Food allergen sensitization in young children with typical signs and symptoms of immediate-type food allergies: a comparison between monosensitized and polysensitized children

Kim, Na Yeon,Kim, Ga Ram,Kim, Joon Hwan,Baek, Ji Hyeon,Yoon, Jung Won,Jee, Hye Mi,Baek, Hye Sung,Jung, Yong Ho,Choi, Sun Hee,Kim, Ki Eun,Shin, Youn Ho,Yum, Hye Yung,Han, Man Yong,Kim, Kyu-Earn The Korean Pediatric Society 2015 Clinical and Experimental Pediatrics (CEP) Vol.58 No.9

Purpose: The clinical interpretation of children sensitized to allergens is challenging, particularly in children with food allergies. We aimed to examine clinical differences between children with monosensitization and those with polysensitization to common food allergens and to determine risk factors for polysensitization in young children <10 years of age with immediate-type food allergies. Methods: The study included children <10 years of age with signs and symptoms indicative of immediate-type food allergies. Serum total IgE level was measured, and ImmunoCAP analysis for food allergens was performed. Results: The mean age of the study subjects was $1.6{\pm}1.6$ years (75 boys and 51 girls). Thirty-eight children (30.2%) were monosensitized and 88 children (69.8%) were polysensitized. Multivariate logistic regression analysis showed that the development of polysensitization to common food allergens was positively associated with a parental history of allergic rhinitis (adjusted odds ratio [aOR], 6.28; 95% confidence interval [CI], 1.78-22.13; P=0.004), season of birth (summer/fall) (aOR, 3.10; 95% CI, 1.10-8.79; P=0.033), and exclusive breastfeeding in the first 6 months of age (aOR, 3.51; 95% CI, 1.20-10.25; P=0.022). Conclusion: We found significant clinical differences between children with monosensitization and those with polysensitization to common food allergens and identified risk factors for the development of polysensitization in young children with immediate-type food allergies. Clinicians should consider these clinical risk factors when evaluating, counseling, treating, and monitoring young children with food allergies.

Comparison of In Vitro Cell Transformation Assay Using Murine Fibroblasts and Human Keratinocytes

Ahn, Jun-Ho,Park, Sue-Nie,Yum, Yung-Na,Kim, Ji-Young,Lee, Michael Korean Society of ToxicologyKorea Environmental Mu 2008 Toxicological Research Vol. No.

The in vitro cell transformation assays (CTA) were performed using BALB/3T3 murine fibroblasts and HaCaT human keratinocytes in order to evaluate concordance between both in vitro CTAs and carcinogenicity with compounds differing in their genotoxic and carcinogenic potential. Six test articles were evaluated, two each from three classes of compounds: genotoxic carcinogens (2-amino-5-nitrophenol and 4-nitroquinoline-N-oxide), genotoxic noncarcinogens (8-hydroxyquinoline and benzyl alcohol), and nongenotoxic carcinogens (methyl carbamate and N-nitrosodiphenylamine). Any foci of size $\geq$2 mm regardless of invasiveness and piling was scored as positive in CTA with BALB/3T3. As expected, four carcinogens regardless of their genotoxicity had positive outcomes in two-stage CTA using BALB/3T3 cells. However, of the two genotoxic noncarcinogens, benzyl alcohol was positive CTA finding. We concluded that, of the 6 chemicals tested, the sensitivity for BALB/3T3 system was reasonably high, being 100%. The respective specificity for BALB/3T3 assay was 50%. We also investigated the correlation between results of BALB/3T3 assay and results from HaCaT assay in order to develop a reliable human cell transformation assay. However, evaluation of staining at later time points beyond the confluency stage did not yield further assessable data because most of HaCaT cells were detached after $2{\sim}3$ days of confluency. Thus, after test article treatment, HaCaT cells were split before massive cell death began. In this modified protocol for this HaCaT system, growing attached colonies were counted instead of transformed foci 3 weeks since last subculture. Compared to BALB/3T3 assay, HaCaT assay showed moderate low sensitivity and high specificity. Despite these differences in specificity and sensitivity, both cell systems did exhibit same good concordance between in vitro CTA and rodent carcinogenicity findings (overall 83% concordant results). At present the major weakness of these in vitro CTA is lack of validation for regulatory acceptance and use. Thus, more controlled studies will be needed in order to be better able to assess and quantitatively estimate in vitro CTA data.

Comparison of In Vitro Cell Transformation Assay Using Murine Fibroblasts and Human Keratinocytes

Jun-Ho Ahn,Sue Nie Park,Yung-Na Yum,Ji-Young Kim,Michael Lee 한국독성학회 2008 Toxicological Research Vol.24 No.1

The in vitro cell transformation assays (CTA) were performed using BALB/3T3 murine fibroblasts and HaCaT human keratinocytes in order to evaluate concordance between both in vitro CTAs and carcinogenicity with compounds differing in their genotoxic and carcinogenic potential. Six test articles were evaluated, two each from three classes of compounds: genotoxic carcinogens (2-amino-5-nitrophenol and 4-nitroquinoline-N-oxide), genotoxic noncarcinogens (8-hydroxyquinoline and benzyl alcohol), and nongenotoxic carcinogens (methyl carbamate and N-nitrosodiphenylamine). Any foci of size ≥ 2 mm regardless of invasiveness and piling was scored as positive in CTA with BALB/3T3. As expected, four carcinogens regardless of their genotoxicity had positive outcomes in two-stage CTA using BALB/3T3 cells. However, of the two genotoxic noncarcinogens, benzyl alcohol was positive CTA finding. We concluded that, of the 6 chemicals tested, the sensitivity for BALB/3T3 system was reasonably high, being 100%. The respective specificity for BALB/3T3 assay was 50%. We also investigated the correlation between results of BALB/3T3 assay and results from HaCaT assay in order to develop a reliable human cell transformation assay. However, evaluation of staining at later time points beyond the confluency stage did not yield further assessable data because most of HaCaT cells were detached after 2~3 days of confluency. Thus, after test article treatment, HaCaT cells were split before massive cell death began. In this modified protocol for this HaCaT system, growing attached colonies were counted instead of transformed foci 3 weeks since last subculture. Compared to BALB/3T3 assay, HaCaT assay showed moderate low sensitivity and high specificity. Despite these differences in specificity and sensitivity, both cell systems did exhibit same good concordance between in vitro CTA and rodent carcinogenicity findings (overall 83% concordant results). At present the major weakness of these in vitro CTA is lack of validation for regulatory acceptance and use. Thus, more controlled studies will be needed in order to be better able to assess and quantitatively estimate in vitro CTA data.

Effectiveness of the Educational Program in Atopy Camp

조미래 ( Mi Rae Cho ),( So Young Hong ),( Ho Jang Kwon ),( Hye Yung Yum ),( Hyun Jung Kim ),( Soo Hyung Lee ),( Sung Eun Cho ),( Ha Na Cho ),( Ji Eun Jeon ),( Dong Koog Son ) 한국피부장벽학회 2010 한국피부장벽학회지 Vol.12 No.1

Atopic Dermatitis is a complicate disease that accompanies other allergic sicknesses and requires long-term treatment. Therefore, development of an educational program that focuses on management of the disease is needed. This research attempted to enhance the educational effects on patients with atopic dermatitis. Also, as a systematic method to reduce the time and money spent for a through education about the sickness, a concentrated "Atopic Dermatitis Camp" is held at a restricted area to reveal the effectiveness of Atopic Dermatitis camps and the necessity to develop camp education is discussed. This camp provided education and recreational programs on Atopic Dermatitis to those elementary school students who live in Seoul and wants to participate. Over 4 sessions, 151 students participated in the overnight camp. As for the result of the research, a 15-point-scale survey on the Atopic Dermatitis patients` knowledge on the sickness has shown a 10.64 average points before the camp and an 11.87 average after the camp. A statistically significant 1.23 points have risen. It is expected that education about the sickness through the Atopic Dermatitis camp had a positive effect on the patients `knowledge and also that this camp has been an opportunity to recognize the patients` desires to participate in the Atopic Dermatitis camp.

B16-F10 Murine Melanoma 세포의 암전이 억제에 미치는 Diallyl Disulfide의 효과

강미경(Mi-Kyung Kang),전혜승(Hye-Seung Jun),염영나(Yung-Na Yum),황명실(Myung-Sil Hwang),박미선(Mi-Sun Park),김옥희(Ok-Hee Kim) 한국독성학회 2006 Toxicological Research Vol.22 No.4

Diallyl disulfide (DADS), an oil-soluble organosulfur compound in garlic has been reported to suppress tumor growth and to induce apoptosis in cancer. In the present study, we investigated the effects of DADS on pulmonary metastasis of B16-F10 murine melanoma cells. DADS (i.p. 40 ㎎/㎏) significantly (p < 0.05) reduced the number of pulmonary metastatic nodules (48%) in experimental pulmonary metastasis assay. We also found that DADS inhibited adhesion, invasion and migration of B16-F10 melanoma cells in a dose-dependent manner. To study the antimetastatic potential of DADS, we performed the effects of DADS on matrix metalloproteinase activity. DADS significantly inhibited the expression of matrix metalloproteinase-2 activity in B16-F10 cells by gelatin zymography. These results suggest that DADS prevent metastasis in part through suppression of migration of B16-F10 melanoma cells by inhibiting matrix metalloproteinase-2 responsible for degradation of extracellular matrix.

아토피피부염 통합치료 프로그램에서의 심리적 개입의 실제

조성은 ( Sung Eun Cho ),전지은 ( Ji Eun Jeon ),조하나 ( Ha Na Cho ),손동국 ( Dong Koog Son ),김혜원 ( Hye One Kim ),이수형 ( Soo Hyung Lee ),염혜영 ( Hye Yung Yum ) 대한천식알레르기학회 2010 천식 및 알레르기 Vol.30 No.2

Background: Psychological interventions have potentials for improving disease management and quality of life in children with atopic dermatitis (AD) and their families. However, integration of medical and psychological health care in the treatment of children with AD is very rare in Korea. Objective: The aim of this study was to present descriptive data from a hospital-based interdisciplinary program that provides integrated medical and psychological health-care for children with AD. Method: Clinical records were reviewed for 127 children seen in our program as well as common problems and interventions addressed during family visits with the program psychologist. Age group differences in problems and interventions addressed during psychology sessions were evaluated. Also, the differences by symptom severity and sex in problems addressed during psychology sessions were analyzed. Result: Significant age-group differences were found in the problems and interventions addressed during psychology sessions. Among the age groups, sleep problems were most common in the <5 age group (50.0%). Negative self-image, problems of being bullied and attention problems were most common in the 6~12 age group (25.4%, 23.7% and 16.9%, respectively). Anxious/depressed mood, study burden and parent-child relational problems were most common in the 13~18 age group (65.0%, 60.0% and 45.0% respectively). Conclusion: The results of this study support the need for and feasibility of providing integrated care for children with AD and their families. Age-group differences found in problems and interventions addressed during psychology sessions could be useful especially in planning for group sessions. (Korean J Asthma Allergy Clin Immunol 2010; 30:100-109)