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결핵균 감염에 따른 큰 포식세포의 Ferritin H chain 유전자 발현
송호연,고광균 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3
The purpose of this study was to identify macrophage genes regulated by infection of mycobacteria. Mycobacterium tuberculosis H37Rv (ATCC 27294) was provided from Korean National Tuberculosis Association. We have used a differential display RT-PCR to isolated cDNAs corresponding to transcripts that induced in THP-1 cells infected with M. tuberculosis. Among several differentially expressed trascripts, one clone, designated as 1-TC8 was 322 bp and was identical to human ferritin heavy(H) chain gene. Northern blot analysis confiremed that ferritin H chain gene has been markedly over-expressed in monocytic THP-1 cells infected with live Mycobacterium tuberculosis.
Introduction of Two Types of Human Ferritin Gene into Lettuce Plants
Zu An Lee,Hae Yeong Kim,Kyu Hwan Chung,Young Doo Park 한국원예학회 2004 Horticulture, Environment, and Biotechnology Vol.45 No.6
Human H- and L-chain ferritin genes were introduced into lettuce (Lactuca sativa L.) plants by Agrobcterium-mediated transformation. These two human ferritin genes were cloned into plant binary vectors, pILTAB 357 and pCAMBIA 1302, respectively. After infection with A. tumefaciens LBA4404 containing pILTAB 357 or pCAMBIA 1302, transformed shoots were induced from selection media and regenerated shoots were rooted in rooting media. To confirm the transfer of human H- or L-chain ferritin genes in the genome of lettuce plants, polymerase chain reaction (PCR) was conducted using specific primers designed from the H- and L- chain ferritin genes, respectively. The PCR fragments of 600 or 550 bp were obtained separately from the transformed plants, which are the predicted size of the H- and L- chain ferritin genes, but not from non-transformed plants. Reverse transcription-PCR showed the transcription of transgene from the transgenic plants. Inductively coupled plasma analysis for determination of Fe concentration showed that several transgenic lettuce lines with ferritin genes had higher concentration than non-tranformed ones.
Development of Double-Transformed Lettuce Plants with Two Types of Human Ferritin Gene
Zu An Lee,Young Doo Park 한국원예학회 2005 Horticulture, Environment, and Biotechnology Vol.46 No.1
Double-transformed lettuce plants were obtained from single-transformed lettuce plants with FHV vector (pILTAB 357 containing full length of human H-chain ferritin and nptII genes). Leaf disks from the singletransformed lettuce were transformed serially with FLV vector (pCAMBIA 1302 containing full length of human L-chain ferritin and hpt genes) and selected onto medium containing 50 ㎎ㆍL?¹ kanamycin, 5 ㎎ㆍL?¹ hygromycin, and 200 ㎎ㆍL?¹ cefotaxim. Green shoots were rooted in root-induction medium and regenerated plants were grown in a greenhouse. Polymerase chain reaction (PCR) analysis revealed the presence of the expected 600 and 530 bp products for human H-chain and L-chain ferritin genes, respectively. Reverse transcription-PCR analysis showed that the two types of ferritin genes could be expressed in the double-transformed plants. Most double-transformed lettuce plants except HL10 and HL12 had higher plant height differences than non-transformed plants. HL2, HL8, and HL9 were two times higher Fe concentration than the non-transformed plants, but HL5 and HL23 showed half of Fe concentration than the non-transformed plants. In this study, the two types of human ferritin genes did not have cooperative roles in Fe uptake mechanism in lettuce plants.