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Asami Yonekura,Hiroharu Kawanaka,V. B. Surya Prasath,Bruce J. Aronow,Haruhiko Takase 대한의용생체공학회 2018 Biomedical Engineering Letters (BMEL) Vol.8 No.3
In the field of computational histopathology, computer-assisted diagnosis systems are important in obtaining patientspecificdiagnosis for various diseases and help precision medicine. Therefore, many studies on automatic analysis methodsfor digital pathology images have been reported. In this work, we discuss an automatic feature extraction and disease stageclassification method for glioblastoma multiforme (GBM) histopathological images. In this paper, we use deep convolutionalneural networks (Deep CNNs) to acquire feature descriptors and a classification scheme simultaneously. Further,comparisons with other popular CNNs objectively as well as quantitatively in this challenging classification problem isundertaken. The experiments using Glioma images from The Cancer Genome Atlas shows that we obtain 96:5% averageclassification accuracy for our network and for higher cross validation folds other networks perform similarly with a higheraccuracy of 98:0%. Deep CNNs could extract significant features from the GBM histopathology images with highaccuracy. Overall, the disease stage classification of GBM from histopathological images with deep CNNs is verypromising and with the availability of large scale histopathological image data the deep CNNs are well suited in tacklingthis challenging problem.
Hiroshi Yonekura,Shinya Kanazawa,Ikuko Miyawaki,Kazuo Yamazaki 대한마취통증의학회 2014 Korean Journal of Anesthesiology Vol.67 No.1
Unroofed coronary sinus (URCS) is a rare cardiac anomaly, in which communication occurs between the coronary sinus (CS) and the left atrium (LA) because of partial or complete absence of the CS roof. A 30-year-old woman was scheduled for surgical closure of atrial septal defect, mitral valve repair and tricuspid annuloplasty. The intraoperative transesophageal echocardiography (TEE) revealed left-to-right shunt between the CS and the LA. The three-dimensional (3D) TEE confirmed the diagnosis of partially URCS. This defect was repaired with a pericardial patch. In this case, the 3D images of URCS, which were a helpful supplement to the 2D images, providing better visualization of the wall defect and more information regarding the size and location of the defect. The combined use of 2D and 3D images provides valuable information to aid in understanding the anatomy and morphology of this rare anomaly.
VRP(Vascular Rab-GAP/TBC Domain Containing Protein) 과발현이 혈관내피세포의 기능에 미치는 영향
김철희,Hideto Yonekura,Hiroshi Yamamoto 대한당뇨병학회 2003 Diabetes and Metabolism Journal Vol.27 No.6
-Background: VRP(vascular Rab-GAP/TBC domain containing protein) is a recently discovered gene from antisense-display screening of angiogenesis- related genes. However, its function in vascular endothelial cells has not been elucidated yet. This study was performed to examine the effects of overexpression of VRP on the function of vascular endothelial cells.Methods: VRP cDNA was cloned from polyA+ RNA from human microvascular endothelial cells, and inserted into a mammalian expression plasmid vector under the control of a CMV promotor. The constructed VRP expression vector was transfected into ECV304 cells. Then the proliferation, tube formation, and vascular endothelial growth factor(VEGF) secretion of the VRP-overexpressed cells were examined.Results: The expression of VRP mRNA was about two-fold greater in the VRP- transfected cells than in the control ECV304 cells. There was, however, no significant difference in the proliferation, cord-like structure formation, and VEGF secretion between the two cell groups.Conclusion: These results demonstrate that VRP overexpression does not affect the proliferation, tube formation, or VEGF secretion of ECV304 cells. Further studies are needed to elucidate the functional role of VRP in endothelial cells(J Kor Diabetes Assoc 27:449~455, 2003). 연구배경: 최근 혈관신생에 관련된 유전자에 대한 antisense-display 방법에 의한 검색으로 VRP(vascular Rab-GAP/TBC domain containing protein) 유전자가 발견되었으나, 그 기능은 아직 밝혀져 있지 않다. 본 연구자들은 혈관 내피세포의 mRNA로부터 VRP cDNA를 클로닝하고, 이를 혈관내피세포에 과발현시켜 그 기능을 살펴보고자 하였다.방법: 사람 미세혈관 내피세포의 mRNA로부터 VRP cDNA를 클로닝하고, 이를 CMV promotor의 조절하에 과발현하도록 하는 플라스미드 벡터를 제작하였다. 이 벡터를 ECV304 혈관내피세포주에 transfection시킨 후, 세포의 증식, tube 형성, 혈관내피세포 증식인자(VEGF) 분비에 미치는 영향을 원래의 세포주와 비교하였다.결과: VRP 과발현 세포들은 대조군 세포에 비하여 약 2배 VRP mRNA 발현이 증가하였다. 그러나 VRP를 과발현 시킨 ECV304 세포는 원래의 세포주에 비하여 증식, tube 형성, VEGF 분비에 뚜렷한 차이가 관찰되지 않았다.