Effect of Different Potassium Level on Growth and Fruit Yield of Camellia Oleifera Chang-Lin Series

Lu You,Zhi Li,Dekui Niu,Dongnan Hu,Wenyuan Zhang,Xiaomin Guo,Deyue Meng 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Role of a Novel Pyridostigmine Bromide-Phospholipid Nanocomplex in Improving Oral Bioavailability

Qun-you Tan,Jing-qing Zhang,Ni-ni Hu,Guo-dong Liu,Hua-feng Yin,Li Zhang,Hong Wang,Lu-yang Lu 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.3

A novel pyridostigmine bromide (PB)-phospholipid nanocomplex (PBPLC) was prepared to increase the bioavailability of PB. A central composite design approach was employed for process optimization. The physicochemical properties of PBPLC were investigated by means of differential scanning calorimetry, ultraviolet spectroscopy, Fourier transformed infrared spectroscopy and the n-octano/water partition coefficient. The intestinal permeability of PBPLC was observed via a single pass intestinal perfusion in rats. After oral administration of PBPLC, the concentrations of PB at predetermined time points were determined by HPLC, and the pharmacokinetic parameters were computed by DAS 2.1.1 software. Multiple linear regression analysis for process optimization revealed that the optimal PBPLC was obtained when the values of X1, X2, and X3 were 8, 40oC, and 4 mg/mL, respectively. The average particle size and zeta potential of PBPLC with the optimized formulation were 204.60 nm and −25.12 mV, respectively. Non-covalent interactions between PB and phospholipids were found in the PBPLC. The n-octanol/water partition coefficient of PBPLC was substantially increased. PBPLC had better intestinal permeability in comparison with free PB. Mean plasma drug concentration-time curves of PBPLC and free PB after oral administration were both in accordance with the two-compartment open model. The values of pharmacokinetic parameters of PBPLC and free PB were the peak time (Tmax) 2 h vs 2 h, the maximum concentration (Cmax) 22.79 μg/mL vs 6.00 μg/mL, and the value of the area under the concentration vs time curve (AUC0-∞) 7128.21 μg·min/mL vs 1772.36 μg·min/mL, respectively. In conclusion, compared with free PB, PBPLC remarkably improves the oral bioavailability of PB, which is likely due to its higher lipophilicity and permeability.

Construction of a genetic linkage map and QTL mapping of agronomic traits in Auricularia auricula-judae

Li-Xin Lu,Fang-Jie Yao,Peng Wang,Ming Fang,You-Min Zhang,Wei-Tong Zhang,Xiang-Hui Kong,Jia Lu 한국미생물학회 2017 The journal of microbiology Vol.55 No.10

Auricularia auricula-judae is a traditional edible fungus that is cultivated widely in China. In this study, a genetic linkage map for A. auricula-judae was constructed using a mapping population consisting of 138 monokaryons derived from a hybrid strain (A119-5). The monokaryotic parent strains A14-5 and A18-119 were derived from two cultivated varieties, A14 (Qihei No. 1) and A18 (Qihei No. 2), respectively. In total, 130 simple sequence repeat markers were mapped. These markers were developed using the whole genome sequence of A. auricula-judae and amplified in A14-5, A18- 119, and the mapping population. The map consisted of 11 linkage groups (LGs) spanning 854 cM, with an average interval length of 6.57 cM. A testcross population was derived from crossing between the monokaryon A184-57 (from the wild strain A184 as a tester strain) and the mapping population. Important agronomic trait-related QTLs, including mycelium growth rate on potato dextrose agar for the mapping population, mycelium growth rate on potato dextrose agar and sawdust for the testcross population, growth period (days from inoculation to fruiting body harvesting), and yield for the testcross population, were identified using the composite interval mapping method. Six mycelium growth raterelated QTLs were identified on LG1 and LG4, two growth period-related QTLs were identified on LG2, and three yieldrelated QTLs were identified on LG2 and LG6. The results showed no linkage relationship between mycelium growth rate and growth period. The present study provides a foundation for locating genes for important agronomic characteristics in A. auricula-judae in the future.

Acetylation Enhances the Promoting Role of AIB1 in Breast Cancer Cell Proliferation

You, Dingyun,Zhao, Hongbo,Wang, Yan,Jiao, Yang,Lu, Minnan,Yan, Shan Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.9

The oncogene nuclear receptor coactivator amplified in breast cancer 1 (AIB1) is a transcriptional coactivator, which is overexpressed in various types of human cancers, including breast cancer. However, the molecular mechanisms regulating AIB1 function remain largely unknown. In this study, we present evidence demonstrating that AIB1 is acetylated by MOF in human breast cancer cells. Moreover, we also found that the acetylation of AIB1 enhances its function in promoting breast cancer cell proliferation. We further showed that the acetylation of AIB1 is required for its recruitment to E2F1 target genes by E2F1. More importantly, we found that the acetylation levels of AIB1 are greatly elevated in human breast cancer cells compared with that in non-cancerous cells. Collectively, our results shed light on the molecular mechanisms that regulate AIB1 function in breast cancer.

A Privacy-preserving and Energy-efficient Offloading Algorithm based on Lyapunov Optimization

Lu Chen,Hongbo Tang,Yu Zhao,Wei You,Kai Wang 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.8

In Mobile Edge Computing (MEC), attackers can speculate and mine sensitive user information by eavesdropping wireless channel status and offloading usage pattern, leading to user privacy leakage. To solve this problem, this paper proposes a Privacy-preserving and Energy-efficient Offloading Algorithm (PEOA) based on Lyapunov optimization. In this method, a continuous Markov process offloading model with a buffer queue strategy is built first. Then the amount of privacy of offloading usage pattern in wireless channel is defined. Finally, by introducing the Lyapunov optimization, the problem of minimum average energy consumption in continuous state transition process with privacy constraints in the infinite time domain is transformed into the minimum value problem of each timeslot, which reduces the complexity of algorithms and helps obtain the optimal solution while maintaining low energy consumption. The experimental results show that, compared with other methods, PEOA can maintain the amount of privacy accumulation in the system near zero, while sustaining low average energy consumption costs. This makes it difficult for attackers to infer sensitive user information through offloading usage patterns, thus effectively protecting user privacy and safety.

선물의 역할(giver-receiver)에서 관계적 특성이 해석 수준(construal level) 선호도 차이에 관한 연구

유인식 ( You In Sik ),이동명 ( Lee Dong Myung ),진로 ( Chen Lu ) 한국소비자학회 2019 소비자학연구 Vol.30 No.4

선물의 선호도는 주는 사람과 받는 사람의 두가지 해석 수준에 따라 달라진다. 첫 번째 해석 수준은 가치와 목적에 치중하는 즉 왜(Why)에 가까운 바람직성(desirability)이며, 두 번째는 사용 수단과 용이성에 치중하는 즉 어떻게(how)와 밀접한 실행가능성(feasibility)으로 구분된다. 이에 본 연구에서는 선물을 주는 사람과 받는 사람에 따라 어떠한 해석 수준의 선물을 선호하는지를 살펴보았다. 또한 관계적 특성을 사회적 관계와 심리적 친밀도로 구분하여, 관계적 특성에 따라 어떠한 해석 수준의 선물을 선호하는지를 추가적으로 심도 있게 연구하였다. 선물을 주는 사람과 받는 사람의 상황 별로 각 150명을 설문하여 유효 응답자 291명을 대상으로 연구한 결과, 다음과 같은 연구결과를 도출하였다. 첫째, 관계적 특성을 고려하지 않은 상황에서는 미국의 선물 문화에 대한 선행연구와 동일하게, 선물을 주는 사람은 바람직성이 높은 선물을 선호하였으며 받는 사람은 실행가능성이 높은 선물을 선호하였다. 둘째, 친밀도가 높은 가족, 친척, 친구의 관계에서는 받는 사람과 주는 사람 모두 실행 가능성이 높은 선물을 선호하였다. 세 번째 친밀도가 낮은 사적인 관계인 경우 주는 사람과 받는 사람 모두 바람직성이 높은 선물을 선호하였으며 이는 한국의 선물 문화에서 공동체의식과 체면 의식이 반영된 의무적인 선물 상황에서 발생하는 것으로 추정되며 반면에 공적인 관계에서는 친밀도가 높은 경우 주는 사람과 받는 사람 모두 실행 가능성이 높은 선물을 선호하였으며 친밀도가 낮은 경우 주는 사람은 바람직성이 높은 선물을 받는 사람은 실행 가능성이 높은 선물을 선호하였다. 이러한 연구 결과는 관계 특성과 친밀도가 선물 해석 수준 선호도 차이가 있음을 규명하였고 학문적이고 실무적인 시사점을 제공하였다. Gift preference depends on the construal level of two people, giver and receiver. 1st construal level depends on the value and the purpose. In other words, desirability close to why. 2nd construal level is about means of use and convenience. So, this study observes which gift is preferred depends on giver and receiver as per the level of interpretation of gift. Also, by distinguishing the characteristic of relationship depends on social relationship and emotional closeness, additional in-depth study on gift preference is done. Study on 150 of receiver and giver respectively, 291 responses were selected, and we have the following results. 1st, without considering relationship, similar to gift culture in the States, giver prefers a gift with high desirability and receiver prefers a gift with high level of feasibility. 2nd, within close family, friends and friends, both receiver and giver prefers a gift with high level of feasibility. 3rd, in a personal relationship with low closeness, both giver and receiver prefer a gift with high desirability. This seems to reflect Korean gifting culture where community spirit and respect for each other exists. In public relations, between giver and receiver who are close prefer a gift with high feasibility while who are not close prefer a gift with desirability. This study reveals the level of relationship and the closeness affects the interpretation of gift preference and provides the academical implication.

A novel pattern recognition protein of the Chinese oak silkmoth, Antheraea pernyi, is involved in the pro-PO activating system

Xia Lu Wan,Zhang Jing Hai,Ying Chen,You Lei Ma,Wen Jun Zou,Guo Yuan Ding,Wei Li,Ming Yi Zhao,Chun Fu Wu 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.7

Association Between the Ku70-1310C/G Promoter Polymorphism and Cancer Risk: a Meta-analysis

Xu, Lu,Ju, Xiao-Bing,Li, Pu,Wang, Jue,Shi, Zhu-Mei,Zheng, Ming-Jie,Xue, Dan-Dan,Xu, Yan-Jie,Yin, Yong-Mei,Wang, Shui,You, Yong-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

Ku70 plays an important role in DNA double-strand break repair. Studies revealing conflicting results on the role of the Ku70-1310C/G promoter polymorphism on cancer risk led us to perform a meta-analysis to investigate this relationship. Ten case-control studies with 2566 cases and 3058 controls were identified. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of associations. The overall results suggested no association between the Ku70-1310C/G promoter polymorphism and total cancer risk. However, on stratified analysis, significantly increased risks were observed among the Asian population (GG vs. CC: OR=1.50, 95%CI=1.10-2.06; GG vs. CC/CG: OR=1.47, 95%CI=1.07-2.01) and population-based case-control studies (GG vs. CC: OR=1.57, 95%CI=1.12-2.22; CG vs. CC: OR=1.35, 95%CI=1.11-1.64; CG/GG vs. CC: OR=1.37, 95%CI=1.14-1.65). Additionally, variant genotypes were associated with a significantly increased breast cancer risk (GG vs. CC: OR=1.80, 95%CI=1.26-2.56; GG vs. CC/CG: OR=1.40, 95%CI=1.01-1.95).

The Blood Neutrophil-to-lymphocyte Ratio Predicts Survival in Patients with Advanced Hepatocellular Carcinoma Receiving Sorafenib

Zheng, You-Bing,Zhao, Wei,Liu, Bing,Lu, Li-Gong,He, Xu,Huang, Jian-Wen,Li, Yong,Hu, Bao-Shan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Background and Aim: Increasing evidence correlates the presence of systemic inflammation with poor survival in patients with hepatocellular carcinoma (HCC). The aim of this study was to investigate the prognostic significance of the blood neutrophil-to-lymphocyte ratio (NLR) in patients with advanced HCC who received sorafenib monotherapy. Methods: A total of sixty-five patients with advanced HCC, not eligible for locoregional therapy, treated with sorafenib were enrolled. Potential prognostic factors such as age, gender, tumoral characteristics, performance status and NLR were analyzed. Results: Median OS and TTP for the entire cohort were 10.0 months (95%CI, 7.6-12.3 months) and 4.5 months (95% CI, 4.0-4.9 months). The mean NLR at baseline was 2.89. The median OS of patients with a high NLR (>4) was 6.5 months (95%CI, 5.2-7.7 months) compared with 12.5 months (95%CI, 9.9-15.0) for patients with a normal NLR (${\leq}4$) (P=0.01). Age ${\leq}65$, NLR>4, extrahepatic metastases and vascular invasion were all predictors of poorer overall survival. Multivariate analysis showed that NLR > 4, vascular invasion and extrahepatic metastases were independent predictors of poorer overall survival. The median TTP of patients with a high NLR was 2.5 months (95%CI, 1.4-3.6 months) compared with 4.5 months (95%CI, 3.9-5.1 months) for patients with a normal NLR (P=0.012). Conclusions: High baseline NLR was associated with worse OS and TTP for patients with advanced HCC treated with sorafenib.

Research Progress in Applying Proteomics Technology to Explore Early Diagnosis Biomarkers of Breast Cancer, Lung Cancer and Ovarian Cancer

Luo, Lu,Dong, Li-You,Yan, Qi-Gui,Cao, San-Jie,Wen, Xin-Tian,Huang, Yong,Huang, Xiao-Bo,Wu, Rui,Ma, Xiao-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

According to the China tumor registry 2013 annual report, breast cancer, lung cancer, and ovarian cancer are three common cancers in China nowadays, with high mortality due to the absence of early diagnosis technology. However, proteomics has been widespreadly implanted into every field of life science and medicine as an important part of post-genomics era research. The development of theory and technology in proteomics has provided new ideas and research fields for cancer research. Proteomics can be used not only for elucidating the mechanisms of carcinogenesis focussing on whole proteins of the tissue or cell, but also seeking the biomarkers for diagnosis and therapy of cancer. In this review, we introduce proteomics principles, covering current technology used in exploring early diagnosis biomarkers of breast cancer, lung cancer and ovarian cancer.