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Single-cell sequencing techniques from individual to multiomics analyses
Yukie Kashima,Yoshitaka Sakamoto,Keiya Kaneko,Masahide Seki,Yutaka Suzuki,Ayako Suzuki 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-
Here, we review single-cell sequencing techniques for individual and multiomics profiling in single cells. We mainly describe single-cell genomic, epigenomic, and transcriptomic methods, and examples of their applications. For the integration of multilayered data sets, such as the transcriptome data derived from single-cell RNA sequencing and chromatin accessibility data derived from single-cell ATAC-seq, there are several computational integration methods. We also describe single-cell experimental methods for the simultaneous measurement of two or more omics layers. We can achieve a detailed understanding of the basic molecular profiles and those associated with disease in each cell by utilizing a large number of single-cell sequencing techniques and the accumulated data sets.
Vision Based Pointing Device with Slight Body Movement
Motohiro Tanaka,Motoki Hara,Yoshitaka Morito,Taichi Sakamoto,Kazuya Miyamori,Shunji Moromugi,Takakazu Ishimatsu 제어로봇시스템학회 2008 제어로봇시스템학회 국제학술대회 논문집 Vol.2008 No.10
It is widely acknowledged that computer is powerful tool to improve the quality of life of the people with disability. One problem is how the user manipulates the computer using suitable input device designed for him or her. This paper proposes tow pointing devices based on vision for the people with disability as input devices. These devices detect the position of the marks attached on the users head. According to the head movements, computer cursor on the computer display is moved in two-dimensionally. A distinct advantage of these devices is that various strategies could be installed based on the situation of the users. The pointing device was applied to a drawing tool of the painting software for an patients successfully.