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- 저자 : Yibing Huang (검색결과 4 건)
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Huang Jiazhen,Wei Wei,Kang Fuli,Tan Shuang,Li Yibing,Lu Xiaohang,Wang Ning 한국유전학회 2023 Genes & Genomics Vol.45 No.12
Background Cervical cancer, as one of the most common cancers in women, remains a major health threat worldwide. Annexin A3 (ANXA3), a component of the annexin family, is upregulated in numerous cancers, with no explicit role in cervical cancer. Objective This study aims to investigate the function of ANXA3 in cervical cancer. Methods Differential expression genes between the cervical cancer tissues of patients and the controls were analyzed in The Cancer Genome Atlas (TCGA) and Gene Expression Profiling Interactive Analysis (GEPIA) database. Using transfection approaches to either upregulate or downregulate ANXA3, its role in cell proliferation and chemosensitivity of human cervical cancer cell lines (HeLa and C33A) was evaluated. Furthermore, the binding activity between YAP1 and ANXA3 was also explored. Results Genomics analysis indicated that differential genes were mostly associated with cell cycle progression and DNA replication. ANXA3 was highly expressed in the cervical cancer tissues and closely linked to malignancy degree. Knockdown of ANXA3 in cervical cancer cells inhibited cell cycle progression. A similar result was observed in the reduction of cyclin D, CDK4, cyclin E, and CDK2 in cervical cancer cells with ANXA3 silencing. Cervical cancer cells obtained high sensitivity to cisplatin (DDP) when ANXA3 was downregulated. Conversely, these capabilities were the opposite in cervical cancer cells overexpressing ANXA3. Furthermore, the expression levels of ANXA3 and YAP1 were positively correlated. YAP1 upregulation was positively connected with malignant behaviors, which were reversed by ANXA3 downregulation. Conclusion In light of our findings, targeting ANXA3 expressed in cervical cancer might contribute to more potential therapeutic strategies.
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Development and characterization of simple sequence repeat (SSR) markers based on a full-length c
Jia Wang,Yibing Huang,Zhitong Chen,Surong Jin,Zhongli Hu,Ying Diao 한국유전학회 2017 Genes & Genomics Vol.39 No.12
Napier grass (Pennisetum purpureum Schum.) is an economically important species used as a fodder crop, reflecting its high biomass, perennial nature and pest resistance. In order to understand the genomic information and development of this species based on SSR markers, the transcriptome for Napier grass was obtained using nextgeneration sequencing. A total of 117,076 sequence contigs were obtained, with lengths of 201–10,652 bp. Among these contigs, 44,313 (37.85%) sequences were annotated against public protein databases (E-value <10−5). In addition, the contigs generated from the transcript sequencing were also analysed for the presence of simple sequence repeats (SSRs). A total of 12,744 SSR motifs were identified with in these contigs and corresponding primer pairs were designed. Empirical validation of a cohort of 200 SSRs was performed, with 34% being polymorphic for 20 Napier grass accessions. The polymorphic index content values for each primer ranged from 0.105 to 0.749, with an average of 0.409. The development of genetic, genomic resources and EST-SSRs for Napier grass will contribute to novel gene discovery and the marker-assisted selective breeding of this species.
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Hongmei Xu,Libin Wang,Jian Huang,YanQing Zhang,Feng Ma,Jianmin Wang,WenChun Xu,XueMei Zhang,YiBing Yin,KaiFeng Wu 한국미생물학회 2015 The journal of microbiology Vol.53 No.2
Pneumococcal asymptomatic colonization of the respiratory tracts is a major risk for invasive pneumococcal disease. We have previously shown that pneumococcal wall teichoic acid (WTA) was involved in pneumococcal infection of sepsis and adherence to epithelial and endothelial cells. In this study, we investigated the contribution of pneumococcal WTA to bacterial colonization and dissemination in murine models. The result showed that nasopharynx colonizing D39 bacterial cells have a distinct phenotype showing an increased exposure of teichoic acids relative to medium-grown bacteria. The WTA-deficient mutants were impaired in their colonization to the nasopharynx and lungs, and led to a mild inflammation in the lungs at 36 h post-inoculation. Pretreatment of the murine nares with WTA reduced the ability of wild type D39 bacteria to colonize the nasopharynx. In addition, the WTA-deficient strain was impaired in its ability to invade the blood and brain following intranasal administration. WTA-deficient D39 strain was reduced in C3 deposition but was more susceptible to the killing by the neutrophils as compared with its parent strain. Our results also demonstrated that the WTA enhanced pneumococcal colonization and dissemination independently of the host strains. These results indicate that WTA plays an important role in pneumococcal pathogenesis, both in colonization and dissemination processes.
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