ANALYSIS OF SOME PROJECTION METHODS FOR THE INCOMPRESSIBLE FLUIDS WITH MICROSTRUCTURE

Jiang, Yao-Lin,Yang, Yun-Bo Korean Mathematical Society 2018 대한수학회지 Vol.55 No.2

In this article, some projection methods (or fractional-step methods) are proposed and analyzed for the micropolar Navier-Stokes equations (MNSE). These methods allow us to decouple the MNSE system into two sub-problems at each timestep, one is the linear and angular velocities system, the other is the pressure system. Both first-order and second-order projection methods are considered. For the classical first-order projection scheme, the stability and error estimates for the linear and angular velocities and the pressure are established rigorously. In addition, a modified first-order projection scheme which leads to some improved error estimates is also proposed and analyzed. We also present the second-order projection method which is unconditionally stable. Ample numerical experiments are performed to confirm the theoretical predictions and demonstrate the efficiency of the methods.

Analysis of some projection methods for the incompressible fluids with microstructure

Yao-Lin Jiang,Yun-Bo Yang 대한수학회 2018 대한수학회지 Vol.55 No.2

In this article, some projection methods (or fractional-step methods) are proposed and analyzed for the micropolar Navier-Stokes equations (MNSE). These methods allow us to decouple the MNSE system into two sub-problems at each timestep, one is the linear and angular velocities system, the other is the pressure system. Both first-order and second-order projection methods are considered. For the classical first-order projection scheme, the stability and error estimates for the linear and angular velocities and the pressure are established rigorously. In addition, a modified first-order projection scheme which leads to some improved error estimates is also proposed and analyzed. We also present the second-order projection method which is unconditionally stable. Ample numerical experiments are performed to confirm the theoretical predictions and demonstrate the efficiency of the methods.

Molecular Analysis and Expression Patterns of the 14-3-3 Gene Family from Oryza Sativa

Yao, Yuan,Du, Ying,Jiang, Lin,Liu, Jin-Yuan Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.3

The ubiquitous family of 14-3-3 proteins functions as regulators in a variety of physiological processes. Eight rice 14-3-3 genes, designated OsGF14a through h, were identified from an exhaustive search of the genome database. Comparisons of deduced amino acid sequences reveal a high degree of identity among members of the OsGF14 family and reported Arabidopsis 14-3-3 proteins. A phylogenetic study indicates that OsGF14s contain both $\varepsilon$ and non-$\varepsilon$ forms, which is also confirmed by a structural analysis of OsGF14 genes. Furthermore, transcripts of OsGF14b, OsGF14c, OsGF14d, OsGF14e, OsGF14f and OsGF14g were detected in rice tissues. Their different expression patterns, the different effects of environmental stresses and plant hormones on their transcription levels, and the different complementary phenotypes in yeast 14-3-3 mutants not only indicates that OsGF14s are responsive to various stress conditions and regulated by multiple signaling pathways, but also suggests that functional similarity and diversity coexist among the members of OsGF14 family.

Function of VP2 Protein in the Stability of the Secondary Structure of Virus-like Particles of Genogroup II Norovirus at Different pH Levels: Function of VP2 Protein in the Stability of NoV VLPs

Yao Lin,Li Fengling,Wang Lianzhu,Zhai Yuxiu,Jiang Yanhua 한국미생물학회 2014 The journal of microbiology Vol.52 No.11

VP2 is the minor structural protein of noroviruses (NoV)and may function in NoV particle stability. To determinethe function of VP2 in the stability of the NoV particle, weconstructed and purified two kinds of virus-like particles(VLPs), namely, VLPs (VP1) and VLPs (VP1+VP2), fromSf9 cells infected with recombinant baculoviruses by usinga Bac-to-Bac® baculovirus expression system. The two kindsof VLPs were treated with different phosphate buffers (pH2 to pH 8); the secondary structure was then analyzed byfar UV circular dichroism (CD) spectroscopy. Results showedthat significant disruptions of the secondary structure ofproteins were not observed at pH 2 to pH 7. At pH 8, thepercentages of α-helix, β-sheet, and β-turn in VLPs (VP1)were decreased from 11% to 8%, from 37% to 32%, andfrom 20% to 16%, respectively. The percentage of coil wasincreased from 32% to 44%. By contrast, the percentages ofα-helix, β-sheet, and β-turn in VLPs (VP1+VP2) were decreasedfrom 11% to 10%, from 37% to 35%, and from 20%to 19%, respectively. The percentage of coil was increasedfrom 32% to 36%. VLPs (VP1+VP2) was likely more stablethan VLPs (VP1), as indicated by the percentage of the secondarystructures analyzed by CD. These results suggestedthat VP2 could stabilize the secondary structure of VLPsunder alkaline pH conditions. This study provided novelinsights into the molecular mechanism of the function ofVP2 in the stability of NoV particles.

A Design of Mid-wave Infrared Integral Catadioptric Optical System with Wide FOV

Lin yao Yu,Hong guang Jia,Qun Wei,Hu hai Jiang,Tian yi Zhang,Chao Wang 한국광학회 2013 Current Optics and Photonics Vol.17 No.2

In order to deduce the difficulty of fixing the Ritchey-Chretien (R-C) dual reflective optical system and enhance the stability of the secondary mirror, a compact integral structure is presented here composed of two transmitting and two reflective aspheric surfaces. The four surfaces were manufactured from a single germanium lens and integrated together. The two reflective surfaces formed by coating the inner reflecting films were assembled in one lens. It makes the installation of the two mirrors easier and the structure of the secondary mirror more stable. A design of mid-wave infrared (MWIR) compact imaging system is presented with a spectral range chosen as 3.7-4.8 μm. The effective focal length is f=90 mm. The field of view (FOV) for the lens is 4.88°. It has good imaging capability with Modulation Transfer Function (MTF) of all field of view more than 0.55 close to the diffraction limitation. Outdoor experiments were carried out and it is shown that the integral catadioptric optical system performs well on imaging.

Radix ranunculus temate saponins induces apoptosis via the death receptor and mitochondrial pathways in SGC-7901 cells

Mao-lin Li,Han-min Gu,Hong-ya Hang,Yao-li Jiang,Jiao Jiang,Qian-na Gu,Wen-yan Wu,M.-L. Li 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.4

Radix ranunculus temate saponins (RRTS), one of the main constituents extracted from the popular traditional Chinese medicine Radix Ranunculi ternati, has been reported to have various biological activities including anti-cancer effect. The aim of this study is to investigate the effect of RRTS on the cell proliferation and apoptosis in human gastric adenocarcinoma SGC-7901 cells. The data showed that exposure to RRTS for 24 h produced cytotoxic effects on SGC- 7901 cells in a dose-dependent manner (with an IC50 value of 21.22±2.76 μg/mL), which was accompanied by apoptosis induction (from 2.18±0.89% (control) to 63.72±13.16% (100 μg/mL)). Both the extrinsic or death receptor pathway and the intrinsic or mitochondrial pathway were involved in RRTS-induced apoptosis in SGC-7901 cells. Furthermore, apoptotic signaling induced by RRTS was amplified by cross-link between the two pathways via the signal-integrating protein Bid. In conclusion, our findings contribute to better understanding the molecular mechanism of RRTS’ effect on gastric cancer cells and form the basis of the therapeutic development of RRTS in treating gastric cancer in the future.

Molecular Analysis and Expression Patterns of the 14-3-3 Gene Family from Oryza Sativa

( Yuan Yao ),( Ying Du ),( Lin Jiang ),( Jin Yuan Liu ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.3

Comparative Proteomics Analysis of Colorectal Cancer

Wang, Jun-Jiang,Liu, Ying,Zheng, Yang,Lin, Feng,Cai, Guan-Fu,Yao, Xue-Qing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Background and Objective: Protein expression in colon and rectal cancer (CRC) and paired normal tissues was examined by two-dimensional gel electrophoresis (2-DE) to identify differentially expressed proteins. Materials and Methods: Five fresh colorectal cancer and paired adjacent normal tissues were obtained and differentially expressed protein spots were determined using PDQuest software, with identification on the basis of MALDI-TOF mass spectra. Results: Compared with normal colorectal mucosa, protein abnormal expression of 65 spots varying more than 1.5 times were found in 2-DE gels from colorectal cancer samples (P<0.05); forty-two proteins were up-regulated and 23 were down-regulated; twelve protein spots were identified using mass spectrometry, of which 8 were up-regulated, includimng HSPB1and Annexin A4, while 4 were down-regulated, the results being consistent with Western blot findings. Conclusions: Two-dimensional electrophoresis reference maps for CRC tissues and adjacent normal mucosa (NMC) were established and 12 differentially expressed proteins were identified. Up-regulated HSPB1 and Annexin A4 may play many important roles in the pathogenesis of colorectal cancer.

Screening Peptides Binding Specifically to Colorectal Cancer Cells from a Phage Random Peptide Library

Wang, Jun-Jiang,Liu, Ying,Zheng, Yang,Liao, Kang-Xiong,Lin, Feng,Wu, Cheng-Tang,Cai, Guan-Fu,Yao, Xue-Qing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1

The aim of this study was to screen for polypeptides binding specifically to LoVo human colorectal cancer cells using a phage-displayed peptide library as a targeting vector for colorectal cancer therapy. Human normal colorectal mucous epithelial cells were applied as absorber cells for subtraction biopanning with a c7c phage display peptide library. Positive phage clones were identified by enzyme-linked immunosorbent assay and immunofluorescence detection; amino acid sequences were deduced by DNA sequencing. After 3 rounds of screening, 5 of 20 phage clones screened positive, showing specific binding to LoVo cells and a conserved RPM motif. Specific peptides against colorectal cancer cells could be obtained from a phage display peptide library and may be used as potential vectors for targeting therapy for colorectal cancer.

A novel CHD7 variant in a chinese family with CHARGE syndrome

Shan Yanhong,Yao LingFang,Li Linli,Gao Xueping,Jiang Jinghan 한국유전학회 2024 Genes & Genomics Vol.46 No.3

Objective CHARGE syndrome is a rare autosomal dominant (AD) multi-system disorder with a broad and variable clinical manifestation and occurs in approximately 1/10,000 newborns in the world. Mutations in the CHD7 gene are the genetic cause of over 90% of patients with typical CHARGE syndrome. The present study reported a novel variant in the CHD7 gene in a Chinese family with an abnormal fetus. Methods Routine prenatal ultrasound screening showed fetal heart abnormality and left foot varus. Chromosomal microarray analysis (CMA) and fetus-parent whole-exome sequencing (trio-WES) were performed to determine the genetic cause of the fetus. The candidate variant was further verified using Sanger sequencing. Results CMA analysis revealed normal results. However, WES analysis identified a de novo heterozygous variant of c.2919_2922del (NM_017780.4) on exon 11 of CHD7 gene, resulting in a premature truncation of the CHD7 protein (p.Gly975*). The variant was classified as Pathogenic (PVS1 + PS2_Moderate + PM2_Supporting) based on the ACMG guidelines. Combined with the clinical phenotype of fetal heart abnormalities, it was confirmed CHARGE syndrome. Conclusion We identified a novel heterozygous variant c.2919_2922del in CHD7 of a Chinese fetus with CHARGE syndrome, enriching the genotype-phenotype spectrum of CHD7. These results suggest that genetic testing could help facilitate prenatal diagnosis of CHARGE syndrome, thus promoting the appropriate genetic counseling. Objective CHARGE syndrome is a rare autosomal dominant (AD) multi-system disorder with a broad and variable clinical manifestation and occurs in approximately 1/10,000 newborns in the world. Mutations in the CHD7 gene are the genetic cause of over 90% of patients with typical CHARGE syndrome. The present study reported a novel variant in the CHD7 gene in a Chinese family with an abnormal fetus. Methods Routine prenatal ultrasound screening showed fetal heart abnormality and left foot varus. Chromosomal microarray analysis (CMA) and fetus-parent whole-exome sequencing (trio-WES) were performed to determine the genetic cause of the fetus. The candidate variant was further verified using Sanger sequencing. Results CMA analysis revealed normal results. However, WES analysis identified a de novo heterozygous variant of c.2919_2922del (NM_017780.4) on exon 11 of CHD7 gene, resulting in a premature truncation of the CHD7 protein (p.Gly975*). The variant was classified as Pathogenic (PVS1 + PS2_Moderate + PM2_Supporting) based on the ACMG guidelines. Combined with the clinical phenotype of fetal heart abnormalities, it was confirmed CHARGE syndrome. Conclusion We identified a novel heterozygous variant c.2919_2922del in CHD7 of a Chinese fetus with CHARGE syndrome, enriching the genotype-phenotype spectrum of CHD7. These results suggest that genetic testing could help facilitate prenatal diagnosis of CHARGE syndrome, thus promoting the appropriate genetic counseling.