Prickly Palm Cactus Husk as a Raw Material for Production of Ligninolytic Enzymes by Aspergillus niger

Tamires Carvalho dos Santos,Nadabe dos Santos Reis,Tatielle Pereira Silva,Fabiano de Paula Pereira Machado,Renata Cristina Ferereira Bonomo,Marcelo Franco 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.1

Prickly palm cactus husk was used as a solid-state fermentation support-substrate for production of the ligninolytic enzymes laccase, peroxide manganese, and lignin peroxidase by Aspergillus niger. Effects of water activity, temperature, and fermentation time on enzymatic production were evaluated using a central composite rotatable design. Response surface methodology revealed that maximum enzyme production was achieved at 73.38 h of fermentation, a water activity of 0.87 Aw, at 28.74oC for laccase, at 65.33 h, 0.89 Aw, and 28.96oC for lignin peroxidase, and at 70.44 h, 0.91 Aw, and 28.84oC for manganese peroxidase. Optimized enzyme production was 9,023.67 UI/L for laccase, 2,234.75 UI/L for lignin peroxidase, and 8,534.81 UI/L for manganese peroxidase. Thermostability and pH stability were observed for all enzymes. Enzymatic deactivation kinetic experiments indicated that enzymes remained active after freezing of crude extracts.

Activated carbon from sewage sludge for removal of sodium diclofenac and nimesulide from aqueous solutions

Glaydson Simões dos Reis,Mohammad Khalid Bin Mahbub,Michaela Wilhelm,Eder Claudio Lima,Carlos Hoffmann Sampaio,Caroline Saucier,Silvio Luis Pereira Dias 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.11

Sludge based activated carbons (ACs) were used to remove selected pharmaceuticals such as diclofenac (DCF) and nimesulide (NM) from aqueous solutions. The powered sewage sludge was mixed with different proportions of ZnCl2. The mixture was pyrolyzed in a conventional oven using three different temperatures under inert atmosphere. Afterwards, in order to increase the specific surface area and uptake capacity the carbonized materials were acidified with 6mol L−1 HCl under reflux at 80 oC for 3 hours. The characterization of ACs was achieved by scanning electron microscopy, FTIR, TGA, hydrophobicity index by water, n-heptane vapor adsorption and nitrogen adsorption/ desorption curves. The specific surface area (SBET) of adsorbents varied between 21.2 and 679.3m2g−1. According to the water and n-heptane analysis data all ACs had hydrophobic surface. Experimental variables such as pH, mass of adsorbent and temperature on the adsorption capacities were studied. The optimum pH, mass of adsorbent and temperature for adsorption of DCF and NM onto ACs were found to be 7.0 (DCF) and 10.0 (NM), 30mg and 25 oC, respectively. The kinetic adsorption was investigated using general-order, pseudo-first order and pseudo-second order kinetic models, while the general-order model described the adsorption process most suitably. The maximum amounts of DCF and NM adsorbed were 156.7 and 66.4mg g−1 for sample 1(500-15-0.5), respectively.

Prevalence of SARS-CoV-2 infection among urban cleaning and solid waste management workers during transmission of the Omicron variant in Brazil

Martins-Filho Paulo Ricardo,dos Santos Joyce Thayane da Conceição,Rezende Márcia Santos,de Carvalho Fernanda Oliveira,dos Reis Érica Santos,Barboza Waneska de Souza,Cavalcante Taise Ferreira,dos Sant 한국역학회 2023 Epidemiology and Health Vol.45 No.-

This study estimated the prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in urban cleaning and solid waste management workers during the transmission of the Omicron variant in one of the poorest regions of Brazil (the state of Sergipe). Nasopharyngeal swabs were collected from 494 workers, and the presence of SARS-CoV-2 RNA was tested by quantitative reverse-transcriptase polymerase chain reaction. Data on sociodemographic characteristics, comorbidities, vaccination status, mask use, and use of public transport to commute to the workplace were collected. The prevalence with a 95% confidence interval (CI) was calculated from the proportion of SARS-CoV-2 positive cases among the total number of individuals tested. The prevalence ratio (PR) with a 95% CI was the measure of association used to evaluate the relationship between SARS-CoV-2 infection and the exposure variables. The prevalence of SARS-CoV-2 infection was 22.5% (95% CI, 19.0%–26.4%). Individuals under the age of 40 had a higher prevalence of infection (PR=1.53; 95% CI, 1.03–2.30) as well as those who did not believe in the protective effect of vaccines (PR=1.78; 95% CI, 1.05–2.89). Our results indicate the need for better guidance on preventive measures against COVID-19 among urban cleaning and solid waste management workers.

라틴아메리카의 경제성장과 사회적 소외

윤택동,Reis, Carlos Nelson dos 이베로아메리카연구소 부산외국어대학교 2005 이베로아메리카 Vol.7 No.1

O presente texto contempla uma pontuac¸a~o e ana´lise do crescimento econo^mico e da exclusa~o social nas principais economias da Ame´rica Latina. Para tanto, desenvolve uma breve pontuac¸a~o histo´rica da Regia~o, para na sequ¨e^ncia destacar o comportamento dos agregados que apontam para o crescimento econo^mico e, posteriormente analisa um conjunto de indicadores sociais que apontam para o recrudecimento da questa~o social na Regia~o. Conclui destacando que a Ame´rica Latina teve um vigoroso crescimento econo^mico no se´culo 20, mas, no entanto, na~o logrou diminuir suas histo´ricas desiqualdades sociais. Assim, a caracteri´stica ba´sica da regia~o e´ a coexiste^ncia de inserida no contexto de consumo capitalista e outra exclui´da, em algumas situac¸o~es, ate´ mesmo das necessidades ba´sicas para a sobrevive^ncia. Este problema social, entao, pode afetar diretamente no crescimento economico futuro da Regia~o.

Changes in Invasive Breast Carcinomas after Neoadjuvant Chemotherapy Can Influence Adjuvant Therapeutic Decisions

Bárbara Jaime dos Santos,Débora Balabram,Virginia Mara Reis Gomes,Carolina Costa Café de Castro,Paulo Henrique Costa Diniz,Marcelo Araújo Buzelin,Cristiana Buzelin Nunes 대한암학회 2024 Cancer Research and Treatment Vol.56 No.1

Purpose Neoadjuvant chemotherapy (NACT) can change invasive breast carcinomas (IBC) and influence the patients’ overall survival time (OS). We aimed to identify IBC changes after NACT and their association with OS.Materials and Methods IBC data in pre- and post-NACT samples of 86 patients were evaluated and associated with OS.Results Post-NACT tumors changed nuclear pleomorphism score (p=0.025); mitotic count (p=0.002); % of tumor-infiltrating inflammatory cells (p=0.016); presence of <i>in situ</i> carcinoma (p=0.001) and lymphovascular invasion (LVI; p=0.002); expression of estrogen (p=0.003), progesterone receptors (PR; p=0.019), and Ki67 (p=0.003). Immunohistochemical (IHC) profile changed in 26 tumors (30.2%, p=0.050). Higher risk of death was significatively associated with initial tumor histological grade III (hazard ratio [HR], 2.94), high nuclear pleomorphism (HR, 2.53), high Ki67 index (HR, 2.47), post-NACT presence of LVI (HR, 1.90), luminal B–like profile (HR, 2.58), pre- (HR, 2.26) and post-NACT intermediate mitotic count (HR, 2.12), pre- (HR, 4.45) and post-NACT triple-negative IHC profile (HR, 4.52). On the other hand, lower risk of death was significative associated with pre- (HR, 0.35) and post-NACT (HR, 0.39) estrogen receptor–positive, and pre- (HR, 0.37) and post-NACT (HR, 0.57) PR-positive. Changes in IHC profile were associated with longer OS (p=0.050). In multivariate analysis, pre-NACT grade III tumors and pre-NACT and post-NACT triple negative IHC profile proved to be independent factors for shorter OS.Conclusion NACT can change tumor characteristics and biomarkers and impact on OS; therefore, they should be reassessed on residual samples to improve therapeutic decisions.

Natural Products as Modulators of Mitochondrial Dysfunctions Associated with Cardiovascular Diseases: Advances and Opportunities

Thaise Boeing,Francislaine Aparecida dos Reis Lívero,Priscila de Souza,Danielle Ayr Tavares de Almeida,Guilherme Donadel,Emerson Luiz Botelho Lourenço,Arquimedes Gasparotto Junior 한국식품영양과학회 2023 Journal of medicinal food Vol.26 No.5

The mitochondria have an important role in modulating cell cycle progression, cell survival, and apoptosis. In the adult heart, the cardiac mitochondria have a unique spatial arrangement and occupy nearly one-third the volume of a cardiomyocyte, being highly efficient for converting the products of glucose or fatty acid metabolism into adenosine triphosphate (ATP). In cardiomyocytes, the decline of mitochondrial function reduces ATP generation and increases the production of reactive oxygen species, which generates impaired heart function. This is because mitochondria play a key role in maintaining cytosolic calcium concentration and modulation of muscle contraction, as ATP is required to dissociate actin from myosin. Beyond that, mitochondria have a significant role in cardiomyocyte apoptosis because it is evident that patients who have cardiovascular diseases (CVDs) have increased mitochondrial DNA damage to the heart and aorta. Many studies have shown that natural products have mitochondria-modulating effects in cardiac diseases, determining them as potential candidates for new medicines. This review outlines the leading plant secondary metabolites and natural compounds derived from microorganisms as modulators of mitochondrial dysfunctions associated with CVDs.

Effectiveness and safety of rotary and reciprocating kinematics for retreatment of curved root canals: a systematic review of in vitro studies

Simões Lucas Pinho,Reis-Prado Alexandre Henrique dos,Bueno Carlos Roberto Emerenciano,Viana Ana Cecília Diniz,Duarte Marco Antônio Húngaro,Cintra Luciano Tavares Angelo,Lemos Cleidiel Aparecido Araújo 대한치과보존학회 2022 Restorative Dentistry & Endodontics Vol.47 No.2

Objectives This systematic review (register-osf.io/wg7ba) compared the efficacy and safety of rotary and reciprocating kinematics in the removal of filling material from curved root canals. Materials and Methods Only in vitro studies evaluating both kinematics during retreatment were included. A systematic search (PubMed/MEDLINE, Scopus, and other databases, until January 2021), data extraction, and risk of bias analysis (Joanna Briggs Institute checklist) were performed. Efficacy in filling removal was the primary outcome. Results The search resulted in 2,795 studies, of which 15 were included. Efficacy was measured in terms of the remaining filling material and the time required for this. Nine studies evaluated filling material removal, of which 7 found no significant differences between rotary and reciprocating kinematics. Regarding the time for filling removal, 5 studies showed no difference between both kinematics, 2 studies showed faster results with rotary systems, and other 2 showed the opposite. No significant differences were found in apical transportation, centering ability, instrument failure, dentin removed and extruded debris. A low risk of bias was observed. Conclusions This review suggests that the choice of rotary or reciprocating kinematics does not influence the efficacy of filling removal from curved root canals. Further studies are needed to compare the kinematics safety in curved root canals. Objectives This systematic review (register-osf.io/wg7ba) compared the efficacy and safety of rotary and reciprocating kinematics in the removal of filling material from curved root canals. Materials and Methods Only in vitro studies evaluating both kinematics during retreatment were included. A systematic search (PubMed/MEDLINE, Scopus, and other databases, until January 2021), data extraction, and risk of bias analysis (Joanna Briggs Institute checklist) were performed. Efficacy in filling removal was the primary outcome. Results The search resulted in 2,795 studies, of which 15 were included. Efficacy was measured in terms of the remaining filling material and the time required for this. Nine studies evaluated filling material removal, of which 7 found no significant differences between rotary and reciprocating kinematics. Regarding the time for filling removal, 5 studies showed no difference between both kinematics, 2 studies showed faster results with rotary systems, and other 2 showed the opposite. No significant differences were found in apical transportation, centering ability, instrument failure, dentin removed and extruded debris. A low risk of bias was observed. Conclusions This review suggests that the choice of rotary or reciprocating kinematics does not influence the efficacy of filling removal from curved root canals. Further studies are needed to compare the kinematics safety in curved root canals.

Epizootic Infection by Trypanosoma vivax in Cattle from the State of Minas Gerais, Brazil

Matheus de Oliveira Reis,Fernanda Rezende Souza,Adriana Silva Albuquerque,Fernanda Monteiro,Luan Francisco dos Santos Oliveira,Djeison Lutier Raymundo,Flademir Wouters,Angélica Terezinha Barth Wouter 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.2

Trypanosomiasis is caused by a pathogenic protozoan of the genus Trypanosoma, being Trypanosoma vivax the most important agent for cattle. The aim of the present study was to demonstrate the expansion of T. vivax infection in different mesoregions of Minas Gerais, Brazil, and describe the clinicopathological findings of trypanosomiasis in cattle. The diagnosis was based on visualization of the parasite in blood smears and DNA detection of T. vivax in the blood of live cows and tissues of necropsied animals by the polymerase chain reaction (PCR). Thirty suspected herds were tested, of which 11 were positive for T. vivax. The most frequent clinical signs were anemia, apathy, drop in milk production, weight loss, reproductive disorders, and nervous signs. Concomitant diseases, such as malignant edema, pneumonia and increased cases of mastitis were associated with T. vivax infection. Three cows were necropsied and the most significant findings were low body condition score, pale mucous and spleen with white pulp hyperplasia. The results demonstrated the expansion of T. vivax infection in Minas Gerais, that PCR-associated blood smears are promising for diagnosis, and that other diseases often occur concomitantly to T. vivax infection in regions with trypanosomiasis in cattle.

Can different agents reduce the damage caused by bleaching gel to pulp tissue? A systematic review of basic research

Batista Letícia Aparecida Silva,dos Reis-Prado Alexandre Henrique,Chaves Hebertt Gonzaga dos Santos,de Arantes Lara Cancella,Morgan Luís Fernando Santos Alves,André Carolina Bosso,Suzuki Thaís Yumi,Be 대한치과보존학회 2023 Restorative Dentistry & Endodontics Vol.48 No.4

Objectives: This study aimed to investigate the effectiveness of different topical/systemic agents in reducing the damage caused by bleaching gel to pulp tissue or cells. Materials and Methods: Electronic searches were performed in July 2023. In vivo and in vitro studies evaluating the effects of different topical or systemic agents on pulp inflammation or cytotoxicity after exposure to bleaching agents were included. The risk of bias was assessed. Results: Out of 1,112 articles, 27 were included. Nine animal studies evaluated remineralizing/anti-inflammatories agents in rat molars subjected to bleaching with 35%– 38% hydrogen peroxide (HP). Five of these studies demonstrated a significant reduction in inflammation caused by HP when combined with bioglass or MI Paste Plus (GC America), or following KF-desensitizing or Otosporin treatment (n = 3). However, orally administered drugs did not reduce pulp inflammation (n = 4). Cytotoxicity (n = 17) was primarily assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on human dental pulp cells and mouse dental papilla Cell-23 cells. Certain substances, including sodium ascorbate, butein, manganese chloride, and peroxidase, were found to reduce cytotoxicity, particularly when applied prior to bleaching. The risk of bias was high in animal studies and low in laboratory studies. Conclusions: Few in vivo studies have evaluated agents to reduce the damage caused by bleaching gel to pulp tissue. Within the limitations of these studies, it was found that topical agents were effective in reducing pulp inflammation in animals and cytotoxicity. Further analyses with human pulp are required to substantiate these findings.

Effect of the solid state fermentation of cocoa shell on the secondary metabolites, antioxidant activity, and fatty acids

Ozana Almeida Lessa,Nadabe dos Santos Reis,Selma Gomes Ferreira Leite,Melissa Limoeiro Estrada Gutarra,Alexilda Oliveira Souza,Simone Andrade Gualberto,Julieta Rangel de Oliveira,Elizama Aguiar-Olivei 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.1

During cocoa (Theobroma cacao L.) processing, the accumulated cocoa shell can be used for bioconversion to obtain valuable compounds. Here, we evaluate the effect of solid-state fermentation of cacao flour with Penicillium roqueforti on secondary metabolite composition, phenol, carotenoid, anthocyanin, flavonol, and fatty acids contents, and antioxidant activity. We found that the total concentrations of anthocyanins and flavonols did not change significantly after fermentation and the phenolic compound and total carotenoid concentrations were higher. The fermentation process produced an increase in saponin concentration and antioxidant activity, as well as significant changes in the levels of oleic, linoleic, gamma-linolenic, and saturated fatty acids. Based on our findings, we propose that the reuse of food residues through solid state fermentation is viable and useful.