陸地棉과 亞細亞棉의 種間交雜에서 花粉發芽, 受精 및 種子 發育

Hong Jae Park(朴洪在),Kyung Soo Min(閔庚洙),Jang Soon Ahn(安奬淳) 한국육종학회 1997 한국육종학회지 Vol.29 No.1

Histological studies were carried out to investigate a period of pollen germination, pollen tube elongation, fertilization, endosperm and embryo development and fiber formation in Gossypium hirsutum, G. arboreum and their reciprocal crosses. Pollen germination started within 30 minutes after pollination in the selfings of G. hirsutum and G. arboreum, and G. arboreum×G. hirsutum and cross, but after 1 hour in G. hirsutum×G. arboreum cross. The fertilization of the egg took 24~30 hours in G. hirsutum selfings, 28~30 hours in G. arboreum selfings, 36~44 hours in G. hirsutum×G. arboreum cross and 28~36 hours in G. arboreum×G. hirsutum cross. Proembryos appeared 5 days after pollination in the selfings and embryos started to abort 10 days after pollination in the interspecific crossing. In the testa fiber, fiber cell began to grow in 24~48 hours after pollination, length of a matured fiber was 22~32mm and 19~22mm in Gossypium hirsutum and G. arboreum, respectively. The development of testa fiber was stopped to grow 7~10 days after pollination in Gossypium hirsutum×G. arboreum, and the length of fiber was 10~13mm. The ovule and embryo culture to obtain the interspecific hybrid plants between Gossypium hirsutum and G. arboreum should start 2 to 6 days after pollination, when endosperm degeneration initiates.

陸地棉, 亞細亞棉 및 種間雜種의 胚珠培養

Hong Jae Park(朴洪在),Kyung Soo Min(閔庚洙),Yong Bee Oh(吳龍飛),Dong Hee Chung(鄭東熙) 한국육종학회 1996 한국육종학회지 Vol.28 No.4

This experiments were conducted to establish ovule culture method for Gossypium hirsutum, G. arboreum and their hybrids to overcome difficulty to interspecific hybridization due to the ovule abortion in artificial crosses. In the ovule culture of Gossypium hirsutum and G. arboreum the percentage of survival ovule and callus formation in four weeks after culture was 45.6% in G. hirsutum and 37.3% in G. arboreum using MS medium including casein hydrolysate 250㎎/ℓ, IAA 5.0μM and kinetin 0.2μM concentration. The Schenk-Hildebrandt medium was superior to Beasley-Ting or Murashige-Skook ones for ovule culture of interspecific hybrid. Schenck-Hildebrandt medium added by LAA 5μM, Kinetin 0.2μM concentration showed the highest in the rate of survival embryo and plant formation with 44.4% and 2.9% in G. hirsutum×G. arboreum hybrids and 20.1%, 1.5% in the reciprocal hybrids, respectively. Both cotton species showed higher embryo survival and shoot formation in ovule culture (SH medium, 28℃) under the first fifteen days dark and then 16 hours condition light/8 hours dark conditions compared with continuous dark or light treatments. The hybrid ovules dissected out at four days after interspecific pollinations revealed high embryo survival and shoot formation.

Diversity in Betasatellites Associated with Cotton Leaf Curl Disease During Source-To-Sink Movement Through a Resistant Host

Iftikhar Ali Khan,Khalid Pervaiz Akhtar,Fazal Akbar,Ishtiaq Hassan,Imran Amin,Muhammad Saeed,Shahid Mansoor 한국식물병리학회 2016 Plant Pathology Journal Vol.32 No.1

Cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. The disease symptoms are induced by DNA satellite known as Cotton leaf curl Multan betasatellite (CLCuMuB), dominant betasatellite in cotton but another betasatellite known as Chili leaf curl betasatellite (ChLCB) is also found associated with the disease. Grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cotton (several distinct strains of CLCuMuB are associated with the disease). Infected scion of Gossypium hirsutum collected from field (the source) was grafted on G. arboreum, a diploid cotton species, resistant to the disease. A healthy scion of G. hirsutum (sink) was grafted at the top of G. arboreum to determine the movement of virus/betasatellite to upper susceptible scion of G. hirsutum. Symptoms of disease appeared in the upper scion and presence of virus/betasatellite in the upper scion was confirmed via molecular techniques, showing that virus/betasatellite was able to move to upper scion through resistant G. arboreum. However, no symptoms appeared on G. arboreum. Betasatelites were cloned and sequenced from lower scion, upper scion and G. arboreum which show that the lower scion contained both CLCuMuB and ChLCB, however only ChLCB was found in G. arboreum. The upper scion contained CLCuMuB with a deletion of 78 nucleotides (nt) in the non-coding region between Arich sequence and βC1 gene and insertion of 27 nt in the middle of βC1 ORF. This study may help in investigating molecular basis of resistance in G. arboreum.

Diversity in Betasatellites Associated with Cotton Leaf Curl Disease During Source-To-Sink Movement Through a Resistant Host

Khan, Iftikhar Ali,Akhtar, Khalid Pervaiz,Akbar, Fazal,Hassan, Ishtiaq,Amin, Imran,Saeed, Muhammad,Mansoor, Shahid The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.1

Cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. The disease symptoms are induced by DNA satellite known as Cotton leaf curl Multan betasatellite (CLCuMuB), dominant betasatellite in cotton but another betasatellite known as Chili leaf curl betasatellite (ChLCB) is also found associated with the disease. Grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cotton (several distinct strains of CLCuMuB are associated with the disease). Infected scion of Gossypium hirsutum collected from field (the source) was grafted on G. arboreum, a diploid cotton species, resistant to the disease. A healthy scion of G. hirsutum (sink) was grafted at the top of G. arboreum to determine the movement of virus/betasatellite to upper susceptible scion of G. hirsutum. Symptoms of disease appeared in the upper scion and presence of virus/betasatellite in the upper scion was confirmed via molecular techniques, showing that virus/betasatellite was able to move to upper scion through resistant G. arboreum. However, no symptoms appeared on G. arboreum. Betasatelites were cloned and sequenced from lower scion, upper scion and G. arboreum which show that the lower scion contained both CLCuMuB and ChLCB, however only ChLCB was found in G. arboreum. The upper scion contained CLCuMuB with a deletion of 78 nucleotides (nt) in the non-coding region between Arich sequence and ${\beta}C1$ gene and insertion of 27 nt in the middle of ${\beta}C1$ ORF. This study may help in investigating molecular basis of resistance in G. arboreum.

목화 陸地棉과 亞細亞棉 種間雜種의 胚珠培養時 生長調節劑와 炭素源의 影響

Kyung Soo Min(閔庚洙),Hong Jae Park(朴洪在) 한국육종학회 1997 한국육종학회지 Vol.29 No.3

This study was performed to increase the efficiency of interspecific hybrid production between Gossypium hirsutum and Gossypium arboreum via in vitro ovule culture. Immature ovules dissected from the interspecific crossed cotton fruit at 4 days after pollination were cultured in Shenck-Hilderbrandt(SH) liquid medium supplemented with various growth regulators and carbon sources. The best results were observed from the combination of 5mM IAA, 0.005mM GA, 0.05mM Kinetin with 18.5% of embryo survival and 10.6% plant regeneration. Glucose and sucrose were considered as reasonable carbon sources for the ovule culture of cotton achieving the highest frequency of plant regeneration at 150mM and/or 200mM, respectively.

약용식물 유래 정유성분 분석 및 산화 스트레스로부터 PC12 신경세포 보호 효과

이지연,박정용,김동휘,최수지,장귀영,서경혜,Lee, Ji Yeon,Park, Jeong-Yong,Kim, Dong Hwi,Choi, Su Ji,Jang, Gwi Young,Seo, Kyung Hye 한국식품영양학회 2020 韓國食品營養學會誌 Vol.33 No.2

The purpose of this study was to investigate the protective effect on oxidative stress induced PC12 cells, and volatile flavor composition of essential oils derived from medicinal plant seeds- Gossypium hirsutum L. (G. hirsutum), Coix lachryma-jobi (C. lachryma-jobi) and Oenothera biennis (O. biennis). The essential oils were obtained by the solvent (hexane) extraction method from the seeds. The essential oils of the seeds were analyzed by the solid-phase micro-extraction gas chromatography mass spectrometry (SPME-GC/MS). The major compounds of G. hirsutum, C. lachryma-jobi and O. biennis were cyclonexanol (16.65%), β-asarone (14.29%) and ylangene (50.01%). The DPPH radical scavenging activity (IC₅₀) was the highest value of 8.52 mg/mL in the O. biennis. Additionally, IC₅₀ values of G. hirsutum and C. lachryma-jobi were 26.76 mg/mL and 36.81 mg/mL. For the oxidative stress on PC12 cells, we treated with hydrogen peroxide (H₂O₂). The pretreatment of oxidative stress induced PC12 cells with all the essential oils preserved or increased their cell viability and G. hirsutum and O. biennis attenuated the ROS generation (by 68.75% and 56.25% vs. H₂O₂ control). The results of this study suggest that the essential oils derived from medicinal plant seeds could be used as valuable back data as a natural essential oil material to prevent neurodegenerative diseases by protecting neuro-cells.

Cloning and expression of glutathione S-transferase (GST) cDNA from Gossypium hirsutum L.

Kang, Won-Hee,Kim, Myong-Jo,Lim, Jung-Dae,Yun, Song-Joong,Chung, Ill-Min,Yu, Chang-Yeon The Korean Society of Medicinal Crop Science 2002 韓國藥用作物學會誌 Vol.10 No.4

A gene coding for the GST of cotton (Gh-5) was cloned into Escherichia coli and experssed. The enzyme remained within the cytoplasm of E. coli. An 696 bp open reading frame was in the 988 base pair fragment of the recombinant plasmid pET-30b(+). The deduced protein sequence consists of 232 amino acids and has a molecular mass of 30235.58 Da. The cloned enzyme conjugated reduced glutathione and 1-chloro-2,4-dinitrobenzene (CDNB). Plant GST cDNA was expressed in microbe and produced polypeptide had function as an enzyme.

The genes involved in the protective effects of phytohormones in response to Verticillium dahliae infection in Gossypium hirsutum

Huachong Zhang,Wenwei Zhang,Guiliang Jian,Fangjun Qi,Ning Si 한국식물학회 2016 Journal of Plant Biology Vol.59 No.2

Verticillium wilt disease caused by soil-borne fungus Verticillium dahliae (V. dahliae), is a serious threats to cotton growth worldwide. Endogenous phytohormones, such as salicylic acid (SA), jasmonic acid (JA), ethylene (ET), and brassinosteroid (BR) have been reported to play important roles in defense response in plants. Based on highthroughput RNA sequencing analysis of resistant Gossypium hirsutum (G. hirsutum) variety Zhongzhimian KV-3, comparing the transcriptome with V. dahliae infection and non-infection, eight differentially expressed candidate genes functions indicated in SA (GhPUB17, GhTGA7 and GhPR1), JA (GhJAZ10 and GhbHLH18), ET (GhEBF1), cytokinine (GhE13L13) and BR (GhBZR1) signal pathways were investigated via virus-induced gene silencing (VIGS) using cotton leaf crumple virus (CLCrV) vector. The expression of eight genes was further validated through quantitative realtime PCR. The result showed that the expression level of GhPUB17, GhJAZ10, GhbHLH18, GhEBF1 and GhE13L13 increased faster in resistant variety Zhongzhimian KV-3 than in susceptible variety 86-1 upon V. dahliae infection. Silencing up-regulated genes GhPUB17, GhJAZ10, GhbHLH18 and GhEBF1 significantly enhanced resistant variety susceptibility to V. dahliae and silencing down-regulated genes GhTGA7 and GhBZR1 significantly increased susceptible variety resistance to V. dahliae. Taken together, these genes are important components in response to V. dahliae infection.

Adsorption of basic red 9 on activated waste Gossypium hirsutum seeds: Process modeling, analysis and optimization using statistical design

N. Sivarajasekar,R. Baskar 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5

Sulfuric acid activated immature Gossypium hirsutum seed was utilized as adsorbent for the batch adsorption of basic red 9. The main and interactive effects of five process factors like, adsorbent dose, initial dye concentration, contact time, pH and temperature were investigated to select the influencing key factors via 25 two-level full factorial design. Box–Behnken statistical design with the selected key factors employed for process optimization. The simultaneous optimization by Derringer’s desirability function indicated that 54.27% removal of BR9 could be possible at the optimal conditions. Isotherm and kinetic studies confirmed the chemisorption on homogeneous and heterogeneous patches. 2013 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V.

陸地棉과 亞細亞棉의 種間交配時 生長調節劑 處理가 着삭에 미치는 影響

Hong Jae Park(朴洪在),Kyung Soo Min(閔庚洙),Dong Hee Chung(鄭東熙) 한국육종학회 1996 한국육종학회지 Vol.28 No.3

The present cotton cultivars were bred mostly for spinning. Accordingly their fibers are not satisfactory in elasticity, strength and water absorption, which are very important properties when they are used as padding or sanitary purposes. Interspecific hybridization was carried out with a hope to incorporate the high yield potential and early maturity of upland cottons, Gossypium hirsutum, with the highly elastic and strong fibers and excellent water absorption of Asiatic cottons, G. arboreum. Various methods such as application of growth regulators to the pollinated flowers, pollination on cut stems, pasting the stigma with pollen germination media, in vitro culture of hybrid ovules and doubling the chromosomes of the F₁ hybrid plants were attempted to overcome the interspecific barriers. An aqueous solution of 100㎎/ℓ NAA and 50㎎/ℓ GA₃ applied to the interspecifically pollinated flowers and then substantially delayed the fruit drops as shown by 49.5% bolls retained in 20 days after pollination compared to 10.9% in the control. Number of seeds harvested per interspecific cross was 0.017 on G. hirsutum and 0.008 on G. arboreum, even through all of them were empty and failed to germinate.