돼지에 있어서 수정란의 외과적 채란 및 이식에 관한 연구

김희석,소율 기언,소도 민지,상마 정 濟州大學校 農科大學 動物科學硏究所 1993 動物科學論叢 Vol.8 No.1

Embryos collected on day 5 were best in the recovery rate and number of embryos. The number of embryos collected was most in 1 to 2 surgery times but the recovery rate of it was slightly lower. The conception rate and litter size were high when the embryos were transfered on day 4 after estrus and when the number of ovulation was 16 to 20 . While the litter size was more when the donors came into heat 1 day ealier than 2 days ealier and when the embryos were collected on day 6 than when collected on day 5, conception rates were higher when the donors came into heat 2 days ealier than when came into heat 1 day ealier and when the embryos were collected on day 5 than when collected on day 6.

돼지에 있어서 자궁각 단축술에 의한 수정란의 비외과적 채란에 관한 연구 I. 자궁각 단축이 난소, 자궁각 길이와 수정란의 회수에 미치는 영향

김희석,정종태 한국임상수의학회 1997 한국임상수의학회지 Vol.14 No.2

This study was carried out to determine the effect of uterus shortening on the pvaries, the length of uterine horn and the recovery of embryos. The length of the shortened uterine horns increased more in part of uterine tip from connecting part for shortening than in base (P<0.05), and collection of embryos was also difficult in gilts because of its narrow pelvis. The embryos collected surgically from gilts with shortened uterine horns were developed into 2~8cells (87.5%) 3days and 4cell~morula (88.9%) 5days after mating.

Embryo Collection, Transfer and Pregnancy of Riding Horses : First Successful Case in Korea

Park, Yong-Soo,Yang, Jae-Hyuk,Cho, Young-Jae,Oh, Dong-Yep,Cho, Gil-Jae The Korean Society of Embryo Transfer 2017 한국동물생명공학회지 Vol.32 No.2

Embryo transfer (ET) could be a relevant tool for genetic improvement programs in horses similar to those already underway in other species and produce multiple foals from the same mare in one breeding season. However, there have been no reports describing equine embryo transfer performed in Korea. In the present study, we performed an equine embryo collection and transfer procedure for the first time. We examined the embryo collection and pregnancy, size of embryo during the incubation period after collection, and progesterone (P4) and estradiol-$17{\beta}$ (E2) concentrations in mare's serum at embryo collection and transfer. A total of 16 donors responded to estrus synchronization; estrus was induced in 12 donors and 4 recipients, and artificial insemination was successful in 10 donors and six blastocysts were collected from donors. Of these blastocysts, we monitored the size of blastocysts for 3 day during incubation and transferred 2 blastocysts to a recipient, with 1 successful pregnancy and foal achieved. The dimensions of equine embryo at day 7 to day 9 were $409{\mu}m$, $814{\mu}m$ and $1,200{\mu}m$. The serum P4 and E2 concentrations were $7.91{\pm}0.37ng/{\mu}L$ and $45.45{\pm}12.65ng/{\mu}L$ in the donor mare, and 1$6.06{\pm}3.27ng/{\mu}L$ and $49.13{\pm}10.09ng/{\mu}L$ in the recipient mare.

Embryo Collection, Transfer and Pregnancy of Riding Horses : First Successful Case in Korea

Yong-Soo Park,Jae-Hyuk Yang,Young-Jae Cho,Dong-Yep Oh,Gil-Jae Cho 한국수정란이식학회 2017 한국동물생명공학회지 Vol.32 No.2

Embryo transfer (ET) could be a relevant tool for genetic improvement programs in horses similar to those already underway in other species and produce multiple foals from the same mare in one breeding season. However, there have been no reports describing equine embryo transfer performed in Korea. In the present study, we performed an equine embryo collection and transfer procedure for the first time. We examined the embryo collection and pregnancy, size of embryo during the incubation period after collection, and progesterone (P4) and estradiol-17ß (E2) concentrations in mare’s serum at embryo collection and transfer. A total of 16 donors responded to estrus synchronization; estrus was induced in 12 donors and 4 recipients, and artificial insemination was successful in 10 donors and six blastocysts were collected from donors. Of these blastocysts, we monitored the size of blastocysts for 3 day during incubation and transferred 2 blastocysts to a recipient, with 1 successful pregnancy and foal achieved. The dimensions of equine embryo at day 7 to day 9 were 409 μm, 814 μm and 1,200 μm. The serum P4 and E2 concentrations were 7.91±0.37 ng/μL and 45.45±12.65 ng/μL in the donor mare, and 16.06±3.27 ng/μL and 49.13±10.09 ng/μL in the recipient mare.

Embryo collection, transfer and pregnancy of riding horses : First successful case in Korea

Yong-Soo Park,Ho-Jun Lee 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10

Embryo transfer (ET) has been applied to many species, which also useful tools for genetic improvement in horses. ET enables to produce a multiple foals from one donor mare in the same breeding season. However, there have been no reports describing equine embryo transfer performed in Korea. In the present study, we performed an equine embryo collection and transfer procedure for the first time. We examined the embryo collection and pregnancy, size of embryo during the incubation period after collection, and progesterone (P4) and estradiol-17ß (E2) concentrations in mare’s serum at embryo collection and transfer. A total of 16 donors responded to estrus synchronization; estrus was induced in 12 donors and 4 recipients, and artificial insemination was successful in 10 donors and six blastocysts were collected from donors. Of these blastocysts, we monitored the size of blastocysts for 3 day during incubation and transferred 2 blastocysts to a recipient, with 1 successful pregnancy and foal achieved. The dimensions of equine embryo at day 7 to day 9 were 409 μm, 814 μm and 1,200 μm. The serum P4 and E2 concentrations were 7.91±0.37 ng/μL and 45.45±12.65 ng/μL in the donor mare, and 16.06±3.27 ng/μL and 49.13±10.09 ng/μL in the recipient mare.

Embryo Collection, Transfer and Pregnancy of Riding Horses : First Successful Case in Korea

박용수,양재혁,조영재,오동엽,조길재 사단법인 한국동물생명공학회 2017 한국동물생명공학회지 Vol.32 No.2

Embryo transfer (ET) could be a relevant tool for genetic improvement programs in horses similar to those already underway in other species and produce multiple foals from the same mare in one breeding season. However, there have been no reports describing equine embryo transfer performed in Korea. In the present study, we performed an equine embryo collection and transfer procedure for the first time. We examined the embryo collection and pregnancy, size of embryo during the incubation period after collection, and progesterone (P4) and estradiol-17ß (E2) concentrations in mare’s serum at embryo collection and transfer. A total of 16 donors responded to estrus synchronization; estrus was induced in 12 donors and 4 recipients, and artificial insemination was successful in 10 donors and six blastocysts were collected from donors. Of these blastocysts, we monitored the size of blastocysts for 3 day during incubation and transferred 2 blastocysts to a recipient, with 1 successful pregnancy and foal achieved. The dimensions of equine embryo at day 7 to day 9 were 409 μm, 814 μm and 1,200 μm. The serum P4 and E2 concentrations were 7.91±0.37 ng/μL and 45.45±12.65 ng/μL in the donor mare, and 16.06±3.27 ng/μL and 49.13±10.09 ng/μL in the recipient mare

Relationship between initiation of embryogenic suspensor mass (ESM) and zygotic embryo development in Pinus densiflora

김용욱,문흥규 한국산림과학회 2014 Forest Science And Technology Vol.10 No.3

To initiate embryogenic suspensor mass (ESM) in Pinus densiflora, immature seeds were collected on dates from 31 Mayto 20 July in 2004, 2005 and 2006, and their embryo developmental stages were checked. In materials from 2004, thehighest frequency (0.88%) of ESM initiation was obtained with explants collected on 28 June, and the seeds containedzygotic embryos in the proembryo stage (100%). The highest ESM initiation frequencies were 0.88% (28 June 2004,Suwon), 1.4% (1 July 2005, Suwon), 2.31% (1 July 2005, Anmyeondo) and 0.91% (1 July 2006, Suwon) and all embryoswere at the proembryo stage, regardless of seed collection year (2004, 2005 or 2006) or collection location (Suwon orAnmyeondo). Histological analysis from zygotic embryos (proembryos) of seeds, which were harvested at various times orlocations, revealed no significant morphological differences at various developmental stages. These results show that seedcollection time could be critical for obtaining the higher rate of ESM initiation.

황우석 사태에서의 윤리적 쟁점의 변화 : 배아윤리에서 난자윤리로

최은경(Choi Eun Kyung),김옥주(Kim Ock-Joo) 한국생명윤리학회 2006 생명윤리 Vol.7 No.2

2005년 겨울에 몰아쳤던 ‘황우석 사태’는 과학, 언론, 생명윤리, 정부, 여성 등 각계 각층에서 첨예하게 진행된 사태였다. 돌이켜보면, 황우석의 연구가 가능했던 것은 아무런 규제 없이 방치되었던 불임 시술과 난자 시장이 어려운 난자 채취 과정에도 불구하고 단 시간 내에 가장 많은 수의 난자를 취득할 수 있었기 때문이었다. 황우석 사태 이전부터 논의된 배아의 도덕적 지위의 논쟁과 인간복제배아줄기세포를 원천적으로 허용해야 할 것인 가의 문제는 여전히 남는다. 그러나 황우석 사태를 겪은 이후에는, 윤리적 쟁점이 배아의 도덕적 지위에 관한 논쟁에서 난자를 둘러싼 윤리로 핵심이 옮겨지게 되었다. 심지어 체세포 복제배아연구이 연구의 허용을 주장하는 그룹조차도, 여성의 건강과 난자 제공을 둘러싼 윤리 문제를 제고하여 새로운 대안을 내놓지 않고는 연구 허용 이야기를 꺼낼 수조차 없게 된 것이다. 황우석 연구에서 다량의 난자 채취는 여성 몸의 도구화와 난자의 상품화에 대한 문제, 난자 기증의 자발성의 문제, 충분한 설명에 근거한 동의의 문제, 불임 시술에서 환자-의사 관계와 피험자-연구자 관계의 갈등의 문제 등 해결하기 쉽지 않은 문제들을 제기하였다. 황우석 사태가 가져온 난자 관련 윤리의 문제는 비단 국내에만 국한된 것이 아니다. 체세포복제배아줄기세포연구를 진작하고자 하는 여러 국가에서도 ‘난자 수급’과 관련된 법적ㆍ사회적ㆍ윤리적 쟁점들과 불가피하게 마주해야 하는 처지에 놓이게 되었다. 우리나라는 물론 국제 사회의 다른 나라에서도 앞으로 체세포 복제 연구에 있어서 난자 기증 및 난자 매매 규제 방안, 난자제공자 관련 연구윤리 문제 등 이 사태가 낳은 여성과 난자를 둘러싼 법적ㆍ사회적ㆍ윤리적 쟁점들은 쉬이 해결되지는 않을 것이다. 오히려 향후 생명공학기술의 발전으로 인해 난자 및 여성의 재생산 관련 문제는 끊임없이 현안으로 대두될 가능성이 현저하다. 본 논문에서는 황우석 사태를 거치면서 어떻게 윤리적 쟁점이 여성의 건강과 관련된 ‘난자 윤리’로 옮겨지게 되었는가, ‘연구를 위한 난자 기증’이라는 초유의 역사적 경험이 제기한 난자를 둘러싼 윤리 문제는 무엇인가, 이러한 난자 윤리에 대해 국제적으로는 어떻게 논쟁이 진행되고 있는가에 관하여 살펴볼 것이다. 향후 배아줄기세포 연구를 둘러싼 쟁점들은 배아의 도덕적 지위를 둘러싼 논의를 넘어 난자 채취의 정당성, 여성의 위험 노출로 확대되고 있으며, 이는 배아줄기세포 연구가 국제적으로 확대될수록 더 윤리적 논란이 가속화 될 것이다. 한국에서 황우석 연구가 숱하게 낳은 쟁점들은 국제적으로도 새로운 윤리적 딜레마들을 제공한 셈이다. 배아줄기세포연구와 난자를 둘러싼 윤리적인 문제는 국제적인 협력 및 연대 속에서 논의할 필요가 점점 커지고 있다. In 2005, the whole society in South Korea fell into hot debates caused by Hwang’s scandal. The first ethical issue raised by the Hang Woo Suck's cloning research was the moral status of embryo and permissibility of human cloning. As the debates went on, the ethical debates shift to ethical problems of egg-extraction from women's ovary solely for research purposes. In retrospect, Hwang's research that used thousands of human eggs became possible because of unregulated IVF practice and black markets for human eggs in Korea. Even those who argue for permission of cloned embryo stem cell research cannot say anything without proposing appropriate safeguards against risks or harm to women’s health. After all, a tremendous number of eggs collected for Hwang's research raised important ethical issues including: commodification of eggs, exploitation of women's body, voluntariness of donation, validity of informed consent, conflict of interests of IVF doctors-stem cell investigators when they play dual roles, and many more. This paper analyzed how the shift of ethical debates from embryo ethics toward egg ethics occurred, and what the main issues were. Ethical issues on eggs collections for research brought by Hwang’s scandal do matters not only to Korea, but also to other countries that promotes somatic cell nuclear transfer embryonic stem cell research. ethical, These countries are also to face legal, social problems concerning eggs donation or collections as long as the research necessitates human eggs. As embryonic stem cell research goes further internationally, ethical debates surrounding collection of eggs for research need to be discussed and the ethical issues to be scrutinized with international collaboration.

Embryo collection, transfer and pregnancy of riding horses : First successful case in Korea

Yong-Soo, Park,Ho-Jun, Lee 한국동물생명공학회(구 한국동물번식학회) 2016 발생공학 국제심포지엄 및 학술대회 Vol.2016 No.10

돼지에 있어서 자궁각 단축술에 의한 수정란의 비외과적 채란에 관한 연구 II. 자궁각 단축이 발정주기 및 혈청 중 호르몬 수준변화에 미치는 영향

김희석 한국임상수의학회 1998 한국임상수의학회지 Vol.15 No.1

This study was carried out to determine the effects of uterus shortening on the duration required for estrus, the number of ovulation and the level of serum progesterone and prostaglandin $F_{2}{\alpha} (PGF_{2}{\alpha} $). The duration required for estrus after the surgical shortening of uterine horns and the interval between the following estrus was not affected by the surgical treatment but affected by luteal and follicular phase. The number of ovulations were increased by induction of superovulation to gilts with shortened uterine horns compared to the control. Serum progesterone concentration during the luteal phase was higher than that during the follicular phase with no difference between the control and me horns than that of the control. Findings of this study indicate that luteal formation and regressions and estrus cycle were normal when the unconnected parts of uterine horns were left in abdominal cavity. Therefore surgical shortening of uterine horns of sows helps embryo collections by non-surgical methods.