Transcriptome profiling of rubber tree (Hevea brasiliensis) discovers candidate regulators of the cold stress respo

Xiao‑Xiao Gong,Bing‑Yu Yan,Jin Hu,Cui‑Ping Yang,Yi‑Jian Li,Jin‑Ping Liu,Wen‑Bin Liao 한국유전학회 2018 Genes & Genomics Vol.40 No.11

Tropical plant rubber tree (Hevea brasiliensis) is the sole source of commercial natural rubber and low-temperature stress is the most important limiting factor for its cultivation. To characterize the gene expression profiles of H. brasiliensis under the cold stress and discover the key cold stress-induced genes. Three cDNA libraries, CT (control), LT2 (cold treatment at 4 °C for 2 h) and LT24 (cold treatment at 4 °C for 24 h) were constructed for RNA sequencing (RNA-Seq) and gene expression profiling. Quantitative real time PCR (qRT-PCR) was conducted to validate the RNA-Seq and gene differentially expression results. A total of 1457 and 2328 differentially expressed genes (DEGs) in LT2 and LT24 compared with CT were respectively detected. Most significantly enriched KEGG pathways included flavonoid biosynthesis, phenylpropanoid biosynthesis, plant hormone signal transduction, cutin, suberine and wax biosynthesis, Pentose and glucuronate interconversions, phenylalanine metabolism and starch and sucrose metabolism. A total of 239 transcription factors (TFs) were differentially expressed following 2 h or/and 24 h of cold treatment. Cold-response transcription factor families included ARR-B, B3, BES1, bHLH, C2H, CO-like, Dof, ERF, FAR1, G2-like, GRAS, GRF, HD-ZIP, HSF, LBD, MIKC-MADS, M-type MADS, MYB, MYB-related, NAC, RAV, SRS, TALE, TCP, Trihelix, WOX, WRKY, YABBY and ZF-HD. The genome-wide transcriptional response of rubber tree to the cold treatments were determined and a large number of DEGs were characterized including 239 transcription factors, providing important clues for further elucidation of the mechanisms of cold stress responses in rubber tree.

Effect of Prior Microstructures on Cementite Dissolution Behavior During Subcritical Annealing of High Carbon Steels

Xiao‑Yu Zhao,Xian‑Ming Zhao,Chun‑Yu Dong,Yang Yang,Huai‑Bin Han 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.6

The variation of the morphology and distribution of cementite particles in different prior structures with spheroidizingannealing process has been proceeded in this paper. It is found that the dissolution and coarsening progresses of variousinitial structures in spheroidizing annealing process are quite asynchronous due to the different interlamellar spacing. Thedissolution rate of degenerated pearlite (D-P) with finer interlamellar spacing is faster. The granular cementite in the spheroidizedmicrostructure is fine, uniform and dense. The mean diameter of spherical cementite is refined to 233 nm. However,the initial structure of degenerated pearlite (D-P) is highly sensitive to the austenitization temperature. The cementite iseasy to be coarsened under high austenitizing temperature. The coarsening is accompanied by the gradual increase of theCr content in the cementite, which increases the stability of the cementite. Therefore, the optimal austenitizing temperaturefor degenerated pearlite (D-P) is suggested to be 770 °C.

Effect of Trichostatin A on CNE2 Nasopharyngeal Carcinoma Cells - Genome-wide DNA Methylation Alteration

Yang, Xiao-Li,Zhang, Cheng-Dong,Wu, Hua-Yu,Wu, Yong-Hu,Zhang, Yue-Ning,Qin, Meng-Bin,Wu, Hua,Liu, Xiao-Chun,Lina, Xing,Lu, Shao-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Trichostatin A (TSA) is a histone deacetylase (HDAC) inhibitor. We here investigated its effects on proliferation and apoptosis of the CNE2 carcinoma cell line, and attempted to establish genome-wide DNA methylation alteration due to differentially histone acetylation status. After cells were treated by TSA, the inhibitory rate of cell proliferation was examined with a CCK8 kit, and cell apoptosis was determined by flow cytometry. Compared to control, TSA inhibited CNE2 cell growth and induced apoptosis. Furthermore, TSA was found to induce genome-wide methylation alteration as assessed by genome-wide methylation array. Overall DNA methylation level of cells treated with TSA was higher than in controls. Function and pathway analysis revealed that many genes with methylation alteration were involved in key biological roles, such as apoptosis and cell proliferation. Three genes (DAP3, HSPB1 and CLDN) were independently confirmed by quantitative real-time PCR. Finally, we conclude that TSA inhibits CNE2 cell growth and induces apoptosis in vitro involving genome-wide DNA methylation alteration, so that it has promising application prospects in treatment of NPC in vivo. Although many unreported hypermethylated/hypomethylated genes should be further analyzed and validated, the pointers to new biomarkers and therapeutic strategies in the treatment of NPC should be stressed.

EFFECT OF CHARGE DENSITY ON SPRAY CHARACTERISTICS, COMBUSTION PROCESS, AND EMISSIONS OF HEAVY-DUTY DIESEL ENGINES

Bin Yang Wu,Qiang Zhan,Xiao Yang Yu,Wen Yu Gu,Min Zhang,Wanhua Su 한국자동차공학회 2018 International journal of automotive technology Vol.19 No.4

In-cylinder charge density at top dead center is an important parameter of diesel engines and is influenced by intake pressure, intake temperature, and compression ratio. The effects of charge density on fuel spray, combustion process, and emissions were investigated by using a constant volume bomb and a heavy-duty diesel engine. Spray development resistance increased with the increase of the charge density in the constant volume bomb. It was found that short spray penetration was accompanied by a large spray cone angle in the former stage with high charge density. However, the equivalence ratio was lowered and the degree of homogeneity of the mixture was increased in the later stage owing to the rapid interaction of fuel and gas at a high mixing rate. Combining the first law of thermodynamics and the second law of thermodynamics for analysis, as the charge density increased, the gross indicated thermal efficiency (ITEg) was improved. However, pumping loss had to be considered with higher charge density. Under this condition, the brake thermal efficiency (BTE) trend was increased initially and decreased subsequently. Under high-load operation (1200 r/min BMEP, 2.0 MPa), the minimum charge density value of 44.8 kg/m3 was found to be reasonable. This charge density was suitable for combustion and brought about minimum exhaust energy and trade-off emissions. Moreover, by analyzing two operation conditions in terms of the maximum BTE with the Miller and the conventional cycles, compression temperature and combustion temperature were reduced in the Miller cycle with the charge density 44.8 kg/m3. A high C p/C v could improve the cylinder exergy/power conversion process by its positive effect of increasing the specific heat ratio. Owing to the interaction between a high C p/C v and exergy loss to heat transfer, the condition with the minimal charge density could produce more piston work.

魏晉寫槐骈赋研究

Yang Mu Xiao,Yang Xiao Bin 한국중국산문학회 2013 중국산문연구집간 Vol.3 No.-

홰나무를 소재로 한 변문은 위진시기 문인들에게 상당한 인기를 끌었으며 역대로 더욱 발전양상을 띄어 구식, 결구, 장법, 수사 등 여러 분야가 날로 성숙되었다. 홰나무에 대한 상징을 사용하는 일과 그 문화적 내함은 점차로 확립되어 당시의 심미관을 선명하게 체현하였다. 위진시기 홰나무 소재 변문 중 홰 나무 상징의 사용은 대략 세 가지 종류가 있다. 大槐安國(한 사람이 홰나무 밑에서 낮잠을 자다가 꿈에 대괴안국 왕의 사위가 되어 南柯郡을 20년 동안 다스리면서 부귀영화를 누리다가 꿈을 깨었다는 내용)의 정치적 우의, 槐社安民의 심리적 기탁, 그리고 槐仙之道의 현학적 함의이다. 일종의 상징 부호로서 홰나무는 위진시기에는 더 이상 자연지물에 대한 묘사는 아니었다. 부단한 전습과 변화로 다양한 측면의 인문 내함을 부여받게 되었고 농후한 역사적 연속성을 띄게 된 것이다.

Study of the Cavitation Phenomenon Inside Roller Vane Pump

Bin Zhang,Chun-xiao Zhao,Hao-cen Hong,Da-peng Bai,Hua-yong Yang 한국유체기계학회 2021 International journal of fluid machinery and syste Vol.14 No.1

The roller vane pumps are widely used in the automobile industry as steering pumps. However, the cavitation damage near the outlet relief groove could directly decrease serve life and efficiency of the roller vane pump. In this paper, the mechanism of the cavitation is discussed, especially the cavitation damage near the tip of the relief groove. The computational fluid dynamics simulation method (CFD) based on dynamic meshing technique and user defined function (UDF) is applied to calculate flow dynamics and multi-phase flow characteristics, especially the flows inside the fluid film between the stator and valve plate. The simulated results show that the cavitation region is the same with the experiment one, and the cavitation inside the fluid film is caused by the shear action of the stator and valve plate. The negative pressure region near the relief groove has an extreme low pressure at 0.08 MPa, and the maximum flow velocity is 99.3 m/s. Besides, the cavitation region near the relief groove has the highest gas volume fraction of 2.23% and the lowest density of 560 kg/m3. Furthermore, the reason why cavitation happened at this position in a roller vane pump is analyzed by theoretical analysis.

The Lymphatic Drainage Pattern of Internal Mammary Sentinel Lymph Node Identified by Small Particle Radiotracer (99mTc-Dextran 40) in Breast

Xiao-Shan Cao,Guo-Ren Yang,Bin-Bin Cong,Peng-Fei Qiu,Yong-Sheng Wang 대한암학회 2019 Cancer Research and Treatment Vol.51 No.2

Purpose The purpose of this study was to detect the lymphatic drainage pattern of internal mammary area and verify the concept of internal mammary sentinel lymph node (IM-SLN) in breast. Materials and Methods A small particle radiotracer (99mTc-Dextran 40) was prepared and tested. 99mTc-Dextran 40 was injected into intraparenchyma at the sound breast by a modified radiotracer injection technique. Subsequently, dynamic single-photon emission computed tomography (SPECT), computed tomography (CT), and SPECT/CT combination images were performed to identify the radioactive lymph vessels and internal mammary lymph nodes (IMLNs). The direction of lymph drainage and the location of the IMLNs were identified in the SPECT/CT imaging. Results The radiochemical purity of 99mTc-Dextran 40 was > 95%. 99mTc-Dextran 40 could drainage into first, second, and third lymph node and the radioactive lymph node could be detected by the  detector in the animal experiment. After 99mTc-Dextran 40 injecting into intraparenchyma, 50.0% cases (15/30) were identified the drainage lymphatic vessels and radioactive IMLNs by SPECT. The drainage lymphatic vessel was found from injection point to the first IMLN (IM-SLN) after 10.5±0.35 minutes radiotracer injection, and then 99mTc-Dextran 40 was accumulated into the IM-SLN. The combination imaging of SPECT/CT showed the second IMLN received the lymph drainage from the IM-SLN. The lymphatic drainage was step by step in the internal mammary area. Conclusion The lymph was identified to drain from different regions of the breast to IM-SLN, and then outward from IM-SLN to other IMLN consecutively. It demonstrated the concept of the IM-SLN and provided more evidences for the application of internal mammary sentinel lymph node biopsy.

Dry hob with heat transfer enhancement based on field synergy and its temperature field distribution

Xiao Yang,Lang He,Peng Chen,Benjie Li,Yan-bin Du 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.7

Excessive heat accumulation is an essential factor in dry hob wear. Given this problem, this research is conducted on a dry hob with heat transfer enhancement by field synergy (FS-HTE dry hob). Firstly, through the combination of simulation and thermal image reconstruction, the heat accumulation behavior and thermal-induced wear mechanism are investigated for traditional dry hob. Then, using field synergy theory, a three-dimensional solid model is established for FS-HTE dry hob based on the structure of traditional dry hob. Finally, a process experiment by fluid-solid coupling simulation is executed to explore the temperature field distribution law of the FS-HTE dry hob as well as the temperature control mechanism. Results show that the minimum average temperature can be reached for the FS-HTE dry hob characterized by 0° synergy angle. It is also beneficial to suppress the thermal-induced wear benefiting from the characteristics of value and distribution law for the high-temperature region. The research demonstrates a potential method for the thermal-induced wear suppression of dry hob in green dry hobbing.

Enhanced Antibacterial Effect of Polypyrazole-Graphene Oxide Composite

Bin Yang,Yufei Wang,Longqiang Xiao,Xiaowen Hu,Guofu Zhou 한국고분자학회 2017 Macromolecular Research Vol.25 No.1

Besides excellent physical performances, graphene oxide (GO) has been widely acknowledged as a potential antibacterial material. Chemical modification of GO was taken out with polypyrazole by facile amidation in the presence of dicyclohexylcarbodiimide (DCC) and then generated polypyrazole-graphene oxide (FGO) nanohybrid. FGO nanohybrid showed smooth and multilayer structures by scanning electron microscopy. The value of static water contact angle of FGO is 103o, suggesting FGO was a hydrophobic material. Thermo gravimetric analysis of FGO showed a 65% mass loss up 50 to 600 oC, suggesting that FGO is a thermal stability material. Inactivation efficiency test of nominal Escherichia coli cells showed that the viability greatly decreased to 7% after 4 h incubation with FGO under 37 oC, indicating an outstanding antibacterial ability of FGO much better than that of polypyrazole and GO.

Novel Two-dimensional Network Based on Amino-acid-octamolybdate Bridged by Second Metals: {M(H2O)3(pro)Mo4O13}2?H2O (pro = proline, M = Co, Ni, Cu, Zn)

Xiao-Yuan Wu,Can-Zhong Lu*,Chang-Kun Xia,Shu-Mei Chen,Jiu-Hui Liu,Li-Juan Chen,Wen-Bin Yang 대한화학회 2006 Bulletin of the Korean Chemical Society Vol.27 No.8