride from suspension culture was found to be similar to β-1,4-glucomannan originated from Aloe vera fresh leaf. The polysaccharide existed in an acetylated form and its molecular weight by gel permeation chromatography was estimated to be 490 kD (Mn = 440 kD; polydispersity = 1.115). From medium optimization by simplex-cemtroid design using MINITAB®, the callus growth on the 2,4-D of 11.82 μM, kinetin of 13.84 μM and α-naphthalene acetic acid of 4.34 μM was turned out to be significantly superior than the growth on the other culture media combinations. Under this optimal culture media, other conditions such as the suspension culture's temperature, pH and the growth terms were determined. These results showed that the callus growth was optimum at 25oC, pH 5.5 and in 2 weeks. The maximum levels of growth and extracellular polysaccharide production of callus by optimal conditions were about 20.4 and 2.5 g/L, respectively, showing the profile of extracellular polysaccharide production was closely related to the callus growth.