Two Androstane Derivatives from the Cultures of Fungus Marasmiellus ramealis (Bull.) Singer

Ning-Ning Yang,Qing-Yun Ma,Sheng-Zhuo Huang,Hao-Fu Dai,Zhi-Kai Guo,Zhi-Fang Yu,You-Xing Zhao 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.11

A new androstane derivative, 4β-methyl-15-oxa-14β-androstane-7-ene-4α-carboxylic acid (1) and a known one 4β-methyl-15-oxa-14β-androstane-7-ene-4α-hydroxyl (2) were isolated from the EtOAc extract of the cultures of the fungus Marasmiellus ramealis (Bull.) Singer. Their structures were elucidated on the basis of 1D and 2D NMR as well as MS spectroscopic data analysis. The inhibitory activity of two isolates against acetylcholinesterase (AChE) revealed that compound 1 exhibited definitely inhibitory activity.

Two Androstane Derivatives from the Cultures of Fungus Marasmiellus ramealis (Bull.) Singer

Yang, Ning-Ning,Ma, Qing-Yun,Huang, Sheng-Zhuo,Dai, Hao-Fu,Guo, Zhi-Kai,Yu, Zhi-Fang,Zhao, You-Xing Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.11

A new androstane derivative, $4{\beta}$-methyl-15-oxa-$14{\beta}$-androstane-7-ene-$4{\alpha}$-carboxylic acid (1) and a known one $4{\beta}$-methyl-15-oxa-$14{\beta}$-androstane-7-ene-$4{\alpha}$-hydroxyl (2) were isolated from the EtOAc extract of the cultures of the fungus Marasmiellus ramealis (Bull.) Singer. Their structures were elucidated on the basis of 1D and 2D NMR as well as MS spectroscopic data analysis. The inhibitory activity of two isolates against acetylcholinesterase (AChE) revealed that compound 1 exhibited definitely inhibitory activity.

THE RESEARCH OF BANG BANG-FUZZY CONTROL DECISION

Ning, Yang,Jun, Yang Tie,Lou, Jia Shi 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1

This paper describes the signification, basic principles, system structure and design methods of Bang Bang-Fuzzy control decision, simulating results are given.

Carbon-Ion-Implanted Nd, MgO-Codoped Near-Stoichiometric Lithium-Niobate Optical Waveguides

Ning-Ning Dong,Yang Tan,Yan-Xia Kong,Feng Chen,Hong-Ji Ma 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.55 No.6

Nd, MgO codoped near stoichiometric lithium niobate (Nd:MgO:SLN) is a promising laser gain crystal for infrared laser generations. We report on, to our knowledge for the first time, the planar waveguide formation in Nd:MgO:SLN crystal by MeV carbon ion implantation at dose of 1014 ions/cm2. The guiding properties are investigated by using the well-known m-line technique and end-face arrangement. The refractive index profiles of the waveguides show a typical “well + barrier” distribution, which could confine the light propagation in a non-leaky way. In addition, a numerical simulation of the light propagation shows that the waveguide modal distributions are in good agreement with the experimental data, which means potential applications for the formation of designable devices in Nd:MgO:SLN by using carbon ion implantation. Nd, MgO codoped near stoichiometric lithium niobate (Nd:MgO:SLN) is a promising laser gain crystal for infrared laser generations. We report on, to our knowledge for the first time, the planar waveguide formation in Nd:MgO:SLN crystal by MeV carbon ion implantation at dose of 1014 ions/cm2. The guiding properties are investigated by using the well-known m-line technique and end-face arrangement. The refractive index profiles of the waveguides show a typical “well + barrier” distribution, which could confine the light propagation in a non-leaky way. In addition, a numerical simulation of the light propagation shows that the waveguide modal distributions are in good agreement with the experimental data, which means potential applications for the formation of designable devices in Nd:MgO:SLN by using carbon ion implantation.

Improvement of Enzymatic Stability and Catalytic Efficiency of Recombinant Fusariumoxysporum Trypsin with Different N-Terminal Residues Produced by Pichiapastoris

( Ning Yang ),( Zhenmin Ling ),( Liang Peng ),( Yanlai Liu ),( Pu Liu ),( Kai Zhang ),( Aman ),( Juanjuan Shi ),( Xiangkai Li ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.9

Fusarium oxysporum trypsin (FOT) is a fungal serine protease similar to mammal trypsin. The FOT could be successfully expressed in Pichiapastoris by engineering the natural propeptide APQEIPN. In this study, we constructed two recombinant enzymes with engineered amino acid sequences added to the N-terminus of FOT and expressed in P. pastoris. The N-terminal residues had various effects on the structural and functional properties of trypsin. The FOT, and the recombinants TE (with peptide YVEF) and TS (with peptide YV) displayed the same optimum temperature (40°C) and pH (8.0). However, the combinants TE and TS showed significantly increased thermal stability at 40°C and 50°C. Moreover, the combinants TE and TS also showed enhanced tolerance of alkaline pH conditions. Compared with those of wildtype FOT, the intramolecular hydrogen bonds and the cation π-interactions of the recombinants TE and TS were significantly increased. The recombinants TE and TS also had significantly increased catalytic efficiencies (referring to the specificity constant, k_cat/K_m), 1.75- fold and 1.23-fold than wild-type FOT. In silico modeling analysis uncovered that the introduction of the peptides YVEF and YV resulted in shorter distances between the substrate binding pocket (D174, G198, and G208) and catalytic triad (His42, Asp102, and Ser180), which would improve the electron transfer rate and catalytic efficiency. In addition, N-terminal residues modification described here may be a useful approach for improving the catalytic efficiencies and characteristics of other target enzymes.

Prognostic Role of Nucleophosmin in Colorectal Carcinomas

Yang, Yu-Feng,Zhang, Xi-Ying,Yang, Mei,He, Ze-Hua,Peng, Ning-Fu,Xie, Shu-Rui,Xie, Yan-Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Aim: Recent research suggests that nucleophosmin (NPM) may be a prognostic marker in colorectal carcinomas (CRC). We here tested its use to predict the survival of CRC patients. Methods: We investigated NPM expression by immunohistochemistry in histologically normal to malignant colorectal tissues and evaluated its association with clinicopathological variables. Overall and disease-free survival after tumor removal were calculated by the Kaplan-Meier method, and differences in survival curves were analyzed by the log-rank test. The Cox proportional hazards model was used for multivariate analysis of prognostic factors. Results: NPM expression was found significantly upregulated in CRC compared to adjacent colorectal tissue, villous adenoma, tubular adenoma and normal colorectal mucosa (p<0.05 for all). NPM expression was statistically linked to cancer embolus, lymph node metastasis, differentiation grade, and recurrence of CRC. Overall and disease-free survival of NPM-negative CRC patients tended to be better than those for patients with NPM-positive lesions (log-rank statistic, p<0.05 for all). Multivariate analysis indicated NPM expression as an independent prognostic indicator for CRC patients (p<0.05 ). Conclusion: Our results suggest that NPM expression can predict the survival of CRC patients. Prognosis of CRC is determined by not only many known prognostic factors but also by NPM expression.

Monosomal Karyotypes among 1147 Chinese Patients with Acute Myeloid Leukemia: Prevalence, Features and Prognostic Impact

Yang, Xiao-Fei,Sun, Ai-Ning,Yin, Jia,Cai, Cheng-Sen,Tian, Xiao-Peng,Qian, Jun,Chen, Su-Ning,Wu, De-Pei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

A monosomal karyotype (MK), defined as ${\geq}2$ autosomal monosomies or a single monosomy in the presence of additional structural abnormalities, was recently identified as an independent prognostic factor conveying an extremely poor prognosis in patients with acute myeloid leukemia (AML). In the present study, after excluding patients with t(15;17), t(8;21), inv(16) and normal karyotypes, 324 AML patients with cytogenetic abnormalities were the main subject of analysis. The incidences of MK were 13% in patients aged 15 to 60 years and 18% in those between 15 and 88 years old. MK was much more prevalent among elderly patients (p < 0.001) and was significantly associated with the presence of -7, -5, del(5q), abn12p, abn17p, -18 or 18q-, -20 or 20q- and CK (for all p < 0.001 except for abn12p p=0.009), and +8 or +8q was less frequent in MK+ AML(p=0.007). No correlation was noted between monosomal karyotype and FAB subtype (p > 0.05); MK remained significantly associated with worse overall survival among patients with complex karyotype (p=0.032); A single autosomal monosomy contributed an additional negative effect in OS of patients with structural cytogenetic abnormalities (P=0.008). This report presents the prevalence, feature and prognostic impact of MK among a large series of Chinese AML patients from a single center for the first time.

A FINITE ELEMENT SOLUTION FOR THE CONSERVATION FORM OF BBM-BURGERS' EQUATION

Ning, Yang,Sun, Mingzhe,Piao, Guangri The Youngnam Mathematical Society 2017 East Asian mathematical journal Vol.33 No.5

With the accuracy of the nonlinearity guaranteed, plenty of time and large memory space are needed when we solve the finite element numerical solution of nonlinear partial differential equations. In this paper, we use the Group Element Method (GEM) to deal with the non-linearity of the BBM-Burgers Equation with Conservation form and perform a numerical analysis for two particular initial-boundary value (the Dirichlet boundary conditions and Neumann-Dirichlet boundary conditions) problems with the Finite Element Method (FEM). Some numerical experiments are performed to analyze the error between the exact solution and the FEM solution in MATLAB.

SVM-Based Incremental Learning Algorithm for Large-Scale Data Stream in Cloud Computing

( Ning Wang ),( Yang Yang ),( Liyuan Feng ),( Zhenqiang Mi ),( Kun Meng ),( Qing Ji ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.10

We have witnessed the rapid development of information technology in recent years. One of the key phenomena is the fast, near-exponential increase of data. Consequently, most of the traditional data classification methods fail to meet the dynamic and real-time demands of today`s data processing and analyzing needs--especially for continuous data streams. This paper proposes an improved incremental learning algorithm for a large-scale data stream, which is based on SVM (Support Vector Machine) and is named DS-IILS. The DS-IILS takes the load condition of the entire system and the node performance into consideration to improve efficiency. The threshold of the distance to the optimal separating hyperplane is given in the DS-IILS algorithm. The samples of the history sample set and the incremental sample set that are within the scope of the threshold are all reserved. These reserved samples are treated as the training sample set. To design a more accurate classifier, the effects of the data volumes of the history sample set and the incremental sample set are handled by weighted processing. Finally, the algorithm is implemented in a cloud computing system and is applied to study user behaviors. The results of the experiment are provided and compared with other incremental learning algorithms. The results show that the DS-IILS can improve training efficiency and guarantee relatively high classification accuracy at the same time, which is consistent with the theoretical analysis.

Effect of cell-penetrating peptide-conjugated estrogen-related receptor ${\beta}$ on the development of mouse embryos cultured in vitro

Yang, Ning Jie,Seol, Dong-Won,Jo, Junghyun,Jang, Hyun Mee,Yoon, Sook-Young,Lee, Dong Ryul The Korean Society for Reproductive Medicine 2014 Clinical and Experimental Reproductive Medicine Vol.41 No.1

Objective: Estrogen related receptor ${\beta}$ (Esrrb) is a member of the orphan nuclear receptors and may regulate the expression of pluripotencyrelated genes, such as Oct4 and Nanog. Therefore, in the present study, we have developed a method for delivering exogenous ESRRB recombinant protein into embryos by using cell-penetrating peptide (CPP) conjugation and have analyzed their effect on embryonic development. Methods: Mouse oocytes and embryos were obtained from superovulated mice. The expression of Oct4 mRNA and the cell number of inner cell mass (ICM) in the in vitro-derived and in vivo-derived blastocysts were first analyzed by real time-reverse transcription-polymerase chain reaction and differential staining. Then 8-cell embryos were cultured in KSOM media with or without $2{\mu}g/mL$ CPP-ESRRB protein for 24 to 48 hours, followed by checking their integration into embryos during in vitro culture by Western blot and immunocytochemistry. Results: Expression of Oct4 and the cell number of ICM were lower in the in vitro-derived blastocysts than in the in vivo-derived ones (p<0.05). In the blastocysts derived from the CPP-ESRRB-treated group, expression of Oct4 was greater than in the non-treated groups (p<0.05). Although no difference in embryonic development was observed between the treated and non-treated groups, the cell number of ICM was greater in the CPP-ESRRB-treated group. Conclusion: Treatment of CPP-ESRRB during cultivation could increase embryos' expression of Oct4 and the formation rate of the ICM in the blastocyst. Additionally, an exogenous delivery system of CPP-conjugated protein would be a useful tool for improving embryo culture systems.