Violation of DNA neighbor exclusion principle in RNA recognition

Yousuf, Muhammad,Youn, Il Seung,Yun, Jeonghun,Rasheed, Lubna,Valero, Rosendo,Shi, Genggongwo,Kim, Kwang S. Royal Society of Chemistry 2016 Chemical science Vol.7 No.6

<▼1><P>DNA intercalation has been very useful for engineering DNA-based functional materials.</P></▼1><▼2><P>DNA intercalation has been very useful for engineering DNA-based functional materials. It is generally expected that the intercalation phenomenon in RNA would be similar to that in DNA. Here we note that the neighbor-exclusion principle is violated in RNA by naphthalene-based cationic probes, in contrast to the fact that it is usually valid in DNA. All the intercalation structures are responsible for the fluorescence, where small naphthalene moieties are intercalated in between bases <I>via</I> π–π interactions. The structure is aided by hydrogen bonds between the cationic moieties and the ribose-phosphate backbone, which results in specific selectivity for RNA over DNA. This experimentally observed mechanism is supported by computationally reproducing the fluorescence and CD data. MD simulations confirm the unfolding of RNA due to the intercalation of probes. Elucidation of the mechanism of selective sensing for RNA over DNA would be highly beneficial for dynamical observation of RNA which is essential for studying its biological roles.</P></▼2>

Esterification of acetic and oleic acids within the Amberlyst 15 packed catalytic column

Yousuf Jamal,Jamshed Ali Khan,Afeeq Shahid,O’Neil Boulanger 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.2

A packed column system was used to study the esterification of acetic and oleic acids by the macro-porous acidic resin, Amberlyst 15. All reactions were at a constant temperature (75 oC) and catalyst mass (3 g). The impact of column flow rate conditions and the molar fatty acid to ethanol feedstock ratio on ester production is reported. The maximum ester production was noted at a flow rate of 0.25mL/min. The maximum observed ester yield for acetic acid (95.2±0.5%) and oleic acid (43.8±1.3%) was observed at an acid:ethanol molar ratio of 1 : 3 and 1 : 1, respectively. The difference in yield indicates the importance of the fatty acid chain length to the reaction.

Precise Tuning of Cationic Cyclophanes toward Highly Selective Fluorogenic Recognition of Specific Biophosphate Anions

Yousuf, Muhammad,Ahmed, Nisar,Shirinfar, Bahareh,Miriyala, Vijay Madhav,Youn, Il Seung,Kim, Kwang S. American Chemical Society 2014 ORGANIC LETTERS Vol.16 No.8

<P>Cationic cyclophanes with bridging and spacer groups possess well-organized semirigid cavities and are able to encapsulate and stabilize anionic species through diverse molecular interactions. We highlight the precise tuning of functionalized cyclophanes toward selective recognition of AMP, GTP, and pyrophosphate (PPi) using fluorescence, NMR spectroscopy, and density functional theory (DFT).</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/orlef7/2014/orlef7.2014.16.issue-8/ol500613y/production/images/medium/ol-2014-00613y_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ol500613y'>ACS Electronic Supporting Info</A></P>

Role of asymptomatic hyperuricemia in the progression of chronic kidney disease and cardiovascular disease

( Yousuf Waheed ),( Fan Yang ),( Dong Sun ) 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.6

Previous research has investigated whether hyperuricemia serves as an independent risk factor for cardiovascular and renal diseases. Hyperuricemia is defined as an abnormally high level of uric acid (UA; i.e., serum urate level > 6.8 mg/dL). Hyperuricemia has been considered a complication of chronic kidney disease (CKD). However, it seems to play a pathogenic role in the progression of renal diseases. There has been increasing focus on the link between hyperuricemia and CKD. The results of randomized controlled trials have implied independent associations between hyperuricemia and the progression of cardiovascular and renal morbidities. These associations may be mediated by renin-angiotensin system activation, nitric oxide synthase inhibition, and macrovascular/microvascular disease development. There remains controversy regarding the use of serum UA level as an indirect index of renal vascular disease. This literature review focuses on the role of asymptomatic hyperuricemia in the progression of CKD, as well as the association between hyperuricemia and cardiovascular disease. It also provides a general overview of the physiological metabolism of UA.

Comparison of One and Two Step Esterification Method for Wastewater Sludge Lipid Conversion to Biodiesel

( Yousuf Jamal ),( Jae-hyop Son ),( Sang-hyeop Cha ),( In-sung Jo ),( Nguyen Viet Pham ),( Hung-suck Park ) 한국폐기물자원순환학회(구 한국폐기물학회) 2015 한국폐기물자원순환학회 3RINCs초록집 Vol.2015 No.-

Lipids account for 30-40% of the biological and chemical oxygen demand in wastewater treatment plant influents. High amounts of lipids are also present in microbial communities that treat the wastewater in typical conventional activated sludge plants. Production of biodiesel from high price pure triglyceride based feed stock is economically not feasible although conversion technology is mature in Korea. Keeping this idea of using low cost feedstock for biodiesel production a laboratory scale experimental work was setup while using different solvents for lipid extraction and their conversion to mono-alkyl esters also known as biodiesel. Some of the parameters studied for one and two step conversion were ex situ and in situ extraction of lipids, using the low cost polar and non-polar solvents, alcohol to lipid molar ratio, volume of homogeneous acidic catalyst required for optimized conversion. It was found that solvent type used for lipid extraction from wastewater sludge has a major impact on the saponifiable oil extraction, along with the moisture content of the sludge. Extraction temperature also influences the oil content in the extract. Increased extraction yield of 72% oil was noted with a mixture of polar and non- polar solvents. Highest ester yield of 95% recorded in two hours with one step esterification method.

복잡한 천부구조하에서 반사법 탄성파자료의 반복성에 대한 평가, 호주, 빅토리아, CO2CRC Otway 프로젝트

( Yousuf Al Jabri ),( Milovan Urosevic ) 한국지구물리·물리탐사학회 2010 지구물리와 물리탐사 Vol.13 No.1

Do Saudi Nurses in Primary Health Care Centres have Breast Cancer Knowledge to Promote Breast Cancer Awareness?

Yousuf, Shadia Abdullah,Al Amoudi, Samia Mohammed,Nicolas, Wafa,Banjar, Hasna Erfan,Salem, Safaa Mohammed Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Background: Breast cancer is one of the leading causes of death among cancer patients in Saudi Arabia. It is known that nurses play a key role in promoting breast cancer awareness among women in any society. Nurses in primary health care centres (PHCC) have more direct contact with general population. This study aimed to investigate nurse knowledge of breast cancer and practice of early screening in PHCC in Jeddah city. Methods: A cross-sectional study was conducted using a self-administered questionnaire to assess the general knowledge of breast cancer, risk factors, and practice of 210 PHCC nurses. Data were analysed using SPSS v.16. Results: The mean age of the PHCC Nurses was 36.9 (SD ${\pm}8.6$). Only 11% percent scored <50% of the total score for general epidemiological knowledge on breast cancer, about 35% scored <50% of the total score for breast cancer risk factors. Sixty seven percent scored >75% of the total score on breast cancer signs. Majority of nurses 62.8% practiced BSE, but only 4% practiced regularly every month. Some 28% practiced once per year. About 81% of the nurses had not had a clinical breast examination and only 14% had had a mammography. The results of the study failed to show any correlation between the knowledge scores with age, education, year of experience, family history of BC and marital status of the nurses. Conclusion:The results from the study reflect that there is a need to provide continuing nursing education programmes for PHCC nurses to improve their breast cancer knowledge and practice.

Electromagnetic Performance of Five Phase Non-Overlapping Stator Wound Field Flux Switching Machine

Yousuf Muhammad,Khan Faisal,Ullah Basharat 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.1

The multi-phase wound fi eld fl ux switching (WFFS) machines achieved the advantages of the conventional FSPM machines and the common merits of multi-phase machines, e.g., low torque pulsations, high torque density and high fault tolerant capability. The non-overlapping windings can be used to minimize the consumption of copper and manufacturing costs for the WFFS machines. Hence, in this paper, fi ve phase WFFS machines having the non-overlapping winding topology with four diff erent rotor poles is presented. Ten slots with four diff erent rotor pole designs namely 10S-12P, 10S-11P, 10S-9P, and 10S8P are chosen to analyze the rotor pole study. The main section explains an essential overview of load and no-load analyses for fi ve Phase WFFS machines and designs with diff erent rotor poles are briefl y compared. The proposed machine design studies are analyzed with fi ve phase behavior of machine based on 2D-FEA. A detailed analysis of the proposed designs is also discussed like fl ux linkage, cogging torque, back EMF, THD, average torque versus current density, instantaneous torque, average torque versus stack length, copper losses, iron losses, effi ciency and inductance calculations. The simulated results elaborate that the design with 11 rotor poles is best as it produces better back emf, higher torque and effi ciency of 3.2 V, 1.65 Nm and 82.4% respectively.

The ICAM-1 Gly241Arg Polymorphism is Not Associated With Polycystic Ovary Syndrome - Results from a Case Control study in Kashmir, India

yousuf, Syed Douhath,Ganie, Mohammad Ashraf,Zargar, Mohammad Afzal,Parvez, Tabasum,Rashid, Fouzia Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3

Background: Polycystic ovary syndrome (PCOS) is considered to be a multifactorial disorder resulting from the interaction of several predisposing and protective genetic variants. PCOS is associated with low-grade chronic inflammation. Elevated levels of inflammatory markers including intercellular adhesion molecule-1 (ICAM-1) are demonstrated in women with PCOS. Recent evidence indicates a significant linkage between a locus on chromosome 19p13 and multifactorial diseases that have an inflammatory component. The aim of the study was to assess the possible association of Gly241Arg polymorphism of ICAM-1 gene located on chromosome 19p13 in determining risk of PCOS in Kashmiri women. Materials and Methods: Gly241Arg SNP in DNA from peripheral blood leukocytes of 220 PCOS cases and 220 age matched non-PCOS healthy controls was analysed using allel specific PCR. Results: The genotype and allele frequency distributions of Gly241Arg SNP showed insignificant difference between the PCOS cases and control women, indicating no role of this SNP in PCOS susceptibility. The odds ratio for Arg/Arg genotype was 0.87 (95% CI=0.32-2.3) [P=0.79], for Gly/Arg genotype was 0.98 (95% CI= 0.66-1.47) [P=1] and for Arg/Arg+Gly/Arg genotype was 0.97 (95% CI=0.65-1.45) [P=0.92]. The genotypic frequencies of ICAM-1codon 241 showed statistically insignificant difference between cases and controls (${\chi}^2=0.07$; p=0.96) Nor the studied polymorphism was found to affect clinical and laboratory parameters significantly. Conclusions: Although Gly241Arg polymorphism have not shown significant association with PCOS. Further, specifically designed studies on large cohorts are required to conclusively establish any role of ICAM-1 gene polymorphisms in PCOS in our study.

A Review on Floating Photovoltaic Technology (FPVT)

Hasnain Yousuf,Muhammad Quddamah Khokhar,Muhammad Aleem Zahid,Jaeun Kim,김영국,조은철,조용현,이준신 한국태양광발전학회 2020 Current Photovoltaic Research Vol.8 No.3

A novel energy production system which has fascinated a wide consideration because of its several benefits that are called floating photovoltaic technology (FPVT). The FPVT system that helps to minimize the evaporation of water as well as an increase in energy production. For the research purposes, both electrical and mechanical structure requires studying of these systems for the development of FPVT power plants. From different points of views, numerous researches have been directed on FPVT systems that have evaluated these systems. The present research article give a logical investigation and up to date review that shows the different features and components of FPVT systems as an energy production system is offered. This articles reviewing the FPVT that gets the attention of the scientists who have the investigational stage and involuntary inspection of FPVT systems in addition to influence of implementing these systems on the water surface. Also, a comprehensive comparison has been constructed that shows the cons and pros of various types of solar systems that could be installed in various locations. In this review, it has been found that solar energy on the roof of a dwelling house generally has a power of 5 to 20 kW, while the inhabitants of commercial buildings generally have a power of 100 kW or more. The average power capacity of a floating solar panel is 11% more of the average capacity of a solar panel installed on the ground. Studies show that 40% of the water in open reservoirs is lost through evaporation. By covering only 30% of the water surface, evaporation can be reduced by 49%. The global solar panel market exceeds 100 GW and the capacity of 104 GW will bring the annual growth rate to 6%. In 2018, the world's total photovoltaic capacity reached 512 GW, an increase of 27% compared to the total capacity and about 55% of the renewable resources newly created that come from photovoltaic systems. It has been also predicted by this review that in 2025 the Solar technology including the FPVT system will increase by 7.38% that is 485.4 GW more of today installed power worldwide.