The causal deletions in the second exon of An-3 closely associated with awn development and rice yield

Lilong Liu,Baoxiang Qin,Yunzhen Li,Minyi Wei,Zishuai Wu,Fang Liu,Yongfu Qiu,Jijing Luo,Rongbai Li 한국유전학회 2017 Genes & Genomics Vol.39 No.11

Awn is one of important traits during rice domestication. To understand the development of rice awn and the roles it played in rice domestication, we preliminary mapped a major QTL An-3 for awn development using chromosome segment substitution line CSSL138 developed by introgressed genomic fragments of long-awned Guangxi common wild rice (GXCWR, Oryza rufipogon Griff.) into genetic background of short-awned indica cultivar 93–11. An-3 was then fine mapped to a 7-kb region of chromosome 8. An epidermal patterning factor-like protein gene was identified as the single candidate gene corresponding to this QTL. An-3 was showed to be an allele of RAE2 and GAD1, and negatively regulated 1000-grains weight, grain length, and length–width ratio. Comparing with the coding sequences of An-3 from CSSL138, a 2- and 4-bp frameshift deletions in the second exon were identified in 93–11 and Nipponbare, respectively. Taken together, our results provide valuable natural variation in the alleles of An-3 between common wild rice and cultivated rice, which will be helpful in clarifying the mechanism of awn development and promoting the application of an-3 in genetic improvement of rice yield traits.

Development and application of EST-SSR to evaluate the genetic diversity of Southeast Asian rice planthoppers

Yongfu Qiu,Xiaozhen Jiao,Dehui Hu,Fang Liu,Fengkuan Huang,Rongbai Li 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3

A total of 320 ESTs (Expressed Sequence Tags, 12.8%) of rice planthoppers were identified to carry SSR (Simple Sequence Repeat) motifs from2491 ESTs downloaded frompublic database, and 397 pairs of brown planthopper (BPH; Nilaparvata lugens Stål) and 25 pairs ofwhite-backed planthopper (WBPH; Sogatella furcifera) (Hemiptera: Delphacidae) EST-SSR were detected based on the motifs. The dinucleotide repeat was the dominant type with repeat motif AG/GA/TC/CT; and the trinucleotide repeat was the style of AAG/AGA/GAA/TTC/TCT/CTT among the BPH repeat motifs. A total of 20 EST-SSRs (16 developed in the present study and four fromprevious studies) were applied to survey the genetic diversity of the 15 rice planthoppers. Consequently, 18 pairs of primer amplifies clear, dominant and polymorphic gel bands among the collected planthoppers population; one ESTSSR amplified a single gel band; and one had no result. Furthermore, a total of 227 bands were produced by the selected 19 pairs of primers, of which 207 bands (91.2%) were polymorphic among the test insect populations. Genetic diversity analysis suggested that the BPH populations from Cuu Long, Thailand and Ho Chi Minh were clustered, respectively; and Hue and Long Xuyen were composed into one class; the others were clustered together when the similar coefficient was 0.76. The results show that it is an effective and available approach to develop SSR markers based on ESTs in rice planthoppers; and also the developed EST-SSR markers could detect the genetic diversity of the planthoppers population, which would be beneficial for rice planthoppers control strategy. ©

STRONG CONVERGENCE OF HYBRID METHOD FOR ASYMPTOTICALLY NONEXPANSIVE MAPPINGS AND SEMIGROUPS

Liu, Li,Wang, Lijing,Su, Yongfu The Korean Society for Computational and Applied M 2011 Journal of applied mathematics & informatics Vol.29 No.3

In this paper, some strong convergence theorems are obtained for hybrid method for modified Ishikawa iteration process of asymptotically nonexpansive mappings and asymptotically nonexpansive semigroups in Hilbert spaces. The results presented in this article generalize and improve results of Tae-Hwa Kim and Hong-Kun Xu and others. The convergence rate of the iteration process presented in this article is faster than hybrid method of Tae-Hwa Kim and Hong-Kun Xu and others.

STRONG CONVERGENCE OF HYBRID METHOD FOR ASYMPTOTICALLY NONEXPANSIVE MAPPINGS AND SEMIGROUPS

Li Liu,LijingWang,Yongfu Su 한국전산응용수학회 2011 Journal of applied mathematics & informatics Vol.29 No.3

In this paper, some strong convergence theorems are obtained for hybrid method for modified Ishikawa iteration process of asymptotically nonexpansive mappings and asymptotically nonexpansive semigroups in Hilbert spaces. The results presented in this article generalize and improve results of Tae-Hwa Kim and Hong-Kun Xu and others. The convergence rate of the iteration process presented in this article is faster than hybrid method of Tae-Hwa Kim and Hong-Kun Xu and others.

Research of Maize Seeds Classification Recognition Based on the Image Processing

Dongming Li,Yongfu Liu,Liang Gao 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.11

The maize seeds recognition is an important aspect of the seed quality testing and breeding. With the development of computer technology, it is an inevitable tendency to recognize seeds varieties and inspect quality using machine vision instead of human vision. For the characteristics of maize seeds and their images, seeds varieties recognition technology and algorithm was studied and explored in depth and their shape and color feature parameters were extracted. Based on 4 species including Nongda 108, Zhengdan 958, Ludan 981 and Jingdan 28, the classifier was designed using BP neural network to do varieties recognition testing. Research showed the average recognition accuracy was more than 94.5%, and that recognition and detection of maize seeds varieties based on the image technology is feasible.

Gold Price Prediction Method Based on Improved PSO-BP

Yan Wang,Liguo Zhang,Yongfu Liu,Jun Guo 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.11

Aimed at the highly nonlinear and uncertainty of gold price changes, a new method for gold price predition based on improved PSO-BP is proposed. By introducing mutation operation and adaptive adjust of inertia weight , the problem of easy to fall into local optimum, premature, low precision and low later interation efficiency of PSO are solved. By using the improved PSO to optimiaze BP neural network’s parameters, the learning rate and optimization capability of conventional BP are effectively improved. The simulation results of gold price prediction show that the predict accuracy of the new method is significantly higher than that of conventional BP neural network and wavelet neural network method. And the method is effective and feasible.

Dynamical Expression of MicroRNA-127-3p in Proliferating and Differentiating C2C12 Cells

Li, Jie,Wang, Gaofu,Jiang, Jing,Zhou, Peng,Liu, Liangjia,Zhao, Jinhong,Wang, Lin,Huang, Yongfu,Ma, Youji,Ren, Hangxing Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.12

MicroRNAs (miRNAs) are highly conserved, short non-coding RNAs that regulate gene expression at the posttranscriptional level. Although many miRNAs are identified in muscles and muscle cells, their individual roles are still not fully understood. In the present study, we investigated a muscle highly-expressed miRNA, miR-127-3p, in C2C12 myoblasts and tissues of goats with different muscle phenotypes (Boer vs Wushan black goats). Our results demonstrated that i) miR-127-3p was extensively expressed in tissues of goats; ii) miR-127-3p was higher expressed in muscle, spleen, heart, and skin in the muscular goats (Boer goats) than the control (Wushan black goats). Then we further characterized the dynamical expression of miR-127-3p, MyoD, MyoG, Myf5, Mef2c, and Myosin in the proliferating and differentiating C2C12 myoblasts at day of 0, 1, 3, 5, and 7 in culture mediums. Especially, we found that miR-127-3p was significantly higher expressed in the proliferating than differentiating cells. Our findings suggest that miR-127-3p probably plays roles in the proliferation and differentiation of myoblasts, which further underlies regulation of muscle phenotype in goats.

Reliability optimization design of hydraulic system considering oil contamination

Xinda Zhou,Zhaojun Yang,Hailong Tian,Chuanhai Chen,Liding Wang,Yongfu Zhu,Jia Liu 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.12

This paper presents a reliability optimization design method for a hydraulic system that considers oil contamination. The proposed method applies the quantitative relationship between oil contamination and system reliability to the reliability design of a hydraulic system, while considering the performance, layout, and other design factors. A global reliability model of the hydraulic system is constructed based on the oil contamination control model. Further studies aiming at an optimization design model of the hydraulic system is established where the cost is the objective function, reliability index, size and structure range, performance requirements are constraints, structure size and oil change period are design variables. For illustration, a case study on the reliability optimization design of the oil supply point branch of the static pressure bracket of a certain type of heavy CNC horizontal lathe is considered.