MicroRNA Expression Profile Analysis Reveals Diagnostic Biomarker for Human Prostate Cancer

Liu, Dong-Fu,Wu, Ji-Tao,Wang, Jian-Ming,Liu, Qing-Zuo,Gao, Zhen-Li,Liu, Yun-Xiang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Prostate cancer is a highly prevalent disease in older men of the western world. MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression via posttranscriptional inhibition of protein synthesis. To identify the diagnostic potential of miRNAs in prostate cancer, we downloaded the miRNA expression profile of prostate cancer from the GEO database and analysed the differentially expressed miRNAs (DE-miRNAs) in prostate cancerous tissue compared to non-cancerous tissue. Then, the targets of these DE-miRNAs were extracted from the database and mapped to the STRING and KEGG databases for network construction and pathway enrichment analysis. We identified a total of 16 miRNAs that showed a significant differential expression in cancer samples. A total of 9 target genes corresponding to 3 DE-miRNAs were obtained. After network and pathway enrichment analysis, we finally demonstrated that miR-20 appears to play an important role in the regulation of prostate cancer onset. MiR-20 as single biomarker or in combination could be useful in the diagnosis of prostate cancer. We anticipate our study could provide the groundwork for further experiments.

The New Strategy of Object-Based Directional Query

Run-tao liu,Yan-ming Wang,Zhen-guo Zhao,Guang -Yue Tian 보안공학연구지원센터 2014 International Journal of Database Theory and Appli Vol.7 No.6

To increase directional query efficiency, based on the study of existing algorithms of directional query, new pruning rules for directional query were given, combined with the new index structure MB-tree. The rules exclude the MBRs outside the query area and output all leaf nodes in the MBRs inside the query area. Based on the orders defined in MB-tree, a new algorithm of directional query is given combining with MB-tree by using recursive method, and the new algorithm can reduce I/O cost effectively. Experiment showed that the new directional query algorithm reduces the number of visited nodes, decreases I/O cost, and improves the efficiency of directional query.

Size Effect on Surface Roughness in Micro Turning

Tao Zhang,Zhan-qiang Liu,Zhen-yu Shi,Chong-hai Xu 한국정밀공학회 2013 International Journal of Precision Engineering and Vol. No.

The conventional model predicts that the surface roughness decreases with feed and fits well with the measured results, even in the micro turning process. But it is observed that surface roughness increases with feed decreases when the feed is in the range of micro scale. Based on the analysis of peak-to-valley formation process, a quadratic prediction model, which divides the height of peak-tovalley into two parts: one part is piled in front of the rake face while the other is piled on the flank face and is more accurate, is established considering the effect of tool geometry, cutting parameters and pile-up of work piece. The prediction model is calibrated and verified via two groups of micro turning experiments. Results show that size effect of specific cutting energy increases the surface roughness at small feeds. The difference between the theoretical and measured results at small feeds is mainly induced by the pileup of work piece material around the rear face. The best surface roughness can be obtained when the feed per revolution equals 0.1time of the cutting edge radius.

Characterization of a novel variant HMW‐glutenin gene from Elymus canadensis

Qian‐Tao Jiang,Yu‐Ming Wei,Tao Liu,Ji‐Rui Wang,Zhi‐En Pu,Xiu‐Jin Lan,You‐Liang Zheng,Zhen‐Xiang Lu 한국유전학회 2010 Genes & Genomics Vol.32 No.4

High molecular weight (HMW) glutenin subunits (GS) play a key role in the determination of end‐use quality of wheat and other cereal crops. In this study, we report the isolation and characterization of both promoter region and ORF of novel HMW‐GS allele 1St1.3 from a perennial Triticeae species,Elymus canadensis. The amino acid (AA) sequences of E. canadensis 1St1.3 were deduced as 434 aa. Its protein primary structure comprises a signal peptide with a conserved N‐terminal domain, a central repetitive domain and a C‐terminal domain. E. canadensis 1St 1.3 possesses several distinct characteristics which are different from those of wheat HMW‐GSs. The N‐terminal domains of E. canadensis 1St 1.3 resemble that of y‐type subunits, while their C‐terminal domains are more similar to x‐type subunits. The deletion of 85 bp fragment has been observed in promoter region of 1St 1.3, however which has not interrupted the expression of this gene. Our results indicate that 1St 1.3 is novel HMW‐GS variants which will be valuable for enhancing our understanding of structural differentiation and the evolutionary relationship among HMW‐GSs in Triticeae species.

Molecular Characterization of cry Genes from a Bacillus thuringiensis serovar mogi with Mosquitocidal Activity

Qin Liu,Jae Young Choi,Xue Ying Tao,Joo Hyun Lee,Song Eun Kim,Zhenli Fu,Woo Jin Kim,Yeon Ho Je 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10

Bacillus thuringiensis serovar mogi of a novel serogroup (H3a3b3d), which showed mosquitocidal activity against Anopheles sinensis and Culex pipiens pallens, was isolated from fallen leaves in Mungyeong city, Republic of Korea. In contrast to the complicated plasmid profiles of B. thuringiensis H3 serotype strains, the B. thuringiensis serovar mogi contained only megaplasmid (> 30 MDa) on which the toxin genes were occasionally located. Sequence analysis using 454-pyrosequencing revealed that the megaplasmid harbored at least seven putative cry genes, showing about 84%, 75%, 73%, 58%, 84%, 39% and 75% similarities in amino acid sequences with Cry27Aa, Cry19Ba, Cry20-like, Cry56Aa, Cry39ORF2, Cry8Ba and Cry40ORF2, respectively. These cry genes were cloned to the Escherichia coli-B. thuringiensis shuttle vector, pHT1K, and then introduced into an acrystalliferous B. thuringiensis Cry-B strain for further molecular characterization. To investigate the role of these genes in crystal production, the expression profiles of these toxin genes were analyzed by quantitative real-time PCR (qrtPCR) from the wild type strain as well as transformant strains. The results clearly indicate that the cry39orf2 was the dominant ingredient in the crystal. This novel 3a3b3d type strain, B. thuringiensis serovar mogi, could be used as a good resource for studying unknown mosquitocidal cry genes.

Molecular Characterization of the Mosquitocidal cry Genes from a Bacillus thuringiensis Serovar mogi

Qin Liu,Jae Young Choi,Xue Ying Tao,Joo Hyun Lee,Song Eun Kim,Zhenli Fu,Jae Su Kim,Yeon Ho Je 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05

Plasmids from Bacillus thuringiensis have been implicated in pathogenicity as they carry the genes responsible for different types of diseases in mammals and insects. B. thuringiensis serovar mogi of a novel serogroup (H3a3b3d), which showed mosquitocidal activity against Anopheles sinensis and Culex pipiens pallens, was isolated from fallen leaves in Mungyeong city, Republic of Korea. In contrast to the complicated plasmid profiles of B. thuringiensis H3 serotype strains, the B. thuringiensis serovar mogi contained only megaplasmid (> 30 MDa) on which the toxin genes were occasionally located. Sequence analysis using 454-pyrosequencing revealed that the megaplasmid harbored at least seven putative cry genes, showing about 84%, 75%, 73%, 58%, 84%, 39% and 75% similarities in amino acid sequences with Cry27Aa, Cry19Ba, Cry20-like, Cry56Aa, Cry39ORF2, Cry8Ba and Cry40ORF2, respectively. These cry genes were cloned to the Escherichia coli-B. thuringiensis shuttle vector, pHT1K, and then introduced into an acrystalliferous B. thuringiensis Cry-B strain for further molecular characterization. This novel 3a3b3d type strain, B. thuringiensis serovar mogi, could be used as a good resource for studying unknown mosquitocidal cry genes.

Novel High-throughput Baculovirus Expression Vector based on Bombyx mori Nucleopolyhedrovirus

Xue Ying Tao,Jae Young Choi,Woo Jin Kim,Joo Hyun Lee,Qin Liu,Song Eun Kim,Saes Byeol An,Seok Hee Lee,Zhen Li Fu,Yeon Ho Je 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10

The baculovirus expression system is one of the most popular methods used for the production of recombinant proteins but has several complex steps which have proved inherently difficult to meet a multi-parellel process. We have developed a novel recombinant bacmid, bEasyBm that enabling easy and fast generation of pure recombinant virus without any purification step. In the bEasyBm, attR recombination sites were introduced to facilitate the generation of recombinant viral genome by in vitro transposition. Moreover, extracellular RNase gene from bacillus amyloliquefaciens, barnase, was expressed under the control of Cotesia plutellae bracovirus early promoter. Therefore, only when the barnase gene was replaced to gene of interest, the bEasyBm could replicate in host insect cells. When the bEasyBm was transposed with pDualBac-EGFP and pDualBac-LUC respectively, there were no non-recombinant backgrounds were detected from unpurified BmEasy-EGFP or BmEasy-LUC stocks. In addition, the resulting recombinant virus, BmEasy-EGFP, showed comparable level of EGFP expression efficiency with the plaque-purified recombinant virus, BmEGFP, which was constructed using bBmGOZA system. Based on these results, high-throughput condition for generation of multiple recombinant viruses in a time was established.

Preparation of AgCl/Ag₃PO₄/Diatomite Composite by Microemulsion Method for Rapid Photo-Degradation of Rhodamine B with Stability under Visible Light

Zhu, Hai-Tao,Ren, Qi-Fang,Jin, Zhen,Ding, Yi,Liu, Xin-Yu,Ni, Xi-Hui,Han, Meng-Li,Ma, Shi-Yu,Ye, Qing,Oh, Won-Chun Materials Research Society of Korea 2020 한국재료학회지 Vol.30 No.8

In this paper, AgCl/Ag₃PO₄/diatomite photocatalyst is successfully synthesized by microemulsion method and anion in situ substitution method. X-ray diffraction (XRD), photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), and ultraviolet-visible spectroscopy (UV-Vis) are used to study the structural and physicochemical characteristics of the AgCl/Ag₃PO₄/diatomite composite. Using rhodamine B (RhB) as a simulated pollutant, the photocatalytic activity and stability of the AgCl/Ag₃PO₄/diatomite composite under visible light are evaluated. In the AgCl/Ag₃PO₄/diatomite visible light system, RhB is nearly 100 % degraded within 15 minutes. And, after five cycles of operation, the photocatalytic activity of AgCl/Ag₃PO₄/diatomite remains at 95 % of the original level, much higher than that of pure Ag₃PO₄ (40 %). In addition, the mechanism of enhanced catalytic performance is discussed. The high photocatalytic performance of AgCl/Ag₃PO₄/diatomite composites can be attributed to the synergistic effect of Ag₃PO₄, diatomite and AgCl nanoparticles. Free radical trapping experiments are used to show that holes and oxygen are the main active species. This material can quickly react with dye molecules adsorbed on the surface of diatomite to degrade RhB dye to CO₂ and H₂O. Even more remarkably, AgCl/Ag₃PO₄/diatomite can maintain above 95 % photo-degradation activity after five cycles.

Autographa californica Multiple Nucleopolyhedrovirus ac11 is Required for Virus Infection

Xue Ying Tao,Jae Young Choi,Qin Liu,Joo Hyun Lee,Song Eun Kim,Zhenli Fu,Byung Rae Jin,Yeon Ho Je 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05

ORF11 (ac11) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a highly conserved baculovirus gene whose homologs are found in all lepidoteran Group I NPV, but its function is unknown so far. To determine the role of ac11 in baculovirus life cycle, ac11 knock-out mutant, Ac11KO, was constructed using the plasmid capture system (PCS). Real-Time PCR analysis showed that ac11 transcript was first detected at 6 h post-infection (p.i.) and accumulated to maximum at 48 h p.i., indicating that ac11 is belong to late gene. When the genomic DNA of Ac11KO was transfected into Sf9 cells, viral replication was restricted to a cell transfected originally. While viral transmission of the Ac11KO was not observed in Sf9 cells, production of budded virus (BV) in Sf9 cells transfected with Ac11KO was observed by transmission electron microscopy (TEM). These results suggest that the ac11 is essential for AcMNPV to produce infective BV.

Ab initio MRCI+Q Investigations of Spectroscopic Properties of Several Low-lying Electronic States of S2+ Cation

Rui Li,Zhen Zhai,Xiaomei Zhang,Tao Liu,Mingxing Jin,Haifeng Xu,Bing Yan 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.5

The complete active space self-consist field method followed by the internally contracted multireference configuration interaction method has been used to compute the potential energy curves of X2Πg, a4Πu, A2Πu, b4Σ− g, and B2Σ− g states of S2 + cation with large correlation-consistent basis sets. Utilizing the potential energy curves computed with different basis sets, the spectroscopic parameters of these states were evaluated. Finally, the transition dipole moment and the Franck-Condon factors of the transition from A2Πu to X2Πg were evaluated. The radiative lifetime of A2Πu is calculated to be 887 ns, which is in good agreement with experimental value of 805 ± 10 ns.