Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites

Guo, Yan-Wu,Guo, Hui-Li,Li, Xing,Huang, Li-Li,Zhang, Bo-Ning,Pang, Xiao-Bin,Liu, Ben-Ye,Ma, Lan-Qing,Wang, Hong 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

In our recent work (Ma et al., in Planta 229(3):457-469, 2009a and 229(4):1077-1086, 2009b), two three-intron type III PKS genes, PcPKS1 and PcPKS2, were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is a three-intron chalcone synthase (CHS) gene, and PcPKS2 is found to be a three-intron benzalacetone synthase (BAS) gene. The regular CHS encoded by a single intron gene have not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3) and a stilbene synthase (STS) gene with three-intron (PcPKS5) were isolated and characterized by functional and phylogenetic analyses. In comparison with PcPKS1, a bifunctional enzyme with both CHS and BAS activity, the enzymatic product of recombinant PcPKS3 was naringenin, bis-noryangonin (BNY) and 4-coumaroyltriacetic acid lactone (CTAL) occurred as side products. The PcPKS5 synthesized resveratrol and a trace amount of naringenin from p-coumaroyl-CoA. To our knowledge, PcPKS5 is the first reported three-intron STS gene in flowering plants. In this work, we speculated that this involved a possible evolutionary route of plant-specific type III PKS superfamily in P. cuspidatum.

PLCE1 Gene in Esophageal Cancer and Interaction with Environmental Factors

Guo, Li-Yan,Zhang, Shen,Suo, Zhen,Yang, Chang-Shuang,Zhao, Xia,Zhang, Guo-An,Hu, Die,Ji, Xing-Zhao,Zhai, Min Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7

Objective: To study the PLCE1 gene rs2274223 polymorphism with regard to esophageal cancer and its interaction with diet, lifestyle, psychological and environmental factors in Southwest Shandong province. Materials and Methods: A case series study (case-case) was conducted. Questionnaire data were collected and 3 ml-5ml venous blood was drawn for DNA extraction among the qualified research subjects. PLCE1 gene polymorphism was detected after PCR amplification of DNA. SPSS 13.0 software was used for statistical analysis of the data. Results: The three genotypes A/A, A/G and G/G PLCE1 gene rs2274223 was 31, 16 and 4 cases, accounting for 60.8%, 31.4%, 0.08% respectively. The difference of three genotypes (AA/GA/GG) proportion between negative and positive family history of patients was statistically significant, ${\chi}^2=6.213$, p=0.045. There was no statistically significant relationship between PLCE1 gene rs2274223 polymorphism and smoking, drinking, ${\chi}^2=0.119$, p=0.998, and ${\chi}^2=1.727$, p=0.786. There was no linkage of the three rs2274223 PLCE1 gene genotypes (AA/GA/GG) proportion with eating fried, pickled, hot, mildew, overnight, smoked, excitant food, eat speed, salt taste or not (p>0.05). or with living environment pollution and nine risk factors of occupational exposure (p>0.05). There was no statistically significant difference in TS scores between different genotype of rs2274223 PLCE1 gene. Conclusions: The PLCE1 rs2274223 polymorphism has a relationship with family history of esophageal cancer, but does not have any significant association with age, gender, smoking, alcohol drinking, food hygiene, eating habits, living around the environment and occupation in cases.

Efficient removal of methylene blue via two-step modification hazelnut shell biochar: Process intensification, kinetics and thermodynamics

Fanhui Guo,Sixi Guo,Yanjie Niu,Guofeng Qiu,Yang Guo,Yan Li,Liqing Chen,Yixin Zhang,Jianjun Wu 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.125 No.-

Porous carbon materials with ultra-high specific surface area and adjustable pore structure characteristicswere prepared from food industry waste hazelnut shells for the adsorption of methylene blue (MB)wastewater by two-step activation by impregnation with ZnCl2 followed by chemical activation withKOH. The Fusso effect, which can reduce the size of MB molecules, was further used to enhance theadsorption of MB on porous carbon. The results show that both HSBC-a and HSBC-a-b have ultra-highspecific surface area (2979.59 m2/g for HSBC-a, 2882.73 m2/g for HSBC-a-b). The mesopore ratio ofHSBC-a-b (Vmeso/Vtotal % = 14.05%) was doubled compared to HSBC-a. It showed an excellent adsorptionperformance of 694.03 mg/g for MB. It showed a fast adsorption kinetics and the adsorbed amountincreased to 882.46 mg/g at 0.1 M NaCl solution. In addition, adsorption processes were studied usingadsorption kinetics and adsorption isotherm model fitting. The results of this research confirm that hazelnutshell is a kind of promising and sustainable porous carbon raw material, and its ultra-high specificsurface area and adjustable pore structure characteristics are favorable for the efficient treatment ofMB from dyeing wastewater. This work could provide potential guidance for the high-value utilizationof waste hazelnut shell biochar.

Activating Transcription Factor 1 is a Prognostic Marker of Colorectal Cancer

Huang, Guo-Liang,Guo, Hong-Qiang,Yang, Feng,Liu, Ou-Fei,Li, Bin-Bin,Liu, Xing-Yan,Lu, Yan,He, Zhi-Wei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

Objective: Identifying cancer-related genes or proteins is critical in preventing and controlling colorectal cancer (CRC). This study was to investigate the clinicopathological and prognostic value of activating transcription factor 1 (ATF1) in CRC. Methods: Protein expression of ATF1 was detected using immunohistochemistry in 66 CRC tissues. Clinicopathological association of ATF1 in CRC was analyzed with chi-square test or Fisher's exact test. The prognostic value of ATF1 in CRC is estimated using the Kaplan-Meier analysis and Cox regression models. Results: The ATF1 protein expression was significantly lower in tumor tissues than corresponding normal tissues (51.5% and 71.1%, respectively, P = 0.038). No correlation was found between ATF1 expression and the investigated clinicopathological parameters, including gender, age, depth of invasion, lymph node status, metastasis, pathological stage, vascular tumoral emboli, peritumoral deposits, chemotherapy and original tumor site (all with P > 0.05). Patients with higher ATF1 expression levels have a significantly higher survival rate than that with lower expression (P = 0.026 for overall survival, P = 0.008 for progress free survival). Multivariate Cox regression model revealed that ATF1 expression and depth of invasion were the predictors of the overall survival (P = 0.008 and P = 0.028) and progress free survival (P = 0.002 and P = 0.005) in CRC. Conclusions: Higher ATF1 expression is a predictor of a favorable outcome for the overall survival and progress free survival in CRC.

Combination of Tumor Volume and Epstein-Barr Virus DNA Improved Prognostic Stratification of Stage II Nasopharyngeal Carcinoma in the Intensity Modulated Radiotherapy Era: A Large-Scale Cohort Study

Qiu-Yan Chen,Shao-Yan Guo,Lin-Quan Tang,Tong-Yu Lu,Bo-Lin Chen,Qi-Yu Zhong,Meng-Sha Zou,Qing-Nan Tang,Wen-Hui Chen,Shan-Shan Guo,Li-Ting Liu,Yang Li,Ling Guo,Hao-Yuan Mo,Rui Sun,Dong-Hua Luo,Chong Zha 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose Little is known about combination of the circulating Epstein-Barr viral (EBV) DNA and tumor volume in prognosis of stage II nasopharyngeal carcinoma (NPC) patients in the intensity modulated radiotherapy (IMRT) era. We conducted this cohort study to evaluate the prognostic values of combining these two factors. Materials and Methods By Kaplan-Meier, we compare the differences of survival curves between 385 patients with different EBV DNA or tumor volume levels, or with the combination of two biomarkers mentioned above. Results Gross tumor volume of cervical lymph nodes (GTVnd, p < 0.001) and total tumor volume (GTVtotal, p < 0.001) were both closely related to pretreatment EBV DNA, while gross tumor volume of nasopharynx (GTVnx, p=0.047) was weakly related to EBV DNA. EBV DNA was significantly correlated with progress-free survival (PFS, p=0.005), locoregional-free survival (LRFS, p=0.039), and distant metastasis-free survival (DMFS, p=0.017), while GTVtotal, regardless of GTVnx and GTVnd, had a significant correlation with PFS and LRFS. The p-values of GTVtotal for PFS and LRFS were 0.008 and 0.001, respectively. According to GTVtotal and pretreatment EBV DNA level, patients were divided into a low-risk group (EBV DNA 0 copy/mL, GTVtotal < 30 cm3; EBV DNA 0 copy/mL, GTVtotal  30 cm3; or EBV DNA > 0 copy/mL, GTVtotal < 30 cm3) and a high-risk group (EBV DNA > 0 copy/mL, GTVtotal  30 cm3). When patients in the low-risk group were compared with those in the high-risk group, 3-year PFS (p=0.003), LRFS (p=0.010), and DMFS (p=0.031) rates were statistically significant. Conclusion Pretreatment plasma EBV DNA and tumor volume were both closely correlated with prognosis of stage II NPC patients in the IMRT era. Combination of EBV DNA and tumor volume can refine prognosis and indicate for clinical therapy.

Synthesis of a Novel Side Chain Liquid Crystalline-Main Chain Liquid Crystalline Block Copolymer by Radical / Cation Transformation Polymerization

Yan Yang,Hai-Qing Guo 한국고분자학회 2012 한국고분자학회 학술대회 연구논문 초록집 Vol.2012 No.4

A Grey Relational Analysis Model of Scientific Research Ability on Music Based on AHP and Its Realization

Guo Xiaoxi,Ni Yan,Wangjia 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.5

In the process of analyzing scientific research ability on music, many problems present, including that indicators are not comprehensive enough, the model has much subjectivity, the evaluation results are not reliable or the value of quantity of indicators has errors. Thus, this paper proposes a grey relational analysis model of scientific research ability on music based on AHP. It selects out dominant indicators and recessive indicators to evaluate software features and hardware features. A multi-layer evaluation index system for scientific research ability on music is established. AHP is introduced to compute the weight of indicators. After standardization of indicators, a multi-scheme grey relational coefficient model and a grey relational degree model are established according to grey theory to evaluate the level of scientific research ability on music. Proved effective by the case study, this model can realize the evaluation of scientific research ability on music on the computer.

A Nationwide Survey of Privatized Firms in China

Yan Guo,Jie Gan,Chenggang Xu 서울대학교 경제연구소 2008 Seoul journal of economics Vol.21 No.2

This descriptive study is based on a nationwide survey of privatization in China. Between 1995 and 2005, close to 100,000 firms with 11.4 trillion RMB in assets were privatized in China. This privatization process encompassed two-thirds of state-owned enterprises and state assets. Privatization in China has created concentrated private ownership and greatly changed corporate governance. After privatization, the state has withdrawn from firms' daily decision making. Soft budget constraints have been substantially hardened. Firms have become more efficient and more profitable.

VM Hotspot Eliminating Method Based on Hot Degree Comprehensive Evaluating

Yan Yong-ming,Guo Jun,Mo Yu-yan,ZhaNG Bin,Han Qing-liang 보안공학연구지원센터(IJGDC) 2014 International Journal of Grid and Distributed Comp Vol.7 No.2

Free Communications : P2 ; The Mutual Impact of Damaged Skin Barrier and Colonization of Staphylococcus Aureus

( Yan Wu ),( Jian Mei Guo ),( Nan Sun ),( Shao Min Zhong ),( Rong Tao ) 한국피부장벽학회 2013 한국피부장벽학회지 Vol.15 No.2

The atopic dermatitis is characterized by disruption of skin barrier and colonization of S.aureus. However, the mutual impact of damaged skin barrier and increased S.aureus has not been fully elucidated. Here we used the SKH-1 hairless mice to establish acute barrier dysfunction model and evaluated the mutual impact of damaged skin barrier function and colonization of S.aureus. Skin barrier was destroyed by repeat tape-stripping. Then 36 SKH-1 mice were divided into 6 groups, namely the control group, barrier impaired group, S. aureus inoculated group, barrier impaired +S. aureus inoculated group, “wrapping group” (referred to barrier impaired +wrapping+S. aureus inoculation) and “moisturizing group” (referred to barrier impaired + moisturizer + S. aureus). S. aureus on the skin were collected after 24 hours for verification and quantify. The physiological parameters related to skin barrier were detected before and immediately after impairing of barrier function, after wrapping/moisturizing, 4 hours and 24 hours after inoculation of S. aureus. In this study, we found that There was a little S. aureus grown on intact skin in only inoculation group, while the number of S. aureus in impaired barrier group increased dramatically (P<0.05); the colonization of S. aureus in both wrapping and moisturizing group decreased obviously compared with the barrier impaired group (P <0.05), Fig1, which mean that restoring the barrier function could greatly reduced the colonization of S. aureus. On the other hand the colonization of S. aureus on impaired barrier could inhibit the recovery of skin barrier function, with higher pH and TEWL compared with other groups in similar time points(P<0.05). The wrapping and moisturizing may eliminate the suppression of barrier function recovery caused by inoculation of S. aureus, Fig 2.