Study on the Mechanism of Fe, Al and Mn on Ginseng Rusty Root

XU YONGHUA 한국약용작물학회 2018 한국약용작물학회 학술대회논문집 Vol.2018 No.2

Application of the Multi-Source Data Fusion Algorithm in the Hail Identification

Yonghua Zhu,Yongqing Wang,Zhiqun Hu,Fansen Xu,Renqiang Liu 한국기상학회 2022 Asia-Pacific Journal of Atmospheric Sciences Vol.58 No.3

In this study, the canonical correlation analysis algorithm (CCA) is used to fuse the two-dimensional wind field retrieved from the single-Doppler weather radar, the three-dimensional wind field retrieved from the dual-Doppler weather radars, the observations from the ground automatic weather stations and the meteorological reanalysis data in three hail episodes (“0625” episode in Beijing, “0330” and “0801” episodes in Guangdong). During the hail episode in Beijing on June 25, 2020, an evident and long-lasting three-body scatter spike was observed, which played an important role in the hail identification and warning. In the three-dimensional wind field retrieved from the dual-Doppler weather radars, there is horizontal convergence of northeasterly and northwesterly winds and that of northwesterly and southeasterly winds in the low-level strong echo area, and there are obvious updrafts in the vertical wind field structure. Such a circulation configuration is favorable for the development and maintenance of hail storm. The multi-source data fusion of the wind fields can effectively improve the identification of the low-level convergence. The data fusion for the other two hail episodes (“0330” and “0801” episodes in Guangdong) yields the same conclusion. It is revealed that the dual-radar fusion performs better than the single-radar fusion in the identification of the meso-γ scale vortices. It can visually illustrate the characteristics of the cyclonic convergent flow fields which is more consistent with the near-surface observation. It can be concluded that the multi-source data fusion technique is practicable in the three severe convection processes.

A modal approach for the efficient analysis of a bionic multi-layer sound absorption structure

Yonghua Wang,Chengyu Xu,Yanling Wan,Jing Li,Huadong Yu,Luquan Ren 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.21 No.2

The interest of this article lies in the proposition of using bionic method to develop a new sound absorber and analyze the efficient of this absorber in a ski cabin. Inspired by the coupling absorption structure of the skin and feather of a typical silent flying bird — owl, a bionic coupling multi-layer structure model is developed, which is composed of a micro-silt plate, porous fibrous material and a flexible micro-perforated membrane backed with airspace. The finite element simulation method with ACTRAN is applied to calculate the acoustic performance of the multi-layer absorber, the vibration modal of the ski cabin and the sound pressure level (SPL) near the skier's ears before and after pasting the absorber at the flour carpet and seats in the cabin. As expected, the SPL near the ears was significantly reduced after adding sound-absorbing material. Among them, the model 2 and model 5 showed the best sound absorption efficiency and the SPL almost reduced 5 dB. Moreover, it was most effctive for the SPL reduction with full admittance configuration at both the carpet and the seats, and the carpet contribution seems to be predominant.

Panaxadiol saponins treatment caused the subtle variations in the global transcriptional state of Asiatic corn borer, Ostrinia furnacalis

Shuangli Liu,Yonghua Xu,Yugang Gao,Yan Zhao,Aihua Zhang,Liansheng Zang,Chunsheng Wu,Lianxue Zhang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.1

Background: The lepidopteran Asiatic corn borer (ACB), Ostrinia furnacalis (Guenee), has caused hugeeconomic losses throughout the Asian-Western Pacific region. Usually, chemical pesticides are used forthe control, but excessive use of pesticides has caused great harm. Therefore, the inartificial ecotypicpesticides to ACB are extremely essential. In our previous study, we found that panaxadiol saponins(PDS) can effectively reduce the harm of ACB by causing antifeedant activity. Therefore, it is necessary toreveal the biological molecular changes in ACB and the functionary mechanism of PDS. Methods: We analyzed the global transcription of ACB with different PDS concentration treatment (5 mg/mL, 10 mg/mL, and 25 mg/mL) by high-throughput sequencing and de novo transcriptome assemblymethod. Results: PDS treatment could cause the changes of many gene expressions which regulate its signalpathways. The genes in peroxisome proliferatoreactivated receptor (PPAR) signaling pathway weresignificantly downregulated, and then, the downstream fatty acid degradation pathway had also beengreatly affected. Conclusion: Through this experiment, we hypothesized that the occurrence of antifeedant action of ACBis because the PDS brought about the downregulation of FATP and FABP, the key regulators in the PPAR,and the downregulation of FATP and FABP exerts further effects on the expression of SCD-1, ACBP, LPL,SCP-X, and ACO, which leads to the disorder of PPAR signaling pathway and the fatty acid degradationpathway. Not only that, PDS treatment leads to enzyme activity decrease by inhibiting the expression ofgenes associated with catalytic activity, such as cytochrome P450 and other similar genes.

Adverse effects of subchronic exposure to cooking oil fumes on the gonads and the GPR30‑mediated signaling pathway in female rats

Xiaoyang Zhang,Yonghua He,Quanhui Lin,Lili Huang,Qunwei Zhang,Youqiong Xu 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.1

Background Cooking oil fumes (COFs) are composed of particulate matter, polycyclic aromatic hydrocarbons, volatile organic compounds, aldehydes, and ketones, and are currently a global health concern. Some agents in COFs are mutagenic and carcinogenic. However, only a few reports have addressed the hazardous effects of COF exposure on the female reproductive system. In this study, we explored the effects of subchronic exposure to COFs on female gonads in vivo and the possible involvement of the G-protein-coupled receptor 30 signaling pathway. Methods COFs were generated by heating commercially available canola oil in an iron pot. Adult female Wistar rats at 2 months of age were exposed to COFs at 32 mg/m3 for 0, 0.5, 1, 2, or 4 h/day for 56 days. The estrous cycle in rats was studied twice at 7:00 a.m. and 7:00 p.m. on the 43rd treatment day until the current estrous cycle was complete. The rat body weight was measured before the experiment and at day 56 post-exposure. At the end of the experiment, rat blood was collected for gonadal hormone assay, and ovaries were collected for histology and mRNA isolation. The mRNA levels of GPR30, EGFR, STAT3, and ERK were determined by quantitative RT-PCR. Results At a concentration of 32.21 ± 5.11 mg/m3, COF exposure extended the estrous cycle in rats, and ovary coefficient decreased. COFs showed various effects on the sex hormone levels and follicles, depending on its exposure level. Exposure to COFs led to the changes in mRNA levels of the G-protein-coupled receptor 30 (GPR30), epidermal growth factor receptor (EGFR), signal transducer and activator of transcription 3 (STAT3), and extracellular signal-regulated kinase (ERK). Conclusion This study indicated that cooking oil fume exposure disrupted the estrous cycle, sex hormone patterns, and follicle development in female rats in a dose-dependent manner. These adverse effects of cooking oil fumes on female reproductive health were correlated with the G-protein-coupled receptor 30-mediated signaling pathway.

Panaxadiol saponins treatment caused the subtle variations in the global transcriptional state of Asiatic corn borer, Ostrinia furnacalis

Liu, Shuangli,Xu, Yonghua,Gao, Yugang,Zhao, Yan,Zhang, Aihua,Zang, Liansheng,Wu, Chunsheng,Zhang, Lianxue The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.1

Background: The lepidopteran Asiatic corn borer (ACB), Ostrinia furnacalis (Guenee), has caused huge economic losses throughout the Asian-Western Pacific region. Usually, chemical pesticides are used for the control, but excessive use of pesticides has caused great harm. Therefore, the inartificial ecotypic pesticides to ACB are extremely essential. In our previous study, we found that panaxadiol saponins (PDS) can effectively reduce the harm of ACB by causing antifeedant activity. Therefore, it is necessary to reveal the biological molecular changes in ACB and the functionary mechanism of PDS. Methods: We analyzed the global transcription of ACB with different PDS concentration treatment (5 mg/mL, 10 mg/mL, and 25 mg/mL) by high-throughput sequencing and de novo transcriptome assembly method. Results: PDS treatment could cause the changes of many gene expressions which regulate its signal pathways. The genes in peroxisome proliferator-activated receptor (PPAR) signaling pathway were significantly downregulated, and then, the downstream fatty acid degradation pathway had also been greatly affected. Conclusion: Through this experiment, we hypothesized that the occurrence of antifeedant action of ACB is because the PDS brought about the downregulation of FATP and FABP, the key regulators in the PPAR, and the downregulation of FATP and FABP exerts further effects on the expression of SCD-1, ACBP, LPL, SCP-X, and ACO, which leads to the disorder of PPAR signaling pathway and the fatty acid degradation pathway. Not only that, PDS treatment leads to enzyme activity decrease by inhibiting the expression of genes associated with catalytic activity, such as cytochrome P450 and other similar genes.

Fault diagnosis of rotating machines based on modified hierarchical fluctuation dispersion entropy and multi-cluster feature selection

Baoyue Li,Yonghua Yu,Jia Hu,Bingxin Cao,Yangfeng Yao,Defeng Xu 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.12

The vibration signal of rotating mechanical equipment contains a large amount of information that can be used for the fault diagnosis of rotating mechanical equipment. However, the vibration information is distributed in multiple dimensions, and a single-scale analysis cannot effectively reflect its damage characteristics, reducing the accuracy of fault diagnosis. Accordingly, an improved hierarchical fluctuation dispersion entropy (MHFDE) method based on the improved hierarchical processing is proposed. MHFDE can simultaneously mine low- and highfrequency features in the time series, avoiding information omission. Comparison results of the simulated signals show that the proposed method has the advantages of high stability and accurate measurement of complexity. In combination with the multi-cluster feature selection (MCFS) and kernel limit learning machine (KELM) optimized by whale optimization algorithm (WOA), a rotating machinery damage recognition method based on MHFDE-MCFS and WOA-KEM was proposed. Three sets of typical rotating machinery datasets are used to verify the effectiveness of the proposed method. The results show that this method can not only accurately and stably identify the damage types of the three selected machinery but also have a higher accuracy of damage recognition compared with the existing feature extraction methods.

AC092127.1-miR-451a-AE binding protein 2 Signaling Facilitates Malignant Properties of Breast Cancer

Xiumei Zhang,Lin Cong,Dafang Xu,Qi Leng,Ming Shi,Yonghua Zhou 한국유방암학회 2021 Journal of breast cancer Vol.24 No.4

Purpose: The purpose of the current study was to explore the functions and potential mechanism of miR-451a in breast cancer (BC). Methods: Quantitative reverse transcription real-time polymerase chain reaction was used to analyze the expression of miR-451a in human normal mammary cells (MCF-10A) and BC cells. Colony formation assay, terminal-deoxynucleoitidyl transferase mediated nick end labeling assay and transwell assays were conducted to validate the effect of miR-451a on proliferation, apoptosis, migration and invasion of BC cells, respectively. RNA pull-down, RNA immunoprecipitation and luciferase reporter assays were applied to investigate the upstream and downstream mechanisms of miR-451a in BC cells. Results: MiR-451a was expressed at a low level in BC cells. Overexpression of miR-451a repressed BC cells proliferation, migration and invasion. Moreover, long non-coding RNA AC092127.1 acted as a sponge of miR-451a to enhance the expression level of AE binding protein 2 (AEBP2) that was demonstrated to be the target gene of miR-451a in BC cells. Finally, rescue experiments validated that miR-451a and AEBP2 involved in AC092127.1-mediated BC cell growth, migration and invasion. Conclusion: In a word, AC092127.1/miR-451a/AEBP2 axis contributes to BC cell growth, migration and invasion. Our results may help to find novel potential targets for BC treatment.

Amino acid, fatty acid, and carbohydrate metabolomic profiles with ginsenoside-induced insecticidal efficacy against Ostrinia furnacalis (Guenee)

Liu, Shuangli,Wang, Xiaohui,Zhang, Rui,Song, Mingjie,Zhang, Nanqi,Li, Wanying,Wang, Yingping,Xu, Yonghua,Zhang, Lianxue The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.4

Background: Previous studies have shown the insecticidal efficacy of ginsenosides. In the present study, we aimed to investigate the metabolic mechanism related to the inhibitory effect of panaxadiol saponins (PDSs) against the Asian corn borer Ostrinia furnacalis (Guenee). Methods: Third instar larvae of O. furnacalis were fed normal diets with different concentrations of PDSs for 4 days. The consumption index, relative growth rate, approximate digestibility, and conversion of ingested and digested food were recorded. A targeted gas chromatographye-mass spectrometry assay was performed to detect the profiles of amino acids, fatty acids, and carbohydrates in larvae of O. furnacalis. In addition, the activity of detoxification-related enzymes was determined. Results and Conclusions: PDSs decreased the consumption index, relative growth rate, approximate digestibility, and conversion of ingested and digested food in the 3rd instar larvae of O. furnacalis in a dose-dependent manner. PDSs decreased 15 free amino acids, 16 free fatty acids, and 5 carbohydrates and increased the levels of palmitoleic acid, palmitic acid, and 9-octadecenoic acid in the 3rd instar larvae. The activity of detoxification-related enzymes, such as acetylcholinesterase, glutathione S-transferase, cytochrome P450, carboxylesterase, trehalase, acid phosphatase, and alkaline phosphatase, was reduced in a dose-dependent manner in the 3rd instar larvae exposed to PDSs. These data confirmed the inhibitory effect of PDSs against growth, food utilization, and detoxification in the 3rd instar larvae of O. furnacalis and the potential for using PDSs as an efficient tool for insect pest management for O. furnacalis larvae.

Amino acid, fatty acid, and carbohydrate metabolomic profi les with ginsenoside-induced insecticidal effi cacy against Ostrinia furnacalis (Guenee)

Shuangli Liu,Xiaohui Wang,Rui Zhang,Mingjie Song,Nanqi Zhang,Wanying Li,Yingping Wang,Yonghua Xu,Lianxue Zhang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.4

Background: Previous studies have shown the insecticidal efficacy of ginsenosides. In the present study,we aimed to investigate the metabolic mechanism related to the inhibitory effect of panaxadiol saponins(PDSs) against the Asian corn borer Ostrinia furnacalis (Guenee). Methods: Third instar larvae of O. furnacalis were fed normal diets with different concentrations of PDSsfor 4 days. The consumption index, relative growth rate, approximate digestibility, and conversion ofingested and digested food were recorded. A targeted gas chromatographyemass spectrometry assaywas performed to detect the profiles of amino acids, fatty acids, and carbohydrates in larvae ofO. furnacalis. In addition, the activity of detoxification-related enzymes was determined. Results and Conclusions: PDSs decreased the consumption index, relative growth rate, approximate digestibility,and conversion of ingested and digested food in the 3rd instar larvae of O. furnacalis in a dosedependentmanner. PDSs decreased 15 free amino acids, 16 free fatty acids, and 5 carbohydrates andincreased the levels of palmitoleic acid, palmitic acid, and 9-octadecenoic acid in the 3rd instar larvae. The activity of detoxification-related enzymes, such as acetylcholinesterase, glutathione S-transferase,cytochrome P450, carboxylesterase, trehalase, acid phosphatase, and alkaline phosphatase, was reducedin a dose-dependent manner in the 3rd instar larvae exposed to PDSs. These data confirmed theinhibitory effect of PDSs against growth, food utilization, and detoxification in the 3rd instar larvae ofO. furnacalis and the potential for using PDSs as an efficient tool for insect pest management forO. furnacalis larvae.