Hydrochar preparation from black liquor by CO2 assisted hydrothermal treatment: Optimization of its performance for Pb2+ removal

Yong Sun,Jing Ping Zhang,Fei Guo,Lian Zhang 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.9

Hydrochar was produced from hydrothermal treatment of corn straw black liquor. Response surface methodology (RSM) and the central composite design (CCD) were employed for determination of optimal char with maximum Pb2+ removal capacity. The operational parameters such as hydrothermal temperature (oC), duration (min) and solid liquid ratio (LSR) were chosen as independent variables in CCD. The statistical analysis indicates that the effects of hydrothermal temperature, duration, LSR and combined effect of hydrothermal temperature and duration are all significant for the Pb2+ removal capacity. The optimal condition for achieving the maximum Pb2+ adsorption capacity is obtained as the following: hydrothermal temperature (205 oC), duration (28min), LSR (12) with Pb2+ removal capacity reaching 47mg/g. The BET specific surface area of char prepared at optimal conditions could reach 85m2/g.

Insect-specific microRNA involved in the development of the silkworm Bombyx mori

Yong Zhang,Xue Zhou,Xie Ge,Jiang-Hao Jiang,Mu-Wang Li,Shi-Hai Jia,Xiao-Nan Yang,Yun-Chao Kan,Xue-Xia Miao,Guo-Ping Zhao,Fei Li,Yong-Ping Huang 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

MicroRNAs (miRNAs) are endogenous non-coding genes that participate in post-transcription regulation by either degrading mRNA or blocking its translation. It is considered to be very important in regulating insect development and metamorphosis. Insects are the largest group of animals and are extremely valuable in biological and agriculture research. Insects are also important pests to human health and agriculture, and efforts are necessary protect both humans and plants from disease and damage. Despite their importance, insects lag behind mammals, nematodes, and plants in miRNA research. At present, only 279 insect miRNAs have been identified from Drosophila melanogaster, Anopheles gambiae, Apis mellifera, Bombyx mori, and D. pseudoobscura in miRBase, and most of these miRNAs were computationally predicted without experimental validation. Functional analysis of insect miRNAs has only been conducted in D. melanogaster.

A Hierarchical Single-Surface Model for an Unsaturated Soil-Structure Interface

Fei Peng,Xin Li,Meng-fan Lv,Yong-Hui Li,Yuan-Cheng Guo 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.6

In this paper, according to the shear test of the interface between unsaturated soil and steel plate, the modified apparent roughness is used to describe interface roughness. And considering the influence of matrix suction and interface roughness, the stress variable is constructed to improve the yield and plastic potential functions. Then a modified hierarchical single-surface elastoplastic interface constitutive model (HISS-Unsaturated-Int model) is set up. The finite element calculation of HISS-Unsaturated-Int model is achieved by compiling and calling the user subroutine FRIC of ABAQUS. The interface shear test between unsaturated soil and structure with different water contents and the pull-out shear test between unsaturated soil and soil nail are simulated. The calculation results are consistent with those of experiments, which shows the modified interface constitutive model can effectively predict the shear mechanical properties of soil-structure interface under different soil moisture content, normal stress and interface roughness.

An Improved CPTu-based Method to Estimate Jacked Pile Bearing Capacity and Its Reliability Assessment

Yong-hong Miao,Ping-ping Zuo,Jie Yin,Shoaib Ahmed,Guo-long Bai,Jian-fei Lu 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.9

This paper presents an improved method for predicting the bearing capacity of single jacked pile based on piezocone penetration test (CPTu) data which contain tip resistance (qt), side friction ( fs) and excess dynamic pore water pressure (u2). Firstly, the average value of qt was determined to calculate the pile unit base resistance according to the influence zone of the logarithmic spiral concerning the effect of soil types. Secondly, fs and u2 were used to calculate the pile unit shaft resistance considering the effect of friction fatigue. The pile jacking force was obtained in terms of unit base resistance and unit shaft resistance. The ultimate bearing capacity was finally determined using pile jacking force multiplied by a force ratio denoted as Rf. The ultimate bearing capacities calculated for two case projects show a good agreement with the measured results, which indicates that the proposed method is feasible and effective. Reliability assessment was carried out on the proposed method and other five estimation methods with respect to the reliability index obtained by advanced first-order second-moment (AFOSM) method. Comparison result showed that the method proposed in this study has a higher reliability index over other five existing methods. Therefore, the proposed method can be used as a reliable alternative to predict the bearing capacity in the engineering practice of jacked pile foundation.

Durability Model of Exposed Concrete On-Site Based on Dual Coupling Factors

Yong Fu,Hongxia Qiao,Chenggong Lu,Theogene Hakuzweyezu,Fei Guo 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.1

Aiming at the problem that the reliability analysis of the outdoor exposed concrete structure under the action of a single factor is not consistent with the actual service environment, a reliability analysis based on the Copula function describing multiple degradation factors is proposed. According to the actual data collected on-site, the relative quality evaluation parameters (RQEP) and relative dynamic elastic modulus evaluation parameters (RDEMEP) was selected as the key degradation indicators, and the Aperture Information Criterion (AIC) was used to determine the optimal marginal distribution function in different degradation trajectories, and obtain the most optimal edge distribution function under a single factor. Optimize the edge distributions reliability curve, establish the relationship between the edge distributions through the Copula function, and couple the edge distributions of the remaining life of the concrete to obtain the joint reliability function of the concrete under the action of the coupling factors. The results show that the selection of two degradation indicators, RQEP and RDEMEP, is helpful in establishing the concrete life prediction model in the field exposure environment, and the RDEMEP are more sensitive to the field exposure environment than the RQEP. The Gumbel Copula distribution function can reasonably predict the reliability of exposed concrete on-site under dual coupling factors. The service life of C40 and C50 exposed concrete on site is about 400 and 550 months, respectively.

Structural Damage Identification Based on Convolutional Neural Network Group Considering the Sensor Fault

Yong-Peng Luo,Linkun Wang,Xu Guo,Jinlin Zheng,Fei-Yu Liao,Zixiong Guo 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8

This article proposes a structural damage identification method based on one-dimensional convolutional neural network group considering sensor faults. The method aims to reduce the damage misjudgment caused by sensor faults. In the proposed method, according to the sensor layout, some convolutional neural network sub-models are established to extract the features from raw vibration data for sensor fault diagnosis and structural damage identification; then two convolutional neural networks groups, namely the sensor fault diagnosis group and the damage identification group are designed on the basis of the functions of each sub-model. The sensor fault diagnosis group determines whether the sensor data is abnormal and truncates the abnormal signal. The remaining normal signal are entered into the damage identification group and the final damage identification results are calculated according to the statistical decision module. The effectiveness of the devised method is verified by the IASC–ASCE benchmark structure and laboratory experiments. The results demonstrate that the sensor fault diagnosis and damage identification accuracy of each sub-model ranges from 98.54% to 99.77% and from 87.21% to 91.74% respectively at different noise levels; the damage identification group can reduce the impact of sub-model misjudgment on the structural damage identification. The accuracy of the final damage identification results is 100%. The identification time of all samples in the test set is 53.09 s and 22.93 s, respectively, for SHM benchmark and Laboratory experiment cases. And the average judgment time of each submodel in the sensor fault diagnosis group was 278 and 94 ms, and that of each submodel in the damage identification group was 294 and 105 ms, respectively, for a single test sample, which fulfills the requirements of online damage identification for structural health monitoring.

Application of Joint Detection of AFP, CA19-9, CA125 and CEA in Identification and Diagnosis of Cholangiocarcinoma

Li, Yong,Li, Da-Jiang,Chen, Jian,Liu, Wei,Li, Jian-Wei,Jiang, Peng,Zhao, Xin,Guo, Fei,Li, Xiao-Wu,Wang, Shu-Guang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Objective: To explore the application of joint detection of serum AFP, CA19-9, CA125 and CEA in identification and diagnosis of cholangiocarcinoma (CC). Materials and Methods: The levels of serum AFP, CA19-9, CA125 and CEA of both 30 patients with CC and 30 patients with hepatocellular carcinoma (HCC) were assessed. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic effects of single and joint detection of those 4 kinds of tumor markers for CC. Results: The levels of serum CA19-9, CA125 and CEA in CC patients were higher than that in HCC patients,whereas that of serum AFP was significantly lower s. The area under ROC curve of single detection of serum AFP, CA19-9, CA125 and CEA were 0.05, 0.86, 0.84 and 0.83, with the optimal cutoff values of 15.4 ng/ml, 125.1 U/ml, 95.7 U/ml and 25.9 ng/ml, correspondingly, and the percentage correct single diagnosis was <79%. With joint detection, the diagnostic effect of combined AFP, CA19-9, CA125 and CEA was the highest, with an area under the ROC curve of 0.94 (95%CI 0.88~0.99). Conclusions: Single detection of serum CA19-9, CA125 and EA is not meaningful. The sensitivity, specificity, the rate of correct diagnosis and the area under ROC curve of joint detection of AFP, CA19-9, CA125 and CEA are highest, indicating that the joint detection of these 4 tumor markers is of great importance in the diagnosis of CC.

Expression of Lamininγ2 in Extrahepatic Cholangiocarcinoma Tissues and its Influence on Tumor Invasion and Metastasis

Li, Yong,Li, Da-Jiang,Chen, Jian,Liu, Wei,Li, Jian-Wei,Jiang, Peng,Zhao, Xin,Guo, Fei,Li, Xiao-Wu,Wang, Shu-Guang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Objective: To explore the expression of $laminin{\gamma}2$ in extrahepatic cholangiocarcinoma (EHCC) tissues and its influence on tumor invasion and metastasis. Materials and Methods: Paraffin embedding samples of cancer, para-cancer, lymph node metastatic and hepatic metastatic tissues from 79 patients undergoing EHCC resection were collected. Expression of $laminin{\gamma}2$ was detected by immunohistochemistry and its relationship with clinical pathological characteristics and the prognosis of EHCC patients were analyzed. Results: $Laminin{\gamma}2$ showed negative staining in para-cancer tissues, but demonstrated a 51.9% (41/79) positive expression rate in extracellular matrix (ECM) or cytoplasm of EHCC tissues. In lymph node metastatic and distant metastatic nidi, expression of $laminin{\gamma}2$ was significantly higher than in the primary nidi (${\chi}^2=7.4173$, P=0.0065; ${\chi}^2=4.0077$, P=0.0453). The expression was in obvious association with lymph node metastasis (P<0.01), but had no relevance with age, gender, tumor location, tumor stage, differentiation and distant metastasis in ECM (P>0.05), whereas it was in marked connection with lymph node and distant metastasis (P<0.05 or P<0.01), but had no relationship with age, gender, tumor location, tumor stage and differentiation in cytoplasm (P>0.05). However, the median survival time and median recurrent period of patients with positive expression of $laminin{\gamma}2$ in both cytoplasm and ECM of tumor cells, only in ECM and only in cytoplasm, were evidently lower than with negative expression of $laminin{\gamma}2$ in RCM and cytoplasm (P<0.05 or P<0.01). Further Cox regression analysis showed that the positive expression of $laminin{\gamma}2$ and the tumor differentiation were independent risk factors influencing the prognosis of EHCC patients. Conclusions: Abnormal expression of $laminin{\gamma}2$ may be closely associated with invasion and metastasis of tumor cells, and thus a potential molecular marker for prognosis of EHCC patients.

Human lactoferrin efficiently targeted into caprine beta-lactoglobulin locus with transcription activator-like effector nucleases

Yu-Guo Yuan,Shao-Zheng Song,Meng-Ming Zhu,Zheng-Yi He,Rui Lu,Ting Zhang,Fei Mi,Jin-Yu Wang,Yong Cheng 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.8

Objective: To create genetically modified goat as a biopharming source of recombinant human lacotoferrin (hLF) with transcription activator-like effector nucleases. Methods: TALENs and targeting vector were transferred into cultured fibroblasts to insert hLF cDNA in the goat beta-lactoglobulin (BLG) locus with homology-directed repair. The gene targeted efficiency was checked using sequencing and TE7I assay. The bi-allelic gene targeted colonies were isolated and confirmed with polymerase chain reaction, and used as donor cells for somatic cell nuclear transfer (SCNT). Results: The targeted efficiency for BLG gene was approximately 10%. Among 12 Bi-allelic gene targeted colonies, five were used in first round SCNT and 4 recipients (23%) were confirmed pregnant at 30 d. In second round SCNT, 7 (53%), 4 (31%), and 3 (23%) recipients were confirmed to be pregnant by ultrasound on 30 d, 60 d, and 90 d. Conclusion: This finding signifies the combined use of TALENs and SCNT can generate bi-allelic knock-in fibroblasts that can be cloned in a fetus. Therefore, it might lay the foundation for transgenic hLF goat generation and possible use of their mammary gland as a bioreactor for large-scale production of recombinant hLF.

Glucose and Insulin Stimulate Lipogenesis in Porcine Adipocytes: Dissimilar and Identical Regulation Pathway for Key Transcription Factors

Zhang, Guo Hua,Lu, Jian Xiong,Chen, Yan,Dai, Hong Wei,ZhaXi, YingPai,Zhao, Yong Qing,Qiao, Zi Lin,Feng, Ruo Fei,Wang, Ya Ling,Ma, Zhong Ren Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.11

Lipogenesis is under the concerted action of ChREBP, SREBP-1c and other transcription factors in response to glucose and insulin. The isolated porcine preadipocytes were differentiated into mature adipocytes to investigate the roles and interrelation of these transcription factors in the context of glucose- and insulin-induced lipogenesis in pigs. In ChREBP-silenced adipocytes, glucose-induced lipogenesis decreased by ~70%, however insulin-induced lipogenesis was unaffected. Moreover, insulin had no effect on ChREBP expression of unperturbed adipocytes irrespective of glucose concentration, suggesting ChREBP mediate glucose-induced lipogenesis. Insulin stimulated SREBP-1c expression and when SREBP-1c activation was blocked, and the insulin-induced lipogenesis decreased by ~55%, suggesting SREBP-1c is a key transcription factor mediating insulin-induced lipogenesis. $LXR{\alpha}$ activation promoted lipogenesis and lipogenic genes expression. In ChREBP-silenced or SREBP-1c activation blocked adipocytes, $LXR{\alpha}$ activation facilitated lipogenesis and SREBP-1c expression, but had no effect on ChREBP expression. Therefore, $LXR{\alpha}$ might mediate lipogenesis via SREBP-1c rather than ChREBP. When ChREBP expression was silenced and SREBP-1c activation blocked simultaneously, glucose and insulin were still able to stimulated lipogenesis and lipogenic genes expression, and $LXR{\alpha}$ activation enhanced these effects, suggesting $LXR{\alpha}$ mediated directly glucose- and insulin-induced lipogenesis. In summary, glucose and insulin stimulated lipogenesis through both dissimilar and identical regulation pathway in porcine adipocytes.