The purpose of this study was to identify macrophage genes regulated by infection of mycobacteria. Mycobacterium tuberculosis H37Rv (ATCC 27294) was provided from Korean National Tuberculosis Association. We have used a differential display RT-PCR to ...
The purpose of this study was to identify macrophage genes regulated by infection of mycobacteria. Mycobacterium tuberculosis H37Rv (ATCC 27294) was provided from Korean National Tuberculosis Association. We have used a differential display RT-PCR to isolated cDNAs corresponding to transcripts that induced in THP-1 cells infected with M. tuberculosis. Among several differentially expressed trascripts, one clone, designated as 1-TC8 was 322 bp and was identical to human ferritin heavy(H) chain gene. Northern blot analysis confiremed that ferritin H chain gene has been markedly over-expressed in monocytic THP-1 cells infected with live Mycobacterium tuberculosis.