Associations of ABCB1 and XPC Genetic Polymorphisms with Susceptibility to Colorectal Cancer and Therapeutic Prognosis in a Chinese Population

Yue, Ai-Min,Xie, Zhen-Bin,Zhao, Hong-Feng,Guo, Shu-Ping,Shen, Yu-Hou,Wang, Hai-Pu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Associations between ABCB1 and XPC genetic polymorphisms and risk of developing colorectal cancer (CRC) as well as clinical outcomes in CRCs with chemotherapy were investigated. A case-control study was performed on the ABCB1 C3435T, G2677T/A and XPC Lys939Gln polymorphisms in 428 CRC cases and 450 hospitalbased, age and sex frequency-matched controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays. We observed that the ABCB1 3435CT or CC+CT variants were significantly linked with increasing risk of developing CRC (adjusted OR (95% CI): 1.814 (1.237-2.660), P=0.0022; adjusted OR (95% CI): 1.605 (1.117-2.306), P=0.0102, respectively). Moreover, the distribution frequency of XPC AC genotype or AC+CC genotypes also showed a tendency towards increasing the suscepbility for CRC (P=0.0759 and P=0.0903, respectively). Kaplan-Meier curves showed that the ABCB1 C3435T variant was associated with a tendency toward longer progression-free survival (PFS) (n=343, Log-rank test: P=0.063), and the G2677T/A variant genotypes (GT+TT+GA+AA) with a tendency for longer OS in postoperative oxaliplatin-based patients (n=343, Log-rank test: P=0.082). However, no correlation of the XPC Lys939Gln polymorphism was found with PFS and OS in patients with postoperative oxaliplatin-based chemotherapy (n=343). Our study indicated that ABCB1 polymorphisms might be candidate pharmacogenomic factors for the prediction of CRC susceptibility, but not for prognosis with oxaliplatin chemosensitivity in CRC patients.

Longitudinal Intrinsic Brain Activity Changes in Cirrhotic Patients before and One Month after Liver Transplantation

Yue Cheng,Li-Xiang Huang,Li Zhang,Ming Ma,Shuang-Shuang Xie,Qian Ji,Xiao-Dong Zhang,Gao-Yan Zhang,Xue-Ning Zhang,Hong-Yan Ni,Wen Shen 대한영상의학회 2017 Korean Journal of Radiology Vol.18 No.2

Objective: To evaluate the spontaneous brain activity alterations in liver transplantation (LT) recipients using resting-state functional MRI. Materials and Methods: Twenty cirrhotic patients as transplant candidates and 25 healthy controls (HCs) were included in this study. All patients repeated the MRI study one month after LT. Amplitude of low-frequency fluctuation (ALFF) values were compared between cirrhotic patients (both pre- and post-LT) and HCs as well as between the pre- and post-LT groups. The relationship between ALFF changes and venous blood ammonia levels and neuropsychological tests were investigated using Pearson’s correlation analysis. Results: In the cirrhotic patients, decreased ALFF in the vision-related regions (left lingual gyrus and calcarine), sensorimotor-related regions (left postcentral gyrus and middle cingulate cortex), and the default-mode network (bilateral precuneus and left inferior parietal lobule) were restored, and the increased ALFF in the temporal and frontal lobe improved in the early period after LT. The ALFF decreases persisted in the right supplementary motor area, inferior parietal lobule, and calcarine. The ALFF changes in the right precuneus were negatively correlated with changes in number connection test-A scores (r = 0.507, p < 0.05). Conclusion: LT improved spontaneous brain activity and the results for associated cognition tests. However, decreased ALFF in some areas persisted, and new-onset abnormal ALFF were possible, indicating that complete cognitive function recovery may need more time.

Effect of activated persulfate on the properties of contaminated soil and degradation behavior of PAHs

Yamin Hong,Xiupeng Yue,Yaping Zhang,Kai Shen,Wentao Jiao,Lei Zhao,Beibei Li 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.3

Sulfate radical-based advanced oxidation processes have been applied in the remediation of polycyclic aromatic hydrocarbons (PAHs) contaminated soil, during which sulfate can be activated in different ways. In this work, effects of four different ways (FeSO4, NaOH, H2O2, and Heat) activated sodium persulfate (PS) on PAHs removal and soil physicochemical properties (pH, organic matter, functional groups, surface morphology, and partial heavy metal elements) were compared, and the influencing factors and mechanism of soil PAHs removal by thermally activated PS were also studied. The results showed that at the dosage of 3% of persulfate, the removal efficiency of PAHs followed the sequences of Heat (91.4%) > FeSO4 (86.6%) > H2O2 (86.2%) > NaOH (72.9%). However, thermal activation decreased the soil pH and organic matter content more significantly than other treatments. The reaction tended to reach equilibrium at 6 h when the dosage of persulfate was 3% and the activation temperature was 60 °C, and the reaction obeyed pseudo-first-order kinetics. Through quenching experiments, it was found that the free radicals playing a dominant role in the oxidation process were sulfate radicals. Compared with pH, liquid to soil (L/S ratio) and the temperature had more significant impacts on the degradation efficiency of PAHs.

CHARACTERIZATION OF THE DUODENASE-1 GENE AND ITS ASSOCIATIONS WITH RESISTANCE TO Streptococuus agalactiae AND GROWTH IN TILAPIA

Yubang Shen,Gui Hong Fu,Feng Liu,Gen Hua Yue 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5

Ginsengenin derivatives synthesized from 20(R)-panaxotriol: Synthesis, characterization, and antitumor activity targeting HIF-1 pathway

Guo, Hong-Yan,Xing, Yue,Sun, Yu-Qiao,Liu, Can,Xu, Qian,Shang, Fan-Fan,Zhang, Run-Hui,Jin, Xue-Jun,Chen, Fener,Lee, Jung Joon,Kang, Dongzhou,Shen, Qing-Kun,Quan, Zhe-Shan The Korean Society of Ginseng 2022 Journal of Ginseng Research Vol.46 No.6

Background: Ginseng possesses antitumor effects, and ginsenosides are considered to be one of its main active chemical components. Ginsenosides can further be hydrolyzed to generate secondary saponins, and 20(R)-panaxotriol is an important sapogenin of ginsenosides. We aimed to synthesize a new ginsengenin derivative from 20(R)-panaxotriol and investigate its antitumor activity in vivo and in vitro. Methods: Here, 20(R)-panaxotriol was selected as a precursor and was modified into its derivatives. The new products were characterized by ¹H-NMR, ¹³C-NMR and HR-MS and evaluated by molecular docking, MTT, luciferase reporter assay, western blotting, immunofluorescent staining, colony formation assay, EdU labeling and immunofluorescence, apoptosis assay, cells migration assay, transwell assay and in vivo antitumor activity assay. Results: The derivative with the best antitumor activity was identified as 6,12-dihydroxy-4,4,8,10,14-pentamethyl-17-(2,6,6-trimethyltetrahydro-2H-pyran-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-yl(tert-butoxycarbonyl)glycinate (A11). The focus of this research was on the antitumor activity of the derivatives. The efficacy of the derivative A11 (IC₅₀ < 0.3 µM) was more than 100 times higher than that of 20(R)- panaxotriol (IC₅₀ > 30 µM). In addition, A11 inhibited the protein expression and nuclear accumulation of the hypoxia-inducible factor HIF-1α in HeLa cells under hypoxic conditions in a dose-dependent manner. Moreover, A11 dose-dependently inhibited the proliferation, migration, and invasion of HeLa cells, while promoting their apoptosis. Notably, the inhibition by A11 was more significant than that by 20(R)-panaxotriol (p < 0.01) in vivo. Conclusion: To our knowledge, this is the first study to report the production of derivative A11 from 20(R)-panaxotriol and its superior antitumor activity compared to its precursor. Moreover, derivative A11 can be used to further study and develop novel antitumor drugs.

An Improved Multiplicative Updating Algorithm for Nonnegative Independent Component Analysis

Hui Li,Yue-hong Shen,Jian-gong Wang 한국전자통신연구원 2013 ETRI Journal Vol.35 No.2

This paper addresses nonnegative independent component analysis (NICA), with the aim to realize the blind separation of nonnegative well-grounded independent source signals, which arises in many practical applications but is hardly ever explored. Recently, Bertrand and Moonen presented a multiplicative NICA (M-NICA) algorithm using multiplicative update and subspace projection. Based on the principle of the mutual correlation minimization, we propose another novel cost function to evaluate the diagonalization level of the correlation matrix, and apply the multiplicative exponentiated gradient (EG) descent update to it to maintain nonnegativity. An efficient approach referred to as the EG-NICA algorithm is derived and its validity is confirmed by numerous simulations conducted on different types of source signals. Results show that the separation performance of the proposed EG-NICA algorithm is superior to that of the previous M-NICA algorithm, with a better unmixing accuracy. In addition, its convergence speed is adjustable by an appropriate user-defined learning rate.

Nematicidal Activity and Chemical Component of Poria cocos

Li, Guo-Hong,Shen, Yue-Mao,Zhang, Ke-Qin The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.1

Poria cocos, a famous traditional Chinese medicine, was found to have nematicidal activity in experiments searching for nematicidal fungi. The experiment showed it could kill 94.9% of the saprophytic nematode, Panagrellus redivivue, 92.6% of the root-knot nematode, Meloidogyne arenaria, and 93.5% of the pine nematode, Bursaphelenchus xylophilus, on PDA plate within 12 hours. According to the nematicidal activity, three new compounds, 2, 4, 6-triacetylenic octane diacid, 2, 4, 5, 6-tetrahydroxyhexanoic acid and 3, 4-dihydroxy-2-keto-n-butyl 2,4,5,6-tetrahydroxyhexanate, were isolated from submerged cultures of Poria cocos. Of these, 2, 4, 6-triacetylenic octane diacid could kill 83.9% Meloidogyne arenaria and 73.4% Panagrellus redivivus at 500 ppm within 12 hours. Here, it is reported for the first time that Poria cocos has nematicidal activity.

Rational Biosynthetic Engineering for Optimization of Geldanamycin Analogues

Kim, Woncheol,Lee, Dongho,Hong, Seong Su,Na, Zhu,Shin, Jin Chul,Roh, Su Heun,Wu, Cheng-Zhu,Choi, Oksik,Lee, Kyeong,Shen, Yue-Mao,Paik, Sang-Gi,Lee, Jung Joon,Hong, Young-Soo WILEY-VCH Verlag 2009 Chembiochem Vol.10 No.7

<P>Tailor made: We report the rational biosynthesis of C15 hydroxylated non-quinone geldanamycin analogues by site-directed mutagenesis of the geldanamycin polyketide synthase (PKS), together with a combination of post-PKS tailoring genes. Rational biosynthetic engineering allowed the generation of geldanamycin derivatives, such as DHQ3 illustrated in the figure, which had superior pharmacological properties in comparison to the parent compound. <img src='wiley_img/14394227-2009-10-7-CBIC200800763-content.gif' alt='wiley_img/14394227-2009-10-7-CBIC200800763-content'> </P><P>A rational biosynthetic engineering approach was applied to the optimization of the pharmacological properties of the benzoquinone ansamycin, geldanamycin. Geldanamycin and its natural or semisynthetic derivatives have the potential to serve as anticancer chemotherapeutic agents. However, these first-generation Hsp90 inhibitors share an unfavorable structural feature that causes both reduced efficacy and toxicity during clinical evaluation. We report the rationally designed biosynthesis of C15 hydroxylated non-quinone geldanamycin analogues by site-directed mutagenesis of the geldanamycin polyketide synthase (PKS), together with a combination of post-PKS tailoring genes. A 15-hydroxyl-17-demethoxy non-quinone analogue, DHQ3, exhibited stronger inhibition of Hsp90 ATPase activity (4.6-fold) than geldanamycin. Taken together, the results of the present study indicate that rational biosynthetic engineering allows the generation of derivatives of geldanamycin with superior pharmacological properties.</P> <B>Graphic Abstract</B> <P>Tailor made: We report the rational biosynthesis of C15 hydroxylated non-quinone geldanamycin analogues by site-directed mutagenesis of the geldanamycin polyketide synthase (PKS), together with a combination of post-PKS tailoring genes. Rational biosynthetic engineering allowed the generation of geldanamycin derivatives, such as DHQ3 illustrated in the figure, which had superior pharmacological properties in comparison to the parent compound. <img src='wiley_img/14394227-2009-10-7-CBIC200800763-content.gif' alt='wiley_img/14394227-2009-10-7-CBIC200800763-content'> </P>

Convolutive Independent Component Analysis Based on First-order Statistics for Complex-valued Source Extraction

Peng-cheng Xu,Yue-hong Shen 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.1

This paper addresses the problem of independent component extraction of complex-valued signals in convolutive mixtures. Most previous research focused on real-valued convolutive ICA, and corresponding solution methods are generally computationally complex and inefficient for real application. In order to solve the problem, we propose a novel method based on first-order statistics, which includes several single-step and iterative separators to satisfy different demands of engineering applications. We also provided the theoretical performance analysis, which is validated by experimental simulations. It is observed from the simulations that various factors (especially the noncircularity) affect the extraction performance of separators; hence we offer some advice on how to choose separators properly. Besides, the proposed iterative separators generally perform better and converge faster compared to two complex FastICA algorithms.

Mechanical Behavior of Glass Panels Supported by Clamping Joints in Cable Net Facades

Ruo-qiang Feng,Ji-hong Ye,Yue Wu,Shi-zhao Shen 한국강구조학회 2012 International Journal of Steel Structures Vol.12 No.1

In glass panels supported by clamping joints in a cable net facade, the clamping joint restrains movement at the four corners of the glass panel. As a result, the mechanical behavior of the glass panels supported by clamping joints is very different than the behavior of glass panels supported by four hinged corners. Moreover, the deformation in cable net facade is large and nonuniform; thus, the glass panels in cable net facades bear the effects of warping distortion. In this study, a model test and theoretical analysis was conducted to study the mechanical behavior of glass panels supported by clamping joints in a cable net facade. To this end, a cable net facade was designed, and a proper experimental model was constructed. A static mechanical model test was conducted, where the glass stress at different positions in the cable net was measured. A numerical model of the cable net facade was developed, and the results of the experiment and the numerical analysis were compared. Furthermore,the numerical results provided a detailed description of the mechanical behavior of the glass panels in a cable net facade.