Immobilization of γ-glutamyltranspeptidase on Silylated Mesoporous TiO2 Whiskers

H. Q. Wang,Zhong Yao,Y. Sun,Z. Zhou,Q. Xiong,Z. X. Zhong 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.2

The surface of mesoporous TiO2 whiskers (MTw)was chemically modified with 3-aminopropyltriethoxysilane(APTES). The point of zero charge (pzc) of MTw and themodified material (MTwA) was 5.3 and 6.8, respectively. MTw and MTwA were then used as carrier to immobilizeγ-glutamyltranspeptidase (GGT) from Bacillus subtilis (B. subtilis). Our results indicated that the loading capacity ofMTwA for GTT was significantly superior to that of MTw. Although the optimum temperature and thermal stability ofMTwA-GGT were slightly lower than those of MTw-GGT,its pH stability was greatly improved compared with eitherfree enzyme or MTw-GGT. The affinity constant (Km) ofMTwA-GGT to γ-glutamyl-p-nitroanilide (GpNA) was0.889 mM, higher than that of free enzyme but lower thanthat of MTw-GGT. In addition, MTwA-GGT displayed agood operational stability.

An accurate and efficient shell element with improved reduced integration rules

Zhong, Z.H.,Tan, M.J.,Li, G.Y. Techno-Press 1999 Structural Engineering and Mechanics, An Int'l Jou Vol.8 No.6

An accurate and efficient shell element is presented. The stiffness of the shell element is decomposed into two parts with one part corresponding to stretching and bending deformation and the other part corresponding to shear deformation of the shell. Both parts of the stiffness are calculated with reduced integration rules, thereby improving computational efficiency. Shear strains are averaged on the reference surface such that neither locking phenomena nor any zero energy mode can occur. The satisfactory behaviour of the element is demonstrated in several numerical examples.

Tensile and Creep Deformation of a Newly Developed Ni-Fe-Based Superalloy for 700 °C Advanced Ultra-Supercritical Boiler Applications

Y. Yuan,Z. H. Zhong,Z.S. Yu,H. F. Yin,Y. Y. Dang,X. B. Zhao,Z. Yang,J. T. Lu,J. B. Yan,Y. Gu 대한금속·재료학회 2015 METALS AND MATERIALS International Vol.21 No.4

A new Ni-Fe-based superalloy, HT-X, has been developed for applications in 700 °C advanced ultra-supercritical (A-USC) boilers. The HT-X alloy is subjected to various heat treatments. Tensile tests are conducted at room temperature (RT), 700 °C and 750 °C. Creep tests are carried out under conditions of 700 °C/300 MPa and 750 °C/150 MPa. After aging treatment, the yield strength of the HT-X alloy at RT and 750 °C is 787 MPa and 624 MPa, respectively. When additional thermal exposure at 750 °C for 5400 h is applied, the yield strength is decreased to 656 MPa at RT and 480 MPa at 700 °C. For an aged specimen, the a/2<110> dislocation shearing process occurs when tensile testing is conducted at RT and 750 °C. As the γ' precipitate size increases in the specimen that is thermally exposed at 750 °C for 5400 h, Orowan bowing is the dominant dislocation process, and stacking faults develop in the γ' precipitates at both RT and 700 °C. Dislocation slip combined with climb is the dominant mechanism under the creep testing conditions. The factors that affect the mechanical properties and deformation mechanisms are discussed.

100KW DC Arc Plasma of CVD System for Low Cost Large Area Diamond Film Deposition

Lu, F.X.,Zhong, G.F.,Fu, Y.L.,Wang, J.J.,Tang, W.Z.,Li, G.H.,Lo, T.L.,Zhang, Y.G.,Zang, J.M.,Pan, C.H.,Tang, C.X.,Lu, Y.P. The Korean Ceramic Society 1996 The Korean journal of ceramics Vol.2 No.4

In the present paper, a new type of DC arc plasma torch is disclosed. The principles of the new magnetic and fluid dynamic controlled large orifice long discharge tunnel plasma torch is discussed. Two series of DC Plasma Jet diamond film deposition equipment have been developed. The 20kW Jet equipped with a $\Phi$70 mm orifice torch is capable of deposition diamond films at a growth rate as high as 40$\mu\textrm{m}$/h over a substrate area of $\Phi$65 mm. The 100kW high power Jet which is newly developed based on the experience of the low power model is equipped with a $\Phi$120 mm orifice torch, and is capable of depositing diamond films over a substrate area of $\Phi$110 mm at growth rate as high as 40 $\mu\textrm{m}$/h, and can be operated at gas recycling mode, which allows 95% of the gases be recycled. It is demonstrated that the new type DC plasma torch can be easily scaled up to even higher power Jet. It is estimated that even by the 100kW Jet, the cost for tool grade diamond films can be as low as less than $4/carat.

Tokamak plasma disruption precursor onset time study based on semi-supervised anomaly detection

X.K. Ai,W. Zheng,M. Zhang,D.L. Chen,C.S. Shen,B.H. Guo,B.J. Xiao,Y. Zhong,N.C. Wang,Z.J. Yang,Z.P. Chen,Z.Y. Chen,Y.H. Ding,Y. Pan Korean Nuclear Society 2024 Nuclear Engineering and Technology Vol.56 No.4

Plasma disruption in tokamak experiments is a challenging issue that causes damage to the device. Reliable prediction methods are needed, but the lack of full understanding of plasma disruption limits the effectiveness of physics-driven methods. Data-driven methods based on supervised learning are commonly used, and they rely on labelled training data. However, manual labelling of disruption precursors is a time-consuming and challenging task, as some precursors are difficult to accurately identify. The mainstream labelling methods assume that the precursor onset occurs at a fixed time before disruption, which leads to mislabeled samples and suboptimal prediction performance. In this paper, we present disruption prediction methods based on anomaly detection to address these issues, demonstrating good prediction performance on J-TEXT and EAST. By evaluating precursor onset times using different anomaly detection algorithms, it is found that labelling methods can be improved since the onset times of different shots are not necessarily the same. The study optimizes precursor labelling using the onset times inferred by the anomaly detection predictor and test the optimized labels on supervised learning disruption predictors. The results on J-TEXT and EAST show that the models trained on the optimized labels outperform those trained on fixed onset time labels.

Investigation on the Unlubricated Sliding Tribological Properties of Ti–20Zr–6.5Al–4V Alloy at Elevated Temperatures

H. Zhong,L. Q. Yang,Y. Yue,C. P. Zhang,F. X. Jin,M. Gu,M. Z. Ma 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.12

In this study, unlubricated sliding friction and wear properties of a recently-developed TiZr-based alloy (Ti–20Zr–6.5Al–4V, TZ20 hereafter) were tested at elevated temperatures ranging from room temperature to 673 K. After the tribologicaltests, worn surface and cross-section of TZ20 alloy were analyzed to illustrate its wear behavior. The results showed that thewear rate was increased firstly with the ambient temperature, which then decreased when the temperature exceeded criticaltransition temperature (473 K). Also, the dominant wear mechanisms changed from adhesive wear at room temperature toabrasive wear at 473 K, and then to mild abrasive wear at highest ambient temperature of 673 K. The variations of wearbehaviors could be attributed to tribo-oxide layer formed during sliding process. At ambient temperature of 673 K, the tribooxidelayer formed on the surface was thicker and more compact, and showed more obvious protective role on tribologicalproperties of TZ20 alloy.

Assessment Of Robot Training for Social Cognitive Learning

H. Y. A. Wong,Z. W. Zhong 제어로봇시스템학회 2016 제어로봇시스템학회 국제학술대회 논문집 Vol.2016 No.10

The number of children diagnosed with autism spectrum disorders (ASD) is on the rise internationally. Most of these children require some degree of special education provision, whether they are educated in regular or specialized settings. Most if not all children with ASD exhibit some impairments with social communication skills. Yet, these skills are associated with outcomes in cognitive functioning and communication. One area employed is social robots to impart social skills in children with ASD. One reason for using social robots is that children with ASD tend to exhibit social behaviors such as eye contact when interacting with robots. This study seeks to examine the extent to which a small and portable robotic platform may be developed to impart social communication skills among children with ASD in specialized educational settings. This study seeks to adapt the social robot for learning social communication skills among children with ASD. In particular, the degree to which these goals can be taught, the speed of skill acquisition, and the degree of intervention will be measured. This research had contributed in three areas. Firstly, this study expounded the idea of “Model of Communication” that could be utilized for constructing social robots that correspond with people and teach social skills. Secondly we found significant improvements in turn-taking skills and more importantly, joint attention. Thirdly, we identified longer duration in eye contact engagement.

SYSTEMATIC SYNTHESIS METHOD AND PROTOTYPING OF FIXEDAXLE VEHICULAR ELECTRIFIED MECHANICAL TRANSMISSION

H. ZHANG,Z. ZHONG,X. CHEN 한국자동차공학회 2015 International journal of automotive technology Vol.16 No.4

In the process of mechanism creative design, structure synthesis is the primary and the most important issue. This paper proposes a systematic and automatic synthesizing method of fixed-axle gear sets by means of morphological analysis. And the process is implemented to synthesize Electrified Mechanical Transmission (EMT) schemes which are parallel hybrid electric transmission composed of fixed-axle gear sets. First, independent design parameters as well as feasibility constraints and consistency conditions are defined by analyzing the structure and functions of EMT. According to these boundary conditions, structure variables such as the quantity of components and their connection relationship are solved. Then, the scheme quantity of each structure type is synthesized. Secondly, based on the abstraction of practical mechanisms with topology models, the corresponding adjacency matrixes are derived, and they are used to filtering unqualified schemes and retrieve all potential transmission paths of the remaining schemes. These potential transmission paths are further used to find out the layout and operating mode of each scheme. Finally, all schemes satisfying the structural and functional requirements are synthesized. For example, one EMT prototype is built based on one of the final schemes found and an ICE car is modified to adapt to such transmission. Vehicle test results verify the effectiveness and feasibility of the chosen transmission scheme.

Roll-to-roll-compatible, flexible, transparent electrodes based on self-nanoembedded Cu nanowires using intense pulsed light irradiation

Zhong, Z.,Woo, K.,Kim, I.,Hwang, H.,Kwon, S.,Choi, Y. M.,Lee, Y.,Lee, T. M.,Kim, K.,Moon, J. Royal Society of Chemistry 2016 Nanoscale Vol.8 No.16

<P>Copper nanowire (Cu NW)-based flexible transparent conductive electrodes (FTCEs) have been investigated in detail for use in various applications such as flexible touch screens, organic photovoltaics and organic light-emitting diodes. In this study, hexadecylamine (HDA) adsorbed onto the surface of NWs is changed into polyvinylpyrrolidone (PVP) via a ligand exchange process; the high-molecular-weight PVP enables high dispersion stability. Intense pulsed light (IPL) irradiation is used to remove organic species present on the surface of the NWs and to form direct connections between the NWs rapidly without any atmospheric control. NWs are self-nanoembedded into a plastic substrate after IPL irradiation, which results in a smooth surface, strong NW/substrate adhesion, excellent mechanical flexibility and enhanced oxidation stability. Moreover, Cu NW FTCEs with high uniformities are successfully fabricated on a large area (150 mm x 200 mm) via successive IPL irradiation that is synchronized with the motion of the sample stage. This study demonstrates the possibility of roll-to-roll-based, large-scale production of low-cost, high-performance Cu NW-based FTCEs.</P>

Influence of crystallization treatment on structure, magnetic properties and magnetocaloric effect of Gd71Ni29 melt-spun ribbons

X.C. Zhong,H.Y. Yu,Z.W. Liu,R.V. Ramanujan 한국물리학회 2018 Current Applied Physics Vol.18 No.11

The influence of crystallization treatment on the structure, magnetic properties and magnetocaloric effect of Gd71Ni29 melt-spun ribbons has been investigated in detail. Annealing of the melt-spun samples at 610 K for 30 min, a majority phase with a Fe3C-type orthorhombic structure (space group, Pnma) and a minority phase with a CrB-type orthorhombic structure (space group, Cmcm) were obtained in the amorphous matrix. The amorphous melt-spun ribbons undergo a second-order ferromagnetic to paramagnetic phase transition at 122 K. For the annealed samples, two magnetic phase transitions caused by amorphous matrix and Gd3Ni phases occur at 82 and 100 K, respectively. The maximum magnetic entropy change (–ΔSM)max is 9.0 J/(kgˑK) (5T) at 122 K for the melt-spun ribbons. The values of (–ΔSM)max in annealed ribbons are 1.0 and 5.7 J/(kgˑK), corresponding to the two adjacent magnetic transitions.