Long non-coding RNA RP11-6O2.4 indicates poor prognosis and suppresses cell cycle progression through the p38-MAPK signaling pathway in gastric cancer

Yang Feng,Zhiming Fu,Yajun Luo,Wang Tan,Zilin Liu,Pengcheng Ye,Fei Lu,Wanping Xiang,Linghan Tang,Lin Yao,Mengyun Song,Qingmei Huang,Yilun Liu,Jiangwei Xiao 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.3

Backgrounds: The role of long non-coding RNAs (lncRNA) in gastric cancer (GC) has been highlighted in studies conducted over the past decade. However, the potential clinical value and the mechanisms of action of RP11-6O2.4 in GC have not been thoroughly elucidated to date. The specific aim of the present study was to assess RP11-6O2.4 and to explore its role in human GC. Methods: Quantitative real-time polymerase chain reaction (qPCR) was performed to analyze the expression levels of RP11-6O2.4 in GC tissues, paired adjacent noncancerous tissues (ANTs) and GC cell lines. In addition, the correlation between RP11-6O2.4 expression and the clinical characteristics and prognosis of patients with GC was statistically analyzed. The effects of RP11- 6O2.4 on the GC cell cycle transformation through the p38-MAPK signaling pathway were explored by flow cytometry, qPCR and Western blot analysis after treatment with SB203580, a p38MAPK specific inhibitor, in vitro. Results: The expression levels of RP11-6O2.4 in GC tissues were significantly lower than the paired ANTs (P<0.05). In addition, RP11-6O2.4 expression was significantly lower in cases with older age, longer maximum tumor diameter, higher ASA grade and deeper invasive depth (P<0.05). RP11-6O2.4 expression was significantly higher in cases with well/middle differentiation than poor/no differentiation; higher in cases without lymph node metastasis than in lymph node metastasis; and higher in cases in stage Ⅰ/Ⅱ than in stage Ⅲ/Ⅳ. An in vitro assay showed that RP11-6O2.4 induced G0/ G1 phase cell cycle arrest, likely by regulating the p38- MAPK signaling pathway. Conclusion: The above mentioned data suggested that RP11-6O2.4 was a tumor-suppressor gene in GC. RP11- 6O2.4 might play an important role in the cell cycle transformation by regulating the p38-MAPK signaling pathway, thereby representing a specific biomarker and a potential molecular target for the treatment of GC.

Joint Deployment and Trajectory Optimization in UAV-Assisted Vehicular Edge Computing Networks

Zhiwei Wu,Zilin Yang,Chao Yang,Jixu Lin,Yi Liu,Xin Chen 한국통신학회 2022 Journal of communications and networks Vol.24 No.1

As the general mobile edge computing (MEC)scheme cannot adequately handle the emergency communicationrequirements in vehicular networks, unmanned aerial vehicle(UAV)-assisted vehicular edge computing networks (VECNs) areenvisioned as the reliable and cost-efficient paradigm for themobility and flexibility of UAVs. UAVs can perform as thetemporary base stations to provide edge services for road vehicleswith heavy traffic. However, it takes a long time and huge energyconsumption for the UAV to fly from the stay charging stationto the mission areas disorderly. In this paper, we design a predispatchUAV-assisted VECNs system to cope with the demandof vehicles in multiple traffic jams. We propose an optimalUAV flight trajectory algorithm based on the traffic situationawareness. The cloud computing center (CCC) server predictsthe real-time traffic conditions, and assigns UAVs to differentmission areas periodically. Then, a flight trajectory optimizationproblem is formulated to minimize the cost of UAVs, while boththe UAV flying and turning energy costs are mainly considered. Inaddition, we propose a deep reinforcement learning(DRL)-basedenergy efficiency autonomous deployment strategy, to obtain theoptimal hovering position of UAV at each assigned mission area. Simulation results demonstrate that our proposed method canobtain an optimal flight path and deployment of UAV with lowerenergy consumption.

Identification and differential expression of microRNAs in Madin–Darby canine kidney cells with high and low tumorigenicities

Wang Jiamin,Liu Lixia,Yang Di,Zhang Li,Abudureyimu Ayimuguli,Qiao Zilin,Ma Zhongren 한국유전학회 2022 Genes & Genomics Vol.44 No.2

Background: Madin-Darby canine kidney (MDCK) cells are widely used for vaccine production, however, the safety of MDCK cells needs to be considered seriously because of high tumorigenicity. Micro RNAs (miRNAs) that are involved in the tumorigenicity of MDCK cells have been never been reported. Objective: To reveal the role of miRNA in the tumorigenic phenotype of MDCK cell line. Methods: The miRNA expression profiles of two monoclonal MDCK cells (M09CL and M35CL) with low tumorigenicity and one MDCK cell line (M73P) with high tumorigenicity were characterized and investigated by using small RNA-seq technology. Results: A total of 5 known miRNAs and 5 novel miRNAs were highly expressed in M73P. In addition, 4 known miRNAs and 4 novel miRNAs were highly expressed in M09CL and M35CL. The target genes of the differentially expressed miRNAs were significantly enriched in several biological processes, and the majority of these genes were involved in pathways in cancer and the MAPK signaling pathway. Through interaction analysis, 4 up-regulated miRNAs (cfa-miR-452, cfa-miR-8826, cfa-miR-224, and cfa-miR-2387) and their crucial target genes related to the tumor regulation network were identified. Results indicated these 4 miRNAs might play crucial roles in the tumorigenesis of MDCK cells. Conclusion: Our findings, which were based on the functional prediction of miRNAs and target genes, suggested that miRNAs might influence the tumorigenicity of MDCK cells by regulating target genes. Moreover, the results provided important data for understanding the miRNA-mediated regulatory networks that control the tumorigenicities of MDCK cells.

Microstructure and mechanical properties of yttrium aluminum garnet porous ceramics prepared by different sintering process parameters

Yue Liu,Xueqing Yang,Kangliang Peng,Qiong Wang,Jianzhen Huang,Zilin Zhang,Jiang Lu,Hao Xu,Jieguang Song,Lin Chen 한양대학교 세라믹연구소 2019 Journal of Ceramic Processing Research Vol.20 No.4

Yttrium aluminum garnet (YAG), which possesses excellent properties, is investigated and applied. The sintering technology of YAG porous ceramics is optimized. Results show that the porosity initially increases and then decreases with an increase of increasing temperature rate, the porosity are decreased with an increase of removing carbon temperature, the porosity are decreased with an increase of sintering temperature, the porosity are decreased with an increase of holding time. Meanwhile, the compressive strength constantly exhibits an opposite tendency. The optimization of the sintering technology of YAG porous ceramics is based on porosity and compressive strength. A good sintering technology are an increasing temperature rate of 8 oC/min, a removing carbon temperature of 800 oC, removing carbon time of 1 h, a sintering temperature of 1450 oC and holding time of 2 h. The porosity of the prepared YAG porous ceramics is 57.4%, and the compressive strength is 8.89 MPa.

Normalized Creatinine-to-Cystatin C Ratio and Risk of Diabetes in Middle-Aged and Older Adults: The China Health and Retirement Longitudinal Study

Qiu Shanhu,Xue Cai,Bo Xie,Yang Yuan,Sun Zilin,Tongzhi Wu 대한당뇨병학회 2022 Diabetes and Metabolism Journal Vol.46 No.3

Background: Creatinine-to-cystatin C ratio is recently suggested to be a surrogate marker for sarcopenia. However, little is known about its association with diabetes. This study aimed to fill in this gap based on a large-scale prospective cohort.Methods: A population-based representative sample of 5,055 participants aged ≥45 years from the China Health and Retirement Longitudinal Study was enrolled between 2011 and 2012 and followed at least once during the subsequent surveys at 2013, 2015, or 2018. Creatinine-to-cystatin C ratio was calculated and normalized by body weight. Incident diabetes was ascertained by plasma glucose, glycosylated hemoglobin, self-reported history, or use of anti-diabetic drugs. Logistic regression analysis and mediation analysis were employed.Results: During follow-up, 634 participants developed diabetes. The risk of diabetes was gradually and significantly decreased with increased normalized creatinine–cystatin C ratio. The multivariable-adjusted odds ratio for diabetes was 0.91 (95% confidence interval, 0.83 to 0.99) per 1 standard deviation higher of normalized creatinine-to-cystatin C ratio, and this relationship remained significant after controlling for muscle strength. The risk reduction in diabetes was significantly larger in participants with normal-weight and high normalized creatinine-to-cystatin C ratio compared with those with overweight/obesity and high normalized creatinine-to-cystatin C ratio (Pinteraction=0.01). Insulin resistance and inflammation appeared to be key mediators accounting for the observed relationship between normalized creatinine-to-cystatin C ratio and risk of diabetes, with their mediating effect being 93.1% and 22.0%, respectively.Conclusion: High normalized creatinine-to-cystatin C ratio is associated with reduced risk of diabetes in middle-aged and older adults.